公开(公告)号：US07014994B1

公开(公告)日：2006-03-21

申请号：US09528014

申请日：2000-03-17

申请人： Francis Barany ,  Joseph P. Day ,  Robert P. Hammer ,  Donald E. Bergstrom

发明人： Francis Barany ,  Joseph P. Day ,  Robert P. Hammer ,  Donald E. Bergstrom

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/683 ,  C12Q1/6816 ,  C12Q1/6853 ,  C12Q2531/137 ,  C12Q2563/179 ,  C12Q2565/125 ,  C12Q2565/501 ,  C12Q2537/149 ,  C12Q2535/125 ,  C12Q2525/155

摘要： The present invention provides a method for identifying one or more low abundance sequences differing by one or more single-base changes, insertions, or deletions, from a high abundance sequence in a plurality of target nucleotide sequences. The high abundance wild-type sequence is selectively removed using high fidelity polymerase chain reaction analog conversion, facilitated by optimal buffer conditions, to create a restriction endonuclease site in the high abundance wild-type gene, but not in the low abundance mutant gene. This allows for digestion of the high abundance DNA. Subsequently the low abundant mutant DNA is amplified and detected by the ligase detection reaction assay. The present invention also relates to a kit for carrying out this procedure.

摘要翻译： 本发明提供了一种用于从多个靶核苷酸序列中的高丰度序列鉴定一个或多个不同于一个或多个单碱基变化，插入或缺失的低丰度序列的方法。 使用高保真聚合酶链反应模拟转化，通过最佳缓冲条件促进高丰度野生型序列，在高丰度野生型基因中产生限制性内切核酸酶位点而不是在低丰度突变基因中产生限制性内切核酸酶位点。 这样可以消化高丰度DNA。 随后，通过连接酶检测反应测定扩增和检测低丰度突变体DNA。 本发明还涉及用于实施该方法的试剂盒。

公开(公告)号：US07914981B2

公开(公告)日：2011-03-29

申请号：US10854678

申请日：2004-05-25

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US08597890B2

公开(公告)日：2013-12-03

申请号：US13523252

申请日：2012-06-14

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence difference.

摘要翻译： 本发明涉及鉴定靶核苷酸序列的方法。 该方法包括在连接检测反应混合物中的靶核苷酸序列上形成连接产物，扩增连接产物以在聚合酶链式反应（PCR）混合物中形成扩增的连接产物，检测扩增的连接产物，并鉴定靶核苷酸 序列。 连接酶检测反应和聚合酶链式反应的这种偶联允许核酸序列差异的多重检测。

公开(公告)号：US07888009B2

公开(公告)日：2011-02-15

申请号：US10854482

申请日：2004-05-25

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US07312039B2

公开(公告)日：2007-12-25

申请号：US11590056

申请日：2006-10-31

申请人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

发明人： Francis Barany ,  Matthew Lubin ,  George Barany ,  Robert P. Hammer

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6876 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2561/125 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2535/131 ,  C12Q2521/501 ,  C12Q2537/143 ,  C12Q2533/107 ,  C12Q2525/131 ,  C12Q2525/125 ,  C12Q2521/319 ,  C12Q2521/531 ,  C12Q2525/155

摘要： The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.

公开(公告)号：US07083917B2

公开(公告)日：2006-08-01

申请号：US09963920

申请日：2001-09-26

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68 ,  C07H21/02 ,  G01N33/543

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.

公开(公告)号：US06506594B1

公开(公告)日：2003-01-14

申请号：US09526992

申请日：2000-03-16

申请人： Francis Barany ,  Norman P. Gerry ,  Nancy E. Witowski ,  Joseph Day ,  Robert P. Hammer ,  George Barany

发明人： Francis Barany ,  Norman P. Gerry ,  Nancy E. Witowski ,  Joseph Day ,  Robert P. Hammer ,  George Barany

IPC分类号： C12M134

CPC分类号： C12Q1/6837 ,  B01J2219/00313 ,  B01J2219/00326 ,  B01J2219/00416 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00729 ,  C12Q1/6827 ,  C12Q1/6858 ,  C40B40/06 ,  C40B50/08 ,  C40B60/14 ,  C12Q2565/514 ,  C12Q2565/125 ,  C12Q2561/125

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.

公开(公告)号：US08703928B2

公开(公告)日：2014-04-22

申请号：US13229198

申请日：2011-09-09

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C07H21/04 ,  C12N15/11

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US07892747B2

公开(公告)日：2011-02-22

申请号：US12725248

申请日：2010-03-16

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.

公开(公告)号：US07892746B2

公开(公告)日：2011-02-22

申请号：US12725180

申请日：2010-03-16

申请人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

发明人： Francis Barany ,  George Barany ,  Robert P. Hammer ,  Maria Kempe ,  Herman Blok ,  Monib Zirvi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C40B40/06 ,  C40B60/14 ,  Y02A50/58 ,  C12Q2565/501 ,  C12Q2561/125 ,  C12Q2537/143 ,  C12Q2565/514

摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support.