公开(公告)号：US6021344A

公开(公告)日：2000-02-01

申请号：US984391

申请日：1997-12-03

申请人： Harvey Lui ,  Haishan Zeng ,  Calum E. MacAulay ,  Branko Palcic ,  David I. McLean

发明人： Harvey Lui ,  Haishan Zeng ,  Calum E. MacAulay ,  Branko Palcic ,  David I. McLean

IPC分类号： A61B5/00 ,  A61B5/103 ,  A61B10/00

CPC分类号： A61B5/444 ,  A61B5/0059 ,  A61B5/0071 ,  A61B2090/502

摘要： Apparatus for the diagnosis of a skin disease site by visual fluorescence inspection comprising an excitation light source for illuminating the disease site, a light guide for transmitting the excitation light directly to the disease site to generate fluorescence light and viewing goggles for processing the excitation light reflected and the fluorescence light emitted from the disease site to provide a fluorescence image of the disease site to a user. The fluorescence image is used to aid the medical assessment of skin conditions and the diagnosis of cutaneous diseases by supplementing the visual assessment of skin lesions made by the naked eye. The apparatus can be used in several modes of operation that permit the viewing of full color fluorescence images and enhanced two color images. The apparatus can also use image intensifying equipment to amplify fluorescence light so that even very weak fluorescing objects can be seen. A method for acquiring and viewing the fluorescence images is also disclosed.

摘要翻译： 用于通过视觉荧光检查诊断皮肤疾病部位的装置，包括用于照射疾病部位的激发光源，用于将激发光直接传播到疾病部位以产生荧光的光导和观察用于处理反射的激发光的护目镜 以及从疾病部位发出的荧光，向用户提供疾病部位的荧光图像。 荧光图像用于通过补充肉眼皮肤损伤的视觉评估来帮助对皮肤状况的医学评估和皮肤疾病的诊断。 该装置可以用于允许观看全色荧光图像和增强的双色图像的几种操作模式。 该装置还可以使用图像增强设备来放大荧光，使得甚至可以看到非常弱的荧光物体。 还公开了一种用于获取和观察荧光图像的方法。

公开(公告)号：US6069689A

公开(公告)日：2000-05-30

申请号：US59885

申请日：1998-04-14

申请人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

发明人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

IPC分类号： A61B5/00 ,  A61B5/103 ,  G01N21/64 ,  G01N21/65 ,  G01B9/02 ,  G01N11/00

CPC分类号： G01N21/474 ,  A61B5/0059 ,  A61B5/0071 ,  A61B5/444 ,  G01N21/645 ,  G01N21/6486 ,  G01N21/65 ,  G01N2021/475 ,  G01N2021/6417 ,  G01N2021/656 ,  G01N2201/0627

摘要： Apparatus for diagnosis of a skin disease site using spectral analysis includes a light source for generating light to illuminate the disease site and a probe unit optically connected to the light source for exposing the disease site to light to generate fluorescence and reflectance light. The probe unit also collects the generated fluorescence and reflectance light and transmits this light to a spectrometer to be analyzed. The spectrometer generates and displays spectral measurements of the fluorescence light and the reflectance light which in together assist the user in diagnosing the disease site. The apparatus makes use of a conventional personal computer using a plug-in spectrometer card to provide a compact and low costs system. The system performs combined fluorescence and reflectance spectral analysis in a quick and efficient manner to provide a powerful tool for dermatologic diagnosis.

摘要翻译： 用于使用光谱分析诊断皮肤疾病部位的装置包括用于产生照亮疾病部位的光的光源和与光源光学连接的探针单元，用于将疾病部位暴露于光以产生荧光和反射光。 探针单元还收集产生的荧光和反射光，并将该光传输到要分析的光谱仪。 光谱仪产生和显示荧光和反射光的光谱测量，这些在一起协助用户诊断疾病部位。 该装置利用使用插入式光谱仪卡的常规个人计算机来提供紧凑且低成本的系统。 该系统以快速有效的方式进行组合荧光和反射光谱分析，为皮肤病诊断提供强大的工具。

