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37 条记录 (耗时 0.036 秒)

    Clostripain catalyzed amidation of peptides
    5.
    发明授权
    Clostripain catalyzed amidation of peptides 失效
    Clostripain催化肽的酰胺化

    公开(公告)号：US06461834B1

    公开(公告)日：2002-10-08

    申请号：US09212663

    申请日：1998-12-16

    申请人： Dan Dormady ,  Jay S. Stout ,  Daniel J. Strydom ,  Barton Holmquist ,  Fred W. Wagner

    发明人： Dan Dormady ,  Jay S. Stout ,  Daniel J. Strydom ,  Barton Holmquist ,  Fred W. Wagner

    IPC分类号： C12P2106

    CPC分类号： C07K14/605 ,  C12P21/06

    摘要： The invention provides a method of producing a polypeptide having a C-terminal &agr;-carboxamide group. It particularly concerns an enzymatic modification of selected substrate polypeptides which result in cleavage of the substrate polypeptide to form a product peptide with a C-terminal arginine residue having an &agr;-carboxamide group (C-terminal “Arg-NH2”). The method includes contacting an aqueous-based solution including (i) ammonia reagent and (ii) the substrate polypeptide with (iii) clostripain.

    摘要翻译： 本发明提供了产生具有C-末端α-甲酰胺基团的多肽的方法。 它特别涉及所选择的底物多肽的酶修饰，其导致底物多肽切割以形成具有α-甲酰胺基团（C-末端“Arg-NH 2”）的C-末端精氨酸残基的产物肽。 该方法包括使含有（i）氨试剂和（ii）底物多肽的水性溶液与（iii）clostripain接触。

    Monoclonal antibody assay and kit for detecting metal cations in body fluids
    6.
    发明授权
    Monoclonal antibody assay and kit for detecting metal cations in body fluids 失效
    用于检测体液中金属阳离子的单克隆抗体测定和试剂盒

    公开(公告)号：US5532136A

    公开(公告)日：1996-07-02

    申请号：US96671

    申请日：1993-06-22

    申请人： Randall R. Carlson ,  Jay S. Stout ,  Dwane E. Wylie ,  Fred W. Wagner ,  Malcolm Riddell

    发明人： Randall R. Carlson ,  Jay S. Stout ,  Dwane E. Wylie ,  Fred W. Wagner ,  Malcolm Riddell

    IPC分类号： C07K16/00 ,  C07K16/40 ,  C07K16/44 ,  C12Q1/28 ,  G01N33/53 ,  G01N33/543 ,  G01N33/573 ,  G01N33/84

    CPC分类号： C07K16/00 ,  C07K16/40 ,  C07K16/44 ,  G01N33/5308 ,  G01N33/573 ,  G01N33/84

    摘要： The invention provides method and kits for detecting a metallic cation in a sample of a body fluid. The preferred method and kits include the use of at least two different types of antibodies having different specificities. In the preferred method, the sample of body fluid can be contacted with an effective amount of a capture antibody specific for a naturally occurring polypeptide that can bind the metallic cation to form a first antigen-antibody complex. An effective amount of an antibody specific for an epitope on a metallic cation-naturally occurring polypeptide complex or an antibody specific for a metallic cation is added to the first antigen-antibody complex to form a second antigen-antibody complex. The amount of the metallic cation in the sample of body fluid is determined by detecting the amount of the second antigen-antibody complex.

    摘要翻译： 本发明提供了用于检测体液样品中的金属阳离子的方法和试剂盒。 优选的方法和试剂盒包括使用至少两种具有不同特异性的不同类型的抗体。 在优选的方法中，体液样品可以与有效量的天然存在的多肽捕获抗体接触，所述捕获抗体可以结合金属阳离子以形成第一抗原 - 抗体复合物。 将金属阳离子天然存在的多肽复合物或金属阳离子特异性抗体的表位的特异性抗体的有效量加入到第一抗原 - 抗体复合物中以形成第二抗原 - 抗体复合物。 通过检测第二抗原 - 抗体复合物的量来确定体液样品中金属阳离子的量。

    Chemical method for selective modification of the N- and/or C-terminal amino acid .alpha.-carbon reactive group of a recombinant polypeptide or a portion thereof
    7.
    发明授权
    Chemical method for selective modification of the N- and/or C-terminal amino acid .alpha.-carbon reactive group of a recombinant polypeptide or a portion thereof 失效
    用于选择性修饰重组多肽或其部分的N-和/或C-末端氨基酸α-碳反应性基团的化学方法

