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1.发明授权Process for the production of D-.alpha.-amino acids by hydrolysis of the corresponding N-carbamyl derivative 失效通过水解相应的N-氨基甲酰基衍生物生产D-氨基酸的方法
公开(公告)号：US5902736A
公开(公告)日：1999-05-11
申请号：US244657
申请日：1994-06-06
申请人： Hideaki Yamada , Sakayu Shimizu , Yasuhiro Ikenaka , Kazuyoshi Yajima , Yukio Yamada , Hirokazu Nanba , Masayuki Takano , Satomi Takahashi
发明人： Hideaki Yamada , Sakayu Shimizu , Yasuhiro Ikenaka , Kazuyoshi Yajima , Yukio Yamada , Hirokazu Nanba , Masayuki Takano , Satomi Takahashi
CPC分类号： C12P13/04 , C12P41/009 , Y10S435/874 , Y10S435/911
摘要： In a process for the production of a D-.alpha.-amino acid, in which an N-carbamyl-D-.alpha.-amino acid corresponding to the general formula: ##STR1## wherein R represents phenyl, hydroxy-substituted phenyl, substituted or unsubstituted alkyl, or thienyl, is converted by a microbial enzyme in an aqueous medium to a D-.alpha.-amino acid corresponding to the general formula: ##STR2## wherein R is the same as defined above, decarbamylase produced by a microorganism of the genus Comamonas, Blastobacter, Alcaligenes, Sporosarcina, Rhizobium, Bradyrhizobium or Arthrobacter is used as the enzyme converting the N-carbamyl-D-.alpha.-amino acid to the D-.alpha.-amino acid.The conversion of the N-carbamyl-D-.alpha.-amino acids to the D-.alpha.-amino acids is carried out in a neutral to alkaline pH range.
摘要翻译： PCT No.PCT / JP93 / 01408 Sec。 371日期：1994年6月6日 102（e）日期1994年6月6日PCT提交1993年10月1日PCT公布。 公开号WO94 / 08030 日期1994年4月14日在制备D-氨基酸的方法中，其中N-氨基甲酰基-D-α-氨基酸对应于通式：其中R表示苯基，羟基取代的苯基，取代的 或未取代的烷基或噻吩基通过微生物酶在水性介质中转化成对应于以下通式的D-α-氨基酸：其中R与上述定义相同，由Comamonas属的微生物产生的脱氨基甲酰酶， 使用喷杆菌属，产碱杆菌属，孢子体菌属，根瘤菌属，Bradyrhizobium或节杆菌作为将N-氨基甲酰基-D-α-氨基酸转化为D-氨基酸的酶。 N-氨基甲酰基-D-α-氨基酸向D-α-氨基酸的转化在中性至碱性pH范围内进行。
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公开(公告)号：US5863785A
公开(公告)日：1999-01-26
申请号：US479639
申请日：1995-06-07
申请人： Hideaki Yamada , Sakayu Shimizu , Yasuhiro Ikenaka , Kazuyoshi Yajima , Yukio Yamada , Hirokazu Nanba , Masayuki Takano , Satomi Takahashi
发明人： Hideaki Yamada , Sakayu Shimizu , Yasuhiro Ikenaka , Kazuyoshi Yajima , Yukio Yamada , Hirokazu Nanba , Masayuki Takano , Satomi Takahashi
CPC分类号： C12P13/04 , C12P41/009 , Y10S435/874 , Y10S435/911
摘要： In a process for the production of a D-.alpha.-amino acid, in which an N-carbamyl-D-.alpha.-amino acid corresponding to the general formula: ##STR1## wherein R represents phenyl, hydroxy-substituted phenyl, substituted or unsubstituted alkyl, or thienyl, is converted by a microbial enzyme in an aqueous medium to a D-.alpha.-amino acid corresponding to the general formula: ##STR2## wherein R is the same as defined above, decarbamylase produced by a microorganism of the genus Comamonas, Blastobacter, Alcaligenes, Sporosarcina, Rhizobium, Bradyrhizobium or Arthrobacter is used as the enzyme converting the N-carbamyl-D-.alpha.-amino acid to the D-.alpha.-amino acid.The conversion of the N-carbamyl-D-.alpha.-amino acids to the D-.alpha.-amino acids is carried out in a neutral to alkaline pH range.
