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1.发明授权1,2-dioxetane compounds with haloalkoxy groups, methods preparation and use 失效具有卤代烷氧基的1,2-二氧杂环丁烷化合物，方法的制备和应用
公开(公告)号：US5773628A
公开(公告)日：1998-06-30
申请号：US339085
申请日：1994-11-14
IPC分类号： C12Q1/42 , C07C43/178 , C07D321/00 , C07F7/18 , C07F9/12 , C07F9/14 , C07F9/655 , C07H15/203 , G01N33/533 , C09K3/00 , C07C43/176 , C12Q1/25
CPC分类号： G01N33/533 , C07C43/1786 , C07D321/00 , C07F7/1856 , C07F9/12 , C07F9/1406 , C07F9/65512 , C07C2103/74
摘要： A chemiluminescent assay method and compositions are described which use a haloalkoxy group-substituted dioxetane which is deprotected by a hydrolytic enzyme to undergo a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with a haloalkoxy group which can be triggered by a reagent to generate light are disclosed. Haloalkoxy group-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.
摘要翻译： 描述了化学发光测定方法和组合物，其使用被水解酶脱保护的卤代烷氧基取代的二氧杂环丁烷进行化学发光反应。 公开了在二恶丁环上用卤代烷氧基取代的化学发光的1,2-二氧环乙烷化合物，其可被试剂触发以产生光。 卤代烷氧基取代的二氧杂环丁烷可用于检测引发剂，包括酶。 该酶可以在免疫测定，DNA探针测定或其它酶结合报告分子的其它测定中单独存在或连接到特异性结合对的成员。
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公开(公告)号：US5883287A
公开(公告)日：1999-03-16
申请号：US981448
申请日：1997-12-22
IPC分类号： C07D521/00 , C07B61/00 , C07C323/11 , C07C323/15 , C07C323/16 , C07F7/18 , C09K11/07 , C07C69/00
CPC分类号： C07C323/16 , C07C2103/74
摘要： Vinyl sulfides bearing protected arlyoxy substituents and a process for their preparation are provided. In the process a vinyl sulfide is formed by coupling a ketone compound and a thioester compound with a low valent titanium reagent to form both carbon-carbon bonds in the same step. The vinyl sulfide compounds are useful as intermediates which are convertible into triggerable sulfur-substituted dioxetanes by addition of oxygen to the double bond. The sulfur-substituted dioxetanes additionally are useful for producing chemiluminescence or for further conversion into triggerable alkoxy, alkenyloxy, alkynloxy, aryloxy, aralkyloxy, or acyloxy-substituted dioxetanes. Triggerable dioxetanes are useful for producing chemiluminescence and in detecting activating agents in assays such as immunoassays and nucleic acid hybridization assays.
摘要翻译： PCT No.PCT / US96 / 09249 Sec。 371 1997年12月22日第 102（e）1997年12月22日PCT PCT 1996年6月19日PCT公布。 出版物WO97 / 00849 日期1997年1月9日提供了具有保护的芳氧基取代基的乙烯基硫化物及其制备方法。 在该方法中，通过在相同步骤中将酮化合物和硫酯化合物与低价钛试剂偶联以形成碳碳键来形成乙烯基硫醚。 乙烯基硫化物化合物可用作通过向双键中加入氧而转化为可触发的硫取代的二氧杂环丁烷的中间体。 硫取代的二氧杂环丁烷另外可用于产生化学发光或进一步转化成可触发的烷氧基，烯氧基，炔氧基，芳氧基，芳烷氧基或酰氧基取代的二氧杂环丁烷。 可触发的二氧杂环丁烷可用于生产化学发光和在诸如免疫测定和核酸杂交测定的测定中检测活化剂。
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3.发明授权Process for the preparation of 1,2-dioxetane compounds and novel sulfur-substituted 1,2-dioxetane compounds as intermediates 失效用于制备1,2-二氧杂环丁烷化合物和新型硫取代的1,2-二氧杂环丁烷化合物作为中间体的方法
公开(公告)号：US5936101A
公开(公告)日：1999-08-10
申请号：US981450
申请日：1997-12-22
IPC分类号： C07D321/00 , C07F7/18 , C07F9/655 , C09K11/07 , C07D305/14 , C07D305/00
CPC分类号： C07D321/00 , C07F7/1856 , C07F9/65512
摘要： A process for producing a stable triggerable dioxetane comprising;(a) reacting a vinyl sulfide compound containing a sulfur-substituent SR.sub.4, wherein R.sub.4 is an organic group containing 1 to 20 carbon atoms and optionally heteroatoms, with oxygen and light in the presence of a photosensitizer to form an intermediate sulfur-substituted dioxetane compound; and(b) reacting the sulfur-substituted dioxetane compound with an electrophilic compound E--Y and a hydroxylic compound R.sub.5 OH selected from the group consisting of alcohols, phenols and carboxylic acids or their salts and containing an OR.sub.5 group to replace the SR.sub.4 group of the dioxetane with the OR.sub.5 group.
