公开(公告)号：US20090149345A1

公开(公告)日：2009-06-11

申请号：US11908047

申请日：2006-03-01

申请人： Taiji Nishi ,  Motohiro Fukuda ,  Go Tazaki ,  Seiichi Kanai ,  Takenori Kitani ,  Naoto Fukuhara

发明人： Taiji Nishi ,  Motohiro Fukuda ,  Go Tazaki ,  Seiichi Kanai ,  Takenori Kitani ,  Naoto Fukuhara

IPC分类号： C40B30/10 ,  C40B40/00 ,  C40B50/00

CPC分类号： G01N33/80 ,  B01L3/502707 ,  B01L3/502746 ,  B01L2300/0681 ,  B01L2300/0816 ,  G01N33/56972

摘要： A microchannel array, a method of manufacturing the same, and a blood test method. The microchannel array is formed by joining first and second substrates, each including a fluid inlet and outlet on their surfaces. An internal space structure connects the fluid inlet and outlet, and includes an upstream flow channel connected with the fluid inlet, a downstream flow channel connected with the fluid outlet with a gap therebetween, and a micro flow channel connecting the upstream and downstream flow channels. A minimum distance from a center of a sectional surface of the micro flow channel to a side wall of the micro flow channel is smaller than that of the upstream and downstream flow channels. Each surface of the first and second substrates includes grooves for creating the upstream and downstream flow channels, and the surface of the second substrate has grooves for creating the micro flow channel.

摘要翻译： 微通道阵列，其制造方法和血液检查方法。 微通道阵列通过接合第一和第二基底而形成，每个基底在其表面上包括流体入口和出口。 内部空间结构连接流体入口和出口，并且包括与流体入口连接的上游流动通道，与流体出口连接的下游流动通道，其间具有间隙，以及连接上游和下游流动通道的微流动通道。 从微流动通道的截面的中心到微流动通道的侧壁的最小距离小于上游和下游流动通道的最小距离。 第一和第二基板的每个表面包括用于产生上游和下游流动通道的槽，并且第二基板的表面具有用于产生微流动通道的凹槽。

公开(公告)号：US20090170190A1

公开(公告)日：2009-07-02

申请号：US12280607

申请日：2007-02-19

申请人： Taiji Nishi ,  Go Tazaki ,  Motohiro Fukuda ,  Michio Yazawa ,  Masayuki Takahashi

发明人： Taiji Nishi ,  Go Tazaki ,  Motohiro Fukuda ,  Michio Yazawa ,  Masayuki Takahashi

IPC分类号： C12M1/00 ,  B05D1/32

CPC分类号： C12M23/12 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2300/0893 ,  C12M25/00

摘要： An object of the present invention is to provide a cell culture chamber with which the survival rate of cells to be cultured can be increased, and a method of producing the same. A cell culture chamber according to the present invention includes a mass of projections formed of a plurality of microprojections 1 formed on a surface on which cells are cultured. The width or diameter of each of the microprojections 1 is in a range of 20 nm to 3 μm, and the aspect ratio of each of the microprojections is in a range of 0.2 to 3.0. Thus, there can be provided a cell culture chamber most suitable for the adhesion and differentiation/proliferation of cells to be cultured.

摘要翻译： 本发明的目的是提供一种能够提高培养细胞存活率的细胞培养室及其制造方法。 根据本发明的细胞培养室包括由在其上培养细胞的表面上形成的多个微突出物1形成的突起体。 每个微喷射体1的宽度或直径在20nm至3μm的范围内，并且每个微喷射体的纵横比在0.2至3.0的范围内。 因此，可以提供最适合于待培养细胞的粘附和分化/增殖的细胞培养室。

公开(公告)号：US20090075363A1

公开(公告)日：2009-03-19

申请号：US12298803

申请日：2007-04-25

申请人： Yuji Morimoto ,  Taiji Nishi ,  Taisuke Kamada ,  Go Tazaki ,  Motohiro Fukuda

发明人： Yuji Morimoto ,  Taiji Nishi ,  Taisuke Kamada ,  Go Tazaki ,  Motohiro Fukuda

IPC分类号： C12M3/00 ,  B05D5/10

CPC分类号： C12M23/12 ,  C12M23/16

摘要： To provide a cell culture chamber capable of reproducing a cell function in vivo, and a method of producing the same. The cell culture chamber according to the present invention has a concave-convex pattern formed on the surface on which cells are cultured. A concave portion of the concave-convex pattern includes cell culture portions and micro flow channels communicating with the cell culture portions. The bottom surface of each of the cell culture portions has a width 1.0 to 20 times an equivalent diameter of each of the cultured cells. A cell adhesion-inducing substance is formed only on the bottom surface and the side walls of the concave portion.

