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1.发明授权Process for the preparation of 1,2-dioxetane compounds and novel sulfur-substituted 1,2-dioxetane compounds as intermediates 失效用于制备1,2-二氧杂环丁烷化合物和新型硫取代的1,2-二氧杂环丁烷化合物作为中间体的方法
公开(公告)号：US5936101A
公开(公告)日：1999-08-10
申请号：US981450
申请日：1997-12-22
IPC分类号： C07D321/00 , C07F7/18 , C07F9/655 , C09K11/07 , C07D305/14 , C07D305/00
CPC分类号： C07D321/00 , C07F7/1856 , C07F9/65512
摘要： A process for producing a stable triggerable dioxetane comprising;(a) reacting a vinyl sulfide compound containing a sulfur-substituent SR.sub.4, wherein R.sub.4 is an organic group containing 1 to 20 carbon atoms and optionally heteroatoms, with oxygen and light in the presence of a photosensitizer to form an intermediate sulfur-substituted dioxetane compound; and(b) reacting the sulfur-substituted dioxetane compound with an electrophilic compound E--Y and a hydroxylic compound R.sub.5 OH selected from the group consisting of alcohols, phenols and carboxylic acids or their salts and containing an OR.sub.5 group to replace the SR.sub.4 group of the dioxetane with the OR.sub.5 group.
摘要翻译： PCT No.PCT / US96 / 09248 Sec。 371 1997年12月22日第 102（e）1997年12月22日PCT PCT 1996年6月19日PCT公布。 公开号WO97 / 00869 日期1997年1月9日制备稳定的可触发二氧杂环丁烷的方法， （a）在光敏剂存在下使含有硫取代基SR4的含硫取代基SR4（其中R4是含有1至20个碳原子和任选的杂原子的有机基团）与氧和光反应形成中间体硫取代的二恶丁环化合物 ; 和（b）使硫取代的二恶丁环化合物与亲电子化合物EY和选自醇，酚和羧酸或其盐的羟基化合物R5OH反应，并含有OR 5基团以用二氯乙烷的SR 4基团取代， OR5组。
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2.发明授权1,2-dioxetane compounds with haloalkoxy groups, methods preparation and use 失效具有卤代烷氧基的1,2-二氧杂环丁烷化合物，方法的制备和应用
公开(公告)号：US5773628A
公开(公告)日：1998-06-30
申请号：US339085
申请日：1994-11-14
IPC分类号： C12Q1/42 , C07C43/178 , C07D321/00 , C07F7/18 , C07F9/12 , C07F9/14 , C07F9/655 , C07H15/203 , G01N33/533 , C09K3/00 , C07C43/176 , C12Q1/25
CPC分类号： G01N33/533 , C07C43/1786 , C07D321/00 , C07F7/1856 , C07F9/12 , C07F9/1406 , C07F9/65512 , C07C2103/74
摘要： A chemiluminescent assay method and compositions are described which use a haloalkoxy group-substituted dioxetane which is deprotected by a hydrolytic enzyme to undergo a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with a haloalkoxy group which can be triggered by a reagent to generate light are disclosed. Haloalkoxy group-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.
摘要翻译： 描述了化学发光测定方法和组合物，其使用被水解酶脱保护的卤代烷氧基取代的二氧杂环丁烷进行化学发光反应。 公开了在二恶丁环上用卤代烷氧基取代的化学发光的1,2-二氧环乙烷化合物，其可被试剂触发以产生光。 卤代烷氧基取代的二氧杂环丁烷可用于检测引发剂，包括酶。 该酶可以在免疫测定，DNA探针测定或其它酶结合报告分子的其它测定中单独存在或连接到特异性结合对的成员。
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公开(公告)号：US5883287A
公开(公告)日：1999-03-16
申请号：US981448
申请日：1997-12-22
IPC分类号： C07D521/00 , C07B61/00 , C07C323/11 , C07C323/15 , C07C323/16 , C07F7/18 , C09K11/07 , C07C69/00
CPC分类号： C07C323/16 , C07C2103/74
摘要： Vinyl sulfides bearing protected arlyoxy substituents and a process for their preparation are provided. In the process a vinyl sulfide is formed by coupling a ketone compound and a thioester compound with a low valent titanium reagent to form both carbon-carbon bonds in the same step. The vinyl sulfide compounds are useful as intermediates which are convertible into triggerable sulfur-substituted dioxetanes by addition of oxygen to the double bond. The sulfur-substituted dioxetanes additionally are useful for producing chemiluminescence or for further conversion into triggerable alkoxy, alkenyloxy, alkynloxy, aryloxy, aralkyloxy, or acyloxy-substituted dioxetanes. Triggerable dioxetanes are useful for producing chemiluminescence and in detecting activating agents in assays such as immunoassays and nucleic acid hybridization assays.
