公开(公告)号：US07615009B2

公开(公告)日：2009-11-10

申请号：US11678038

申请日：2007-02-22

申请人： Glen P. Koste ,  Samhita Dasgupta ,  Matthew Christian Nielsen ,  Min-Yi Shih ,  Robert John Filkins ,  Todd Ryan Tolliver ,  Bruno Hans Haider

发明人： Glen P. Koste ,  Samhita Dasgupta ,  Matthew Christian Nielsen ,  Min-Yi Shih ,  Robert John Filkins ,  Todd Ryan Tolliver ,  Bruno Hans Haider

IPC分类号： A61B8/00

CPC分类号： A61B8/14 ,  A61B8/06 ,  A61B8/08 ,  A61B8/13 ,  A61B8/488 ,  A61B8/546 ,  G01S15/8968 ,  H04B10/505 ,  H04B11/00 ,  Y02D70/46 ,  Y02D70/48

摘要： A system and method for optical transmission of ultrasound data signals from a probe to an image processing system is provided. By using a silicon-based optical modulator to encode ultrasound data signals onto an optical signal, an optical transmission link between the ultrasound probe and the image processing system can achieve a high signal to noise ratio with a lower power input.

摘要翻译： 提供了一种用于从探针到图像处理系统的光学传输超声数据信号的系统和方法。 通过使用硅基光学调制器将超声波数据信号编码到光信号上，超声波探头与图像处理系统之间的光传输链路可以通过较低的功率输入实现较高的信噪比。

公开(公告)号：US07367945B2

公开(公告)日：2008-05-06

申请号：US10812243

申请日：2004-03-29

申请人： Samhita Dasgupta ,  Matthew Christian Nielsen ,  Min-Yi Shih ,  Robert John Filkins ,  Todd Ryan Tolliver ,  Bruno Hans Haider

发明人： Samhita Dasgupta ,  Matthew Christian Nielsen ,  Min-Yi Shih ,  Robert John Filkins ,  Todd Ryan Tolliver ,  Bruno Hans Haider

IPC分类号： A61B8/00

CPC分类号： A61B8/14 ,  A61B8/06 ,  A61B8/08 ,  A61B8/13 ,  A61B8/488 ,  A61B8/546 ,  G01S15/8968 ,  H04B10/505 ,  H04B11/00 ,  Y02D70/46 ,  Y02D70/48

摘要： An ultrasound system includes an ultrasound probe configured for sensing and transmitting ultrasound signals. The ultrasound system further includes an optical conduit configured for coupling a light source and an optical detector in an optical path. The optical conduit includes electro-optic modulators configured for modulating optical signals on the optical conduit with at least one of the electrical signals configured to generate corresponding optically modulated analog signals on the optical conduit. In one example, the electro-optic modulators comprise electro-optic polymer modulators.

摘要翻译： 超声波系统包括被配置用于感测和发送超声信号的超声波探头。 超声系统还包括配置用于将光源和光学检测器耦合在光路中的光导管。 光导管包括被配置用于调制光导管上的光信号的电光调制器，其中至少一个电信号被配置为在光导管上产生对应的光调制模拟信号。 在一个实例中，电光调制器包括电光聚合物调制器。

公开(公告)号：US07327022B2

公开(公告)日：2008-02-05

申请号：US10248237

申请日：2002-12-30

申请人： Glenn Scott Claydon ,  Matthew Christian Nielsen ,  Samhita Dasgupta ,  Robert John Filkins ,  Glenn Alan Forman

发明人： Glenn Scott Claydon ,  Matthew Christian Nielsen ,  Samhita Dasgupta ,  Robert John Filkins ,  Glenn Alan Forman

IPC分类号： H01L23/48 ,  H01L23/52 ,  H01L29/40 ,  H01L23/29 ,  H01L23/02

CPC分类号： G02B6/4206 ,  G02B6/4214 ,  G02B6/43 ,  H01L24/19 ,  H01L24/20 ,  H01L2224/73267 ,  H01L2224/9222 ,  H01L2924/12041 ,  H01L2924/12042 ,  H01L2924/12043 ,  H01L2924/14 ,  H01L2924/00

摘要： A novel micro optical system as a platform technology for electrical and optical interconnections, thermal and mechanical assembly and integration of electronic, optoelectronic, passive and active components. This platform provides optical coupling and chip-to-chip interconnection by microwave electrical, optical guided and unguided waves, and power or bias electrical contacts or interfaces by a novel chip in flexible circuit, rigid or inflexible embodiments.