摘要翻译： 在制备D-α-氨基酸的方法中，其中相应于通式为：苯基，取代或未取代的烷基或噻吩基的N-氨基甲酰基-D-α-氨基酸被转化 通过微生物酶在水性介质中与对应于以下通式的D-α-氨基酸反应：其中R与上述定义相同，由Comamonas属，Blastobacter，Alcaligenes，Sporosarcina属的微生物产生的脱氨基甲酰酶， 使用根瘤菌，Bradyrhizobium或节杆菌作为将N-氨基甲酰基-D-α-氨基酸转化为D-α-氨基酸的酶。 N-氨基甲酰基-D-α-氨基酸向D-α-氨基酸的转化在中性至碱性pH范围内进行。
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公开(公告)号：US06794171B2
公开(公告)日：2004-09-21
申请号：US10133372
申请日：2002-04-29
IPC分类号： C12N914
CPC分类号： C12N9/18 , C12P17/04 , C12P41/001 , C12P41/005
摘要： A novel enzyme which is useful in the optical resolution of D,L-pantolactone via D-selective asymmetric hydrolysis and a gene encoding the the same are provided. The invention discloses the gene coding for a natural D-pantolactone hydrolase (for example, one originating in Fusarium oxysporum) or proteins having an activity substantially equivalent thereto; host cells transformed with DNA containing a nucleotide sequence coding for said protein, processes for producing said protein via using said host cells and uses of said proteins and host cells.
摘要翻译： 提供了可用于通过D-选择性不对称水解的D，L-泛酸内酯的光学拆分和编码其的基因的新型酶。 本发明公开了编码天然D-泛酸内酯水解酶（例如源于尖孢镰刀菌的一种）的基因或具有与其基本相当的活性的蛋白质; 用含有编码所述蛋白质的核苷酸序列的DNA转化的宿主细胞，通过使用所述宿主细胞产生所述蛋白质的过程和所述蛋白质和宿主细胞的用途。
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公开(公告)号：US5372940A
公开(公告)日：1994-12-13
申请号：US859439
申请日：1992-06-02
申请人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu
发明人： Keiji Sakamoto , Hideaki Yamada , Sakayu Shimizu
IPC分类号： C12N9/18 , C12P7/42 , C12P17/04 , C12P41/00 , C12R1/645 , C12R1/665 , C12R1/77 , C12R1/82 , C12R1/845 , C12P17/02 , C12N9/14
CPC分类号： C12P7/42 , C12N9/18 , C12P17/04 , C12P41/005
摘要： The invention is drawn to a new enzyme that selectively hydrolyzes D-pantolactone in D,L-pantolactone and has the following characteristics:(a) action: acts on pantolactone to produce the corresponding acid;(b) specificity for substrate: acts specifically on D-pantolactone but not on L-pantolactone;(c) pH stability: stable at pH5-9;(d) optimal pH: 7.0-7.5;(e) optimal temperature: ca. 50.degree. C.; and(f) effect of various metal ions or inhibitors: inhibited by Cd.sup.2+, Hg.sup.2+ or Cu.sup.2+ EDTA, as well as a process for the preparation thereof. The enzyme is preferably produced by cultivating the microorganisms of the genera Fusarium, Cylindrocarpon, and Gibberella, and most preferably the microorganism Fusarium oxysporum IFO 5942.
摘要翻译： PCT No.PCT / JP91 / 01351 Sec。 371日期：1992年6月2日 102（e）日期1992年6月2日PCT提交1991年10月4日PCT公布。 公开号WO92 / 06182 日期：1992年4月16日。本发明涉及一种在D，L-泛酸内酯中选择性水解D-泛酸内酯的新酶，具有以下特征：（a）作用：作用于泛酸内酯以产生相应的酸; （b）底物的特异性：特异性地对D-泛酸内酯作用，但不对L-泛酸内酯作用; （c）pH稳定性：pH5-9时稳定; （d）最佳pH：7.0-7.5; （e）最佳温度：约 50℃。 和（f）各种金属离子或抑制剂的作用：被Cd2 +，Hg2 +或Cu2 + EDTA抑制，以及其制备方法。 该酶优选通过栽培镰刀菌属，圆锥菌属和赤霉属的微生物，最优选微生物尖孢镰刀菌IFO 5942来产生。
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公开(公告)号：US5211953A
公开(公告)日：1993-05-18
申请号：US902854
申请日：1992-06-23
申请人： Yoshifumi Shinmen , Kengo Akimoto , Sumio Asami , Yoshihide Suwa , Yoshinori Kitagawa , Michihiro Sugano , Hideaki Yamada , Sakayu Shimizu
发明人： Yoshifumi Shinmen , Kengo Akimoto , Sumio Asami , Yoshihide Suwa , Yoshinori Kitagawa , Michihiro Sugano , Hideaki Yamada , Sakayu Shimizu
IPC分类号： A23D7/005 , A23L1/24 , A23L1/30 , A61K31/335 , A61K31/34
CPC分类号： A23D7/0056 , A23L27/60 , A23L33/10 , A61K31/335 , A61K31/34 , A23V2002/00 , Y10S514/811 , Y10S514/893 , Y10S514/894
摘要： A liver function improver, food, or drink comprising, as an effective ingredient, a dioxabicyclo[3.3.0]octane derivative, such as sesamin, sesaminol espisesamin, episesaminol, sesamolin, 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane, 2,6-bis-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane or 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenoxy)-3,7-dioxabicyclo[3.3.0]octane.
