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公开(公告)号：US20110318829A1
公开(公告)日：2011-12-29
申请号：US13229087
申请日：2011-09-09
申请人： Go TAZAKI , Tomoko Kosaka , Motohiro Fukuda
发明人： Go TAZAKI , Tomoko Kosaka , Motohiro Fukuda
摘要： To provide a cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space. A cell culture chamber (10) according to the present invention is a cell culture chamber (10) including a plurality of microchambers (11) formed on a surface thereof, characterized in that convex portions (side walls 12) that partition the microchambers (11) adjacent to each other are formed to prevent cells from being adhered to upper surfaces of the convex portions. Consequently, cells can be cultured exclusively within the microchambers (11), and an intercellular network can be constructed effectively.
摘要翻译： 提供能够有效地构建培养空间中的细胞间网络的细胞培养室和细胞培养方法。 根据本发明的细胞培养室（10）是包括形成在其表面上的多个微室（11）的细胞培养室（10），其特征在于，将微型室（11）分隔开的凸部（侧壁12） ）形成为防止细胞粘附到凸部的上表面。 因此，可以在微室（11）内专门培养细胞，并且可以有效地构建细胞间网络。
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2.发明授权公开(公告)号：US09428721B2
公开(公告)日：2016-08-30
申请号：US12866581
申请日：2009-02-05
申请人： Hideki Taniguchi , Yun-Wen Zheng , Go Tazaki , Tomoko Kosaka , Hitoshi Tsuruta , Motohiro Fukuda
发明人： Hideki Taniguchi , Yun-Wen Zheng , Go Tazaki , Tomoko Kosaka , Hitoshi Tsuruta , Motohiro Fukuda
CPC分类号： C12M23/22 , C12M23/12 , G01N33/5073
摘要： Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.
摘要翻译： 本发明提供能够长期维持体内功能的细胞培养方法，并且可以使用所需的最少细胞数进行培养。 细胞培养方法包括在分层微空间中以分层状态培养未分化细胞，获得分化细胞。 当筛选药剂时，优选能够分化为肝细胞，肠上皮细胞，神经细胞，心肌细胞和血管内皮细胞的未分化细胞。 特别是在人体的药代动力学等的预测中，优选人细胞。
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公开(公告)号：US20100190253A1
公开(公告)日：2010-07-29
申请号：US12665474
申请日：2008-06-16
申请人： Go Tazaki , Tomoko Kosaka , Motohiro Fukuda
发明人： Go Tazaki , Tomoko Kosaka , Motohiro Fukuda
摘要： To provide a cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space. A cell culture chamber (10) according to the present invention is a cell culture chamber (10) including a plurality of microchambers (11) formed on a surface thereof, characterized in that convex portions (side walls 12) that partition the microchambers (11) adjacent to each other are formed to prevent cells from being adhered to upper surfaces of the convex portions. Consequently, cells can be cultured exclusively within the microchambers (11), and an intercellular network can be constructed effectively.
摘要翻译： 提供能够有效地构建培养空间中的细胞间网络的细胞培养室和细胞培养方法。 根据本发明的细胞培养室（10）是包括形成在其表面上的多个微室（11）的细胞培养室（10），其特征在于，将微型室（11）分隔开的凸部（侧壁12） ）形成为防止细胞粘附到凸部的上表面。 因此，可以在微室（11）内专门培养细胞，并且可以有效地构建细胞间网络。
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公开(公告)号：US08415157B2
公开(公告)日：2013-04-09
申请号：US13229087
申请日：2011-09-09
申请人： Go Tazaki , Tomoko Kosaka , Motohiro Fukuda
发明人： Go Tazaki , Tomoko Kosaka , Motohiro Fukuda
摘要： A cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space are provided. A cell culture chamber (10)according to the present invention is a cell culture chamber (10)including a plurality of microchambers (11) formed on a surface thereof, characterized in that convex portions (side walls 12) that partition the microchambers (11) adjacent to each other are formed to prevent cells from being adhered to upper surfaces of the convex portions. Consequently, cells can be cultured exclusively within the microchambers (11), and an intercellular network can be constructed effectively.