摘要翻译： 提供能够在体内再现细胞功能的细胞培养室及其制造方法。 根据本发明的细胞培养室具有形成在其上培养细胞的表面上的凹凸图案。 凹凸图案的凹部包括与细胞培养部连通的细胞培养部和微流路。 每个细胞培养部分的底表面具有每个培养细胞的当量直径的1.0至20倍的宽度。 细胞粘附诱导物质仅形成在凹部的底面和侧壁上。

公开(公告)号：US08652833B2

公开(公告)日：2014-02-18

申请号：US12298803

申请日：2007-04-25

申请人： Yuji Morimoto ,  Taiji Nishi ,  Taisuke Kamada ,  Go Tazaki ,  Motohiro Fukuda

发明人： Yuji Morimoto ,  Taiji Nishi ,  Taisuke Kamada ,  Go Tazaki ,  Motohiro Fukuda

IPC分类号： C12M3/00 ,  B05D5/10 ,  C40B40/00 ,  C04B40/02

CPC分类号： C12M23/12 ,  C12M23/16

摘要： To provide a cell culture chamber capable of reproducing a cell function in vivo, and a method of producing the same. The cell culture chamber according to the present invention has a concave-convex pattern formed on the surface on which cells are cultured. A concave portion of the concave-convex pattern includes cell culture portions and micro flow channels communicating with the cell culture portions. The bottom surface of each of the cell culture portions has a width 1.0 to 20 times an equivalent diameter of each of the cultured cells. A cell adhesion-inducing substance is formed only on the bottom surface and the side walls of the concave portion.

摘要翻译： 提供能够在体内再现细胞功能的细胞培养室及其制造方法。 根据本发明的细胞培养室具有形成在其上培养细胞的表面上的凹凸图案。 凹凸图案的凹部包括与细胞培养部连通的细胞培养部和微流路。 每个细胞培养部分的底表面具有每个培养细胞的当量直径的1.0至20倍的宽度。 细胞粘附诱导物质仅形成在凹部的底面和侧壁上。

公开(公告)号：US20090075366A1

公开(公告)日：2009-03-19

申请号：US11914762

申请日：2006-05-11

申请人： Go Tazaki ,  Taiji Nishi ,  Motohiro Fukuda ,  Yasuzo Kirita

发明人： Go Tazaki ,  Taiji Nishi ,  Motohiro Fukuda ,  Yasuzo Kirita

IPC分类号： C12M3/00

CPC分类号： C12M25/06 ,  C12M23/10 ,  C12M23/22 ,  C12M23/38 ,  C12M25/14

摘要： It is intended to provide a cell culture chamber in a shape suitable for cell culture. It is also intended to provide a cell culture chamber which is inexpensive and facilitates observation. A cell culture chamber (100) includes a cover (10), a chamber body (20) and a plate-shaped base (30). The cover (10) covers an opening (21) of the chamber body (20). The chamber body (20) contains a culture such as cells. The plate base (30) covers a through hole (24) formed at the bottom (23) of the chamber body (20). Moreover, the plate base (30) has fine pits and projections formed thereon for culturing the culture.