公开(公告)号：US6128525A

公开(公告)日：2000-10-03

申请号：US901999

申请日：1997-07-29

申请人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

发明人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

IPC分类号： A61B18/20 ,  A61N5/06 ,  A61N5/067 ,  A61B6/00

CPC分类号： A61N5/062 ,  A61B1/043 ,  A61B18/20 ,  A61B2018/00904 ,  A61N2005/0644 ,  A61N2005/067

摘要： A method and apparatus for controlling the dosimetry of a photodynamic therapy that involves exposing a site to be treated to treatment light in order to generate toxic products at the site and other photoproducts. Often a photosensitizer drug is administered to the patient prior to treatment or the therapy relies on the presence of endogenous photosensitizers. The method comprises the steps of selecting a photoproduct having an identifying characteristic, which can be a fluorescence peak, and monitoring the photoproduct using the identifying characteristic (e.g. fluorescence) to determine the level of the photoproduct being generated. The photodynamic therapy is then terminated when the photoproduct being monitored reaches a predetermined level. The method allows for safe treatment of a site using photodynamic therapy and ensures that overexposure to treatment light leading to damage of normal tissue or underexposure leading to ineffective treatment of the lesion does not occur. Apparatus for automatically carrying out the method is also provided.

摘要翻译： 一种用于控制光动力疗法的剂量测定的方法和装置，其涉及将待治疗的部位暴露于治疗光，以便在该部位产生有毒产物和其它光产物。 在治疗之前通常将光敏剂药物施用于患者，或者治疗依赖于内源性光敏剂的存在。 该方法包括以下步骤：选择具有识别特征的光产物，其可以是荧光峰，并使用识别特征（例如荧光）监测光产物，以确定所产生的光产物的水平。 当被监测的光产物达到预定水平时，光动力疗法终止。 该方法允许使用光动力疗法安全治疗部位，并且确保不会发生过度暴露于治疗光，导致正常组织损伤或曝光不足导致病变无效治疗。 还提供了用于自动执行该方法的装置。

公开(公告)号：US6008889A

公开(公告)日：1999-12-28

申请号：US843605

申请日：1997-04-16

申请人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

发明人： Haishan Zeng ,  Harvey Lui ,  Calum MacAulay ,  Branko Palcic ,  David I. McLean

IPC分类号： A61B5/00 ,  A61B5/103 ,  G01N21/64 ,  G01N21/65 ,  G01B9/02 ,  G01N11/00

CPC分类号： G01N21/474 ,  A61B5/0059 ,  A61B5/0071 ,  A61B5/444 ,  G01N21/645 ,  G01N21/6486 ,  G01N21/65 ,  G01N2021/475 ,  G01N2021/6417 ,  G01N2021/656 ,  G01N2201/0627

摘要： Apparatus for diagnosis of a skin disease site using spectral analysis includes a light source for generating light to illuminate the disease site and a probe unit optically connected to the light source for exposing the disease site to light to generate fluorescence and reflectance light. The probe unit also collects the generated fluorescence and reflectance light and transmits this light to a spectrometer to be analysed. The spectrometer generates and displays spectral measurements of the fluorescence light and the reflectance light which in together assist the user in diagnosing the disease site. The apparatus makes use of a conventional personal computer using a plug-in spectrometer card to provide a compact and low cost system. The system performs combined fluorescence and reflectance spectral analysis in a quick and efficient manner to provide a powerful tool for dermatologic diagnosis.

摘要翻译： 用于使用光谱分析诊断皮肤疾病部位的装置包括用于产生照亮疾病部位的光的光源和与光源光学连接的探针单元，用于将疾病部位暴露于光以产生荧光和反射光。 探针单元还收集产生的荧光和反射光，并将该光传输到要分析的光谱仪。 光谱仪产生和显示荧光和反射光的光谱测量，这些在一起协助用户诊断疾病部位。 该装置利用使用插入式光谱仪卡的常规个人计算机来提供紧凑且低成本的系统。 该系统以快速有效的方式进行组合荧光和反射光谱分析，为皮肤病诊断提供强大的工具。

公开(公告)号：US20080221457A1

公开(公告)日：2008-09-11

申请号：US10596072

申请日：2004-11-26

申请人： Haishan Zeng ,  Harvey Lui ,  Zhiwei Huang ,  David I. McLean

发明人： Haishan Zeng ,  Harvey Lui ,  Zhiwei Huang ,  David I. McLean

IPC分类号： A61B6/00

CPC分类号： A61B5/0071 ,  A61B5/0035 ,  A61B5/0075 ,  A61B5/0086 ,  A61B5/444 ,  A61B5/7264 ,  G01N21/6486 ,  G01N21/65

摘要： Methods and apparatus for classifying tissue use features of Raman spectra and background fluorescent spectra. The spectra may be acquired in the near-infrared wavelengths. Principal component analysis and linear discriminant analysis of reference spectra may be used to obtain a classification function that accepts features of the Raman and background fluorescence spectra for test tissue and yields an indication as to the likelihood that the test tissue is abnormal. The methods and apparatus may be applied to screening for skin cancers or other diseases.

