公开(公告)号：US08652845B2

公开(公告)日：2014-02-18

申请号：US13635679

申请日：2011-03-18

申请人： Akira Niwa ,  Tatsutoshi Nakahata ,  Toshio Heike

发明人： Akira Niwa ,  Tatsutoshi Nakahata ,  Toshio Heike

IPC分类号： C12N5/095

CPC分类号： C12N5/0647 ,  C12N2501/125 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/415 ,  C12N2506/02

摘要： The present invention provides a method for inducing differentiation of pluripotent stem cells into mesodermal cells, comprising the step of culturing pluripotent stem cells in a serum-free medium without forming an embryoid body and without coculturing with cells from a different species.

摘要翻译： 本发明提供了一种诱导多能干细胞分化为中胚层细胞的方法，包括在无血清培养基中培养多能干细胞而不形成胚状体并且不与来自不同物种的细胞共同培养的步骤。

公开(公告)号：US20130011924A1

公开(公告)日：2013-01-10

申请号：US13635679

申请日：2011-03-18

申请人： Akira Niwa ,  Tatsutoshi Nakahata ,  Toshio Heike

发明人： Akira Niwa ,  Tatsutoshi Nakahata ,  Toshio Heike

IPC分类号： C12N5/078 ,  C12N5/0787 ,  C12N5/077 ,  C12N5/071 ,  C12N5/0789

CPC分类号： C12N5/0647 ,  C12N2501/125 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/415 ,  C12N2506/02

摘要： The present invention provides a method for inducing differentiation of pluripotent stem cells into mesodermal cells, comprising the step of culturing pluripotent stem cells in a serum-free medium without forming an embryoid body and without coculturing with cells from a different species.

摘要翻译： 本发明提供了诱导多能干细胞分化为中胚层细胞的方法，包括在无血清培养基中培养多能干细胞而不形成胚状体并且不与来自不同物种的细胞共同培养的步骤。

公开(公告)号：US08883498B2

公开(公告)日：2014-11-11

申请号：US13642650

申请日：2011-04-21

申请人： Toshio Heike ,  Tatsutoshi Nakahata ,  Tomonari Awaya

发明人： Toshio Heike ,  Tatsutoshi Nakahata ,  Tomonari Awaya

IPC分类号： C12N5/00 ,  C12N5/077 ,  A61K35/12

CPC分类号： C12N5/0658 ,  A61K35/12 ,  C12N2506/02 ,  C12N2506/45

摘要： A method for inducing the differentiation of pluripotent stem cells into skeletal muscle or skeletal muscle progenitor cells is provided. Specifically, a method for producing artificial skeletal muscle or skeletal muscle progenitor cells from human pluripotent stem cells is provided, comprising the following steps of: (1) culturing human pluripotent stem cells by suspension culture; (2) culturing a cell population after suspension culture by adhesion culture; (3) dissociating cells after adhesion culture; and (4) culturing the dissociated cells by adhesion culture. Artificial skeletal muscle or induced skeletal muscle progenitor cells prepared by the method are also provided.

摘要翻译： 提供了诱导多能干细胞分化成骨骼肌或骨骼肌祖细胞的方法。 具体地说，提供从人多能干细胞生产人造骨骼肌或骨骼肌祖细胞的方法，包括以下步骤：（1）通过悬浮培养培养人多能干细胞; （2）通过粘附培养在悬浮培养后培养细胞群; （3）粘附培养后解离细胞; 和（4）通过粘附培养培养解离的细胞。 还提供了通过该方法制备的人造骨骼肌或诱导的骨骼肌祖细胞。

公开(公告)号：US20130040387A1

公开(公告)日：2013-02-14

申请号：US13642650

申请日：2011-04-21

申请人： Toshio Heike ,  Tatsutoshi Nakahata ,  Tomonari Awaya

发明人： Toshio Heike ,  Tatsutoshi Nakahata ,  Tomonari Awaya

IPC分类号： C12N5/0775 ,  C12N5/10

CPC分类号： C12N5/0658 ,  A61K35/12 ,  C12N2506/02 ,  C12N2506/45

摘要： A method for inducing the differentiation of pluripotent stem cells into skeletal muscle or skeletal muscle progenitor cells is provided. Specifically, a method for producing artificial skeletal muscle or skeletal muscle progenitor cells from human pluripotent stem cells is provided, comprising the following steps of: (1) culturing human pluripotent stem cells by suspension culture; (2) culturing a cell population after suspension culture by adhesion culture; (3) dissociating cells after adhesion culture; and (4) culturing the dissociated cells by adhesion culture. Artificial skeletal muscle or induced skeletal muscle progenitor cells prepared by the method are also provided.

