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1.发明授权公开(公告)号：US08075694B2
公开(公告)日：2011-12-13
申请号：US12485767
申请日：2009-06-16
CPC分类号： C12N9/58 , A23L13/424 , A23L33/18 , C12N9/48 , C12P7/06 , C12P7/10 , C12P19/02 , Y02E50/16 , Y02E50/17
摘要： The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.
摘要翻译： 本发明涉及生产乙醇的方法，并且在无烹饪发酵中减少残余淀粉的产生，包括在合适的发酵条件下，在低于淀粉糊化温度的温度下，使含有颗粒淀粉的底物与颗粒状淀粉水解酶，蛋白酶和发酵微生物接触 淀粉底物的温度以产生乙醇，其中乙醇生产增加，并且残余淀粉的量与基本上相似的不含蛋白酶的方法相比降低。
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2.发明授权公开(公告)号：US07563607B2
公开(公告)日：2009-07-21
申请号：US11314987
申请日：2005-12-21
IPC分类号： C12P7/06
CPC分类号： C12N9/58 , A23L13/424 , A23L33/18 , C12N9/48 , C12P7/06 , C12P7/10 , C12P19/02 , Y02E50/16 , Y02E50/17
摘要： The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.
摘要翻译： 本发明涉及生产乙醇的方法，并且在无烹饪发酵中减少残余淀粉的产生，包括在合适的发酵条件下，在低于淀粉糊化温度的温度下，使含有颗粒淀粉的底物与颗粒状淀粉水解酶，蛋白酶和发酵微生物接触 淀粉底物的温度以产生乙醇，其中乙醇生产增加，并且残余淀粉的量与基本上相似的不含蛋白酶的方法相比降低。
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公开(公告)号：US20110165288A1
公开(公告)日：2011-07-07
申请号：US10587006
申请日：2005-02-17
申请人： Gang Duan , Nigel Dunn-Coleman , Jayarama K. Shetty , Alex Sung , Ying Qian
发明人： Gang Duan , Nigel Dunn-Coleman , Jayarama K. Shetty , Alex Sung , Ying Qian
CPC分类号： A23J1/125 , A23J3/346 , A23K20/147 , A23L7/107 , A23L7/198
摘要： The present invention relates to a process for producing a rice protein concentrate from a rice substrate comprising, enzymatically hydrolyzing a rice substrate with an enzyme having granular starch hydrolyzing (GSH) activity and a second starch hydrolyzing enzyme at a temperature at or below 72° C. and at a pH of about 3.0 to 6.5 to obtain a solubilized starch fraction and a residue fraction which includes insoluble protein, and separating the solubilized starch fraction from the residue to obtain a rice protein concentrate. The rice protein concentrate may be used in animal feed and human food formulations.
摘要翻译： 本发明涉及一种从水稻基质制备大米蛋白浓缩物的方法，包括在72℃或低于72℃的温度下，用具有颗粒状淀粉水解（GSH）活性的酶和第二淀粉水解酶酶水解水稻基质 并且在约3.0至6.5的pH下，得到溶解的淀粉级分和包含不溶性蛋白质的残余物级分，并将残留的溶解的淀粉部分分离，得到大米蛋白质浓缩物。 米蛋白浓缩物可用于动物饲料和人类食物制剂中。
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公开(公告)号：US20060154353A1
公开(公告)日：2006-07-13
申请号：US11314987
申请日：2005-12-21
CPC分类号： C12N9/58 , A23L13/424 , A23L33/18 , C12N9/48 , C12P7/06 , C12P7/10 , C12P19/02 , Y02E50/16 , Y02E50/17
摘要： The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.
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公开(公告)号：US08980598B2
公开(公告)日：2015-03-17
申请号：US11151919
申请日：2005-06-14
申请人： Gopal Chotani , Gang Duan , Craig E. Pilgrim , Jayarama K. Shetty
发明人： Gopal Chotani , Gang Duan , Craig E. Pilgrim , Jayarama K. Shetty
摘要： A dry solids staging fermentation process for producing an end-product, such as ethanol is disclosed said process including an initial fermentation step including combining a first fermentable substrate with one or more starch hydrolyzing enzymes and fermenting organisms in a fermentation vessel and a loading step which includes adding a second fermentable substrate to the fermentation vessel wherein the percent dry solids (% DS) of the fermentation broth increases over time.
摘要翻译： 公开了用于生产最终产物如乙醇的干固体分段发酵方法，所述方法包括初始发酵步骤，包括在发酵容器中组合第一可发酵底物与一种或多种淀粉水解酶和发酵生物体，以及装载步骤 包括向发酵容器中加入第二可发酵底物，其中发酵液的干固体百分比（％DS）随时间增加。
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公开(公告)号：US08916369B2
公开(公告)日：2014-12-23
申请号：US12531970
申请日：2008-03-12
CPC分类号： C12N9/242
摘要： A maltogenic a-amylase from Trichoderma reesei (TrAA) and variants thereof are useful in the production of high-maltose syrups from liquefied starch. Particularly high maltose concentrations are achieved when TrAA is used in the presence of a pullulanase. Expression hosts and encoding nucleic acids useful for producing TrAA and its variants also are provided.
