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公开(公告)号:US20060240505A1
公开(公告)日:2006-10-26
申请号:US11427043
申请日:2006-06-28
申请人: Frank Fan , Yiping He , Jianzhong Huang , Xinhe Jiang , Damien McDevitt , Martin Rosenberg , Annemarie St. John
发明人: Frank Fan , Yiping He , Jianzhong Huang , Xinhe Jiang , Damien McDevitt , Martin Rosenberg , Annemarie St. John
CPC分类号: C12Q1/6897 , C12N15/1034 , C12N15/74 , C12Q1/18 , C12Q1/689 , C12Q2531/113
摘要: A method of determining the cellular or genetic target of an antimicrobial compound includes cloning of a bacterial gene into an expression vector with an inducible promoter and determining whether increasing expression of the cloned gene in the cell will result in resistance to an antimicrobial compound. Also, the method can include incorporation of every gene of a bacterial strain into expression vectors having an inducible promoter, induction, treating with an antimicrobial compound, isolating the gene clone that confers cells resistant to the compound, and determining the identity of the resistant gene by various methods including DNA microarrays and gene sequencing.
摘要翻译: 确定抗微生物化合物的细胞或遗传靶标的方法包括用诱导型启动子将细菌基因克隆到表达载体中,并确定克隆基因在细胞中的表达增加是否会导致对抗微生物化合物的抗性。 此外,该方法可以包括将细菌菌株的每个基因并入具有诱导型启动子的表达载体,诱导,用抗微生物化合物处理,分离赋予细胞对该化合物有抗性的基因克隆,以及确定抗性基因的同一性 通过各种方法,包括DNA微阵列和基因测序。
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公开(公告)号:US06274361B1
公开(公告)日:2001-08-14
申请号:US09080662
申请日:1998-05-18
申请人: Sanjoy Biswas , Martin K. R. Burnham , Andrew P. Fosberry , John E Hodgson , Elizabeth J Lawlor , Tong Li , Damien McDevitt , Christopher Michael Traini , Martin Rosenberg , Judith Ward , Richard Lloyd Warren , Magdalena Zalacain
发明人: Sanjoy Biswas , Martin K. R. Burnham , Andrew P. Fosberry , John E Hodgson , Elizabeth J Lawlor , Tong Li , Damien McDevitt , Christopher Michael Traini , Martin Rosenberg , Judith Ward , Richard Lloyd Warren , Magdalena Zalacain
IPC分类号: C12N916
CPC分类号: C12N9/18
摘要: The invention provides pth polypeptides and polynucleotides encoding pth polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing pth polypeptides to screen for antibacterial compounds.
摘要翻译: 本发明提供第p多肽和编码第p多肽的多核苷酸以及通过重组技术产生此类多肽的方法。 还提供了利用第p多肽筛选抗菌化合物的方法。
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公开(公告)号:US06444208B1
公开(公告)日:2002-09-03
申请号:US09213011
申请日:1998-12-16
IPC分类号: C12N922
CPC分类号: C12N9/22 , A61K38/00 , A61K39/00 , Y10S530/82 , Y10S530/825
摘要: The invention provides rnc polypeptides and DNA (RNA) encoding rnc polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing rnc polypeptides to screen for antibacterial compounds.
摘要翻译: 本发明提供了rnc多肽和编码rnc多肽的DNA(RNA)以及通过重组技术产生此类多肽的方法。 还提供了利用rnc多肽筛选抗菌化合物的方法。
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公开(公告)号:US06348582B1
公开(公告)日:2002-02-19
申请号:US08936165
申请日:1997-09-23
申请人: Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard O Nicholas , Martin Karl Russel Burnham , Julie M Pratt , Martin Rosenberg , Judith M Ward , Michael Arthur Lonetto
发明人: Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard O Nicholas , Martin Karl Russel Burnham , Julie M Pratt , Martin Rosenberg , Judith M Ward , Michael Arthur Lonetto
IPC分类号: C07H2100
CPC分类号: C12N9/0006 , A61K38/00 , A61K39/00 , C07K14/31 , C07K2319/00 , C12N9/00 , C12N9/1205 , C12N9/88 , Y10S435/883
摘要: The invention provides novel polypeptides and polynucleotides encoding such polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing such polypeptides to screen for antibacterial compounds.
摘要翻译: 本发明提供了编码这种多肽的新型多肽和多核苷酸以及通过重组技术产生此类多肽的方法。 还提供了利用这些多肽筛选抗菌化合物的方法。
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公开(公告)号:US06274376B1
公开(公告)日:2001-08-14
申请号:US08923511
申请日:1997-09-04
申请人: Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard Oakley Nicholas , Martin Karl Russel Burnham , Julie M Pratt , Martin Rosenberg , Judith M Ward , Andrew Fosberry , Elizabeth J Lawlor
发明人: Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard Oakley Nicholas , Martin Karl Russel Burnham , Julie M Pratt , Martin Rosenberg , Judith M Ward , Andrew Fosberry , Elizabeth J Lawlor
IPC分类号: C12N1500
CPC分类号: C07K16/1275 , A61K39/00 , C07K14/31 , C12N9/78 , C12Y305/04025
摘要: The invention provides clpL polypeptides and DNA (RNA) encoding clpL polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing clpL polypeptides to screen for antibacterial compounds.
