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    COFLUORONS AND METHODS OF MAKING AND USING THEM
    4.
    发明申请
    COFLUORONS AND METHODS OF MAKING AND USING THEM 审中-公开
    COFLORONS AND METHODS OF MAKING AND USING THEM

    公开(公告)号：US20140161729A1

    公开(公告)日：2014-06-12

    申请号：US14110019

    申请日：2012-04-09

    申请人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    发明人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    IPC分类号： G01N33/573 ,  C07D405/06 ,  C07D491/107 ,  C07D211/26 ,  C07D263/06 ,  C07F5/02 ,  C07D401/06

    CPC分类号： G01N33/573 ,  C07D211/26 ,  C07D263/06 ,  C07D401/06 ,  C07D405/06 ,  C07D491/107 ,  C07F5/025 ,  C40B30/04 ,  C40B30/06 ,  C40B50/10 ,  C40B50/16

    摘要： The present invention is directed to method of using a collection of monomers capable of forming multimers as a fluorescence reporter in different applications such as ligand detection/screening, disease diagnosis, drug discovery or screening, fluorescent labeling and imaging, or other fluorescent methodologies. Each monomer in the collection includes one or more ligand elements useful for binding to a target molecule with a dissociation constant of less than 300 μM and a linker element connected to the ligand elements directly or indirectly through a connector. Association of linker elements of different combinations of monomers, with their ligand elements bound to the target molecule to form a multimer, will generate a unique fluorescent signature different from that produced by those monomers either alone or in association with each other in the absence of the target molecule, when subjected to electromagnetic excitement.

    摘要翻译： 本发明涉及在不同应用如配体检测/筛选，疾病诊断，药物发现或筛选，荧光标记和成像或其它荧光方法中使用能够形成多聚体的单体的集合的方法。 收集物中的每个单体包括一个或多个配体元件，其用于结合具有小于300μM的解离常数的靶分子，以及通过连接器直接或间接连接到配体元件的连接体元件。 不同单体组合的连接体元件与其与靶分子结合以形成多聚体的连接体元件的关联将产生独特的荧光特征，其不同于单独或彼此相关联的单体，不存在 目标分子，当受到电磁兴奋时。

    Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
    5.
    发明授权
    Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process 有权
    耦合聚合酶链反应 - 限制性内切酶消化 - 连接酶检测反应过程

    公开(公告)号：US07014994B1

    公开(公告)日：2006-03-21

    申请号：US09528014

    申请日：2000-03-17

    申请人： Francis Barany ,  Joseph P. Day ,  Robert P. Hammer ,  Donald E. Bergstrom

    发明人： Francis Barany ,  Joseph P. Day ,  Robert P. Hammer ,  Donald E. Bergstrom

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/683 ,  C12Q1/6816 ,  C12Q1/6853 ,  C12Q2531/137 ,  C12Q2563/179 ,  C12Q2565/125 ,  C12Q2565/501 ,  C12Q2537/149 ,  C12Q2535/125 ,  C12Q2525/155

    摘要： The present invention provides a method for identifying one or more low abundance sequences differing by one or more single-base changes, insertions, or deletions, from a high abundance sequence in a plurality of target nucleotide sequences. The high abundance wild-type sequence is selectively removed using high fidelity polymerase chain reaction analog conversion, facilitated by optimal buffer conditions, to create a restriction endonuclease site in the high abundance wild-type gene, but not in the low abundance mutant gene. This allows for digestion of the high abundance DNA. Subsequently the low abundant mutant DNA is amplified and detected by the ligase detection reaction assay. The present invention also relates to a kit for carrying out this procedure.

    摘要翻译： 本发明提供了一种用于从多个靶核苷酸序列中的高丰度序列鉴定一个或多个不同于一个或多个单碱基变化，插入或缺失的低丰度序列的方法。 使用高保真聚合酶链反应模拟转化，通过最佳缓冲条件促进高丰度野生型序列，在高丰度野生型基因中产生限制性内切核酸酶位点而不是在低丰度突变基因中产生限制性内切核酸酶位点。 这样可以消化高丰度DNA。 随后，通过连接酶检测反应测定扩增和检测低丰度突变体DNA。 本发明还涉及用于实施该方法的试剂盒。

    MONOMERS CAPABLE OF DIMERIZING IN AN AQUEOUS SOLUTION, AND METHODS OF USING SAME
    6.
    发明申请
    MONOMERS CAPABLE OF DIMERIZING IN AN AQUEOUS SOLUTION, AND METHODS OF USING SAME 审中-公开
    可以在水溶液中二维分离的单体及其使用方法

    公开(公告)号：US20140194383A1

    公开(公告)日：2014-07-10

    申请号：US14110056

    申请日：2012-04-09

    申请人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    发明人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    IPC分类号： C07D211/26 ,  C07F5/04

    CPC分类号： C07D211/26 ,  C07D405/06 ,  C07D409/06 ,  C07D491/107 ,  C07F5/025 ,  C07F5/04

    摘要： Described herein are monomers capable of forming a biologically useful multimer when in contact with one, two, three or more other monomers in an aqueous media. In one aspect, such monomers may be capable of binding to another monomer in an aqueous media (e.g. in vivo) to form a multimer, (e.g. a dimer). Contemplated monomers may include a ligand moiety, a linker element, and a connector element that joins the ligand moiety and the linker element. In an aqueous media, such contemplated monomers may join together via each linker element and may thus be capable of modulating one or more biomolecules substantially simultaneously, e.g., modulate two or more binding domains on a protein or on different proteins.

