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公开(公告)号：US20170205404A1
公开(公告)日：2017-07-20
申请号：US15366520
申请日：2016-12-01
Applicant: GENERAL ELECTRIC COMPANY
IPC: G01N33/543 , G01N33/569
CPC classification number: G01N33/54326 , B01L3/00 , B01L3/50273 , B01L3/502753 , B01L2200/0668 , B01L2200/141 , B01L2300/0829 , B01L2300/0851 , B01L2400/043 , G01N33/505 , G01N33/54346 , G01N33/56972
Abstract: Described are multi-functional beads and methods to capture rare cells directly from low-volume biological samples and perform both functional and genomic assays from those cells. This is accomplished using a multifunctional capture bead that allows co-localization of both the single cell capture element and the molecular assay components. When combined with a digital microfluidic platform this enables encoding and/or barcoding of specific single cells.
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2.发明申请SUBSTRATES AND METHODS FOR COLLECTION, STABILIZATION AND ELUTION OF BIOMOLECULES 有权生物分子收集，稳定和提取的基质和方法公开(公告)号：US20140234942A1
公开(公告)日：2014-08-21
申请号：US14261900
申请日：2014-04-25
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Ernest William Kovacs , Erik Leeming Kvam , Bing Li , Frank John Mondello
CPC classification number: B01J20/28038 , B01J20/22 , B01J20/24 , B01J20/28033 , B01J20/3212 , B01J20/3242 , B01J20/3246 , C07K1/145 , C07K1/16 , C07K14/525 , C07K14/5421 , C07K14/545 , C07K14/775 , C12N9/2471 , C12N15/1006 , C12N15/1017 , C12Y302/01023
Abstract: A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described.
Abstract translation: 提供了用于提取，稳定和储存蛋白质的固体底物。 底物包括：多糖，例如基本干燥状态下的松三草。 底物被配置为从样品中提取蛋白质并在环境条件下以干燥形式稳定所提取的蛋白质较长时间。 还描述了收集和回收储存在干固体基质中的蛋白质的方法。
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公开(公告)号：US10294454B2
公开(公告)日：2019-05-21
申请号：US15245584
申请日：2016-08-24
Applicant: General Electric Company
Inventor： Ernest William Kovacs , Anup Sood , Reginald Donovan Smith , Evelina Roxana Loghin , Padmaparna Chadhuri , Vandana Keskar , Chrystal Mae Chadwick , Martin James Brown
IPC: A61K38/00 , C12N5/00 , C12N5/02 , C12N5/0783 , C07K16/28
Abstract: Provided are methods and kits for activating T cells, the method comprising providing a population of T cells, adding a plurality of first agents, where the first agent comprises a T-cell activator and a first binder moiety, and adding a second agent comprising a plurality of capture oligomers, where at least a segment of at least one of the plurality of capture oligomers is capable of associating with the first binder moiety. The method further comprises incubating the population of T cells, whereby at least a portion of the population of T cells is activated.
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公开(公告)号：US20170267716A1
公开(公告)日：2017-09-21
申请号：US15071210
申请日：2016-03-16
Applicant: General Electric Company
Inventor： Anthony John Murray , Andrew David Pris , Erik Leeming Kvam , Nandini Nagraj , Eugene Pauling Boden , Ernest William Kovacs , Louisa Ruth Carr , Rui Chen , Tiberiu Mircea Siclovan , Kelly Scott Chichak
IPC: C07K1/36 , C07K16/00 , C12N9/74 , C12N15/115 , C07K14/705
CPC classification number: C07K1/36 , C07K1/32 , C07K14/70564 , C07K16/4291 , C12N9/6429 , C12Y304/21005
Abstract: Provided herein are aptamer-polymer conjugates which are responsive to environmental stimuli and are useful in selective purification of untagged target polypeptides.
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5.发明申请SUBSTRATES AND METHODS FOR COLLECTION, STABILIZATION AND ELUTION OF BIOMOLECULES 审中-公开生物分子收集，稳定和提取的基质和方法公开(公告)号：US20170021333A1
公开(公告)日：2017-01-26
申请号：US15285986
申请日：2016-10-05
Applicant: General Electric Company
Inventor： Ernest William Kovacs , Frank John Mondello , Erik Leeming Kvam , Bing LI
IPC: B01J20/28 , C12N9/38 , C07K14/775 , C07K1/16 , C07K14/54 , C07K14/525 , C07K1/14 , B01J20/24 , C07K14/545
CPC classification number: B01J20/28038 , B01J20/22 , B01J20/24 , B01J20/28033 , B01J20/3212 , B01J20/3242 , B01J20/3246 , C07K1/145 , C07K1/16 , C07K14/525 , C07K14/5421 , C07K14/545 , C07K14/775 , C12N9/2471 , C12N15/1006 , C12N15/1017 , C12Y302/01023
Abstract: A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described.