    公开(公告)号：US5656456A

    公开(公告)日：1997-08-12

    申请号：US457166

    申请日：1995-06-01

    申请人： Jay Stout ,  Fred W. Wagner ,  Thomas R. Coolidge ,  Bart Holmquist

    发明人： Jay Stout ,  Fred W. Wagner ,  Thomas R. Coolidge ,  Bart Holmquist

    IPC分类号： C07K1/00 ,  C07K1/107 ,  C07K1/12 ,  C07K5/11 ,  C07K7/18 ,  C07K14/47 ,  C07K14/475 ,  C12N15/62 ,  C12P21/00

    CPC分类号： C12N15/62 ,  C07K1/003 ,  C07K1/006 ,  C07K1/1072 ,  C07K1/1075 ,  C07K1/12 ,  C07K14/4716 ,  C07K14/475 ,  C07K5/1019 ,  C07K7/18 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/75 ,  Y02P20/55

    摘要： The invention provides for a chemical method for preparing a recombinant single copy polypeptide or a portion thereof with a modified terminal amino acid .alpha.-carbon reactive group selected from the group consisting of N-terminal .alpha.-amine, C-terminal .alpha.-carboxyl, and a combination thereof. The steps of the method involve forming the recombinant single copy polypeptide or a portion thereof so that the single copy polypeptide is protected with one or more biologically added protecting groups at the N-terminal .alpha.-amine, C-terminal .alpha.-carboxyl. The recombinant single copy polypeptide can then be reacted with up to three chemical protecting agents to selectively protect reactive side chain groups and thereby prevent side chain groups from being modified. The recombinant single copy polypeptide can be cleaved with at least one cleavage reagent specific for the biological protecting group to form an unprotected terminal amino acid .alpha.-carbon reactive group. The unprotected terminal amino acid .alpha.-carbon reactive group is modified with at least one chemical modifying agent. The side chain protected terminally modified single copy polypeptide is then deprotected at the side chain groups to form a terminally modified recombinant single copy polypeptide. The number and sequence of steps in the method can be varied to achieve selective modification at the N- and/or C-terminal amino acid of a recombinantly produced polypeptide.

    摘要翻译： 本发明提供了一种用于制备重组单拷贝多肽或其部分的化学方法，其具有修饰的末端氨基酸α-碳反应性基团，其选自N-末端α-胺，C-末端α-羧基和 其组合。 该方法的步骤包括形成重组单拷贝多肽或其部分，使得单拷贝多肽在N-末端α-胺C-末端α-羧基上用一个或多个生物学添加的保护基进行保护。 然后可将重组单拷贝多肽与多达三种化学保护剂反应，以选择性保护反应性侧链基团，从而防止侧链基团被修饰。 重组单拷贝多肽可以用至少一种对生物保护基特异性的切割试剂进行切割以形成未保护的末端氨基酸α-碳反应性基团。 未保护的末端氨基酸α-碳反应性基团用至少一种化学改性剂进行改性。 然后将侧链保护的末端修饰的单拷贝多肽在侧链基团脱保护以形成末端修饰的重组单拷贝多肽。 可以改变方法中的步骤数目和顺序，以实现重组产生的多肽的N-和/或C-末端氨基酸的选择性修饰。

    Monoclonal antibody assay and kit for detecting metal cations in body fluids
    8.
    发明授权
    Monoclonal antibody assay and kit for detecting metal cations in body fluids 失效
    用于检测体液中金属阳离子的单克隆抗体测定和试剂盒

    公开(公告)号：US5620856A

    公开(公告)日：1997-04-15

    申请号：US469489

    申请日：1995-06-06

    申请人： Randall R. Carlson ,  Jay S. Stout ,  Dwane E. Wylie ,  Fred W. Wagner ,  Malcolm Riddell

    发明人： Randall R. Carlson ,  Jay S. Stout ,  Dwane E. Wylie ,  Fred W. Wagner ,  Malcolm Riddell

    IPC分类号： C07K16/00 ,  C07K16/40 ,  C07K16/44 ,  C12Q1/28 ,  G01N33/53 ,  G01N33/543 ,  G01N33/573 ,  G01N33/84

    CPC分类号： C07K16/00 ,  C07K16/40 ,  C07K16/44 ,  G01N33/5308 ,  G01N33/573 ,  G01N33/84

    摘要： The invention provides method and kits for detecting a metallic cation in a sample of a body fluid. The preferred method and kits include the use of at least two different types of antibodies having different specificities. In the preferred method, the sample of body fluid can be contacted with an effective amount of a capture antibody specific for a naturally occurring polypeptide that can bind the metallic cation to form a first antigen-antibody complex. An effective amount of an antibody specific for an epitope on a metallic cation-naturally occurring polypeptide complex or an antibody specific for a metallic cation is added to the first antigen-antibody complex to form a second antigen-antibody complex. The amount of the metallic cation in the sample of body fluid is determined by detecting the amount of the second antigen-antibody complex.