摘要翻译： 提供了诱导多能干细胞分化成骨骼肌或骨骼肌祖细胞的方法。 具体地说，提供从人多能干细胞生产人造骨骼肌或骨骼肌祖细胞的方法，包括以下步骤：（1）通过悬浮培养培养人多能干细胞; （2）通过粘附培养在悬浮培养后培养细胞群; （3）粘附培养后解离细胞; 和（4）通过粘附培养培养解离的细胞。 还提供了通过该方法制备的人造骨骼肌或诱导的骨骼肌祖细胞。

公开(公告)号：US09499789B2

公开(公告)日：2016-11-22

申请号：US14001004

申请日：2012-02-23

申请人： Tatsutoshi Nakahata ,  Megumu Saito ,  Akira Niwa ,  Masakatsu Yanagimachi

发明人： Tatsutoshi Nakahata ,  Megumu Saito ,  Akira Niwa ,  Masakatsu Yanagimachi

IPC分类号： C12N5/0784

CPC分类号： C12N5/0639 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/22 ,  C12N2501/26 ,  C12N2506/02 ,  C12N2506/45

摘要： A dendritic cell is produced from pluripotent stem cells by culturing pluripotent stem cells by the following steps: (1) performing adherent culture in a medium which comprises a BMP family protein but does not comprise serum; (2) performing adherent culture in a medium which comprises VEGF but does not comprise serum; (3) performing adherent culture in a medium which comprises a hematopoietic factor but does not comprise serum; and (4) performing suspension culture in a medium which does not comprise serum.

摘要翻译： 通过以下步骤培养多能干细胞从多能干细胞产生树突状细胞：（1）在包含BMP家族蛋白但不包含血清的培养基中进行贴壁培养; （2）在包含VEGF但不包含血清的培养基中进行贴壁培养; （3）在包含造血因子但不包含血清的培养基中进行贴壁培养; 和（4）在不含血清的培养基中进行悬浮培养。

公开(公告)号：US20130038860A1

公开(公告)日：2013-02-14

申请号：US13561820

申请日：2012-07-30

申请人： Eriko Matsui ,  Suguru Dowaki ,  Hirokazu Tatsuta ,  Takeshi Kunihiro ,  Akira Niwa ,  Megumu Saito ,  Tatsutoshi Nakahata

发明人： Eriko Matsui ,  Suguru Dowaki ,  Hirokazu Tatsuta ,  Takeshi Kunihiro ,  Akira Niwa ,  Megumu Saito ,  Tatsutoshi Nakahata

IPC分类号： G01N33/49

CPC分类号： G01N15/05 ,  G01N21/31 ,  G01N21/33 ,  G01N33/49 ,  G01N2021/3144 ,  G01N2021/3155

摘要： A blood analysis apparatus includes an analysis section. The analysis section is configured to compare a measurement spectrum obtained regarding red blood cells with a standard spectrum of heme, biliverdin, and bilirubin, and cause the measurement spectrum to belong to any one of the standard spectra.

摘要翻译： 血液分析装置包括分析部。 分析部将比较红细胞的测定光谱与血红素，胆红素，胆红素的标准光谱进行比较，使测定光谱属于任一标准光谱。

公开(公告)号：US20130330822A1

公开(公告)日：2013-12-12

申请号：US14001004

申请日：2012-02-23

申请人： Tatsutoshi Nakahata ,  Megumu Saito ,  Akira Niwa ,  Masakatsu Yanagimachi

发明人： Tatsutoshi Nakahata ,  Megumu Saito ,  Akira Niwa ,  Masakatsu Yanagimachi

IPC分类号： C12N5/0784

CPC分类号： C12N5/0639 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/22 ,  C12N2501/26 ,  C12N2506/02 ,  C12N2506/45

摘要： A dendritic cell is produced from pluripotent stem cells by culturing pluripotent stem cells by the following steps: (1) performing adherent culture in a medium which comprises a BMP family protein but does not comprise serum; (2) performing adherent culture in a medium which comprises VEGF but does not comprise serum; (3) performing adherent culture in a medium which comprises a hematopoietic factor but does not comprise serum; and (4) performing suspension culture in a medium which does not comprise serum.

