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1.发明授权Nucleosides, nucleotides and oligonucleotides containing enzymatically cleavable protecting groups 失效含有酶切割保护基团的核苷，核苷酸和寡核苷酸
公开(公告)号：US5677441A
公开(公告)日：1997-10-14
申请号：US326490
申请日：1994-10-20
申请人： Herbert Waldman , Armin Reidel , Axel Heuser , Klaus Muehlegger , Herbert Von Der Eltz , Christian Birkner
发明人： Herbert Waldman , Armin Reidel , Axel Heuser , Klaus Muehlegger , Herbert Von Der Eltz , Christian Birkner
IPC分类号： C12Q1/68 , C07H19/04 , C07H19/073 , C07H19/173 , C07H21/00 , C07H21/04 , C12Q1/70 , C07H19/06 , C07H19/16
摘要： Process for the production of oligonucleotides of formula II, in which the exocyclic amino groups of the bases adenine, guanine, cytosine, 7-deazaadenine and 7-deazaguanine carry N-phenylacetyl groups, are used for oligonucleotide synthesis, wherein in a first step a starting nucleotide is bound to a solid carrier, subsequently the desired oligonucleotide is synthesized by stepwise coupling with appropriately activated further monomeric nucleotide building blocks of the general formula I with the above-mentioned meanings, if desired, trivalent phosphorus is oxidized to pentavalent phosphorus during and after the synthesis, the oligonucleotide is cleaved from the carrier and the 5' protecting groups are cleaved off. The phenylacetyl functional groups that protect exocyclic NH.sub.2 groups of the bases can be cleaved off in a mild way with penicillin amidohydrolase (EC 3.5.1.11).
摘要翻译： 用于生产式II寡核苷酸的方法，其中碱基腺嘌呤，鸟嘌呤，胞嘧啶，7-脱氮鸟嘌呤和7-脱氮鸟嘌呤的环外氨基携带N-苯乙酰基用于寡核苷酸合成，其中在第一步中 起始核苷酸与固体载体结合，随后所需的寡核苷酸通过与适当活化的具有上述含义的通式I的其它单体核苷酸结构单元逐步偶联来合成，如果需要，三价磷被氧化成五价磷， 合成后，将寡核苷酸从载体上切下，5'保护基被切掉。 保护碱基的环外NH2基团的苯乙酰基官能团可以用青霉素酰胺水解酶（EC 3.5.1.11）以温和的方式裂解。
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2.发明授权
公开(公告)号：US5756704A
公开(公告)日：1998-05-26
申请号：US844127
申请日：1997-04-18
申请人： Herbert Waldman , Armin Reidel , Axel Heuser , Klaus Muehlegger , Herbert Von Der Eltz , Christian Birkner
发明人： Herbert Waldman , Armin Reidel , Axel Heuser , Klaus Muehlegger , Herbert Von Der Eltz , Christian Birkner
IPC分类号： C12Q1/68 , C07H19/04 , C07H19/073 , C07H19/173 , C07H21/00 , C07H21/04 , C12Q1/70 , C07H1/00 , C07H1/02
摘要： Process for the production of oligonucleotides of formula II, in which the exocyclic amino groups of the bases adenine, guanine, cytosine, 7-deazaadenine and 7-deazaguanine carry N-phenylacetyl groups, are used for oligonucleotide synthesis, wherein in a first step a starting nucleotide is bound to a solid carrier, subsequently the desired oligonucleotide is synthesized by stepwise coupling with appropriately activated further monomeric nucleotide building blocks of the general formula I with the above-mentioned meanings, if desired, trivalent phosphorus is oxidized to pentavalent phosphorus during and after the synthesis, the oligonucleotide is cleaved from the carrier and the 5' protecting groups are cleaved off. The phenylacetyl functional groups that protect exocyclic NH.sub.2 groups of the bases can be cleaved off in a mild way with penicillin amidohydrolase (EC 3.5.1.11).