摘要翻译： 作为有效成分的二氧杂二环[3.3.0]辛烷衍生物，如芝麻素，芝麻素西塞米松，异芝麻酚，芝麻素，2-（3,4-亚甲二氧基苯基）-6-（ 3-甲氧基-4-羟基苯基）-3,7-二氧杂环环[3.3.0]辛烷，2,6-双 - （3-甲氧基-4-羟基苯基）-3,7-二氧杂双环[3.3.0]辛烷或 2-（3,4-亚甲二氧基苯基）-6-（3-甲氧基-4-羟基苯氧基）-3,7-二氧杂环己烯并[3.3.0]辛烷。
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公开(公告)号：US4229538A
公开(公告)日：1980-10-21
申请号：US28604
申请日：1979-04-09
申请人： Hideaki Yamada , Sakayu Shimizu , Yoshiki Tani
发明人： Hideaki Yamada , Sakayu Shimizu , Yoshiki Tani
CPC分类号： C12N9/93 , C12Q1/25 , Y10S435/875 , Y10S435/911
摘要： Acyl-CoA synthetase, having a high activity to C.sub.16 -C.sub.18 long chain fatty acids, is obtained by culturing strains belonging to various genera. As is known, acyl-CoA synthetase, having a strong activity to C.sub.16 -C.sub.18 long chain fatty acids, is generally obtained from liver of rat; however, it has now been discovered that acyl-CoA synthetase can be obtained from microorganisms and this enzyme is called acyl-CoA synthetase LCF-18. By use of acyl-CoA synthetase LCF-18 of the present invention which has a high activity to C.sub.16 -C.sub.18 long chain fatty acids, serum non-esterified fatty acid of human beings can be accurately determined, and it is very useful for diagnosis of diabetes and so forth.
摘要翻译： 通过培养属于各种属的菌株获得对C16-C18长链脂肪酸具有高活性的酰基辅酶A合成酶。 众所周知，对C16-C18长链脂肪酸具有强烈活性的酰基辅酶A合成酶通常由大鼠肝脏获得; 然而，现在已经发现可以从微生物获得酰基辅酶A合成酶，该酶称为酰基辅酶A合成酶LCF-18。 通过使用对C16-C18长链脂肪酸具有高活性的本发明的酰基-CoA合成酶LCF-18，可以准确测定人的血清非酯化脂肪酸，并且其对于诊断 糖尿病等。
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7.发明授权Process for production of dihomo-&ggr;-linolenic acid and lipid containing same 失效制备含有相似物质的二氧化γ-亚麻酸和脂质的方法公开(公告)号：US06602690B2
公开(公告)日：2003-08-05
申请号：US09761828
申请日：2001-01-18
IPC分类号： C12F764
CPC分类号： C12P7/6427 , C12P7/6472
摘要： A process for the production of dihomo-&ggr;-linolenic acid comprising the steps of culturing a microorganism having an ability to produce araquidonic acid and having a reduced or lost &Dgr;5 desaturase activity to produce dihomo-&ggr;-linolenic acid or a lipid containing dihomo-&ggr;-linolenic acid, and recovering the dihomo-&ggr;-linolenic acid.