摘要翻译： 用于分类拉曼光谱和背景荧光光谱的组织使用特征的方法和装置。 可以在近红外波长中获取光谱。 可以使用参考光谱的主成分分析和线性判别分析来获得接受测试组织的拉曼特征和背景荧光光谱的分类函数，并产生关于测试组织异常的可能性的指示。 所述方法和装置可用于筛选皮肤癌或其它疾病。

公开(公告)号：US08326404B2

公开(公告)日：2012-12-04

申请号：US10596072

申请日：2004-11-26

申请人： Haishan Zeng ,  Harvey Lui ,  Zhiwei Huang ,  David I. McLean

发明人： Haishan Zeng ,  Harvey Lui ,  Zhiwei Huang ,  David I. McLean

IPC分类号： A61B6/00

CPC分类号： A61B5/0071 ,  A61B5/0035 ,  A61B5/0075 ,  A61B5/0086 ,  A61B5/444 ,  A61B5/7264 ,  G01N21/6486 ,  G01N21/65

摘要： Methods and apparatus for classifying tissue use features of Raman spectra and background fluorescent spectra. The spectra may be acquired in the near-infrared wavelengths. Principal component analysis and linear discriminant analysis of reference spectra may be used to obtain a classification function that accepts features of the Raman and background fluorescence spectra for test tissue and yields an indication as to the likelihood that the test tissue is abnormal. The methods and apparatus may be applied to screening for skin cancers or other diseases.

摘要翻译： 用于分类拉曼光谱和背景荧光光谱的组织使用特征的方法和装置。 可以在近红外波长中获取光谱。 可以使用参考光谱的主成分分析和线性判别分析来获得接受测试组织的拉曼特征和背景荧光光谱的分类函数，并产生关于测试组织异常的可能性的指示。 所述方法和装置可用于筛选皮肤癌或其它疾病。

公开(公告)号：US06269818B1

公开(公告)日：2001-08-07

申请号：US09084865

申请日：1998-05-26

申请人： Harvey Lui ,  Calum Macaulay ,  Haishan Zeng ,  David I. McLean ,  Robert Bissonnette

发明人： Harvey Lui ,  Calum Macaulay ,  Haishan Zeng ,  David I. McLean ,  Robert Bissonnette

IPC分类号： A61B1900

CPC分类号： A61K49/0036 ,  A61K41/0071 ,  A61K49/0017 ,  A61N5/062

摘要： In one aspect, the invention provides a diagnostic method for identifying psoriatic plaques in which Porphyrins, particularly protoporphyrin IX, are elevated as compared to normal skin and skin of patients with other dermatological diseases, including other forms of psoriatic plaque. Psoriatic plaques with elevated porphyrin levels may be detected by fluorescence and spectral analysis. Endogenous porphyrins in psoriatic plaques may be activated with visible light to treat psoriatic plaques having elevated porphyrin concentrations. Skin conditions may be optimized to increase the endogenous concentration of porphyrins in psoriatic plaques. A topical formulation may be applied to psoriatic plaques to optimize skin conditions such as pH, iron concentration, temperature, hydration, calcium concentration, oxygenation, electrical conductivity and estrogen concentration to increase the concentration of endogenous porphyrins.

摘要翻译： 一方面，本发明提供了一种鉴别银屑病斑块的诊断方法，其中卟啉，特别是原卟啉IX，与具有其他皮肤病学疾病，包括其他形式的银屑病斑块的患者的正常皮肤和皮肤相比升高。 通过荧光和光谱分析可以检测到卟啉水平升高的银屑病斑块。 银屑病斑块中的内源性卟啉可以用可见光激活以治疗具有升高的卟啉浓度的银屑病斑块。 可以优化皮肤状况以增加银屑病斑块中卟啉的内源性浓度。 局部制剂可以应用于银屑病斑块以优化皮肤状况，例如pH，铁浓度，温度，水合，钙浓度，氧合，电导率和雌激素浓度以增加内源卟啉的浓度。

公开(公告)号：US20080123106A1

公开(公告)日：2008-05-29

申请号：US11722878

申请日：2005-12-23

申请人： Haishan Zeng ,  Lioudmila Tchvialeva ,  Tim K. Lee ,  David I. McLean ,  Harvey Lui