摘要翻译： PCT No.PCT / US96 / 09248 Sec。 371 1997年12月22日第 102（e）1997年12月22日PCT PCT 1996年6月19日PCT公布。 公开号WO97 / 00869 日期1997年1月9日制备稳定的可触发二氧杂环丁烷的方法， （a）在光敏剂存在下使含有硫取代基SR4的含硫取代基SR4（其中R4是含有1至20个碳原子和任选的杂原子的有机基团）与氧和光反应形成中间体硫取代的二恶丁环化合物 ; 和（b）使硫取代的二恶丁环化合物与亲电子化合物EY和选自醇，酚和羧酸或其盐的羟基化合物R5OH反应，并含有OR 5基团以用二氯乙烷的SR 4基团取代， OR5组。
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4.发明授权Water soluble tri-substituted 1, 2-dioxetane compounds having increased storage stability, synthetic processes and intermediates 失效具有增加储存稳定性的水溶性三取代1,2-二氧环乙烷化合物，合成方法和中间体公开(公告)号：US06410751B1
公开(公告)日：2002-06-25
申请号：US09839472
申请日：2001-04-20
IPC分类号： C07F909
CPC分类号： G01N33/582 , C07C69/734 , C07C2603/74 , C07D321/00 , C07F9/12 , C07F9/65512 , G01N33/5308 , G01N33/54326
摘要： Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility and storage stability are provided as well as synthetic processes and intermediates used in their preparation. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure and an additional fluorine atom or lower alkyl group provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. These dioxetanes display substantially improved stability on storage. Compositions comprising these dioxetanes, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.
摘要翻译： 提供了具有改善的水溶性和储存稳定性的稳定的，酶促触发的化学发光1,2-二氧杂环丁烷，以及用于其制备的合成方法和中间体。 二氧杂环丁烷进一步被设置在二氧杂环丁烷结构上的水溶性基团和另外的氟原子或低级烷基取代，当用于胶囊化学分析系统的测定中时，通过消除试剂残留的问题而提供优异的性能。 这些二氧杂环丁烷在储存时显示出显着提高的稳定性。 还提供了包含这些二氧杂环丁烷的组合物，非聚合阳离子表面活性剂增强剂和任选的荧光剂，用于提供增强的化学发光。
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5.发明授权Water soluble tri-substituted 1,2-dioxetane compounds having increased storage stability, synthetic processes and intermediates 失效具有增加储存稳定性，合成方法和中间体的水溶性三取代1,2-二氧杂环丁烷化合物
公开(公告)号：US6036892A
公开(公告)日：2000-03-14
申请号：US101331
申请日：1998-07-07
IPC分类号： G01N33/535 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/655 , C09K11/06 , C09K11/07 , G01N33/53 , G01N33/543 , G01N33/58 , C09K3/00 , C07C69/76 , C07F9/02 , C07F9/06 , C07H17/08
CPC分类号： C07C69/00 , C07D321/00 , C07F9/12 , C07F9/65512 , G01N33/5308 , G01N33/54326 , G01N33/582 , C07C2103/00
摘要： Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility and storage stability are provided as well as synthetic processes and intermediates used in their preparation. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure and an additional fluorine atom or lower alkyl group provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. These dioxetanes display substantially improved stability on storage. Compositions comprising these dioxetanes, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.
摘要翻译： PCT No.PCT / US97 / 19618 Sec。 371日期：1998年7月7日 102（e）日期1998年7月7日PCT 1997年11月7日PCT公布。 第WO98 / 19989号公报 日期1998年5月14日提供稳定的酶促触发的化学发光1,2-二氧杂环丁烷，具有改善的水溶性和储存稳定性，以及用于其制备的合成方法和中间体。 二氧杂环丁烷进一步被设置在二氧杂环丁烷结构上的水溶性基团和另外的氟原子或低级烷基取代，当用于胶囊化学分析系统的分析中时，通过消除试剂残留的问题而提供优异的性能。 这些二氧杂环丁烷在储存时显示出显着提高的稳定性。 还提供了包含这些二氧杂环丁烷的组合物，非聚合阳离子表面活性剂增强剂和任选的荧光剂，用于提供增强的化学发光。
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公开(公告)号：US5721370A
公开(公告)日：1998-02-24
申请号：US748107
申请日：1996-11-08
IPC分类号： G01N33/535 , C07C69/00 , C07D305/08 , C07D321/00 , C07F9/12 , C07F9/655 , C09K11/06 , C09K11/07 , G01N21/76 , G01N33/53 , G01N33/543 , G01N33/58 , C07F9/28
CPC分类号： C07C69/734 , C07C67/343 , C07D321/00 , C07F9/12 , C07F9/65512 , G01N33/5308 , G01N33/54326 , G01N33/582 , C07C2103/74
摘要： Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility and storage stability are provided. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure and an additional fluorine atom or lower alkyl group provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. These dioxetanes display substantially improved stability on storage. Compositions comprising these dioxetanes, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.