摘要翻译: 本发明提供了clpL多肽和编码clpL多肽的DNA(RNA)和通过重组技术产生这种多肽的方法。 还提供了利用clpL多肽筛选抗菌化合物的方法。
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公开(公告)号:US06264955B1
公开(公告)日:2001-07-24
申请号:US09224502
申请日:1998-12-31
申请人: Julie M Pratt , Martin Rosenberg , Judith M Ward , Michael Arthur Lonetto , Patrick Vernon Warren , Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard O Nicholas , Martin Karl Russel Burnham
发明人: Julie M Pratt , Martin Rosenberg , Judith M Ward , Michael Arthur Lonetto , Patrick Vernon Warren , Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard O Nicholas , Martin Karl Russel Burnham
IPC分类号: A61K3902
CPC分类号: C07K16/1275 , A61K38/00 , A61K39/00 , A61K2039/51 , C07K14/31 , C12N9/78 , C12Y305/04025
摘要: The invention provides Ferrichrome transport ATP-Binding Protein polypeptides and DNA (RNA) encoding Ferrichrome transport ATP-Binding Protein polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing Ferrichrome transport ATP-Binding Protein polypeptides to screen for antibacterial compounds.
摘要翻译: 本发明提供了Ferrichrome转运ATP结合蛋白多肽和编码Ferrichrome转运ATP结合蛋白多肽的DNA(RNA)和通过重组技术产生此类多肽的方法。 还提供了利用Ferrichrome转运ATP结合蛋白多肽筛选抗菌化合物的方法。
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公开(公告)号:US6165462A
公开(公告)日:2000-12-26
申请号:US345603
申请日:1999-06-29
申请人: Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard Oakley Nicholas , Martin Karl Russel Burnham , Julie M Pratt , Martin Rosenberg , Judith M Ward , Michael Arthur Lonetto , Patrick Vernon Warren
发明人: Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard Oakley Nicholas , Martin Karl Russel Burnham , Julie M Pratt , Martin Rosenberg , Judith M Ward , Michael Arthur Lonetto , Patrick Vernon Warren
IPC分类号: G01N33/50 , A61K31/70 , A61K38/00 , A61K38/04 , A61K38/44 , A61K38/55 , A61K39/00 , A61K39/395 , A61K45/00 , A61K48/00 , A61P31/04 , A61P35/00 , A61P37/00 , A61P37/04 , A61P43/00 , C07K14/31 , C07K16/12 , C12N1/00 , C12N1/21 , C12N9/00 , C12N9/04 , C12N9/12 , C12N9/14 , C12N9/88 , C12N15/00 , C12N15/09 , C12N15/31 , C12N15/53 , C12N15/54 , C12P21/02 , C12P21/08 , C12Q1/02 , C12Q1/68 , C12R1/19 , C12R1/44 , C12R1/445 , G01N33/15 , G01N33/53 , G01N33/569 , G01N33/573 , A61K38/51 , C07H21/04 , C12N1/20
CPC分类号: C12N9/0006 , A61K38/00 , A61K39/00 , C07K14/31 , C07K2319/00 , C12N9/00 , C12N9/1205 , C12N9/88 , Y10S435/883
摘要: The invention provides sugar kinase polypeptides and DNA (RNA) encoding sugar kunase polypepties and methods for producing such polypeptides by recombinant techniques. Also provided are methods or utlizng sugar kinase polypeptides to screen for antibacterial compounds.
摘要翻译: 本发明提供糖激酶多肽和编码糖kunase多肽的DNA(RNA),以及通过重组技术产生此类多肽的方法。 还提供了筛选抗菌化合物的方法或使用糖激酶多肽。
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公开(公告)号:US6107071A
公开(公告)日:2000-08-22
申请号:US910505
申请日:1997-08-04
申请人: Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard O Nicholas , Martin Karl Russel Burnham , Julie M Pratt , Martin Rosenberg , Judith M Ward , Michael Arthur Lonetto , Patrick Vernon Warren
发明人: Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard O Nicholas , Martin Karl Russel Burnham , Julie M Pratt , Martin Rosenberg , Judith M Ward , Michael Arthur Lonetto , Patrick Vernon Warren
IPC分类号: G01N33/50 , A61K31/70 , A61K38/00 , A61K38/04 , A61K38/44 , A61K38/55 , A61K39/00 , A61K39/395 , A61K45/00 , A61K48/00 , A61P31/04 , A61P35/00 , A61P37/00 , A61P37/04 , A61P43/00 , C07K14/31 , C07K16/12 , C12N1/00 , C12N1/21 , C12N9/00 , C12N9/04 , C12N9/12 , C12N9/14 , C12N9/88 , C12N15/00 , C12N15/09 , C12N15/31 , C12N15/53 , C12N15/54 , C12P21/02 , C12P21/08 , C12Q1/02 , C12Q1/68 , C12R1/19 , C12R1/44 , C12R1/445 , G01N33/15 , G01N33/53 , G01N33/569 , G01N33/573 , C07H21/04 , C12N1/20
CPC分类号: C12N9/0006 , C07K14/31 , C12N9/00 , C12N9/1205 , C12N9/88 , A61K38/00 , A61K39/00 , C07K2319/00 , Y10S435/883
摘要: The invention provides histidinol dehydrogenase polypeptides and DNA (RNA) encoding histidinol dehydrogenase polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing histidinol dehydrogenase polypeptides to screen for antibacterial compounds.