摘要翻译： 旨在提供一种适于细胞培养的形状的细胞培养室。 也是为了提供廉价且便于观察的细胞培养室。 细胞培养室（100）包括盖（10），室主体（20）和板状基部（30）。 盖（10）覆盖室主体（20）的开口（21）。 腔体（20）包含诸如细胞的培养物。 板基座（30）覆盖形成在室主体（20）的底部（23）处的通孔（24）。 此外，板基（30）在其上形成有细微的凹坑和突起，用于培养培养物。

公开(公告)号：US08435782B2

公开(公告)日：2013-05-07

申请号：US12280607

申请日：2007-02-19

申请人： Taiji Nishi ,  Go Tazaki ,  Motohiro Fukuda ,  Michio Yazawa ,  Masayuki Takahashi

发明人： Taiji Nishi ,  Go Tazaki ,  Motohiro Fukuda ,  Michio Yazawa ,  Masayuki Takahashi

IPC分类号： C12M1/14

CPC分类号： C12M23/12 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2300/0893 ,  C12M25/00

摘要： An object of the present invention is to provide a cell culture chamber with which the survival rate of cells to be cultured can be increased, and a method of producing the same. A cell culture chamber according to the present invention includes a mass of projections formed of a plurality of microprojections 1 formed on a surface on which cells are cultured. The width or diameter of each of the microprojections 1 is in a range of 20 nm to 3 μm, and the aspect ratio of each of the microprojections is in a range of 0.2 to 3.0. Thus, there can be provided a cell culture chamber most suitable for the adhesion and differentiation/proliferation of cells to be cultured.

摘要翻译： 本发明的目的是提供一种能够提高培养细胞存活率的细胞培养室及其制造方法。 根据本发明的细胞培养室包括由在其上培养细胞的表面上形成的多个微突出物1形成的突起体。 每个微喷射体1的宽度或直径在20nm至3μm的范围内，并且每个微喷射体的纵横比在0.2至3.0的范围内。 因此，可以提供最适合于待培养细胞的粘附和分化/增殖的细胞培养室。

公开(公告)号：US20120199816A1

公开(公告)日：2012-08-09

申请号：US13390271

申请日：2010-08-02

申请人： Go Tazaki ,  Motohiro Fukuda ,  Hiroyuki Ohgi ,  Akio Fujita

发明人： Go Tazaki ,  Motohiro Fukuda ,  Hiroyuki Ohgi ,  Akio Fujita

IPC分类号： H01L51/54 ,  H01L51/48 ,  H01L51/46 ,  H01L51/56

CPC分类号： H01L51/4253 ,  H01L51/0026 ,  H01L51/0036 ,  H01L51/0037 ,  H01L51/424 ,  H01L51/447 ,  H01L2251/105 ,  H01L2251/308 ,  Y02E10/549 ,  Y02P70/521

摘要： A photoelectric conversion device according to the present invention includes, between a pair of electrodes, an electron donor layer having an interdigitated shape in cross section comprising a stripe-like part in cross section and a base, a plurality of strip-like parts in cross section extending in a direction intersecting electrode main surfaces being formed at intervals in the stripe-like part in cross section; and an electron acceptor layer having an interdigitated shape in cross section comprising a stripe-like part in cross section and a base, a plurality of strip-like parts in cross section extending in a direction intersecting the electrode main surfaces being formed at intervals in the stripe-like part in cross section, the photoelectric conversion device further including an active layer in which the plurality of strip-like parts in cross section of the electron donor layer and the plurality of strip-like parts in cross section of the electron acceptor layer are alternately joined. A stripe width a of the stripe-like part in cross section of the electron donor layer and a stripe width b of the stripe-like part in cross section of the electron acceptor layer are both 5 to 100 nm. When a=b, a thickness c of the active layer is twice to 40 times as large as a (=b). When a≠b, the thickness c of the active layer is twice or more of one of a and b which is smaller and 40 times or less of one of a and b which is larger.

摘要翻译： 根据本发明的光电转换装置包括在一对电极之间，具有横截面的交错形状的电子供体层，包括横截面的条状部分和基部，多个条状部分交叉 横截面中在与条状部分间隔地形成与电极主表面交叉的方向延伸的部分; 以及具有交叉形状的电子受体层，其截面包括条状部分和基部，沿着与电极主表面相交的方向延伸的多个条状部分形成在 光电转换装置还包括有源层，其中电子供体层的横截面中的多个条状部分和电子受体层的横截面中的多个条状部分 交替连接。 电子供体层的横截面中的条状部分的条纹宽度a和电子受体层横截面中的条状部分的条带宽度b均为5〜100nm。 当a = b时，有源层的厚度c为（= b）的两倍至40倍。 当a≠b时，有效层的厚度c是a和b中的一个的两倍或更多，其中a和b之一更小，并且a和b之一更大的是40倍或更少。