摘要翻译： 一种生产二硫-γ-亚麻酸的方法，包括以下步骤：培养具有产生araquidonic酸的能力的微生物，并且具有减少或失去的DELTA5去饱和酶活性以产生二高-γ-亚麻酸或含有二氢-γ的脂质 亚麻酸，并回收二 - γ-亚麻酸。
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8.发明授权Process for production of highly unsaturated fatty acid having odd number of carbon atoms 失效生产具有碳原子数的高度不饱和脂肪酸的方法
公开(公告)号：US5128250A
公开(公告)日：1992-07-07
申请号：US321095
申请日：1989-03-09
CPC分类号： C12P7/6427 , C12P7/6472 , Y10S435/911
摘要： A process for the production of a highly unsaturated fatty acid having an odd number of carbon atoms by culturing a microorganism belong to the genus Mortierella and capable of producing the fatty acid; and a process for the production a highly unsaturated fatty acid having an odd number of carbon atoms, typically represented by 8,11,14-nonadecatrienoic acid, by culturing a microorganism capable of producing arachidonic acid.
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公开(公告)号：US4609626A
公开(公告)日：1986-09-02
申请号：US634208
申请日：1984-07-25
申请人： Hideaki Yamada , Sakayu Shimizu , Shozo Shiozaki
发明人： Hideaki Yamada , Sakayu Shimizu , Shozo Shiozaki
IPC分类号： C12N9/14 , C12R1/01 , C12R1/05 , C12R1/06 , C12R1/065 , C12R1/13 , C12R1/15 , C12R1/20 , C12R1/265 , C12R1/29 , C12R1/365 , C12R1/38 , C12R1/465 , C12R1/64 , C12R1/645 , C12R1/32
CPC分类号： C12N9/14 , Y10S435/822 , Y10S435/829 , Y10S435/83 , Y10S435/831 , Y10S435/843 , Y10S435/85 , Y10S435/863 , Y10S435/867 , Y10S435/872 , Y10S435/874 , Y10S435/886
摘要： A method for producing S-adenosyl-L-homocysteine hydrolase, which comprises cultivating a microorganism having the ability to produce S-adenosyl-L-homocysteine hydrolase within its cells in a nutrient medium to accumulate said hydrolase in the cells, said microorganism being a bacterium belonging to the genera Alcaligenes, Pseudomonas, Acinetobacter, Arthrobacter, Enterobacter, Rhodopseudomonas, Agrobacterium, Micrococcus, Corynebacterium, Brevibacterium, Chromobacterium, Xanthomonas, Flavobacterium, Cellulomonas, Azotobacter and Protaminobacter, or an actinomycete belonging to the genera Streptomyces, Mycobacterium, Nocardia, Streptoverticillium, Micromonospora, Micropolyspora, Streptosporangium and Microellobosporia; and then recovering S-adenosyl-L-homocysteine hydrolase from the cells.
摘要翻译： 一种S-腺苷-L-高半胱氨酸水解酶的制造方法，其特征在于，在营养培养基中培养具有在其细胞内产生S-腺苷-L-高半胱氨酸水解酶的能力的微生物，以在细胞中积聚所述水解酶，所述微生物为 属于产碱杆菌属，假单胞菌属，不动杆菌属，节杆菌属，肠杆菌属，红假单胞菌属，土壤杆菌属，微球菌属，棒状杆菌属，短杆菌属，色杆菌属，黄单胞菌属，黄杆菌属，单核细菌属，固氮菌属和Protaminobacter属的细菌或属于链霉菌属，分枝杆菌属，诺卡氏菌属， 链霉菌，小单孢菌，微孢子虫，链球菌属和微球菌属; 然后从细胞中回收S-腺苷-L-高半胱氨酸水解酶。
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公开(公告)号：US06406898B1
公开(公告)日：2002-06-18
申请号：US08836047
申请日：1997-09-19
IPC分类号： C12N914
CPC分类号： C12N9/18 , C12P17/04 , C12P41/001 , C12P41/005
摘要： A novel enzyme which is useful in the optical resolution of D,L-pantolactone via D-selective asymmetric hydrolysis and a gene encoding the the same are provided. The invention discloses the gene coding for a natural D-pantolactone hydrolase (for example, one originating in Fusarium oxysporum) or proteins having an activity substantially equivalent thereto; host cells transformed with DNA containing a nucleotide sequence coding for said protein, processes for producing said protein via using said host cells and uses of said proteins and host cells.
摘要翻译： 提供了可用于通过D-选择性不对称水解的D，L-泛酸内酯的光学拆分和编码其的基因的新型酶。 本发明公开了编码天然D-泛酸内酯水解酶（例如源于尖孢镰刀菌的一种）的基因或具有与其基本相当的活性的蛋白质; 用含有编码所述蛋白质的核苷酸序列的DNA转化的宿主细胞，通过使用所述宿主细胞产生所述蛋白质的过程和所述蛋白质和宿主细胞的用途。
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