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    In vivo biosensor apparatus and method of use
    5.
    发明授权
    In vivo biosensor apparatus and method of use 失效
    体内生物传感器装置及其使用方法

    公开(公告)号：US06673596B1

    公开(公告)日：2004-01-06

    申请号：US09454071

    申请日：1999-12-02

    申请人： Gary S. Sayler ,  Michael L. Simpson ,  Bruce M. Applegate ,  Steven A. Ripp

    发明人： Gary S. Sayler ,  Michael L. Simpson ,  Bruce M. Applegate ,  Steven A. Ripp

    IPC分类号： C12M300

    CPC分类号： C12Q1/02 ,  A61B5/0031 ,  C12Q1/66 ,  G01N21/763 ,  G01N33/74 ,  G01N33/84 ,  Y10S435/808 ,  Y10S435/849 ,  Y10S435/879 ,  Y10S435/909 ,  Y10S436/805

    摘要： Disclosed are bioluminescent bioreporter integrated circuit devices that detect selected analytes in fluids when implanted in the body of an animal. The device comprises a bioreporter that has been genetically engineered to contain a nucleic acid segment that comprises a cis-activating response element that is responsive to the selected substance operably linked to a gene encoding a bioluminescent reporter polypeptide. In preferred embodiments, the target analyte is glucose, glucagons, or insulin. Exposure of the bioreporter to the target substance causes the response element to up-regulate the nucleic acid sequence encoding the reporter polypeptide to produce a luminescent response that is detected and quantitated. In illustrative embodiments, the bioreporter device is encapsulated on an integrated circuit that is capable of detecting the emitted light, processing the resultant signal, and then remotely reporting the results. Also disclosed are controlled drug delivery systems capable of being directly or indirectly controlled by the detection device that provide drugs such as insulin to the animal in reponse to the amount of target analyte present in the body fluids.

    摘要翻译： 公开了生物发光生物循环器集成电路器件，其在植入动物身体时检测流体中的选定分析物。 该装置包括已被遗传工程化以包含核酸片段的生物携带者，所述核酸片段包含顺序激活响应元件，所述顺式活化响应元件可操作地连接到编码生物发光报告多肽的基因的选定物质。 在优选的实施方案中，目标分析物是葡萄糖，胰高血糖素或胰岛素。 将生物携带者暴露于目标物质导致响应元件上调编码报道多肽的核酸序列以产生检测和定量的发光反应。 在示例性实施例中，生物传送器装置封装在能够检测发射光，处理所得信号，然后远程报告结果的集成电路上。 还公开了能够由检测装置直接或间接控制的受控药物递送系统，所述检测装置响应于体液中存在的目标分析物的量，向动物提供诸如胰岛素的药物。

    Methods for cell-based combinatorial logic
    6.
    发明授权
    Methods for cell-based combinatorial logic 失效
    基于单元的组合逻辑的方法

    公开(公告)号：US07020560B2

    公开(公告)日：2006-03-28

    申请号：US09949015

    申请日：2001-09-06

    申请人： Gary S. Sayler ,  James T. Fleming ,  Bruce Applegate ,  Michael L. Simpson

    发明人： Gary S. Sayler ,  James T. Fleming ,  Bruce Applegate ,  Michael L. Simpson

    IPC分类号： G01N21/00 ,  G01N27/00 ,  G01N33/48

    CPC分类号： C12Q1/6897 ,  B82Y10/00 ,  G01N33/5005 ,  G01N33/5438 ,  G06N3/002

    摘要： A method for cell-based combinatorial logic includes the steps of providing at least one genetically engineered cell, the genetically engineered cell having at least one transcriptional unit. The transcriptional unit includes a gene and a promoter, wherein application of a stimulus to the promoter results in the expression of a gene product. An energetic or chemical stimulus is applied to activate the promoter, wherein the detection of an output signal corresponds to the presence of a gene product. The cell can include a plurality of transcriptional units configured to form logic gates. The logic gates of a plurality of cells can be operably interconnected by release of output signals, such as chemical stimuli.

    摘要翻译： 一种用于基于小区的组合逻辑的方法包括以下步骤：提供至少一个经遗传工程化的细胞，所述基因工程细胞具有至少一个转录单位。 转录单位包括基因和启动子，其中向启动子施加刺激物导致基因产物的表达。 施加能量或化学刺激以激活启动子，其中输出信号的检测对应于基因产物的存在。 该小区可以包括被配置成形成逻辑门的多个转录单元。 多个单元的逻辑门可以通过释放诸如化学刺激的输出信号来可操作地互连。