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公开(公告)号：US20050164376A1
公开(公告)日：2005-07-28
申请号：US11012852
申请日：2004-12-14
申请人： Frederick Balagadde , Carl Hansen , Emil Kartalov , Stephen Quake
发明人： Frederick Balagadde , Carl Hansen , Emil Kartalov , Stephen Quake
IPC分类号： B01L3/00 , C12M1/00 , C12M1/113 , C12M1/18 , C12M3/00 , C12P19/04 , C12P19/34 , C12Q1/02 , C12Q1/68
CPC分类号： C12P19/04 , B01L3/5027 , B01L3/502738 , B01L2300/0861 , B01L2300/0877 , B01L2300/10 , B01L2300/163 , B01L2400/0481 , B01L2400/0655 , C12M23/16 , C12M23/34 , C12M39/00 , C12P1/00
摘要： A chemostat that includes a growth chamber having a plurality of compartments, where each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.
摘要翻译： 一种恒温器，其包括具有多个室的生长室，其中每个室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。 另外，可以防止在恒化器的生长室中形成生物膜的方法。 该方法可以包括以下步骤：将裂解剂加入生长室的分离部分，并将分离的部分与生长室的其余部分重新连接。
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公开(公告)号：US20120065277A1
公开(公告)日：2012-03-15
申请号：US13197654
申请日：2011-08-03
CPC分类号： C12P19/04 , B01L3/5027 , B01L3/502738 , B01L2300/0861 , B01L2300/0877 , B01L2300/10 , B01L2300/163 , B01L2400/0481 , B01L2400/0655 , C12M23/16 , C12M23/34 , C12M39/00 , C12P1/00
摘要： A chemostat is described that includes a growth chamber having a plurality of compartments. Each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat is described. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.
摘要翻译： 描述了一种恒温器，其包括具有多个室的生长室。 每个隔室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。 另外，描述了在恒化器的生长室中防止生物膜形成的方法。 该方法可以包括以下步骤：将裂解剂加入生长室的分离部分，并将分离的部分与生长室的其余部分重新连接。
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公开(公告)号：US20090018195A1
公开(公告)日：2009-01-15
申请号：US12182088
申请日：2008-07-29
CPC分类号： C12P19/04 , B01L3/5027 , B01L3/502738 , B01L2300/0861 , B01L2300/0877 , B01L2300/10 , B01L2300/163 , B01L2400/0481 , B01L2400/0655 , C12M23/16 , C12M23/34 , C12M39/00 , C12P1/00
摘要： A chemostat is described that includes a growth chamber having a plurality of compartments. Each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat is described. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.
摘要翻译： 描述了一种恒温器，其包括具有多个室的生长室。 每个隔室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。 另外，描述了在恒化器的生长室中防止生物膜形成的方法。 该方法可以包括以下步骤：将裂解剂加入生长室的分离部分，并将分离的部分与生长室的其余部分重新连接。
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公开(公告)号：US20140141497A1
公开(公告)日：2014-05-22
申请号：US13850988
申请日：2013-03-26
IPC分类号： C12M1/00
CPC分类号： C12P19/04 , B01L3/5027 , B01L3/502738 , B01L2300/0861 , B01L2300/0877 , B01L2300/10 , B01L2300/163 , B01L2400/0481 , B01L2400/0655 , C12M23/16 , C12M23/34 , C12M39/00 , C12P1/00
摘要： A chemostat is described that includes a growth chamber having a plurality of compartments. Each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat is described. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.
摘要翻译： 描述了一种恒温器，其包括具有多个室的生长室。 每个隔室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。 另外，描述了在恒化器的生长室中防止生物膜形成的方法。 该方法可以包括以下步骤：将裂解剂加入生长室的分离部分，并将分离的部分与生长室的其余部分重新连接。
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公开(公告)号：US08426159B2
公开(公告)日：2013-04-23
申请号：US13197654
申请日：2011-08-03
CPC分类号： C12P19/04 , B01L3/5027 , B01L3/502738 , B01L2300/0861 , B01L2300/0877 , B01L2300/10 , B01L2300/163 , B01L2400/0481 , B01L2400/0655 , C12M23/16 , C12M23/34 , C12M39/00 , C12P1/00
摘要： A chemostat is described that includes a growth chamber having a plurality of compartments. Each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat is described. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.
