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    Cell separation chip and cell culturing method using the same
    4.
    发明申请
    Cell separation chip and cell culturing method using the same 审中-公开
    细胞分离芯片和细胞培养方法使用相同

    公开(公告)号：US20080003678A1

    公开(公告)日：2008-01-03

    申请号：US11819681

    申请日：2007-06-28

    申请人： Akihiro Hattori ,  Hideyuki Terazono ,  Kenji Yasuda

    发明人： Akihiro Hattori ,  Hideyuki Terazono ,  Kenji Yasuda

    IPC分类号： C12N5/06 ,  C12M1/00

    CPC分类号： B01L3/502761 ,  B01L3/502753 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  C12M45/00 ,  C12M47/04 ,  G01N15/1031 ,  G01N15/12 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1484 ,  G01N2015/1081 ,  G01N2015/149

    摘要： An inexpensive cell analysis and separation apparatus using a flow channel formed on one surface of a substrate and a chip replaceable for each sample, and a method for culturing the separated cells without contamination, are provided. A flow channel for allowing a cell-containing buffer solution to flow is provided. Cells are detected in the middle of the flow channel, and separated to a plurality of downstream flow channels based on whether each cell fulfills a predetermined condition. A culturing tank for collecting the condition-fulfilling cells is covered with a semipermeable membrane at a top surface so as to prevent contamination during cell separation. When the cell separation is finished, the flow channel communicated with the culturing tank accommodating the condition-fulfilling cells is closed, and the culturing tank is cut off from the apparatus and put into a culturing device containing a predetermined medium to culture the cells.

    摘要翻译： 提供了使用形成在基板的一个表面上的流动通道和可替代每个样品的芯片的便宜的电池分析和分离装置，以及用于培养分离的电池而不被污染的方法。 提供了允许含细胞的缓冲溶液流动的流动通道。 基于每个小区是否满足预定条件，在流信道的中间检测小区，并将其分离成多个下游流信道。 用于收集状态满足细胞的培养槽在顶表面被半透膜覆盖，以防止细胞分离过程中的污染。 当细胞分离完成时，与容纳条件满足细胞的培养箱连通的流动通道关闭，并将培养箱从装置中切下并放入含有预定培养基的培养装置中以培养细胞。

    LIQUID REFLUX HIGH-SPEED GENE AMPLIFICATION DEVICE
    5.
    发明申请
    LIQUID REFLUX HIGH-SPEED GENE AMPLIFICATION DEVICE 有权
    液体REFLUX高速基因放大器件

    公开(公告)号：US20120077262A1

    公开(公告)日：2012-03-29

    申请号：US13138784

    申请日：2010-03-31

    申请人： Hiroyuki Takei ,  Hideyuki Terazono ,  Kenji Yasuda

    发明人： Hiroyuki Takei ,  Hideyuki Terazono ,  Kenji Yasuda

    IPC分类号： C12M1/40

    CPC分类号： B01L7/52 ,  B01L3/50851 ,  B01L3/50855 ,  B01L2300/185 ,  B01L2400/0622 ,  B01L2400/0633 ,  B01L2400/0644 ,  C12Q1/686 ,  F28D7/0008 ,  F28D15/00 ,  F28D2021/0077 ,  F28F27/02 ,  F28F2280/02

    摘要： The present invention provides a liquid reflux reaction control device comprising: a reaction vessel having one or a plurality of wells configured to accommodate a sample; a heat exchange vessel provided in contact with the reaction vessel so as to conduct heat to the reaction vessel, and comprising an inlet and an outlet respectively for introducing and draining a liquid of a predetermined temperature; a plurality of liquid reservoir tanks provided with a temperature-controllable heat source for maintaining liquids of predetermined temperatures; a tubular flow channel that connects the inlet and the outlet of the heat exchange vessel with the liquid reservoir tanks; a pump disposed on the tubular flow channel, and configured to circulate the liquid between the heat exchange vessel and the liquid reservoir tank; and a switching valve disposed on the tubular flow channel, and configured to control the flow of the circulating liquid, which controls the temperature of the reaction vessel to keep a desired temperature by switching the flows of the liquids of the predetermined temperatures from the plurality of liquid reservoir tanks into the heat exchange vessel at a predetermined time interval, wherein the amount of the sample is less than or equal to several μL per well, and the total volume of the circulating liquid is more than or equal to several tens of mL per liquid reservoir tank.