摘要翻译： 用于生产式II寡核苷酸的方法，其中碱基腺嘌呤，鸟嘌呤，胞嘧啶，7-脱氮鸟嘌呤和7-脱氮鸟嘌呤的环外氨基携带N-苯乙酰基用于寡核苷酸合成，其中在第一步中 起始核苷酸与固体载体结合，随后所需的寡核苷酸通过与适当活化的具有上述含义的通式I的其它单体核苷酸结构单元逐步偶联来合成，如果需要，三价磷被氧化成五价磷， 合成后，将寡核苷酸从载体上切下，5'保护基被切掉。 保护碱基的环外NH2基团的苯乙酰基官能团可以用青霉素酰胺水解酶（EC 3.5.1.11）以温和的方式裂解。
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3.发明授权Nucleotides labelled with an infrared dye and their use in nucleic acid detection 失效用红外染料标记的核苷酸及其在核酸检测中的用途公开(公告)号：US06573374B1
公开(公告)日：2003-06-03
申请号：US08411761
申请日：1995-03-28
IPC分类号： C07H1904
CPC分类号： C07H19/10 , C07F9/5728 , C07H19/20 , C07H21/00
摘要： Nucleoside-5′-triphosphates and phosphoramidites which carry a residue absorbing in the long wavelength region, preferably a carbocyanine group of the general formula (I), on the base portion or on the phosphorus atom in which R1 and R2 each denote hydrogen or together form a phenyl residue; R3 denotes hydrogen if linkage with the nucleotide is via the R4 position or it denotes a —NHCS— group if linkage with the nucleotide is via the R3 position; both R4 and R5, or R5, alone denote an alkylsulfonyl group with n being a number from 3 to 5 or R4 represents a —NHCS— group with n being a number from 3 to 8, as well as the use of the compounds to label, detect and sequence nucleic acids.
摘要翻译： 核苷-5'-三磷酸和亚磷酰胺，其在碱性部分或磷原子上具有在长波长区域吸收的残基，优选为通式（I）的碳菁基团，其中R1和R2各自表示氢或一起形成 苯基残基; 如果与核苷酸的连接通过R4位置，R3表示氢，如果与核苷酸的连接通过R3位置，则表示-NHCS-基团; R4和R5或R5独立地表示烷基磺酰基，n为3至5的数，或R4表示-NHCS-基，n为3至8的数，以及该化合物用于标记 ，检测和序列核酸。
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公开(公告)号：US07501240B2
公开(公告)日：2009-03-10
申请号：US10573215
申请日：2004-12-01
CPC分类号： C07H21/00 , C12Q1/6827 , C12Q2527/125 , C12Q2523/125
摘要： The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.
摘要翻译： 本发明涉及检测核酸中的甲基化胞嘧啶，其中将亚硫酸氢胍用于制备含有胍离子和亚硫酸根离子的溶液以及随后的核酸修饰。 由此，将非甲基化胞嘧啶转化为尿嘧啶。 本发明还公开了用于执行本发明方法的套件。
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公开(公告)号：US20080319182A1
公开(公告)日：2008-12-25
申请号：US12105556
申请日：2008-04-18
CPC分类号： C12N15/1006
摘要： Disclosed are water-soluble ionic liquids suitable for promoting adsorption of nucleic acids to a solid phase. The use thereof, particularly methods for the isolation of nucleic acids from an aqueous solution, as well as kits for performing those methods are disclosed.
摘要翻译： 公开了适用于促进核酸吸附到固相的水溶性离子液体。 公开了其用途，特别是用于从水溶液中分离核酸的方法，以及用于实施这些方法的试剂盒。
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公开(公告)号：US20070190530A1
公开(公告)日：2007-08-16
申请号：US10573215
申请日：2004-12-01
CPC分类号： C07H21/00 , C12Q1/6827 , C12Q2527/125 , C12Q2523/125
摘要： The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.