公开(公告)号：US20110217725A1

公开(公告)日：2011-09-08

申请号：US13125896

申请日：2009-10-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

发明人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

IPC分类号： C12N5/07 ,  C12Q1/02

CPC分类号： C12N5/0062 ,  C12M23/12 ,  C12N2502/00 ,  C12N2503/02 ,  C12N2513/00

摘要： To provide a cell culture kit including cultured living cells of various donors, and a manufacturing method thereof. The cell culture kit includes a culture plate and living cells cultured thereon. The culture plate includes a plurality of microchambers (33) and living cells derived from various donors are adhered to surfaces of the plurality of microchambers (33). Specifically, living cells D1, D2, and D3 derived from various donors are adhered to the plurality of microchambers (33). In each microchamber (33), living cells derived from one donor or living cells derived from various donors may be cultured. The living cells derived from various donors are adhered and cultured in the cell culture kit as a whole, which makes it possible to provide a cell culture kit to conduct a test using cells derived from various donors.

摘要翻译： 提供包括各种供体的培养的活细胞的细胞培养试剂盒及其制造方法。 细胞培养试剂盒包括培养板和在其上培养的活细胞。 培养板包括多个微室（33），并且来自各种供体的活细胞粘附到多个微室（33）的表面。 具体而言，将来自各种供体的活细胞D1，D2和D3粘附到多个微室（33）。 在每个微室（33）中，可以培养衍生自一个供体的活细胞或来自各种供体的活细胞。 来自各种供体的活细胞作为整体在细胞培养试剂盒中附着和培养，这使得可以提供使用来自各种供体的细胞进行测试的细胞培养试剂盒。

公开(公告)号：US20110207215A1

公开(公告)日：2011-08-25

申请号：US13125871

申请日：2009-10-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

发明人： Yoko Itchoda ,  Go Tazaki ,  Motohiro Fukuda ,  Hitoshi Tsuruta ,  Hideki Taniguchi

IPC分类号： C12N5/071 ,  C12N5/0735 ,  C12N5/0793 ,  C12N5/077

CPC分类号： A01N1/0263 ,  C12M23/12 ,  C12M23/24 ,  C12M23/38 ,  C12M23/54 ,  C12M45/22

摘要： To provide a cell storage method to store living cells while maintaining the functions of the living cells. An aspect of the cell storage method is a method to store living cells (40) by using a cell culture chamber (20) including a plurality of the microchambers (11), the method including: culturing the living cells by being adhered to the surfaces of a plurality of micro spaces; and after the culturing, pouring the culture medium (50) into the cell culture chamber (20) so as to cover the plurality of microchambers (11), and storing the living cells. The living cells are adhered to a cell culture chamber of an appropriate size and cultured to form a three-dimensional structure. This enables maintaining the three-dimensional structure of the living cells and storage of the living cells while maintaining the functions thereof.

摘要翻译： 提供细胞储存方法来储存活细胞同时保持活细胞的功能。 细胞储存方法的一个方面是通过使用包括多个微室（11）的细胞培养室（20）来储存活细胞（40）的方法，所述方法包括：通过粘附到所述表面上培养活细胞 的多个微空间; 培养后，将培养基（50）注入到细胞培养室（20）中以覆盖多个微室（11），并储存活细胞。 将活细胞粘附到适当尺寸的细胞培养室并进行培养以形成三维结构。 这能够维持活细胞的三维结构并维持活体的功能。

公开(公告)号：US10513685B2

公开(公告)日：2019-12-24

申请号：US13636855

申请日：2011-03-23

申请人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

发明人： Yoko Itchoda ,  Go Tazaki ,  Masaya Hosoda ,  Motohiro Fukuda ,  Hideki Taniguchi ,  Yun-Wen Zheng ,  Keisuke Sekine

IPC分类号： C12N5/071 ,  C12N5/0735 ,  C12M1/32

摘要： Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surface formed of spaces in which the microchambers (11) have a space structure with a height of 10 μm to 500 μm and a bottom area of 100 μm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers (10).