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    Aldolases, nucleic acids encoding them and methods for making and using them
    5.
    发明申请
    Aldolases, nucleic acids encoding them and methods for making and using them 有权
    醛缩酶，编码它们的核酸和制备和使用它们的方法

    公开(公告)号：US20080020435A1

    公开(公告)日：2008-01-24

    申请号：US11714909

    申请日：2007-03-07

    申请人： Ellen Burke ,  Steven Gort ,  Paula Hicks ,  Peter Luginbuhl ,  Sara McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

    发明人： Ellen Burke ,  Steven Gort ,  Paula Hicks ,  Peter Luginbuhl ,  Sara McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

    IPC分类号： C12P17/10

    CPC分类号： C12P17/10 ,  C12N9/88 ,  C12P13/001 ,  Y02P20/52

    摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

    摘要翻译： 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可以用于各种药物，农业和工业环境。 在一些实施方案中，本发明提供了可用于制备R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体的多肽和生物合成途径，例如R ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的某些立体异构体，例如R，R和S，R构型及其盐。