摘要翻译： 一种新颖的微光学系统作为电气和光学互连的平台技术，电子，光电子，被动和有源组件的热和机械组装和集成。 该平台通过柔性电路，刚性或不灵活的实施例中的新型芯片提供了通过微波电，光引导和非导向波以及功率或偏置电触点或接口的光耦合和芯片到芯片互连。

公开(公告)号：US07310408B2

公开(公告)日：2007-12-18

申请号：US11096488

申请日：2005-03-31

申请人： Robert John Filkins ,  John Scott Price ,  Brian Lee Lawrence ,  Matthew Christian Nielsen ,  Joseph John Manak ,  Bruce Matthew Dunham

发明人： Robert John Filkins ,  John Scott Price ,  Brian Lee Lawrence ,  Matthew Christian Nielsen ,  Joseph John Manak ,  Bruce Matthew Dunham

IPC分类号： H05G2/00

CPC分类号： H05G2/00

摘要： A system for generating a tunable X-ray pulse comprises a first electron beam source configured to direct a first electron pulse of predetermined energy and pulse length towards a first interaction zone, a laser beam source configured to direct a first photon pulse of predetermined energy and pulse length towards the first interaction zone to interact with the first electron pulse. The first interaction produces a substantially monochromatic second photon pulse of higher photon energy directed towards a second interaction zone, and a second electron beam source configured to direct a second electron pulse of predetermined energy and pulse length towards the second interaction zone so that the second interaction produces an X-ray pulse of predetermined energy and pulse length in a cascaded inverse Compton scattering (ICS) configuration.

摘要翻译： 一种用于产生可调谐X射线脉冲的系统包括：第一电子束源，被配置为将预定能量和脉冲长度的第一电子脉冲引导到第一相互作用区域，激光束源被配置为引导预定能量的第一光子脉冲;以及 脉冲长度朝向第一相互作用区域与第一电子脉冲相互作用。 所述第一相互作用产生指向第二相互作用区的较高光子能量的基本上单色的第二光子脉冲，以及被配置成将预定能量和脉冲长度的第二电子脉冲引向所述第二相互作用区域的第二电子束源， 在级联反康普顿散射（ICS）配置中产生预定能量和脉冲长度的X射线脉冲。

公开(公告)号：US08900529B2

公开(公告)日：2014-12-02

申请号：US13458092

申请日：2012-04-27

申请人： Kashan Ali Shaikh ,  Jessica Marie Godin ,  Alex David Corwin ,  Robert John Filkins

发明人： Kashan Ali Shaikh ,  Jessica Marie Godin ,  Alex David Corwin ,  Robert John Filkins

IPC分类号： B01L99/00

CPC分类号： B32B37/0076 ,  B01L3/5027 ,  B01L3/502707 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0655 ,  B32B37/182 ,  B32B38/0008 ,  B32B2535/00 ,  Y10T156/10 ,  Y10T156/1056 ,  Y10T156/108

摘要： A microfluidic flow cell subassembly, which may be assembled into a flow cell having fluidic connections outside of the main substrate, is described for encapsulating a sample to allow for subsequent controlled delivery of reagents to the sample, such as multiplexed in situ biomarker staining and analysis. Methods for fabricating the subassembly and assembled flow cell are also provided. The method includes the steps of adhering a gasket layer to a substrate layer, wherein the gasket layer may contain enclosed features and adhering an adherent layer to the gasket layer. The subassembly may be sealed against a solid support to form a flow cell.