    摘要翻译： 本发明提供了一种液体回流反应控制装置，包括：反应容器，其具有构造成容纳样品的一个或多个孔; 设置为与反应容器接触以便将热量传导到反应容器的热交换容器，并且包括分别用于引入和排出预定温度的液体的入口和出口; 设置有用于保持预定温度的液体的温度可控热源的多个储液箱; 连接热交换容器的入口和出口与液体储存罐的管状流动通道; 泵，其设置在所述管状流动通道上，并且构造成使所述液体在所述热交换容器和所述储液箱之间循环; 以及设置在所述管状流动通道上的切换阀，并且被配置为控制所述循环液体的流动，所述流动控制所述反应容器的温度以通过将来自所述多个所述预定温度的所述预定温度的液体的流动切换来保持期望温度 液体储存罐以预定的时间间隔进入热交换容器，其中样品的量小于或等于每孔几μL，循环液体的总体积大于或等于几十mL， 液体储罐。

    Liquid reflux high-speed gene amplification device
    6.
    发明授权
    Liquid reflux high-speed gene amplification device 有权
    液体回流高速基因扩增装置

    公开(公告)号：US08900854B2

    公开(公告)日：2014-12-02

    申请号：US13138784

    申请日：2010-03-31

    申请人： Hiroyuki Takei ,  Hideyuki Terazono ,  Kenji Yasuda

    发明人： Hiroyuki Takei ,  Hideyuki Terazono ,  Kenji Yasuda

    IPC分类号： C12M1/00 ,  C12M3/00 ,  C12M1/22 ,  B01L3/00 ,  B01L7/00 ,  F28F27/02 ,  F28D7/00 ,  F28D15/00 ,  F28D21/00

    CPC分类号： B01L7/52 ,  B01L3/50851 ,  B01L3/50855 ,  B01L2300/185 ,  B01L2400/0622 ,  B01L2400/0633 ,  B01L2400/0644 ,  C12Q1/686 ,  F28D7/0008 ,  F28D15/00 ,  F28D2021/0077 ,  F28F27/02 ,  F28F2280/02

    摘要： The present invention provides a liquid reflux reaction control device comprising: a reaction vessel having one or a plurality of wells configured to accommodate a sample; a heat exchange vessel provided in contact with the reaction vessel so as to conduct heat to the reaction vessel, and comprising an inlet and an outlet respectively for introducing and draining a liquid of a predetermined temperature; a plurality of liquid reservoir tanks provided with a temperature-controllable heat source for maintaining liquids of predetermined temperatures; a tubular flow channel that connects the inlet and the outlet of the heat exchange vessel with the liquid reservoir tanks; a pump disposed on the tubular flow channel, and configured to circulate the liquid between the heat exchange vessel and the liquid reservoir tank; and a switching valve disposed on the tubular flow channel, and configured to control the flow of the circulating liquid, which controls the temperature of the reaction vessel to keep a desired temperature by switching the flows of the liquids of the predetermined temperatures from the plurality of liquid reservoir tanks into the heat exchange vessel at a predetermined time interval, wherein the amount of the sample is less than or equal to several μL per well, and the total volume of the circulating liquid is more than or equal to several tens of mL per liquid reservoir tank.

    摘要翻译： 本发明提供了一种液体回流反应控制装置，包括：反应容器，其具有构造成容纳样品的一个或多个孔; 设置为与反应容器接触以便将热量传导到反应容器的热交换容器，并且包括分别用于引入和排出预定温度的液体的入口和出口; 设置有用于保持预定温度的液体的温度可控热源的多个储液箱; 连接热交换容器的入口和出口与液体储存罐的管状流动通道; 泵，其设置在所述管状流动通道上，并且构造成使所述液体在所述热交换容器和所述储液箱之间循环; 以及设置在所述管状流动通道上的切换阀，并且被配置为控制所述循环液体的流动，所述流动控制所述反应容器的温度以通过将来自所述多个所述预定温度的所述预定温度的液体的流动切换来保持期望温度 液体储存罐以预定的时间间隔进入热交换容器，其中样品的量小于或等于每孔几μL，循环液体的总体积大于或等于几十mL， 液体储罐。

    Device for concentrating and separating cells
    7.
    发明授权
    Device for concentrating and separating cells 有权
    用于浓缩和分离细胞的装置