摘要翻译： 描述了一种恒温器，其包括具有多个室的生长室。 每个隔室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。 另外，描述了在恒化器的生长室中防止生物膜形成的方法。 该方法可以包括以下步骤：将裂解剂加入生长室的分离部分，并将分离的部分与生长室的其余部分重新连接。
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公开(公告)号：US08017353B2
公开(公告)日：2011-09-13
申请号：US12182088
申请日：2008-07-29
CPC分类号： C12P19/04 , B01L3/5027 , B01L3/502738 , B01L2300/0861 , B01L2300/0877 , B01L2300/10 , B01L2300/163 , B01L2400/0481 , B01L2400/0655 , C12M23/16 , C12M23/34 , C12M39/00 , C12P1/00
摘要： A chemostat is described that includes a growth chamber having a plurality of compartments. Each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat is described. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.
摘要翻译： 描述了一种恒温器，其包括具有多个室的生长室。 每个隔室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。 另外，描述了在恒化器的生长室中防止生物膜形成的方法。 该方法可以包括以下步骤：将裂解剂加入生长室的分离部分，并将分离的部分与生长室的其余部分重新连接。
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公开(公告)号：US07407799B2
公开(公告)日：2008-08-05
申请号：US11012852
申请日：2004-12-14
CPC分类号： C12P19/04 , B01L3/5027 , B01L3/502738 , B01L2300/0861 , B01L2300/0877 , B01L2300/10 , B01L2300/163 , B01L2400/0481 , B01L2400/0655 , C12M23/16 , C12M23/34 , C12M39/00 , C12P1/00
摘要： A chemostat is described that includes a growth chamber having a plurality of compartments. Each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat is described. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.
摘要翻译： 描述了一种恒温器，其包括具有多个室的生长室。 每个隔室可以通过一个或多个可致动阀与生长室的其余部分流体隔离。 恒化器还可以包括向生长室供应生长培养基的营养供应线以及从生长室去除流体的输出端口。 另外，描述了在恒化器的生长室中防止生物膜形成的方法。 该方法可以包括以下步骤：将裂解剂加入生长室的分离部分，并将分离的部分与生长室的其余部分重新连接。
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公开(公告)号：US20060019263A1
公开(公告)日：2006-01-26
申请号：US11002479
申请日：2004-12-01
申请人： Stephen Quake , Emil Kartalov
发明人： Stephen Quake , Emil Kartalov
CPC分类号： C12Q1/68 , B01J2219/00398 , B01J2219/00432 , B01J2219/00497 , B01J2219/00527 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00628 , B01J2219/0063 , B01J2219/00632 , B01J2219/00635 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/5027 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2300/0816 , B01L2300/0867 , B01L2300/123 , B01L2400/0481 , B01L2400/0655 , B82Y30/00 , C12Q1/6869 , C12Q2565/629
摘要： Methods for high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention.
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9.发明授权Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension 失效通过异步扩展分析多核苷酸序列的方法和装置公开(公告)号：US07297518B2
公开(公告)日：2007-11-20
申请号：US10099459
申请日：2002-03-12
申请人： Stephen Quake , Ido Braslavsky , Benedict Hebert , Emil Kartalov
发明人： Stephen Quake , Ido Braslavsky , Benedict Hebert , Emil Kartalov
CPC分类号： C12P19/34 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q2535/125 , C12Q2565/629 , C12Q2565/601 , C12Q2563/107
摘要： The invention provides methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension. Some applications of the invention utilize total internal reflection fluorescence microscopy to image polynucleotide molecules at single molecule resolution.
摘要翻译： 本发明提供了通过异步碱基扩展分析多核苷酸序列的方法和装置。 本发明的一些应用利用全内反射荧光显微镜以单分子分辨率对多核苷酸分子进行成像。
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公开(公告)号：US20120141337A1
公开(公告)日：2012-06-07
申请号：US13348495
申请日：2012-01-11
申请人： George Maltezos , John Kim Lee , Axel Scherer , Emil Kartalov
发明人： George Maltezos , John Kim Lee , Axel Scherer , Emil Kartalov
IPC分类号： B01L3/00
CPC分类号： G01N21/6456 , G01N21/6452 , Y10T137/2224
摘要： A microfluidic filter is disclosed. The filter can be used with onchip fluid filtration such as whole blood filtration for microfluidic blood analysis. The filter is able to filter the necessary volume of fluid and in particular blood in an acceptable time frame.
摘要翻译： 公开了一种微流体过滤器。 过滤器可用于片上流体过滤，如全血过滤用于微流体血液分析。 过滤器能够在可接受的时间范围内过滤所需体积的流体，特别是血液。
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