发明人： Haishan Zeng ,  Lioudmila Tchvialeva ,  Tim K. Lee ,  David I. McLean ,  Harvey Lui

IPC分类号： G01B11/30 ,  G01B11/02 ,  G06F19/00 ,  A61B5/00

CPC分类号： A61B5/0066 ,  A61B5/444 ,  A61B5/445 ,  G01B11/303 ,  G01J9/02 ,  G01N21/47 ,  G01N2021/479

摘要： Surface roughness measurements are made by illuminating a surface with coherent light to generate a speckle pattern and studying characteristics of the speckle pattern. The disclosed techniques may be applied to measuring the surface roughness of skin or other biological surfaces. Skin roughness information may be used in the diagnosis of conditions such as malignant melanoma. Methods and apparatus for measuring the coherence length of optical sources involve extracting information about speckle patterns resulting when light from the optical sources interacts with a surface having a known roughness.

摘要翻译： 通过用相干光照射表面以产生斑点图案并研究斑点图案的特征来进行表面粗糙度测量。 所公开的技术可以用于测量皮肤或其他生物表面的表面粗糙度。 皮肤粗糙度信息可用于诊断恶性黑色素瘤等病症。 用于测量光源的相干长度的方法和装置包括提取关于来自光源的光与具有已知粗糙度的表面相互作用的斑点图案的信息。

公开(公告)号：US09687152B2

公开(公告)日：2017-06-27

申请号：US14009473

申请日：2012-04-05

申请人： Haishan Zeng ,  Harvey Lui ,  David McLean ,  Anthony Lee ,  Hequn Wang ,  Shuo Tang

发明人： Haishan Zeng ,  Harvey Lui ,  David McLean ,  Anthony Lee ,  Hequn Wang ,  Shuo Tang

IPC分类号： A61B5/00 ,  A61B5/06 ,  G02B21/00 ,  G02B21/06 ,  G01J3/44 ,  G01J3/02 ,  A61B18/18 ,  A61N5/06

CPC分类号： A61B5/0064 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/4836 ,  A61B5/6831 ,  A61B5/6832 ,  A61B18/18 ,  A61N5/06 ,  A61N5/0616 ,  G01J3/0218 ,  G01J3/027 ,  G01J3/44 ,  G02B21/0052 ,  G02B21/0076 ,  G02B21/06

摘要： A multiphoton microscope is provided. The microscope includes: an excitation source for providing an optical excitation beam at an excitation wavelength λ; a scanner for scanning the excitation beam on a sample; an objective for irradiating the sample with the excitation beam scanned by the scanner and for collecting an emission beam from the sample; a first detector for detecting a plurality of multiphoton signals; and an emission light path allowing transmission from the objective to the first detector a wavelength band limited to greater than or equal to λ/2 and less than λ, wherein the plurality of multiphoton signals have wavelengths within the wavelength band; wherein the plurality of multiphoton signals com-prises a first multiphoton signal and a second multiphoton signal of different types. Fast image capture rate multiphoton microscopes for in vivo imaging, as well as photothermolysis methods using the microscopes are also provided.

公开(公告)号：US20120259229A1

公开(公告)日：2012-10-11

申请号：US13516715

申请日：2010-12-17

申请人： Hequn Wang ,  Haishan Zeng ,  Harvey Lui ,  Jianhua Zhao

发明人： Hequn Wang ,  Haishan Zeng ,  Harvey Lui ,  Jianhua Zhao

IPC分类号： A61B6/00

CPC分类号： G01N21/65 ,  A61B5/0075 ,  A61B5/444

摘要： A micro-Raman spectrometer system for use in differentiating tumor lesions from normal skin detects specific characteristics of Raman spectra indicative of cancer. A peak at 899 cm−1 and a higher intensity region in the 1325 cm−1 to 1330 cm−1 range indicate the presence of tumors. The spectrometer system may be applied for skin cancer detection and for mapping the margins of lesions. Cancer detection methods as described herein have achieved diagnostic sensitivity of 95.8% and specificity of 93.8%.

摘要翻译： 用于区分肿瘤病变与正常皮肤的微拉曼光谱仪系统检测指示癌症的拉曼光谱的特征。 在899cm-1处的峰和1325cm-1至1330cm-1范围内的较高强度区域表示存在肿瘤。 光谱仪系统可以应用于皮肤癌检测和用于映射病变的边缘。 本文所述的癌症检测方法具有95.8％的诊断敏感性和93.8％的特异性。