Preparation of romidepsin
    3.
    发明授权
    Preparation of romidepsin 有权
    罗非曲汀的制备

    公开(公告)号:US08691534B2

    公开(公告)日:2014-04-08

    申请号:US11966258

    申请日:2007-12-28

    IPC分类号: C12P21/04

    CPC分类号: C07K5/101

    摘要: The invention provides an improved process for preparing romidepsin. The process involves producing, purifying, or storing romidepsin under conditions that prevent the formation of undesired adducts. Purifying romidepsin at an apparent pH lower than approximately 6.0 (e.g., between an apparent pH of 4.0 and 6.0) has been discovered to prevent the reduction of the disulfide bond of romidepsin and the subsequent formation of dimerized, oligomerized, or polymerized adducts. The invention also provides compositions of monomeric romidepsin free of dimerized, oligomerized, or polymerized adducts.

    摘要翻译: 本发明提供了一种制备罗美甜素的改进方法。 该方法包括在防止形成不需要的加合物的条件下生产,纯化或存储罗非曲松。 已经发现在表观pH低于约6.0(例如,表观pH为4.0和6.0之间)的情况下纯化罗非曲松,以防止罗非替辛的二硫键的还原和随后形成二聚,低聚或聚合的加合物。 本发明还提供了不含二聚,低聚或聚合的加合物的单体罗非替克组合物。

    PREPARATION OF ROMIDEPSIN
    5.
    发明申请
    PREPARATION OF ROMIDEPSIN 审中-公开
    ROMIDEPSIN的制备

    公开(公告)号:US20090209616A1

    公开(公告)日:2009-08-20

    申请号:US12420451

    申请日:2009-04-08

    IPC分类号: A61K31/39 A61P43/00

    CPC分类号: C07K5/101

    摘要: The invention provides an improved process for preparing romidepsin. The process involves producing, purifying, or storing romidepsin under conditions that prevent the formation of undesired adducts. Purifying romidepsin at an apparent pH lower than approximately 6.0 (e.g., between an apparent pH of 4.0 and 6.0) has been discovered to prevent the reduction of the disulfide bond of romidepsin and the subsequent formation of dimerized, oligomerized, or polymerized adducts. The invention also provides compositions of monomeric romidepsin free of dimerized, oligomerized, or polymerized adducts.

    摘要翻译: 本发明提供了一种制备罗美甜素的改进方法。 该方法包括在防止形成不需要的加合物的条件下生产,纯化或存储罗非曲松。 已经发现在表观pH低于约6.0(例如,表观pH为4.0和6.0之间)的情况下净化罗非曲松,以防止罗非替辛的二硫键的还原和随后形成二聚,低聚或聚合的加合物。 本发明还提供了不含二聚,低聚或聚合的加合物的单体罗非替克组合物。

    Uridine analogs and techniques for making and using

    公开(公告)号:US06987180B2

    公开(公告)日:2006-01-17

    申请号:US10072641

    申请日:2002-02-07

    摘要: Uridine analogs and techniques for making and using uridine analogs are disclosed in this invention. These uridine analogs include nucleoside phosphates having a 5-aminouracil group. These nucleotides can be incorporated into a nucleic acid as an unnatural base, as a substitute for uridine or thymine. The nucleic acid can then be treated with an oxidizing agent and an alkaline solution, which causes cleavage of the nucleic acid at the position of the unnatural base. The nucleoside phosphate analogs can be used in many ways, including measuring chemical interactions between nucleic acids and other compounds, or sequencing nucleic acids. Additional compounds can also be derivitized onto the amino group, allowing other functionalities to be added to the nucleoside phosphate, or to the nucleic acid incorporating the nucleoside phosphate.

    Template-directed interference footprinting of protein-adenine contacts
    7.
    发明授权
    Template-directed interference footprinting of protein-adenine contacts 有权
    模板导向的蛋白质 - 腺嘌呤接触的干扰足迹

    公开(公告)号:US06312897B1

    公开(公告)日:2001-11-06

    申请号:US09214207

    申请日:1999-11-04

    IPC分类号: C12Q168

    摘要: A chemical analog of adenine is provided which can be incorporated into DNA but base pairs as normal, and does not disrupt DNA secondary structure. The analog is cleavable and disrupts functionality with DNA-binding protein. The analog finds use in a technique provided by the invention involving TDI footprinting or adenine-DNA contacts.

    摘要翻译: 提供腺嘌呤的化学类似物,其可以正常并入DNA但碱基对,并且不破坏DNA二级结构。 该类似物可裂解并破坏DNA结合蛋白的功能。 该类似物用于本发明提供的涉及TDI足迹或腺嘌呤-DNA接触的技术中。

    Selection of binding-molecules
    8.
    发明授权
    Selection of binding-molecules 失效
    选择结合分子

    公开(公告)号:US5783384A

    公开(公告)日:1998-07-21

    申请号:US220272

    申请日:1994-03-30

    IPC分类号: C07K1/22 C12Q1/68

    CPC分类号: C07K1/22 C12Q1/6811

    摘要: Methods of designing and producing sequence-specific DNA binding proteins, methods of determining the affinity of a specific binding molecule for a target and products produced by these methods are disclosed. The methods include: forming a reversible bond between a specific binding molecule and the target and determining the susceptibility of the reversible bond to reversal as a measure of the affinity of the binding molecule for the target.

    摘要翻译: 公开了设计和产生序列特异性DNA结合蛋白的方法,确定特异性结合分子对靶标的亲和力和通过这些方法产生的产物的方法。 所述方法包括:在特异性结合分子与靶标之间形成可逆键,并确定可逆键转化的易感性,作为结合分子对靶标的亲和力的量度。

    Stabilized MAML peptides and uses thereof
    9.
    发明授权
    Stabilized MAML peptides and uses thereof 有权
    稳定的MAML肽及其用途

    公开(公告)号:US09074009B2

    公开(公告)日:2015-07-07

    申请号:US12478504

    申请日:2009-06-04

    IPC分类号: C07K7/00 A61K38/00 C07K14/47

    CPC分类号: C07K14/47 A61K38/00

    摘要: Stably cross-linked a polypeptides related to human MAML are described. These cross-linked polypeptides contain at least two modified amino acids that together form an internal cross-link or tether that can help to stabilize the alpha-helical secondary structure that is thought to be important for binding of MAML peptides to the Notch transcription complex, a complex that includes ICN and CSL.

    摘要翻译: 描述了稳定交联的与人MAML相关的多肽。 这些交联的多肽含有至少两个修饰的氨基酸,它们一起形成可以有助于稳定被认为对MAML肽与Notch转录复合物的结合重要的α-螺旋二级结构的内部交联或系链, 一个包含ICN和CSL的复合体。