摘要翻译： 通过以下步骤培养多能干细胞从多能干细胞产生树突状细胞：（1）在包含BMP家族蛋白但不包含血清的培养基中进行贴壁培养; （2）在包含VEGF但不包含血清的培养基中进行贴壁培养; （3）在包含造血因子但不包含血清的培养基中进行贴壁培养; 和（4）在不含血清的培养基中进行悬浮培养。

公开(公告)号：US08736824B2

公开(公告)日：2014-05-27

申请号：US13561820

申请日：2012-07-30

申请人： Eriko Matsui ,  Suguru Dowaki ,  Hirokazu Tatsuta ,  Takeshi Kunihiro ,  Akira Niwa ,  Megumu Saito ,  Tatsutoshi Nakahata

发明人： Eriko Matsui ,  Suguru Dowaki ,  Hirokazu Tatsuta ,  Takeshi Kunihiro ,  Akira Niwa ,  Megumu Saito ,  Tatsutoshi Nakahata

IPC分类号： G01N33/38 ,  G01N33/49 ,  G01N15/05

CPC分类号： G01N15/05 ,  G01N21/31 ,  G01N21/33 ,  G01N33/49 ,  G01N2021/3144 ,  G01N2021/3155

摘要： A blood analysis apparatus includes an analysis section. The analysis section is configured to compare a measurement spectrum obtained regarding red blood cells with a standard spectrum of heme, biliverdin, and bilirubin, and cause the measurement spectrum to belong to any one of the standard spectra.

摘要翻译： 血液分析装置包括分析部。 分析部将比较红细胞的测定光谱与血红素，胆红素，胆红素的标准光谱进行比较，使测定光谱属于任一标准光谱。

公开(公告)号：US09557321B2

公开(公告)日：2017-01-31

申请号：US13885950

申请日：2011-11-17

申请人： Tatsutoshi Nakahata ,  Megumu Saito ,  Takayuki Tanaka ,  Shinya Yamanaka

发明人： Tatsutoshi Nakahata ,  Megumu Saito ,  Takayuki Tanaka ,  Shinya Yamanaka

IPC分类号： C12N5/00 ,  G01N33/50 ,  C12N5/074 ,  G01N33/68

CPC分类号： G01N33/5055 ,  C12N5/0696 ,  G01N33/6869 ,  G01N33/6872 ,  G01N2333/545 ,  G01N2500/10

摘要： The present invention provides a method for screening drugs for suppressing inflammasome activity, using macrophages derived from induced pluripotent stem cells (iPS cells) having mutant NLRP3 gene.

摘要翻译： 本发明提供了使用衍生自具有突变体NLRP3基因的诱导多能干细胞（iPS细胞）的巨噬细胞来筛选用于抑制炎症性活性的药物的方法。

公开(公告)号：US20150104820A1

公开(公告)日：2015-04-16

申请号：US13990991

申请日：2011-12-02

申请人： Tatsutoshi Nakahata ,  Kohichiro Tsuji ,  Feng Ma ,  Hirohisa Saito ,  Kenji Matsumoto

发明人： Tatsutoshi Nakahata ,  Kohichiro Tsuji ,  Feng Ma ,  Hirohisa Saito ,  Kenji Matsumoto

IPC分类号： C12N5/0787 ,  G01N33/50

CPC分类号： C12N5/0642 ,  C12N5/0696 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/165 ,  C12N2501/20 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/2305 ,  C12N2501/2306 ,  C12N2501/26 ,  C12N2502/02 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/5029 ,  G01N33/5047 ,  G01N33/505 ,  G01N2500/04 ,  G01N2500/10

摘要： The present invention relates to a method for producing human eosinophils from human pluripotent stem cells. More specifically, the present invention provides a method for producing human eosinophils from human pluripotent stem cells, which method comprises the steps of: (1) co-culturing, in the presence of VEGF, human pluripotent stem cells with cells separated from the AGM region of a mammalian fetus; (2) performing suspension culture using a medium comprising IL-3, IL-6, Flt3 ligand, SCF, TPO and serum; (3) performing suspension culture using a medium comprising IL-3, SCF, GM-CSF and serum; and, optionally, (4) performing suspension culture using a medium comprising IL-3, IL-5 and serum.

摘要翻译： 本发明涉及从人多能干细胞生产人嗜酸性粒细胞的方法。 更具体地说，本发明提供从人多能干细胞生产人嗜酸性粒细胞的方法，该方法包括以下步骤：（1）在VEGF的存在下，将人多能干细胞与从AGM区域分离的细胞共培养 的哺乳动物胎儿; （2）使用包含IL-3，IL-6，Flt3配体，SCF，TPO和血清的培养基进行悬浮培养; （3）使用包含IL-3，SCF，GM-CSF和血清的培养基进行悬浮培养; 和（4）使用包含IL-3，IL-5和血清的培养基进行悬浮培养。