摘要翻译： PCT No.PCT / US96 / 09249 Sec。 371 1997年12月22日第 102（e）1997年12月22日PCT PCT 1996年6月19日PCT公布。 出版物WO97 / 00849 日期1997年1月9日提供了具有保护的芳氧基取代基的乙烯基硫化物及其制备方法。 在该方法中，通过在相同步骤中将酮化合物和硫酯化合物与低价钛试剂偶联以形成碳碳键来形成乙烯基硫醚。 乙烯基硫化物化合物可用作通过向双键中加入氧而转化为可触发的硫取代的二氧杂环丁烷的中间体。 硫取代的二氧杂环丁烷另外可用于产生化学发光或进一步转化成可触发的烷氧基，烯氧基，炔氧基，芳氧基，芳烷氧基或酰氧基取代的二氧杂环丁烷。 可触发的二氧杂环丁烷可用于生产化学发光和在诸如免疫测定和核酸杂交测定的测定中检测活化剂。
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公开(公告)号：US08137990B2
公开(公告)日：2012-03-20
申请号：US12541245
申请日：2009-08-14
IPC分类号： G01N33/533 , G01N21/76 , C07D321/00
CPC分类号： C07F5/025 , G01N33/533 , Y10S436/904 , Y10T436/141111 , Y10T436/142222 , Y10T436/206664
摘要： Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O−is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了用于检测过氧化氢源的方法和化合物，其中下式的信号化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。 通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH或Sig-O-的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。
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5.发明申请公开(公告)号：US20080261318A1
公开(公告)日：2008-10-23
申请号：US12144277
申请日：2008-06-23
IPC分类号： G01N33/84
CPC分类号： C07F5/025 , G01N33/533 , Y10S436/904 , Y10T436/141111 , Y10T436/142222 , Y10T436/206664
摘要： Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O−is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了用于检测过氧化氢源的方法和化合物，其中下式的信号化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。 通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH或Sig-O - 的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。
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6.发明授权Processing and synthetic intermediates for preparing N-arylacridancarboxylic acid derivatives 失效用于制备N-芳基吖啶羧酸衍生物的加工和合成中间体公开(公告)号：US06410732B2
公开(公告)日：2002-06-25
申请号：US09770015
申请日：2001-01-25
IPC分类号： C07D21902
CPC分类号： C07D417/04 , C07D219/04 , C07D219/06
摘要： Synthetic processes and intermediates are disclosed for the preparation of N-arylacridancarboxylic acid derivatives. The derivatives are esters, thioesters, amides and sulfonimides. A key feature of the processes is the preparation of N-aryl substituted intermediates by formation of a bond between the nitrogen atom of the acridan ring and a carbon atom of another aromatic or heteroaromatic ring compound. The arylation reaction is catalyzed by a palladium catalyst. The N-arylacridancarboxylic acid derivatives are useful in methods for producing light and in assays for peroxidase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了合成方法和中间体用于制备N-芳基吖啶羧酸衍生物。 衍生物是酯，硫酯，酰胺和磺酰亚胺。 该方法的一个关键特征是通过在吖啶环的氮原子和另一芳族或杂芳族环化合物的碳原子之间形成键而制备N-芳基取代的中间体。 芳基化反应由钯催化剂催化。 N-芳基吖啶羧酸衍生物可用于生产光的方法和用于过氧化物酶和酶抑制剂的测定和使用酶标记的特异性结合对的测定中。
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7.发明授权公开(公告)号：US07704752B2
公开(公告)日：2010-04-27
申请号：US12144277
申请日：2008-06-23
IPC分类号： G01N21/76 , G01N33/533 , C12Q1/28 , C07F5/02 , C07D321/00
CPC分类号： C07F5/025 , G01N33/533 , Y10S436/904 , Y10T436/141111 , Y10T436/142222 , Y10T436/206664
摘要： Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O− is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了用于检测过氧化氢源的方法和化合物，其中下式的信号化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。 通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH或Sig-O-的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。
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公开(公告)号：US20070190526A1
公开(公告)日：2007-08-16
申请号：US11706547
申请日：2007-02-15
申请人： Hashem Akhavan-Tafti , Renuka de Silva , Robert A. Eickholt , Michael E. Mazelis , Wenhuas Xie , Richard S. Handley , Monica A. Bray , Michelle L. Mastronardi , Elizabeth A. O'Conner , Sarada Siripurapu
发明人： Hashem Akhavan-Tafti , Renuka de Silva , Robert A. Eickholt , Michael E. Mazelis , Wenhuas Xie , Richard S. Handley , Monica A. Bray , Michelle L. Mastronardi , Elizabeth A. O'Conner , Sarada Siripurapu
CPC分类号： C12Q1/6806
摘要： Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.
摘要翻译： 公开的方法和材料用于从涉及使用碱性试剂的酸性溶液和固相结合材料的生物样品中快速和简单地提取和分离核酸，特别是RNA，所述固体相结合材料具有将核酸从 生物样品，包括全血，而不首先进行任何初步裂解以破坏细胞或病毒。 不需要或使用用于裂解细胞或病毒的洗涤剂或离液序列物质。 可以使用本发明的方法获得病毒，细菌和哺乳动物基因组RNA。 通过本方法获得的RNA适用于下游过程如RT-PCR。
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公开(公告)号：US08367842B2
公开(公告)日：2013-02-05
申请号：US12836747
申请日：2010-07-15
申请人： Hashem Akhavan-Tafti , Renuka De Silva , Guoping Wang , Robert A. Eickholt , Ravinder K. Gupta , Lakshmi S. Kaanumalle
发明人： Hashem Akhavan-Tafti , Renuka De Silva , Guoping Wang , Robert A. Eickholt , Ravinder K. Gupta , Lakshmi S. Kaanumalle
IPC分类号： C07D277/62
CPC分类号： C07D417/04 , C09B7/00
摘要： The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.
摘要翻译： 本发明提供了基于萤火虫荧光素结构的荧光染料。 这些染料在较短波长下被最佳激发，并具有至少50nm的斯托克斯位移。 本发明的荧光染料可用于制备染料缀合物，其可用于检测样品中的分析物。
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公开(公告)号：US20100055800A1
公开(公告)日：2010-03-04
申请号：US12541245
申请日：2009-08-14
IPC分类号： G01N33/00
CPC分类号： C07F5/025 , G01N33/533 , Y10S436/904 , Y10T436/141111 , Y10T436/142222 , Y10T436/206664
摘要： Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-O−is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了用于检测过氧化氢源的方法和化合物，其中下式的信号化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。 通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH或Sig-O-的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。
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