摘要翻译: 本发明提供组氨醇脱氢酶多肽和编码组氨醇脱氢酶多肽的DNA(RNA),以及通过重组技术产生此类多肽的方法。 还提供了利用组氨醇脱氢酶多肽筛选抗菌化合物的方法。
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公开(公告)号:US6008030A
公开(公告)日:1999-12-28
申请号:US918249
申请日:1997-08-25
申请人: Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard Oakley Nicholas , Martin Karl Russel Burnham , Julie M Pratt , Martin Rosenberg , Judith M Ward , Michael Arthur Lonetto , Patrick Vernon Warren
发明人: Michael Terence Black , John Edward Hodgson , David Justin Charles Knowles , Raymond Winfield Reichard , Richard Oakley Nicholas , Martin Karl Russel Burnham , Julie M Pratt , Martin Rosenberg , Judith M Ward , Michael Arthur Lonetto , Patrick Vernon Warren
IPC分类号: G01N33/50 , A61K31/70 , A61K38/00 , A61K38/04 , A61K38/44 , A61K38/55 , A61K39/00 , A61K39/395 , A61K45/00 , A61K48/00 , A61P31/04 , A61P35/00 , A61P37/00 , A61P37/04 , A61P43/00 , C07K14/31 , C07K16/12 , C12N1/00 , C12N1/21 , C12N9/00 , C12N9/04 , C12N9/12 , C12N9/14 , C12N9/88 , C12N15/00 , C12N15/09 , C12N15/31 , C12N15/53 , C12N15/54 , C12P21/02 , C12P21/08 , C12Q1/02 , C12Q1/68 , C12R1/19 , C12R1/44 , C12R1/445 , G01N33/15 , G01N33/53 , G01N33/569 , G01N33/573 , C12N1/20 , C12P21/06
CPC分类号: C12N9/0006 , C07K14/31 , C12N9/00 , C12N9/1205 , C12N9/88 , A61K38/00 , A61K39/00 , C07K2319/00 , Y10S435/883
摘要: The invention provides sugar kinase polypeptides and DNA (RNA) encoding sugar kinase polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing sugar kinase polypeptides to screen for antibacterial compounds.
摘要翻译: 本发明提供了糖激酶多肽和编码糖激酶多肽的DNA(RNA),以及通过重组技术产生此类多肽的方法。 还提供了利用糖激酶多肽筛选抗菌化合物的方法。
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公开(公告)号:US5891672A
公开(公告)日:1999-04-06
申请号:US977554
申请日:1997-11-25
申请人: Min Wang , Judith M Ward , Richard Lloyd Warren , Richard Oakley Nicholas , Leslie Marie Palmer , Julie M Pratt , David Justin Charles Knowles , Michael Arthur Lonetto , Jeffrey Mooney , Michael Terence Black , Martin Karl Russell Burnham , Christine Debouck , Jason Craig Fedon , John Edward Hodgson , Deborah Dee Jaworski , Raymond Winfield Reichard , Martin Rosenberg , Christopher Michael Traini , Yi Yi Zhong
发明人: Min Wang , Judith M Ward , Richard Lloyd Warren , Richard Oakley Nicholas , Leslie Marie Palmer , Julie M Pratt , David Justin Charles Knowles , Michael Arthur Lonetto , Jeffrey Mooney , Michael Terence Black , Martin Karl Russell Burnham , Christine Debouck , Jason Craig Fedon , John Edward Hodgson , Deborah Dee Jaworski , Raymond Winfield Reichard , Martin Rosenberg , Christopher Michael Traini , Yi Yi Zhong
IPC分类号: G01N33/53 , A61K31/7088 , A61K38/00 , A61K38/43 , A61K39/085 , A61K39/395 , A61K48/00 , A61P1/00 , A61P9/00 , A61P11/00 , A61P11/02 , A61P13/02 , A61P13/12 , A61P17/00 , A61P17/02 , A61P19/00 , A61P25/00 , A61P27/02 , A61P27/16 , A61P43/00 , C07H21/04 , C07K14/31 , C07K16/12 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/78 , C12N9/86 , C12N15/09 , C12N15/31 , C12P21/02 , C12R1/445 , C07H21/00 , C12N15/63
CPC分类号: C12Y305/04025 , C12N9/78 , C07K2319/00
摘要: The invention provides ribA polypeptides and polynucleotides encoding ribA polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing ribA polypeptides to screen for antibacterial compounds.
摘要翻译: 本发明提供ribA多肽和编码ribA多肽的多核苷酸以及通过重组技术产生此类多肽的方法。 还提供了利用ribA多肽筛选抗菌化合物的方法。
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