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公开(公告)号：US06891057B2
公开(公告)日：2005-05-10
申请号：US10754750
申请日：2004-01-09
IPC分类号： C07D219/00 , C07D219/02 , C07D221/08 , C07F7/18 , C07F9/113 , C07F9/64 , C07F9/6553 , C09K11/06 , G01N33/58 , C07F9/02
CPC分类号： C07F9/113 , C07D219/02 , C07D221/08 , C07F7/1804 , C07F9/64 , C07F9/655372 , C09K11/06 , G01N33/582
摘要： Novel compounds comprising a C—C double bond substituted at one carbon with two oxygen or sulfur atom-containing groups are disclosed. The compounds are useful in methods and compositions for generating chemiluminescence by reaction with a peroxidase enzyme and a peroxide. The chemiluminescence thus produced can be used as a detectable signal in assays for peroxidase enzymes and in assays employing enzyme-labeled specific binding pairs.
摘要翻译： 公开了包含在一个碳上被两个含氧或含硫原子的基团取代的C-C双键的新型化合物。 该化合物可用于通过与过氧化物酶和过氧化物反应产生化学发光的方法和组合物。 由此产生的化学发光可以用作过氧化物酶测定中的可检测信号，并且在使用酶标记的特异性结合对的测定中可以使用。
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8.发明授权
公开(公告)号：US6139782A
公开(公告)日：2000-10-31
申请号：US358004
申请日：1999-07-21
IPC分类号： G01N33/532 , C07D219/00 , C07F1/04 , C07F9/572 , C07F9/576 , C07F9/64 , C07F9/6541 , C09K3/00 , C09K11/07 , C12N11/02 , C12N15/09 , C12Q1/42 , G01N21/78 , G01N27/447 , G01N33/53 , G01N33/535
CPC分类号： C07F9/6541 , C07F9/5765 , C07F9/64 , C12Q1/42 , C12Q2334/00 , Y10S435/968
摘要： Novel heterocyclic compounds which generate chemiluminescence on reaction with a phosphatase enzyme are provided as well as a process for their preparation and intermediates useful therein. The compounds comprise a nitrogen, oxygen or sulfur-containing heterocyclic ring system bearing an exocyclic carbon-carbon double bond. The double bond is further substituted at the distal carbon with a phosphate group and an oxygen or sulfur atom-containing group.Novel compositions further comprising a cationic aromatic compound (CAC) in addition to the heterocyclic phosphate compound are provided. The addition of the CAC in the composition greatly increases the production of chemiluminescence and provides improved detection sensitivity. Compositions further comprising an anionic surfactant and a non-ionic surfactant provide additional improvements in detection sensitivity. The novel chemiluminescent compounds and compositions are useful in methods for producing light and in assays for phosphatase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.
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9.发明授权
公开(公告)号：US6090571A
公开(公告)日：2000-07-18
申请号：US358002
申请日：1999-07-21
IPC分类号： G01N33/532 , C07D219/00 , C07F1/04 , C07F9/572 , C07F9/576 , C07F9/64 , C07F9/6541 , C09K3/00 , C09K11/07 , C12N11/02 , C12N15/09 , C12Q1/42 , G01N21/78 , G01N27/447 , G01N33/53 , G01N33/535
CPC分类号： C07F9/6541 , C07F9/5765 , C07F9/64 , C12Q1/42 , C12Q2334/00 , Y10S435/968
摘要： Novel heterocyclic compounds which generate chemiluminescence on reaction with a phosphatase enzyme are provided as well as a process for their preparation and intermediates useful therein. The compounds comprise a nitrogen, oxygen or sulfur-containing heterocyclic ring system bearing an exocyclic carbon-carbon double bond. The double bond is further substituted at the distal carbon with a phosphate group and an oxygen or sulfur atom-containing group.Novel compositions further comprising a cationic aromatic compound (CAC) in addition to the heterocyclic phosphate compound are provided. The addition of the CAC in the composition greatly increases the production of chemiluminescence and provides improved detection sensitivity. Compositions further comprising an anionic surfactant and a non-ionic surfactant provide additional improvements in detection sensitivity. The novel chemiluminescent compounds and compositions are useful in methods for producing light and in assays for phosphatase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.
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10.发明授权Aryl N-alkylacridanthiocarboxylate derivatives useful for chemiluminescent detection 失效可用于化学发光检测的N-烷基酰基羧酸芳基酯衍生物
公开(公告)号：US5523212A
公开(公告)日：1996-06-04
申请号：US228290
申请日：1994-04-15
IPC分类号： G01N21/78 , C07D219/02 , C07D219/04 , C07D219/06 , C09B15/00 , C09K11/07 , C12Q1/28 , C12Q1/68 , G01N21/76 , G01N33/532 , G01N33/535 , G01N33/566 , C07H5/04 , C12Q1/00 , C12Q1/26
CPC分类号： C07D219/06 , C07D219/02 , C07D219/04 , C12Q1/28 , Y10S435/81 , Y10S435/968
摘要： Aryl N-alkylacridanthiocarboxylate compounds which produce chemiluminescence. The compounds produce light with peroxide and peroxidase. The compounds are used as a substrate in assays for various analytes.
摘要翻译： 产生化学发光的N-烷基酰基羧酸芳酯化合物。 该化合物用过氧化物和过氧化物酶产生光。 该化合物用作各种分析物测定中的底物。
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