Abstract translation: 提供了用于提取，稳定和储存蛋白质的固体底物。 底物包括：多糖，例如基本干燥状态下的松三草。 底物被配置为从样品中提取蛋白质并在环境条件下以干燥形式稳定所提取的蛋白质较长时间。 还描述了收集和回收储存在干固体基质中的蛋白质的方法。
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Patent Agency Ranking
公开(公告)号：US10450345B2
公开(公告)日：2019-10-22
申请号：US15619148
申请日：2017-06-09
Applicant: General Electric Company
Inventor： Erik Leeming Kvam , Bruce Allan Hay , Ernest William Kovacs
IPC: C07K1/36 , C08L71/02 , C07K16/00 , B01D11/04 , C12N15/11 , B01D17/02 , C07K1/14 , B01J20/26 , B01D15/38 , C12N15/115
Abstract: A method for purification of a target in a solution includes providing a target in a solution, the solution comprising one or more contaminants, providing a polymer conjugate in the solution, wherein the polymer conjugate is configured to specifically bind the target, incubating the solution at a first temperature to facilitate binding of the polymer conjugate to the target, providing an environmentally-responsive block copolymer in the solution, wherein the environmentally-responsive block copolymer comprises one or more of an ethylene oxide (EO), a propylene oxide (PO), or an EO/PO block copolymer, and heating the solution comprising the environmentally-responsive block copolymer and the polymer conjugate bound to the target at a second temperature to initiate a liquid-liquid phase separation, wherein the liquid-liquid phase separation produces an aqueous phase and a liquid polymer phase, and wherein the liquid polymer phase comprises the polymer conjugate bound to the target.
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公开(公告)号：US20180057791A1
公开(公告)日：2018-03-01
申请号：US15245584
申请日：2016-08-24
Applicant: General Electric Company
Inventor： Ernest William Kovacs , Anup Sood , Reginald Donovan Smith , Evelina Roxana Loghin , Padmaparna Chadhuri , Vandana Keskar , Chrystal Mae Chadwick , Martin James Brown
IPC: C12N5/0783 , C07K16/28
CPC classification number: C12N5/0636 , C07K16/2809 , C07K16/2818 , C12N2501/51 , C12N2501/515
Abstract: Provided are methods and kits for activating T cells, the method comprising providing a population of T cells, adding a plurality of first agents, where the first agent comprises a T-cell activator and a first binder moiety, and adding a second agent comprising a plurality of capture oligomers, where at least a segment of at least one of the plurality of capture oligomers is capable of associating with the first binder moiety. The method further comprises incubating the population of T cells, whereby at least a portion of the population of T cells is activated.
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8.发明授权Substrates and methods for collection, stabilization and elution of biomolecules 有权用于收集，稳定和洗脱生物分子的底物和方法公开(公告)号：US09480966B2
公开(公告)日：2016-11-01
申请号：US14261900
申请日：2014-04-25
Applicant: General Electric Company
Inventor： Ernest William Kovacs , Erik Leeming Kvam , Bing Li , Frank John Mondello
CPC classification number: B01J20/28038 , B01J20/22 , B01J20/24 , B01J20/28033 , B01J20/3212 , B01J20/3242 , B01J20/3246 , C07K1/145 , C07K1/16 , C07K14/525 , C07K14/5421 , C07K14/545 , C07K14/775 , C12N9/2471 , C12N15/1006 , C12N15/1017 , C12Y302/01023
Abstract: A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described.
Abstract translation: 提供了用于提取，稳定和储存蛋白质的固体底物。 底物包括：多糖，例如基本干燥状态下的松三草。 底物被配置为从样品中提取蛋白质并在环境条件下以干燥形式稳定所提取的蛋白质较长时间。 还描述了收集和回收储存在干固体基质中的蛋白质的方法。
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公开(公告)号：US11512288B2
公开(公告)日：2022-11-29
申请号：US16339077
申请日：2017-08-24
Applicant: General Electric Company
Inventor： Ernest William Kovacs , Anup Sood , Reginald Donovan Smith , Evelina Roxana Loghin , Padmaparna Chaudhuri , Vandana Keskar , Chrystal Mae Chadwick , Martin James Brown
IPC: C12N5/0783 , C07K16/28
Abstract: Provided herein are methods of activating immune cells. The method includes providing a population of immune cells and contacting the population of immune cells with a first agent and a second agent. The first agent includes an immune cell activator attached to a first binder moiety, and the second agent includes at least one capture oligomer. The at least one capture oligomer is capable of associating with the first binder moiety. Also provided are kits for activating immune cells.
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公开(公告)号：US20200181572A1
公开(公告)日：2020-06-11
申请号：US16339077
申请日：2017-08-24
Applicant: General Electric Company
Inventor： Ernest William Kovacs , Anup Sood , Reginald Donovan Smith , Evelina Roxana Loghin , Padmaparna Chaudhuri , Vandana Keskar , Chrystal Mae Chadwick , Martin James Brown
IPC: C12N5/0783 , C07K16/28
Abstract: Provided herein are methods of activating immune cells. The method includes providing a population of immune cells and contacting the population of immune cells with a first agent and a second agent. The first agent includes an immune cell activator attached to a first binder moiety, and the second agent includes at least one capture oligomer. The at least one capture oligomer is capable of associating with the first binder moiety. Also provided are kits for activating immune cells.
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