摘要翻译： 描述了可以组装成具有在主底物外部的流体连接的流动池的微流体流动池子组件，用于封装样品以允许随后控制的试剂递送到样品中，例如原位复合生物标记染色和分析 。 还提供了用于制造子组件和组装流动池的方法。 该方法包括以下步骤：将衬垫层粘附到衬底层，其中衬垫层可以包含封闭特征并将粘附层粘附到衬垫层。 子组件可以密封在固体支撑件上以形成流动池。

公开(公告)号：US20120231967A1

公开(公告)日：2012-09-13

申请号：US13457531

申请日：2012-04-27

申请人： Anup Sood ,  Michael Christopher Montalto ,  Michael Gerdes ,  Maximilian Lewis Seel ,  Robert John Filkins

发明人： Anup Sood ,  Michael Christopher Montalto ,  Michael Gerdes ,  Maximilian Lewis Seel ,  Robert John Filkins

IPC分类号： C40B30/04

CPC分类号： G01N33/53 ,  C12Q1/68 ,  G01N33/542 ,  G01N33/582 ,  Y10T436/143333

摘要： Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.

摘要翻译： 提供了用于检测生物样品中多个靶的方法。 所述方法包括使样品与第一探针接触; 将第一探针物理地绑定到第一靶; 观察来自第一探针的第一信号; 施用化学试剂来修饰第一信号; 使样品与第二探针接触; 将第二探针物理地绑定到第二靶上; 并观察来自第二探测器的第二信号。 本文公开的方法还提供绑定，观察，信号修改的多次迭代，用于导出关于单个样本中的多个目标的信息。 还提供了相关的套件和设备。

公开(公告)号：US20120135458A1

公开(公告)日：2012-05-31

申请号：US12957203

申请日：2010-11-30

申请人： Alex David Corwin ,  Robert John Filkins ,  Jun Xie ,  Christopher James Sevinsky ,  Kashan Ali Shaikh

发明人： Alex David Corwin ,  Robert John Filkins ,  Jun Xie ,  Christopher James Sevinsky ,  Kashan Ali Shaikh

IPC分类号： G01N1/30 ,  C12M1/36

CPC分类号： G01N1/312 ,  G01N2001/368

摘要： A closed loop automated method for staining of a biological sample is provided. The method comprises providing a biological sample, staining at least a portion of the biological sample by flowing in a reagent, monitoring one or more optical characteristics of the biological sample, and calculating a figure of merit based on at least one of the optical characteristics. An automated device for iterative staining of a biological sample is also provided.

摘要翻译： 提供了一种用于染色生物样品的闭环自动化方法。 该方法包括提供生物样品，通过在试剂中流动来染色生物样品的至少一部分，监测生物样品的一种或多种光学特性，以及基于至少一种光学特性来计算品质因数。 还提供了用于生物样品的迭代染色的自动化装置。

公开(公告)号：US20120135449A1

公开(公告)日：2012-05-31

申请号：US13365525

申请日：2012-02-03

申请人： Jun Xie ,  Robert John Filkins ,  Fiona Mary Ginty ,  Michael Christopher Montalto ,  Anup Sood ,  Jeffrey Bernard Fortin ,  Wei-Cheng Tian ,  Michael John Gerdes

发明人： Jun Xie ,  Robert John Filkins ,  Fiona Mary Ginty ,  Michael Christopher Montalto ,  Anup Sood ,  Jeffrey Bernard Fortin ,  Wei-Cheng Tian ,  Michael John Gerdes

IPC分类号： G01N21/75 ,  C12M1/34

CPC分类号： G01N1/312

摘要： Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent, and releasing the sample from the flow cell wherein the integrity of the sample is intact. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell.

摘要翻译： 提供了促进成像应用中生物样品的迭代染色的自动化方法和装置。 所述方法包括以下步骤：提供含有生物样品的小体积流动池，向生物样品施加染色，将至少两种前体试剂结合以形成活化的脱色剂，并且其中所述活化脱色剂分解速率大于或等于 以脱色反应速率使脱色剂以大于活化脱色剂的分解速率的流速流过生物样品，并将样品从流动池中释放出来，其中样品的完整性是完整的。 染色，组合和流动的过程可以迭代地重复。 本文还公开了用于重复染色生物样品的装置，其包括与预混合器流体连通的流动池，其中预混合器的体积容量小于流动池的体积容量的约五倍。

