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公开(公告)号：US06727451B1
公开(公告)日：2004-04-27
申请号：US09673034
申请日：2000-11-30
申请人： Günter Fuhr , Rolf Hagedorn , Torsten Müller , Thomas Schnelle , Gabriele Gradl
发明人： Günter Fuhr , Rolf Hagedorn , Torsten Müller , Thomas Schnelle , Gabriele Gradl
IPC分类号： B01D5702
摘要： To manipulate microparticles in a fluid that intersects a first channel or several first channels as a stream, one or more microparticles (14) are exposed to electrical field barriers that change their direction from the direction of flow toward the edge of the flow to a lateral hole (17) of the respective first channel. As a result, microparticles can be moved back and forth between streaming fluids. Preferred applications include treatment, separating, sorting or confinement procedures.
摘要翻译： 为了操纵在与第一通道或多个第一通道相交的流体中的微粒作为流，一个或多个微粒暴露于电场屏障，其将其方向从流动方向朝向流动边缘改变为侧向 （17）。 结果，微粒可以在流动流体之间来回移动。 优选的应用包括处理，分离，分选或限制程序。
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公开(公告)号：US06492175B1
公开(公告)日：2002-12-10
申请号：US09868022
申请日：2001-08-06
IPC分类号： C12N1502
摘要： An electroporation and/or fusion treatment of microscopic objects occurs in a medium between at least two electrodes, with the electrodes being miniaturized electrodes in a microsystem with a channel structure which is set up for the flow-through of the medium with the objects.
摘要翻译： 在至少两个电极之间的介质中发生微观物体的电穿孔和/或融合处理，其中电极是具有通道结构的微系统中的小型化电极，其被设置用于具有物体的介质的流过。
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公开(公告)号：US07501233B2
公开(公告)日：2009-03-10
申请号：US10416278
申请日：2001-11-09
IPC分类号： C12Q1/00
CPC分类号： G01N27/447 , G01N33/48735
摘要： A method is described for non-destructive measurement of vitality of biological cells, especially for determination of apoptosis, in which the at least one cell is exposed to high-frequency alternating, especially rotating, electric fields and/or impedance test fields, and at least one rotation measurement, one dielectrophoresis measurement and/or one impedance measurement is performed with the cell for at least one frequency range or individual frequencies, from which at least one measurement parameter is determined that is characteristic of the vitality state of the cell.
摘要翻译： 描述了一种用于非破坏性测量生物细胞的活力的方法，特别是用于确定细胞凋亡，其中至少一个细胞暴露于高频交替，特别是旋转的电场和/或阻抗测试领域，以及在 对于至少一个频率范围或单独的频率，用该小区执行至少一次旋转测量，一次介电电泳测量和/或一个阻抗测量，从该单元测量至少一个测量参数是该单元的活力状态的特征。
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公开(公告)号：US20060152708A1
公开(公告)日：2006-07-13
申请号：US10544423
申请日：2004-02-04
申请人： Torsten Muller , Stefan Hummel , Annette Pfennig , Gabriele Gradl , Axel Bonsen , Thomas Schnelle , Rudiger Meyer
发明人： Torsten Muller , Stefan Hummel , Annette Pfennig , Gabriele Gradl , Axel Bonsen , Thomas Schnelle , Rudiger Meyer
IPC分类号： G01N21/00
CPC分类号： G01N15/1459 , C12M47/04 , G01N15/1056 , G01N15/12 , G01N15/147 , G01N15/1484 , G01N2015/1006 , G01N2015/1037 , G01N2015/1081 , G01N2015/1477 , G01N2015/149 , G01N2015/1497 , G01N2035/00158
摘要： Disclosed is a method for measuring properties of particles that move through a fluidic microsystem, the particles being suspended in a liquid. According to the inventive method, a first parameter of a specific particle is first measured at a first test station (11). A second parameter of the particle is then measured at an interval from the first measurement at a second test station (12) which is spatially separated from the first test station (11), and the first and second parameters are jointly evaluated in a correlated manner, the first and second parameters being characteristic of different properties of the tested particle. The evaluation comprises a comparison of the first and second parameters with predetermined expected values. Another measurement is taken or manipulations are performed on the tested particle according to the result of the comparison. Also disclosed is a fluidic microsystem that is designed for implementing the inventive method.