    公开(公告)号：US09109197B2

    公开(公告)日：2015-08-18

    申请号：US13138785

    申请日：2010-03-31

    申请人： Kenji Yasuda ,  Masahito Hayashi ,  Akihiro Hattori

    发明人： Kenji Yasuda ,  Masahito Hayashi ,  Akihiro Hattori

    IPC分类号： C12M1/34 ,  C12M3/00 ,  C12M1/00

    CPC分类号： C12M47/04 ,  B01D21/283 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0439

    摘要： Provided is a device for concentrating and separating cells, which has a function for continuously concentrating cells; a function for then continuously arranging the concentrated cells in predetermined regions of a flow path; a function for simultaneously identifying shape and fluorescent emission in one-cell units on the basis of cell concentration and purification images, which serve to continuously separate and purify cells that have different properties in that they are either attracted to or repelled by an induction electrophoresis force of a predetermined frequency; and a function for identifying cells on the basis of this shape and fluorescent emission information and thereby separating and purifying the cells.

    摘要翻译： 提供了用于浓缩和分离细胞的装置，其具有用于连续浓缩细胞的功能; 然后连续地将浓缩的细胞排列在流路的预定区域中的功能; 基于细胞浓度和纯化图像同时鉴定单细胞单位形状和荧光发射的功能，其用于连续分离和纯化具有不同性质的细胞，因为它们被感应电泳力吸引或排斥 的预定频率; 以及基于该形状和荧光发射信息鉴别细胞​​，从而分离和纯化细胞的功能。

    DEVICE FOR CONCENTRATING AND SEPARATING CELLS
    8.
    发明申请
    DEVICE FOR CONCENTRATING AND SEPARATING CELLS 有权
    用于浓缩和分离细胞的装置

    公开(公告)号：US20120088295A1

    公开(公告)日：2012-04-12

    申请号：US13138785

    申请日：2010-03-31

    申请人： Kenji Yasuda ,  Masahito Hayashi ,  Akihiro Hattori

    发明人： Kenji Yasuda ,  Masahito Hayashi ,  Akihiro Hattori

    IPC分类号： C12M1/42 ,  B03C5/02 ,  C12M1/00 ,  B01D43/00

    CPC分类号： C12M47/04 ,  B01D21/283 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0439

    摘要： Provided is a device for concentrating and separating cells, which has a function for continuously concentrating cells; a function for then continuously arranging the concentrated cells in predetermined regions of a flow path; a function for simultaneously identifying shape and fluorescent emission in one-cell units on the basis of cell concentration and purification images, which serve to continuously separate and purify cells that have different properties in that they are either attracted to or repelled by an induction electrophoresis force of a predetermined frequency; and a function for identifying cells on the basis of this shape and fluorescent emission information and thereby separating and purifying the cells.

    摘要翻译： 提供了用于浓缩和分离细胞的装置，其具有用于连续浓缩细胞的功能; 然后连续地将浓缩的细胞排列在流路的预定区域中的功能; 基于细胞浓度和纯化图像同时鉴定单细胞单位形状和荧光发射的功能，其用于连续分离和纯化具有不同性质的细胞，因为它们被感应电泳力吸引或排斥 的预定频率; 以及基于该形状和荧光发射信息鉴别细胞​​，从而分离和纯化细胞的功能。

    MODEL CELL CHIP, APPARATUS FOR EVALUATING DRUG EFFECT USING THE MODEL CELL CHIP AND METHOD OF EVALUATING DRUG EFFECT
    9.
    发明申请
    MODEL CELL CHIP, APPARATUS FOR EVALUATING DRUG EFFECT USING THE MODEL CELL CHIP AND METHOD OF EVALUATING DRUG EFFECT 有权
    模型细胞芯片，使用模型细胞芯片评估药物效应的装置和评估药物效应的方法

    公开(公告)号：US20100178692A1

    公开(公告)日：2010-07-15

    申请号：US12663468

    申请日：2008-06-06

    申请人： Kenji Yasuda ,  Atsushi Sugiyama ,  Kentaro Ando ,  Fumimasa Nomura ,  Hideyuki Terazono ,  Tomoyuki Kaneko ,  Mamoru Fukushima

    发明人： Kenji Yasuda ,  Atsushi Sugiyama ,  Kentaro Ando ,  Fumimasa Nomura ,  Hideyuki Terazono ,  Tomoyuki Kaneko ,  Mamoru Fukushima