    摘要翻译： 本发明提供了用于检测体液样品中的金属阳离子的方法和试剂盒。 优选的方法和试剂盒包括使用至少两种具有不同特异性的不同类型的抗体。 在优选的方法中，体液样品可以与有效量的天然存在的多肽捕获抗体接触，所述捕获抗体可以结合金属阳离子以形成第一抗原 - 抗体复合物。 将金属阳离子天然存在的多肽复合物或金属阳离子特异性抗体的表位的特异性抗体的有效量加入到第一抗原 - 抗体复合物中以形成第二抗原 - 抗体复合物。 通过检测第二抗原 - 抗体复合物的量来确定体液样品中金属阳离子的量。

    Chemical method for selective modification of the N- and/or C-terminal amino acid .alpha.-carbon reactive group of a recombinant polypeptide or a portion thereof
    9.
    发明授权
    Chemical method for selective modification of the N- and/or C-terminal amino acid .alpha.-carbon reactive group of a recombinant polypeptide or a portion thereof 失效
    用于选择性修饰重组多肽或其部分的N-和/或C-末端氨基酸α-碳反应性基团的化学方法

    公开(公告)号：US5635371A

    公开(公告)日：1997-06-03

    申请号：US294434

    申请日：1994-08-23

    申请人： Jay Stout ,  Fred W. Wagner ,  Thomas R. Coolidge ,  Bart Holmquist

    发明人： Jay Stout ,  Fred W. Wagner ,  Thomas R. Coolidge ,  Bart Holmquist

    IPC分类号： C12N15/09 ,  C07K1/00 ,  C07K1/06 ,  C07K1/107 ,  C07K1/113 ,  C07K1/12 ,  C07K5/11 ,  C07K7/18 ,  C07K14/47 ,  C07K14/475 ,  C07K14/60 ,  C07K19/00 ,  C12N15/62 ,  C12P21/02 ,  C12P21/00

    CPC分类号： C07K14/60 ,  C07K1/003 ,  C07K1/006 ,  C07K1/1072 ,  C07K1/1075 ,  C07K1/12 ,  C07K14/4716 ,  C07K14/475 ,  C07K5/1019 ,  C07K7/18 ,  C12N15/62 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/75 ,  Y02P20/55

    摘要： The invention provides for a chemical method for preparing a recombinant single copy polypeptide or a portion thereof with a modified terminal amino acid .alpha.-carbon reactive group selected from the group consisting of N-terminal .alpha.-amine, C-terminal .alpha.-carboxyl, and a combination thereof. The steps of the method involve forming the recombinant single copy polypeptide or a portion thereof so that the single copy polypeptide is protected with one or more biologically added protecting groups at the N-terminal .alpha.-amine, C-terminal .alpha.-carboxyl. The recombinant single copy polypeptide can then be reacted with up to three chemical protecting agents to selectively protect reactive side chain groups and thereby prevent side chain groups from being modified. The recombinant single copy polypeptide can be cleaved with at least one cleavage reagent specific for the biological protecting group to form an unprotected terminal amino acid .alpha.-carbon reactive group. The unprotected terminal amino acid .alpha.-carbon reactive group is modified with at least one chemical modifying agent. The side chain protected terminally modified single copy polypeptide is then deprotected at the side chain groups to form a terminally modified recombinant single copy polypeptide. The number and sequence of steps in the method can be varied to achieve selective modification at the N- and/or C-terminal amino acid of a recombinantly produced polypeptide.

    摘要翻译： 本发明提供了一种用于制备重组单拷贝多肽或其部分的化学方法，其具有修饰的末端氨基酸α-碳反应性基团，其选自N-末端α-胺，C-末端α-羧基和 其组合。 该方法的步骤包括形成重组单拷贝多肽或其部分，使得单拷贝多肽在N-末端α-胺C-末端α-羧基上用一个或多个生物学添加的保护基进行保护。 然后可将重组单拷贝多肽与多达三种化学保护剂反应，以选择性保护反应性侧链基团，从而防止侧链基团被修饰。 重组单拷贝多肽可以用至少一种对生物保护基特异性的切割试剂进行切割以形成未保护的末端氨基酸α-碳反应性基团。 未保护的末端氨基酸α-碳反应性基团用至少一种化学改性剂进行改性。 然后将侧链保护的末端修饰的单拷贝多肽在侧链基团脱保护以形成末端修饰的重组单拷贝多肽。 可以改变方法中的步骤数目和顺序，以实现重组产生的多肽的N-和/或C-末端氨基酸的选择性修饰。