摘要翻译： 提供了能够有效地构建培养空间中的细胞间网络的细胞培养室和细胞培养方法。 根据本发明的细胞培养室（10）是包括形成在其表面上的多个微室（11）的细胞培养室（10），其特征在于，将微型室（11）分隔开的凸部（侧壁12） ）形成为防止细胞粘附到凸部的上表面。 因此，可以在微室（11）内专门培养细胞，并且可以有效地构建细胞间网络。
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公开(公告)号：US20110045500A1
公开(公告)日：2011-02-24
申请号：US12866581
申请日：2009-02-05
申请人： Hideki Taniguchi , Yun-Wen Zheng , Go Tazaki , Tomoko Kosaka , Hitoshi Tsuruta , Motohiro Fukuda
发明人： Hideki Taniguchi , Yun-Wen Zheng , Go Tazaki , Tomoko Kosaka , Hitoshi Tsuruta , Motohiro Fukuda
CPC分类号： C12M23/22 , C12M23/12 , G01N33/5073
摘要： Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.
摘要翻译： 本发明提供能够长期维持体内功能的细胞培养方法，并且可以使用所需的最少细胞数进行培养。 细胞培养方法包括在分层微空间中以分层状态培养未分化细胞，获得分化细胞。 当筛选药剂时，优选能够分化为肝细胞，肠上皮细胞，神经细胞，心肌细胞和血管内皮细胞的未分化细胞。 特别是在人体的药代动力学等的预测中，优选人细胞。
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公开(公告)号：US20110207215A1
公开(公告)日：2011-08-25
申请号：US13125871
申请日：2009-10-23
申请人： Yoko Itchoda , Go Tazaki , Motohiro Fukuda , Hitoshi Tsuruta , Hideki Taniguchi
发明人： Yoko Itchoda , Go Tazaki , Motohiro Fukuda , Hitoshi Tsuruta , Hideki Taniguchi
IPC分类号： C12N5/071 , C12N5/0735 , C12N5/0793 , C12N5/077
摘要： To provide a cell storage method to store living cells while maintaining the functions of the living cells. An aspect of the cell storage method is a method to store living cells (40) by using a cell culture chamber (20) including a plurality of the microchambers (11), the method including: culturing the living cells by being adhered to the surfaces of a plurality of micro spaces; and after the culturing, pouring the culture medium (50) into the cell culture chamber (20) so as to cover the plurality of microchambers (11), and storing the living cells. The living cells are adhered to a cell culture chamber of an appropriate size and cultured to form a three-dimensional structure. This enables maintaining the three-dimensional structure of the living cells and storage of the living cells while maintaining the functions thereof.
摘要翻译： 提供细胞储存方法来储存活细胞同时保持活细胞的功能。 细胞储存方法的一个方面是通过使用包括多个微室（11）的细胞培养室（20）来储存活细胞（40）的方法，所述方法包括：通过粘附到所述表面上培养活细胞 的多个微空间; 培养后，将培养基（50）注入到细胞培养室（20）中以覆盖多个微室（11），并储存活细胞。 将活细胞粘附到适当尺寸的细胞培养室并进行培养以形成三维结构。 这能够维持活细胞的三维结构并维持活体的功能。
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公开(公告)号：US09290735B2
公开(公告)日：2016-03-22
申请号：US12866588
申请日：2009-02-05
申请人： Masahiro Sokabe , Kishio Furuya , Go Tazaki , Hitoshi Tsuruta , Motohiro Fukuda
发明人： Masahiro Sokabe , Kishio Furuya , Go Tazaki , Hitoshi Tsuruta , Motohiro Fukuda
CPC分类号： C12N5/0631 , C12M23/12 , C12M23/16 , C12M23/22 , C12N5/0062 , C12N2535/10
摘要： To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a hollow acinus-like structure can be formed. The minute partitioned space is particularly preferably a micro container in a cell culture container having a plurality of such micro containers on the surface.
