利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

11 条记录 (耗时 0.045 秒)

    METHODS FOR THE IDENTIFICATION OF LRRK2 INTERACTING MOLECULES AND FOR THE PURIFICATION OF LRRK2
    7.
    发明申请
    METHODS FOR THE IDENTIFICATION OF LRRK2 INTERACTING MOLECULES AND FOR THE PURIFICATION OF LRRK2 失效
    LRRK2相互作用分子鉴定方法及LRRK2纯化方法

    公开(公告)号：US20090220992A1

    公开(公告)日：2009-09-03

    申请号：US12282851

    申请日：2007-03-13

    申请人： Gerard Drewes ,  Carsten Hopf ,  Valerie Reader

    发明人： Gerard Drewes ,  Carsten Hopf ,  Valerie Reader

    IPC分类号： G01N33/573 ,  C07D403/04 ,  C12N9/12

    CPC分类号： C12Q1/485 ,  G01N2800/2835

    摘要： The invention provides in a first aspect a method for the identification of an LRRK2 interacting compound, comprising the steps of providing a protein preparation containing LRRK2, contacting the protein preparation with indol ligand 91 immobilized on a solid support under conditions allowing the formation of an indol ligand 91-LRRK2 complex, incubating the indol ligand 91-LRRK2 complex with a given compound, and determining whether the compound is able to separate LRRK2 from the immobilized indol ligand 91. Furthermore, the invention relates to a method for the identification of an LRRK2 interacting compound, comprising the steps of providing a protein preparation containing LRRK2, contacting the protein preparation with indol ligand 91 immobilized on a solid support and with a given compound under conditions allowing the formation of an indol ligand 91-LRRK2 complex, and detecting the indol ligand 91-LRRK2 complex. Additionally, the invention provides a method for the identification of an LRRK2 interacting compound, comprising the steps of providing two aliquots of a protein preparation containing LRRK2, contacting one aliquot with indol ligand 91 immobilized on a solid support under conditions allowing the formation of an indol ligand 91-LRRK2 complex, contacting the other aliquot with indol ligand 91 immobilized on a solid support and with a given compound under conditions allowing the formation of an indol ligand 91-LRRK2 complex, and determining the amount of indol ligand 91-LRRK2 complex. Furthermore, the invention relates to a method for the purification of LRRK2, comprising the steps of providing a protein preparation containing LRRK2, contacting the protein preparation with indol ligand 91 immobilized on a solid support under conditions allowing the formation of an indol ligand 91-LRRK2 complex, and separating LRRK2 from the immobilized indol ligand 91.

    摘要翻译： 本发明在第一方面提供了一种用于鉴定LRRK2相互作用化合物的方法，包括以下步骤：提供含有LRRK2的蛋白质制剂，使所述蛋白质制剂与固定在固体支持物上的吲哚配体91接触，所述条件允许形成吲哚 配体91-LRRK2复合物，将吲哚配体91-LRRK2复合物与给定化合物一起温育，并测定化合物是否能够从固定的吲哚配体91分离LRRK2。此外，本发明涉及用于鉴定LRRK2的方法 包括以下步骤：提供含有LRRK2的蛋白质制剂，使蛋白质制备物与固定在固体载体上的吲哚配体91与允许形成吲哚配体91-LRRK2复合物的条件下的给定化合物接触，并检测吲哚 配体91-LRRK2复合物。 另外，本发明提供了一种用于鉴定LRRK2相互作用化合物的方法，包括以下步骤：提供含有LRRK2的蛋白质制剂的两个等分试样，将一个等分试样与固定在固体支持物上的吲哚配体91接触，形成吲哚 配体91-LRRK2复合物，使其它等分试样与固定在固体载体上的吲哚配体91与给定化合物在允许形成吲哚配体91-LRRK2复合物的条件下接触，并测定吲哚配体91-LRRK2复合物的量。 此外，本发明涉及LRRK2的纯化方法，包括以下步骤：提供含有LRRK2的蛋白质制剂，使蛋白质制剂与固定在固体支持物上的吲哚配体91接触，形成吲哚配体91-LRRK2 复合物，并将LRRK2与固定的吲哚配体91分离。