摘要翻译： 公开了一种用于测量移动通过流体微系统的颗粒的性质的方法，所述颗粒悬浮在液体中。 根据本发明的方法，首先在第一测试台（11）测量特定颗粒的第一参数。 然后在与第一测试站（11）在空间上分离的第二测试站（12）以与第一测量的间隔的间隔测量颗粒的第二参数，并且以相关方式联合评估第一和第二参数 ，第一和第二参数是测试颗粒的不同性质的特征。 评估包括第一和第二参数与预定预期值的比较。 根据比较的结果，对测试的颗粒进行另一测量或操作。 还公开了被设计用于实施本发明方法的流体微系统。
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公开(公告)号：US20080113333A1
公开(公告)日：2008-05-15
申请号：US11854792
申请日：2007-09-13
申请人： Gabriele Gradl
发明人： Gabriele Gradl
IPC分类号： C12Q1/00
CPC分类号： G01N33/54393
摘要： A method for the prevention of the adhesion of particles, in particular cells and cellular components in solution to surfaces, characterized in that to the solution is added at least one polyalcohol.
摘要翻译： 用于防止颗粒，特别是溶液中的细胞和细胞组分粘附到表面上的方法，其特征在于向溶液中加入至少一种多元醇。
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公开(公告)号：US07300793B2
公开(公告)日：2007-11-27
申请号：US10483218
申请日：2002-07-17
申请人： Gabriele Gradl
发明人： Gabriele Gradl
IPC分类号： C12N5/00
CPC分类号： G01N33/54393
摘要： A method for the prevention of the adhesion of particles, in particular cells and cellular components in solution to surfaces, characterized in that to the solution is added at least one polyalcohol.
摘要翻译： 用于防止颗粒，特别是溶液中的细胞和细胞组分粘附到表面上的方法，其特征在于向溶液中加入至少一种多元醇。
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7.发明申请Multiparametric cell identification and sorting method and associated device 审中-公开多参数细胞识别和分选方法及相关装置公开(公告)号：US20060139638A1
公开(公告)日：2006-06-29
申请号：US10544463
申请日：2004-02-04
申请人： Torsten Muller , Stefan Hummel , Annette Pfennig , Gabriele Gradl , Axel Bonsen , Rudiger Meyer
发明人： Torsten Muller , Stefan Hummel , Annette Pfennig , Gabriele Gradl , Axel Bonsen , Rudiger Meyer
CPC分类号： G01N15/1459 , C12M47/04 , G01N15/1056 , G01N15/12 , G01N15/147 , G01N15/1484 , G01N2015/1006 , G01N2015/1037 , G01N2015/1081 , G01N2015/1477 , G01N2015/149 , G01N2015/1497 , G01N2035/00158
摘要： The invention relates to an method of analysing biological particles, in particular to be conducted in a cell sorter, with following steps: placing the particles to be analysed into a carrier flow, carrying out a first analysis of the particles moving with the carrier flow, selecting at least one particle in dependence on the result of the first analysis, carrying out a second analysis of the selected particle in a decelerated condition. Furthermore, the invention comprises a corresponding analysis device.
摘要翻译： 本发明涉及一种分析生物颗粒的方法，特别是在细胞分选器中进行分析，其步骤如下：将要分析的颗粒放入载体流中，对载体流动的颗粒进行第一次分析， 根据第一次分析的结果选择至少一个粒子，在减速条件下进行所选粒子的第二次分析。 此外，本发明包括相应的分析装置。
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