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    System and method for detection of HLA Variants
    1.
    发明申请
    System and method for detection of HLA Variants 审中-公开
    HLA变异体检测系统及方法

    公开(公告)号：US20100261189A1

    公开(公告)日：2010-10-14

    申请号：US12798877

    申请日：2010-04-12

    申请人： Gordon Bentley ,  Henry A. Erlich ,  Russell Gene Higuchi ,  Cherie Holcomb

    发明人： Gordon Bentley ,  Henry A. Erlich ,  Russell Gene Higuchi ,  Cherie Holcomb

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6881 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2535/122

    摘要： A method for detecting one or more HLA sequence types is described that comprises the steps of: amplifying a plurality of first amplicons from a double stranded nucleic acid sample, wherein the first amplicons are amplified with a plurality of pairs of nucleic acid primers that define exons 2 and 3 of both strands of HLA loci from the group consisting of HLA-A, HLA-B, and HLA-C; amplifying the first amplicons to produce a plurality of populations of second amplicons, wherein each population of second amplicons is clonally amplified from one of the first amplicons; sequencing the plurality of populations of second amplicons to generate a nucleic acid sequence composition for each of the plurality of second amplicons; and detecting variation in the sequence composition from one or more of the second amplicons for one or more of the HLA loci.

    摘要翻译： 描述了检测一种或多种HLA序列类型的方法，其包括以下步骤：从双链核酸样品扩增多个第一扩增子，其中第一扩增子用定义外显子的多对核酸引物扩增 来自HLA-A，HLA-B和HLA-C组的HLA基因座的两条链中的2和3个; 扩增第一扩增子以产生多个第二扩增子群体，其中第二扩增子的每个群体从第一扩增子之一克隆扩增; 对多个第二扩增子群进行测序以产生用于多个第二扩增子中的每一个的核酸序列组合物; 以及从一个或多个HLA基因座的一个或多个第二扩增子检测序列组成的变化。

    Method for HLA DP typing
    7.
    发明授权
    Method for HLA DP typing 失效
    HLA DP分型方法

    公开(公告)号：US5541065A

    公开(公告)日：1996-07-30

    申请号：US195615

    申请日：1994-02-14

    申请人： Henry A. Erlich ,  Glenn T. Horn ,  Teodorica Bugawan ,  Ann B. Begovich

    发明人： Henry A. Erlich ,  Glenn T. Horn ,  Teodorica Bugawan ,  Ann B. Begovich

    IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K49/00 ,  C07K14/74 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566 ,  C07H21/04

    CPC分类号： C12Q1/6881 ,  C07K14/70539 ,  C12Q1/6811 ,  C12Q2600/156

    摘要： A process for determining the genotype of an individual with respect to the alleles at the HLA DP locus involves obtaining a sample of nucleic acid from the individual, and hybridizing the nucleic acids with a panel of probes specific for variant segments of DPalpha and DPbeta genes. Because the variation between DPbeta alleles is highly dispersed throughout the second exon of the DPbeta gene, the discovery of many different DPbeta alleles makes the process far more discriminating and informative than cellular, RFLP, or serological methods. The process can also be carried out on amplified nucleic acid produced by the polymerase chain reaction using primers specific for the second exon of the DPalpha and DPbeta genes. HLA DP DNA typing methods are useful in the prevention of graft rejection and host versus graft disease, in determining susceptibility to autoimmune diseases, in providing evidence concerning the derivation from an individual of forensic samples, and in paternity testing.

    摘要翻译： 用于确定个体相对于HLA DP位点处的等位基因的基因型的方法包括从个体获得核酸样品，并将核酸与DPalpha和DPbeta基因的变体片段特异性的一组探针杂交。 因为DPbeta等位基因之间的差异在DPbeta基因的第二个外显子中高度分散，许多不同的DPbeta等位基因的发现使得该过程比细胞，RFLP或血清学方法更具歧视性和信息性。 该方法还可以通过聚合酶链式反应产生的扩增核酸进行，其使用对DPalpha和DPbeta基因的第二外显子特异的引物。 HLA DP DNA分型方法可用于预防移植排斥反应和宿主与移植物疾病，确定对自身免疫性疾病的易感性，提供有关法医样本个体的推导和亲子鉴定的证据。

    Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
    9.
    发明授权
    Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme 失效
    使用热稳定酶扩增，检测和/或克隆核酸序列的方法

    公开(公告)号：US4965188A

    公开(公告)日：1990-10-23

    申请号：US63647

    申请日：1987-06-17

    申请人： Kary B. Mullis ,  Henry A. Erlich ,  David H. Gelfand ,  Glenn Horn ,  Randall K. Saiki

    发明人： Kary B. Mullis ,  Henry A. Erlich ,  David H. Gelfand ,  Glenn Horn ,  Randall K. Saiki

    IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/70

    CPC分类号： C12P19/34 ,  C12N9/1252 ,  C12Q1/6844

    摘要： A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The amplified sequence can be readily detected. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.

    摘要翻译： 用于扩增核酸或其混合物中包含的任何靶核酸序列的方法包括用摩尔过量的两个寡核苷酸引物处理所述核酸的分开的互补链，并用热稳定酶扩增引物以形成互补引物延伸产物，其起作用 作为合成所需核酸序列的模板。 可以容易地检测扩增的序列。 反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。

    Characterization and detection of sequences associated with autoimmune diseases
    10.
    发明授权
    Characterization and detection of sequences associated with autoimmune diseases 失效
    表征和检测与自身免疫疾病相关的序列

    公开(公告)号：US5665548A

    公开(公告)日：1997-09-09

    申请号：US448021

    申请日：1995-05-23

    申请人： Henry A. Erlich ,  Glenn T. Horn

    发明人： Henry A. Erlich ,  Glenn T. Horn

    IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/395 ,  A61P37/00 ,  C07K14/74 ,  C07K16/28 ,  C12N15/02 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/564 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6883 ,  C07K14/70539 ,  C07K16/2833 ,  C12Q1/686 ,  C12Q1/6881 ,  A61K38/00 ,  C12Q2600/156 ,  C12Q2600/172

    摘要： DNA sequences and corresponding amino acid sequences from the HLA class II beta region of the human genome that are associated with insulin-dependent diabetes mellitus (IDDM) and Pemphigus vulgaris (PV) have been identified. Specifically, marker DNA sequences which detect either directly or indirectly the identity of the codon encoding for the amino acid at position 57 of the DQ.beta. protein sequence are disclosed as well as sequences from the DR.beta. region. These sequences may be used to generate DNA hybridization probes and antibodies for assays to detect a person's susceptibility to autoimmune diseases, such as IDDM and PV. Such antibodies and peptides encoded by said DNA sequences can be used therapeutically or prophylactically.

    摘要翻译： 已经鉴定了与胰岛素依赖性糖尿病（IDDM）和寻常型天疱疮（PV）相关的人类基因组的HLA II类β区的DNA序列和相应的氨基酸序列。 具体地，公开了直接或间接检测编码DQβ蛋白序列的位置57的氨基酸的密码子的身份的标记DNA序列，以及来自DRβ区的序列。 这些序列可用于产生DNA杂交探针和用于检测个体对自身免疫性疾病如IDDM和PV的易感性的测定的抗体。 由所述DNA序列编码的这样的抗体和肽可以用于治疗或预防。