    摘要翻译： 本文描述的是当在水性介质中与一种，两种，三种或更多种其它单体接触时，能够形成生物有用的多聚体的单体。 在一个方面，这样的单体可以能够在水性介质（例如体内）中结合另一单体以形成多聚体（例如二聚体）。 考虑的单体可以包括配体部分，连接体元件和连接配体部分和连接体元件的连接器元件。 在水性介质中，这样预期的单体可以经由每个连接体元件连接在一起，因此可以基本上同时调节一种或多种生物分子，例如调节蛋白质上或不同蛋白质上的两个或多个结合结构域。

    SILYL MONOMERS CAPABLE OF MULTIMERIZING IN AN AQUEOUS SOLUTION, AND METHODS OF USING SAME
    7.
    发明申请
    SILYL MONOMERS CAPABLE OF MULTIMERIZING IN AN AQUEOUS SOLUTION, AND METHODS OF USING SAME 审中-公开
    可在水溶液中多元化的SILYL单体及其使用方法

    公开(公告)号：US20140163229A1

    公开(公告)日：2014-06-12

    申请号：US14110060

    申请日：2012-04-09

    申请人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    发明人： Francis Barany ,  Maneesh Pingle ,  Donald E. Bergstrom ,  Sarah F. Giardina ,  Lee Daniel Arnold

    IPC分类号： C07F7/10

    CPC分类号： A61K31/695 ,  A61K47/55 ,  C07F7/10

    摘要： Described herein are silyl monomers capable of forming a biologically useful multimer when in contact with one, two, three or more other monomers in an aqueous media. Such multimer forming associations of monomers may be promoted by the proximal binding of the monomers to their target biomolecule(s). In one aspect, such monomers may be capable of binding to another monomer in an aqueous media (e.g. in vivo) to form a multimer, (e.g. a dimer). Contemplated monomers may include a ligand moiety, a linker element, and a connector element that joins the ligand moiety and the linker element. In an aqueous media, such contemplated monomers may join together via each linker element and may thus be capable of modulating one or more biomolecules substantially simultaneously, e.g., modulate two or more binding domains on a protein or on different proteins.

    摘要翻译： 本文描述的是当在水性介质中与一种，两种，三种或更多种其它单体接触时能够形成生物学上有用的多聚体的甲硅烷基单体。 单体的这种多聚体形成关联可以通过单体与其目标生物分子的近端结合来促进。 在一个方面，这样的单体可以能够在水性介质（例如体内）中结合另一单体以形成多聚体（例如二聚体）。 考虑的单体可以包括配体部分，连接体元件和连接配体部分和连接体元件的连接器元件。 在水性介质中，这样预期的单体可以经由每个连接体元件连接在一起，因此可以基本上同时调节一种或多种生物分子，例如调节蛋白质上或不同蛋白质上的两个或多个结合结构域。

    APPARATUS AND METHOD OF MAKING AN OFFSET NAIL
    10.
    发明申请
    APPARATUS AND METHOD OF MAKING AN OFFSET NAIL 有权
    装备和制造偏心钉的方法

    公开(公告)号：US20100183403A1

    公开(公告)日：2010-07-22

    申请号：US12644890

    申请日：2009-12-22

    申请人： Zafar I. Ali ,  Donald E. Bergstrom ,  William L. Gabriel

    发明人： Zafar I. Ali ,  Donald E. Bergstrom ,  William L. Gabriel

    IPC分类号： F16B15/02 ,  B21G3/00

    CPC分类号： B21G3/12 ,  F16B15/02 ,  Y10S411/923

    摘要： A nail having an offset head is configured with a multi-step support gusset formed integrally between an undersurface of the offset head and a superior portion of a shank. The head of the nail is relatively thin, and the shank is positioned radially off center of the head. A first portion of the gusset defines an angle relative to the shank axis and the second portion of the gusset defines an angle relative to the shank axis different from that of the first portion. The support gusset reinforces the junction of the shank with the head, supports the toe of the head, and distributes the force of a load on the head through a longitudinal axis of the shank, preventing the toe from bending or yielding and prevents the shank from yielding to the stresses of the load applied.

    摘要翻译： 具有偏移头的钉构造为具有整体形成在偏移头的下表面和柄部的上部之间的多级支撑角撑板。 钉子的头部相对较薄，并且柄部位于头部的中心的径向偏心处。 角撑板的第一部分限定了相对于柄轴线的角度，并且角撑板的第二部分相对于与第一部分的轴线不同的柄轴线限定了一个角度。 支撑角撑板加强了柄与头部的连接处，支撑头部的脚趾，并通过柄的纵向轴线将负载的力分布在头部上，防止脚趾弯曲或屈曲，并防止柄部 屈服于施加的载荷的应力。