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公开(公告)号：US5804423A
公开(公告)日：1998-09-08
申请号：US594808
申请日：1996-01-31
CPC分类号： C12P7/58
摘要： A method is disclosed for preparing 5-ketogluconate, which comprises the steps of: (a) genetically modifying a microorganism capable of microbiologically producing 5-ketogluconate to increase gluconate NADP.sup.+ -5-oxidoreductase gene expression of said microorganism; (b) culturing said microorganism in a medium to produce 5-ketogluconate; and (c) recovering 5-ketogluconate from said medium.
摘要翻译： 公开了一种制备5-酮葡萄糖酸盐的方法，其包括以下步骤：（a）遗传修饰能够微生物产生5-酮葡萄糖酸盐的微生物，以增加所述微生物的葡萄糖酸化NADP + 5-氧化还原酶基因表达; （b）在培养基中培养所述微生物以产生5-酮葡萄糖酸盐; 和（c）从所述培养基中回收5-酮葡萄糖酸盐。
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2.发明授权Process for obtaining sorbitol and gluconic acid by fermentation, and cell material suitable for this purpose 失效通过发酵获得山梨糖醇和葡萄糖酸的方法，以及适用于该目的的细胞材料
公开(公告)号：US5017485A
公开(公告)日：1991-05-21
申请号：US448334
申请日：1989-12-11
CPC分类号： C12P7/18 , C12P7/58 , Y10S435/822
摘要： A process is disclosed for obtaining sorbitol and gluconic acid, or gluconate, by fermentation in aqueous glucose/fructose mixtures. Zymomonas mobilis cells are used which have been permeabilized by the freeze technique and which have preferably been obtained by freezing cell centrifugates of pH 6 to 7 at about -20.degree. C., and thawing at room temperature. Cell concentrations of 20 to 60 g of cell dry matter of permeabilized cells/l are preferably used for the fermentation. By using cells which have been permeabilized by freezing and thawing, a considerably higher conversion rate of glucose/fructose into sorbitol and gluconic acid, or gluconate, is achieved.
摘要翻译： 公开了通过在水性葡萄糖/果糖混合物中发酵获得山梨糖醇和葡萄糖酸或葡萄糖酸盐的方法。 使用通过冷冻技术透化的运动发酵单胞菌细胞，其优选通过在约-20℃下冷冻pH6-7的细胞离心液并在室温下解冻获得。 细胞浓度为20〜60g的透化细胞/ l细胞干物质优选用于发酵。 通过使用通过冷冻和解冻而透化的细胞，实现了相当高的葡萄糖/果糖转化为山梨醇和葡糖酸或葡萄糖酸的转化率。
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3.发明申请Nucleotide sequence coding for a mannitol-2 dehydrogenase and method for the production of d-mannitol 审中-公开编码甘露糖醇2脱氢酶的核苷酸序列和D-甘露糖醇的制备方法公开(公告)号：US20050239180A1
公开(公告)日：2005-10-27
申请号：US10514031
申请日：2003-05-06
申请人： Hermann Sahm , Gerald Hahn , Stephanie Bringer-Meyer , Bjorn Kaup , Claudia Hemmerling , Martin Walter , Dieter Wullbrandt
发明人： Hermann Sahm , Gerald Hahn , Stephanie Bringer-Meyer , Bjorn Kaup , Claudia Hemmerling , Martin Walter , Dieter Wullbrandt
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/04 , C12P7/18 , C07H21/04 , C12N9/02 , C12N15/74
CPC分类号： C12N9/0006 , C12P7/18
摘要： The invention relates to a nucleotide sequence coding for the mannitol 2-dehydrogenase and a method for producing D-mannitol. Previously known biocatalytic methods for producing D-mannitol yield only small production rates due to the small number of specific activities of mannitol 2-dehydrogenases used for transforming D-fructose into D-mannitol. D-mannitol production can be improved by supplying a nucleotide sequence which codes for a mannitol 2-dehydrogenase having a higher specific activity. Mannitol production can be increased in a particular manner by creating a regeneration system for reduction equivalents by introducing and/or strengthening a formiate dehydrogenase in a microorganism.
摘要翻译： 本发明涉及编码甘露醇2-脱氢酶的核苷酸序列和D-甘露糖醇的制造方法。 由于用于将D-果糖转化为D-甘露糖醇的甘露醇2-脱氢酶的比活性较少，以前已知的用于生产D-甘露糖的生物催化方法产生的生产速率较小。 通过提供编码具有较高比活性的甘露糖醇2-脱氢酶的核苷酸序列可以改善D-甘露糖醇的产生。 通过在微生物中引入和/或强化甲酸脱氢酶，通过产生用于还原当量的再生系统，可以以特定方式增加甘露糖醇生产。
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公开(公告)号：US5763656A
公开(公告)日：1998-06-09
申请号：US849006
申请日：1997-05-08
申请人： Ralf Klasen , Hermann Sahm , Ingo Matzerath , Wolfgang Klaui
发明人： Ralf Klasen , Hermann Sahm , Ingo Matzerath , Wolfgang Klaui
IPC分类号： C07C51/16 , C07C59/255 , C07C51/245
CPC分类号： C07C51/16
摘要： A method of preparing tartaric acid in which 5-ketogluconate is oxidized in a carbonate buffer at an alkaline pH preferably in the range of 8 to 10 using vanadate.