    IPC分类号： C12M1/34

    CPC分类号： G01N33/5061 ,  C12N2503/02 ,  G01N33/4836 ,  G01N33/54366

    摘要： The present invention provides an apparatus for evaluating a drug effect enabling on-chip evaluation of the effect of a drug while the drug is acting on hERG-expressing cells. The present invention also provides a myocardial toxicity test apparatus and method therefor enabling in vitro myocardial toxicity testing that has previously been performed in vivo.A pulsating cell population and hERG-expressing cells (target model cells) are suitably isolated and arranged on a transparent substrate so that the two form gap junctions. The hERG-expressing cells are arranged on transparent electrodes provided on the transparent substrate. The hERG-expressing cells are exposed to a flow of a liquid containing a drug such that the drug acts thereon. The difference between the normal pulsation of hERG-expressing cells and the pulsation when a drug is acting thereon is captured via electric signals obtained from electrodes, and the properties of the change in potential are evaluated.

    摘要翻译： 本发明提供了一种药物效应评价装置，其能够在药物作用于表达hERG的细胞的同时对药物的作用进行片上评价。 本发明还提供一种心肌毒性试验装置及其方法，其能够进行体外心肌毒性试验，其先前已在体内进行。 脉动细胞群体和表达hERG的细胞（靶模型细胞）被适当地分离并排列在透明基底上，使得两个形成间隙连接。 hERG表达细胞设置在设置在透明基板上的透明电极上。 将表达hERG的细胞暴露于含有药物的液体流中，使药物作用于其上。 通过从电极获得的电信号捕获hERG表达细胞的正常脉动与药物作用于其上的脉动之间的差异，并评估电位变化的性质。

    CARDIAC REENTRY MODEL CHIP AND APPARATUS AND METHOD FOR EVALUATING DRUG USING THE CARDIAC REENTRY MODEL CHIP
    10.
    发明申请
    CARDIAC REENTRY MODEL CHIP AND APPARATUS AND METHOD FOR EVALUATING DRUG USING THE CARDIAC REENTRY MODEL CHIP 审中-公开
    心脏复苏模型芯片和装置以及使用心脏重吸收模型评估药物的方法

    公开(公告)号：US20100173351A1

    公开(公告)日：2010-07-08

    申请号：US12663441

    申请日：2008-06-06

    申请人： Kenji Yasuda ,  Atsushi Sugiyama ,  Kentaro Ando ,  Fumimasa Nomura ,  Hideyuki Terazono ,  Tomoyuki Kaneko ,  Mamoru Fukushima

    发明人： Kenji Yasuda ,  Atsushi Sugiyama ,  Kentaro Ando ,  Fumimasa Nomura ,  Hideyuki Terazono ,  Tomoyuki Kaneko ,  Mamoru Fukushima

    IPC分类号： C12Q1/02 ,  C12M1/00

    CPC分类号： G01N33/5061 ,  G01N33/48728 ,  G01N33/5438

    摘要： A chip has been developed that can accurately measure cell potential and cell morphology on a single cell basis. The chip also constitutes a cardiac model that comprises a closed loop whereupon cardiomyocytes and fibroblasts are suitably dispersed and arranged, and that can evaluate the effects of a drug thereon. An in vitro cardiac reentry model chip is fabricated by constructing a closed loop comprising cardiomyocytes and fibroblasts arrayed on transparent electrodes formed on a transparent substrate by using a constitution where single cells are enclosed in a specific spatial configuration. A pulse wave of a random cardiomyocyte or a specific cardiomyocyte is propagated on both sides of the loop, and the pulsation status of the cells in the loop is detected electrically. A drug is applied to this cardiac reentry model chip, and the benefit or toxicity of the drug to cardiomyocytes is evaluated by measuring the cell potentials of individual cells.

    摘要翻译： 已经开发了可以在单个细胞基础上精确测量细胞电位和细胞形态的芯片。 该芯片还构成了心脏模型，其包括闭合环，由此心肌细胞和成纤维细胞适当地分散和排列，并且可以评价药物对其的影响。 通过使用单细胞被封闭在特定的空间结构中的构造，构建包括心肌细胞和成纤维细胞的闭环，制成排列在透明电极上形成的透明电极上的闭环回路模型芯片。 随机心肌细胞或特定心肌细胞的脉搏波在环的两侧传播，电路中检测到细胞的脉动状态。 将药物应用于该心脏再入模型芯片，并通过测量个体细胞的细胞电位来评估药物对心肌细胞的益处或毒性。