摘要翻译： 提供能够通过抑制去分化来长时间维持体内功能的细胞培养方法。 根据本发明的细胞培养方法是在微小分隔空间中以多层状态培养细胞，并获得具有类似于体内功能的功能的组织结构。 当细胞是乳腺上皮细胞时，可以形成中空的类固醇样结构。 微细分隔空间特别优选为细胞培养容器中的微型容器，其具有表面上的多个这种微量容器。
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8.发明申请公开(公告)号：US20130071932A1
公开(公告)日：2013-03-21
申请号：US13636855
申请日：2011-03-23
申请人： Yoko Itchoda , Go Tazaki , Masaya Hosoda , Motohiro Fukuda , Hideki Taniguchi , Yun-Wen Zheng , Keisuke Sekine
发明人： Yoko Itchoda , Go Tazaki , Masaya Hosoda , Motohiro Fukuda , Hideki Taniguchi , Yun-Wen Zheng , Keisuke Sekine
IPC分类号： C12N5/071 , C12N5/095 , C12N5/076 , C12N5/0735
CPC分类号： C12N5/0606 , C12M23/12 , C12N2502/28 , C12N2506/02 , C12N2535/10
摘要： Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).
摘要翻译： 提供了通过使用其中形成有多个微室的细胞培养室，实现胚状体大小的控制并且可以在控制胚状体大小的状态下诱导分化的方法。 用于引起多能哺乳动物细胞分化的培养方法使用在培养表面上形成有多个微室（11）的细胞培养室（10）。 细胞培养室（10）具有由空间形成的培养面，其中微室（11）具有高度为10μm〜500μm，底面积为100μm2〜1mm2的空间结构。 引起多能哺乳动物细胞分化的培养方法包括通过使用细胞培养室（10）培养多能哺乳动物细胞以获得至少部分分化为内胚层谱系细胞的细胞群。
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9.发明申请MICROCHANNEL ARRAY AND METHOD FOR PRODUCING THE SAME, AND BLOOD MEASURING METHOD EMPLOYING IT 审中-公开MICROCHANNEL ARRAY AND METHOD FOR PRODUCTION THE SAME，和BLOOD MEASURING METHODWORKING IT公开(公告)号：US20090149345A1
公开(公告)日：2009-06-11
申请号：US11908047
申请日：2006-03-01
申请人： Taiji Nishi , Motohiro Fukuda , Go Tazaki , Seiichi Kanai , Takenori Kitani , Naoto Fukuhara
发明人： Taiji Nishi , Motohiro Fukuda , Go Tazaki , Seiichi Kanai , Takenori Kitani , Naoto Fukuhara
CPC分类号： G01N33/80 , B01L3/502707 , B01L3/502746 , B01L2300/0681 , B01L2300/0816 , G01N33/56972
摘要： A microchannel array, a method of manufacturing the same, and a blood test method. The microchannel array is formed by joining first and second substrates, each including a fluid inlet and outlet on their surfaces. An internal space structure connects the fluid inlet and outlet, and includes an upstream flow channel connected with the fluid inlet, a downstream flow channel connected with the fluid outlet with a gap therebetween, and a micro flow channel connecting the upstream and downstream flow channels. A minimum distance from a center of a sectional surface of the micro flow channel to a side wall of the micro flow channel is smaller than that of the upstream and downstream flow channels. Each surface of the first and second substrates includes grooves for creating the upstream and downstream flow channels, and the surface of the second substrate has grooves for creating the micro flow channel.
摘要翻译： 微通道阵列，其制造方法和血液检查方法。 微通道阵列通过接合第一和第二基底而形成，每个基底在其表面上包括流体入口和出口。 内部空间结构连接流体入口和出口，并且包括与流体入口连接的上游流动通道，与流体出口连接的下游流动通道，其间具有间隙，以及连接上游和下游流动通道的微流动通道。 从微流动通道的截面的中心到微流动通道的侧壁的最小距离小于上游和下游流动通道的最小距离。 第一和第二基板的每个表面包括用于产生上游和下游流动通道的槽，并且第二基板的表面具有用于产生微流动通道的凹槽。
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公开(公告)号：US10513685B2
公开(公告)日：2019-12-24
申请号：US13636855
申请日：2011-03-23
申请人： Yoko Itchoda , Go Tazaki , Masaya Hosoda , Motohiro Fukuda , Hideki Taniguchi , Yun-Wen Zheng , Keisuke Sekine
发明人： Yoko Itchoda , Go Tazaki , Masaya Hosoda , Motohiro Fukuda , Hideki Taniguchi , Yun-Wen Zheng , Keisuke Sekine
IPC分类号： C12N5/071 , C12N5/0735 , C12M1/32
摘要： Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).
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