摘要翻译： 来自里氏木霉（TrAA）的麦芽糖α-淀粉酶及其变体可用于从液化淀粉生产高麦芽糖浆。 当在存在支链淀粉酶的情况下使用TrAA时，可实现特别高的麦芽糖浓度。 还提供了用于产生TrAA及其变体的表达宿主和编码核酸。
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7.发明申请Grain Compositions Containing Pre-Biotic Isomal To-Oligosaccharides And Methods Of Making And Using Same 有权含有预生物异构异构寡糖的谷物组合物及其制备和使用方法公开(公告)号：US20120135108A1
公开(公告)日：2012-05-31
申请号：US13172744
申请日：2011-06-29
申请人： Gang Duan , Feng Li , Jayarama K. Shetty , Julius Vadakoot , Sridevi Vadakoot
发明人： Gang Duan , Feng Li , Jayarama K. Shetty , Julius Vadakoot , Sridevi Vadakoot
摘要： Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting said maltose with a transglucosidic enzyme, wherein said steps (a) and step (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.
摘要翻译： 描述了生产含有异麦芽低聚糖的底物，块茎和谷物组合物的方法。 所述方法包括（a）使含有未凝胶化淀粉的底物，块茎或谷粒与麦芽糖酶和淀粉液化酶接触以产生麦芽糖; （b）使所述麦芽糖与转葡糖苷酶接触，其中所述步骤（a）和步骤（b）在低于淀粉糊化温度或淀粉糊化温度的温度下进行; 和（c）获得具有酶促产生的异麦芽低聚糖的底物，谷物或块茎组合物，其中寡糖衍生自谷物。 麦芽糖酶可以是外源的或内源的谷物。 接触步骤可以是顺序的或并发的。 本发明还描述了包含如上所述制备的谷物组合物的面粉，口服再水合溶液，啤酒助剂，食品，饲料，饮料添加剂。
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8.发明授权Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same 有权含有预生物异麦芽低聚糖的颗粒组合物及其制备和使用方法公开(公告)号：US07993689B2
公开(公告)日：2011-08-09
申请号：US12616595
申请日：2009-11-11
申请人： Gang Duan , Fengi Li , Jayarama K. Shetty , Julius Vadakoot , Sridevi Vadakoot, legal representative
发明人： Gang Duan , Fengi Li , Jayarama K. Shetty , Julius Vadakoot
摘要： Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting the maltose with a transglucosidic enzyme, wherein the steps (a) and (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.
摘要翻译： 描述了生产含有异麦芽低聚糖的底物，块茎和谷物组合物的方法。 所述方法包括（a）使含有未凝胶化淀粉的底物，块茎或谷粒与麦芽糖酶和淀粉液化酶接触以产生麦芽糖; （b）使麦芽糖与转葡糖苷酶接触，其中步骤（a）和（b）在低于淀粉糊化温度或淀粉糊化温度的温度下进行; 和（c）获得具有酶促产生的异麦芽低聚糖的底物，谷物或块茎组合物，其中寡糖衍生自谷物。 麦芽糖酶可以是外源的或内源的谷物。 接触步骤可以是顺序的或并发的。 本发明还描述了包含如上所述制备的谷物组合物的面粉，口服再水合溶液，啤酒助剂，食品，饲料，饮料添加剂。
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公开(公告)号：US20130203140A1
公开(公告)日：2013-08-08
申请号：US13566923
申请日：2012-08-03
申请人： Martien H. BERGSMA , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
发明人： Martien H. BERGSMA , Anthony R. Calabria , Gopal K. Chotani , William A. Cuevas , Gang Duan , Sung Ho Lee , Ying Qian , Vivek Sharma , Jayarama K. Shetty , Bruce A. Strohm , Paula Johanna Maria Teunissen , Hongxian Xu
IPC分类号： C12P5/00
CPC分类号： C12P5/007 , C12N9/1085 , C12N9/2428 , C12Y205/0101 , C12Y302/01003
摘要： Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.
摘要翻译： 本公开的实施方案涉及通过糖化和/或发酵从淀粉底物生产类异戊二烯前体分子和/或类异戊二烯的方法。 糖化在pH为5.0至8.0的范围内由葡糖淀粉酶有效地催化。 在6.0或更高的pH下，葡糖淀粉酶相对于其最大活性具有至少50％的活性。 糖化和发酵可以作为同时糖化和发酵（SSF）方法进行。
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公开(公告)号：US20130142903A1
公开(公告)日：2013-06-06
申请号：US13817132
申请日：2011-08-19
IPC分类号： A23J3/34
CPC分类号： A23J3/346 , A23G1/54 , A23G3/54 , A23J1/125 , A23J3/14 , A23K20/147 , A23L33/185 , A23V2002/00 , C12Y302/01001 , C12Y302/01003 , A23V2250/548
摘要： Rice protein concentrates prepared at low temperature exhibit improved functionality and beneficial physiological benefits, including lowered cholesterol and enhanced lactic acid dehydrogenase activity, without an increase in blood urea nitrogen. The rice protein concentration could be made into a wet dough with comparatively less water than a soy protein concentrate. Use of the rice protein concentrate thus improved processing steps in the formulation of a food article containing the concentrate. The food product advantageously shows an extending shelf life and improved palatable texture.
摘要翻译： 在低温下制备的大米蛋白浓缩物显示改善的功能和有益的生理益处，包括降低的胆固醇和提高的乳酸脱氢酶活性，而不增加血液尿素氮。 大米蛋白质浓度可以制成湿面团，水分比大豆蛋白浓缩物少。 大米蛋白浓缩物的使用从而改善了含有浓缩物的食品制剂的加工步骤。 食品有利地显示出延长的保质期和改善的可口的质感。
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