摘要翻译： 本发明涉及检测核酸中的甲基化胞嘧啶，其中将亚硫酸氢胍用于制备含有胍离子和亚硫酸根离子的溶液，随后改变核酸。 由此，将非甲基化胞嘧啶转化为尿嘧啶。 本发明还公开了用于执行本发明方法的套件。
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公开(公告)号：US08101744B2
公开(公告)日：2012-01-24
申请号：US12105556
申请日：2008-04-18
IPC分类号： C07H21/00
CPC分类号： C12N15/1006
摘要： Disclosed are water-soluble ionic liquids suitable for promoting adsorption of nucleic acids to a solid phase. The use thereof, particularly methods for the isolation of nucleic acids from an aqueous solution, as well as kits for performing those methods are disclosed.
摘要翻译： 公开了适用于促进核酸吸附到固相的水溶性离子液体。 公开了其用途，特别是用于从水溶液中分离核酸的方法，以及用于实施这些方法的试剂盒。
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公开(公告)号：US20110053251A1
公开(公告)日：2011-03-03
申请号：US12855803
申请日：2010-08-13
摘要： The invention provides a composition containing an aqueous liquid phase, bacterial cells, and an ionic compound dissolved in the liquid phase. The ionic compound is selected from the group consisting of 1-butyl-3-methyl-imidazolium-thiocyanate, 1-butyl-3-methyl-imidazolium-2(2-methoxy-ethoxy)ethylsulfate, 1-methyl-1-[4-(3-methyl-3H-imidazol-1-ium)-but-1-yl]-3H-imidazolium-di(toluylsulfate), and 1-butyl-3-methyl-imidazolium-octylsulfate. The compositions of the invention are advantageously used for preparing lysates of biological cells, particularly bacterial cells.
摘要翻译： 本发明提供一种含有水相液相，细菌细胞和溶于液相的离子化合物的组合物。 离子化合物选自1-丁基-3-甲基 - 咪唑鎓 - 硫氰酸酯，1-丁基-3-甲基 - 咪唑鎓-2（2-甲氧基 - 乙氧基）乙基硫酸酯，1-甲基-1- [4 - （3-甲基-3H-咪唑-1-基） - 丁-1-基] -3H-咪唑鎓 - 二（甲苯基硫酸盐）和1-丁基-3-甲基 - 咪唑鎓辛基硫酸盐。 本发明的组合物有利地用于制备生物细胞，特别是细菌细胞的裂解物。
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9.发明授权Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification 有权苯并咪唑鎓化合物和苯并咪唑化合物的盐用于核酸扩增公开(公告)号：US07781165B2
公开(公告)日：2010-08-24
申请号：US11581735
申请日：2006-10-16
IPC分类号： C12Q1/68 , C12P19/34 , C07D235/00
CPC分类号： C12Q1/6844 , C12Q2527/125
摘要： The present invention is directed to the use of a benzimidazolium compound comprising a side chain at at least one of its N-residues, said chain being either a Cn-alkyl or a substituted Cn-alkyl, characterized in that preferably n is ≧3, as an additive for a nucleic acid amplification reaction.
摘要翻译： 本发明涉及在其N-残基中的至少一个上含有侧链的苯并咪唑化合物的用途，所述链是C 1 - 烷基或取代的C n - 烷基，其特征在于n优选≥3， 作为核酸扩增反应的添加剂。
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公开(公告)号：US20070111234A1
公开(公告)日：2007-05-17
申请号：US11514777
申请日：2006-09-01
CPC分类号： C12Q1/6806 , C12Q2565/627 , C12Q2561/113
摘要： The present invention provides a method, a kit and a system for the analysis of samples with respect to the presence of biological DNA based on real-time polymerase chain reaction (PCR) in combination with an analyzing step. The method comprises the steps of performing a universal real-time PCR to amplify biological DNA in the sample, and analyzing the amplified biological DNA using a mass spectrometer (MS).
摘要翻译： 本发明提供了一种与分析步骤相结合的基于实时聚合酶链式反应（PCR）的生物DNA存在的样品分析方法，试剂盒和系统。 该方法包括以下步骤：进行通用实时PCR以扩增样品中的生物DNA，并使用质谱仪（MS）分析扩增的生物DNA。
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