公开(公告)号：US20110075914A1

公开(公告)日：2011-03-31

申请号：US12571158

申请日：2009-09-30

申请人： Robert John Filkins ,  Kevin Kenny ,  David Henderson ,  Jens Rittscher

发明人： Robert John Filkins ,  Kevin Kenny ,  David Henderson ,  Jens Rittscher

IPC分类号： G06K9/00

CPC分类号： G06K9/00127 ,  G06K9/036 ,  G06T7/0002 ,  G06T7/41 ,  G06T2200/32 ,  G06T2207/10056 ,  G06T2207/30024

摘要： The present disclosure concerns a method and system for assessing image quality of sub-images used to form a composite image using an algorithm developed by applying statistical correlation techniques to historical data on features of sub-images which were manually evaluated by experts and using those assessment to make decisions about the further processing of the sub-images which are being assessed. The method and system find particular value in the processing of sub-images generated while there is relative motion between the specimen under examination and the objective lens of a microscope such as when the microscope stage follows a planned traverse in the focal plane and a digital image is created at intervals correlated to the motion of the stage so that a region of interest in the specimen under examination is covered by the sub-images. The further processing decisions include manually examining the sub-images assessed to have unacceptable image quality, reimaging the entire region of interest and recreating specific sub-images. The method and system may also involve overlaying portions of a composite image with an indication that they were drawn from sub-images of unacceptable image quality.

摘要翻译： 本公开涉及一种用于评估用于形成合成图像的子图像的图像质量的方法和系统，该方法和系统使用通过将统计相关技术应用于由专家手动评估的子图像的特征的历史数据而开发的算法，并使用这些评估 做出关于正在评估的子图像的进一步处理的决定。 该方法和系统在检查中的样本与显微镜的物镜之间产生的子图像的处理中发现特定的值，例如当显微镜载物台在焦平面上进行计划的横越时，以及数字图像 以与舞台的运动相关的间隔创建，使得被检查的样本中的感兴趣区域被子图像覆盖。 进一步的处理决定包括手动检查评估为具有不可接受的图像质量的子图像，重新映像整个感兴趣区域并重新创建特定子图像。 该方法和系统还可以包括将合成图像的部分重叠，并显示它们是从不可接受的图像质量的子图像中提取的。

公开(公告)号：US20100301230A1

公开(公告)日：2010-12-02

申请号：US12474306

申请日：2009-05-29

申请人： Robert John Filkins ,  Robert William Tait ,  Kevin Bernard Kenny ,  Christina Chirappuram Royce

发明人： Robert John Filkins ,  Robert William Tait ,  Kevin Bernard Kenny ,  Christina Chirappuram Royce

IPC分类号： G01N21/64

CPC分类号： G01N21/6452 ,  G01N21/6458 ,  G01N21/6486 ,  G02B21/16 ,  G02B2207/113

摘要： The invention provides method for locating one or more substantially circular-shaped tissue sample positioned on a solid support. The method involves the steps of transmitting light of a preselected wavelength onto a tissue sample, wherein the light induces the tissue sample to autofluoresce, identifying the center location of the tissue sample using the autofluoresced light, correlating the coordinates of the center location of the tissue sample on the solid support using an x, y-coordinate system, and mapping the coordinates of the tissue sample on the solid support to differentiate tissue sample containing regions from blank regions on the solid support. In a second aspect, the invention provides an apparatus for locating one or more substantially circular-shaped tissue sample positioned on a solid support.

摘要翻译： 本发明提供了一种用于定位位于固体支持体上的一个或多个基本圆形的组织样本的方法。 该方法包括以下步骤：将预选波长的光透射到组织样本上，其中光诱导组织样本自发荧光，使用自发荧光识别组织样本的中心位置，将组织的中心位置的坐标相关联 使用x，y坐标系统在固体支持物上进行样品测绘，并将组织样本的坐标映射到固体支持物上，以将含有区域的组织样本与固体支持物上的空白区域区分开。 在第二方面，本发明提供了一种用于定位位于固体支撑体上的一个或多个基本圆形的组织样本的装置。