摘要翻译： PCT No.PCT / DE95 / 01568 Sec。 371日期：1997年5月8日 102（e）日期1997年5月8日PCT提交1995年11月8日PCT公布。 公开号WO96 / 15095 日期1996年5月23日一种制备酒石酸的方法，其中使用钒酸盐的碱性pH优选在8至10范围内，在碳酸盐缓冲液中氧化5-酮葡萄糖酸盐。
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5.发明授权Nucleotide sequences of coryneform bacteria coded for proteins participating in L-serine metabolism and method for microbial production of L-serine 失效编码参与L-丝氨酸代谢的蛋白质的棒状细菌的核苷酸序列和用于L-丝氨酸的微生物生产的方法公开(公告)号：US08119374B2
公开(公告)日：2012-02-21
申请号：US12136190
申请日：2008-06-10
摘要： This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.
摘要翻译： 本发明涉及编码与L-丝氨酸脱水酶活性降低和关闭的L-丝氨酸代谢相关的蛋白质的棒状细菌的核苷酸序列。 本发明还涉及用于生产L-丝氨酸的方法中使用的微生物。
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6.发明申请L-LYSINE-PRODUCING CORYNEBACTERIA AND PROCESS FOR THE PREPARATION OF L-LYSINE 有权生产L-赖氨酸的L-赖氨酸和L-赖氨酸的制备方法公开(公告)号：US20100261233A1
公开(公告)日：2010-10-14
申请号：US12249092
申请日：2008-10-10
申请人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
IPC分类号： C12P13/08
摘要： L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
摘要翻译： 具有增强的lysE基因（赖氨酸出口载体基因）的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因）的lys附加基因，lysC基因（天冬氨酸激酶基因），dapB基因 （二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）增强，特别是过表达，以及制备L-赖氨酸的方法。
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7.发明申请公开(公告)号：US20080153139A1
公开(公告)日：2008-06-26
申请号：US11663984
申请日：2006-04-06
申请人： Jan Marienhagen , Lothar Eggeling , Hermann Sahm
发明人： Jan Marienhagen , Lothar Eggeling , Hermann Sahm
CPC分类号： C12P13/08 , C12N9/1096
摘要： The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a coryneform bacterium, especially Corynebacteriuim glutamicum. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.
摘要翻译： 本发明涉及L-缬氨酸和合适微生物的生产方法。 本发明的方法的特征在于优选增强棒状细菌，特别是谷氨酸棒杆菌（Corynebacteriuim glutamicum）的转氨酶C活性。 如此修饰的生物体在L-缬氨酸中的产率高于非改性生物体的35.8％。
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8.发明申请L-LYSINE-PRODUCING CORYNEBACTERIA AND PROCESS FOR THE PREPARATION OF L-LYSINE 有权生产L-赖氨酸的L-赖氨酸和L-赖氨酸的制备方法公开(公告)号：US20070054380A1
公开(公告)日：2007-03-08
申请号：US11425091
申请日：2006-06-19
申请人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。
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9.发明授权L-Lysine-producing corynebacteria and process for the preparation of L-lysine 有权L-赖氨酸生产性棒状杆菌和L-赖氨酸的制备方法公开(公告)号：US07094584B2
公开(公告)日：2006-08-22
申请号：US10804120
申请日：2004-03-19
申请人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Möckel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Möckel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
IPC分类号： C12P13/08
摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。
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10.发明授权Nucleotide sequences coding for the thrE gene and process for the enzymatic production of L-threonine using coryneform bacteria 失效编码thrE基因的核苷酸序列和使用棒状细菌酶促生产L-苏氨酸的方法公开(公告)号：US06913909B2
公开(公告)日：2005-07-05
申请号：US09963521
申请日：2001-09-27
申请人： Petra Ziegler , Lothar Eggeling , Hermann Sahm , Georg Thierbach
发明人： Petra Ziegler , Lothar Eggeling , Hermann Sahm , Georg Thierbach
IPC分类号： C12N15/09 , C07K14/34 , C12N1/21 , C12N15/01 , C12N15/31 , C12N15/77 , C12P13/08 , C12R1/15 , C12N1/20 , C12N15/00 , C12P21/06 , C12Q1/48
摘要： The invention relates to preferably recombinant DNA derived from Corynebacterium and replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine, which is characterised in that the following steps are carried out: a) Fermentation of microorganisms in which at least the thrE gene is amplified (overexpressed), optionally in combination with further genes, b) Enrichment of the L-threonine in the medium or in the cells of the microorganisms, and c) Isolation of the L-threonine.
摘要翻译： 本发明涉及优选来源于棒状杆菌的重组DNA，并可在包含编码thrE基因的至少一个核苷酸序列的棒状细菌中复制，以及L-苏氨酸的制备方法，其特征在于以下步骤为 进行：a）至少将thrE基因扩增（过表达）的微生物发酵，任选与其它基因组合，b）在培养基中或微生物细胞中富集L-苏氨酸，和c） L-苏氨酸的分离。
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