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    MODIFIED PROTEIN AND METHOD FOR USING SAME
    1.
    发明申请
    MODIFIED PROTEIN AND METHOD FOR USING SAME 审中-公开

    公开(公告)号：US20200299744A1

    公开(公告)日：2020-09-24

    申请号：US16765658

    申请日：2019-01-30

    Applicant: HITACHI, LTD.

    Inventor： Masashi MARUYAMA ,  Keisuke SHIBUYA

    IPC: C12P21/00 ,  C07K14/62 ,  C08F8/30 ,  C08F20/56

    Abstract: The present invention pertains to a means and method for, from a mixture (cell culture) of cells, a medium component, and a useful substance, recovering the first two using a single process. Specifically, the present invention pertains to a polypeptide modified with a stimulus-responsive polymer, characterized in that the stimulus-responsive polymer responds to a stimulus to induce a change in the physical properties of the modified polypeptide overall, and the change is reversible or pseudo-reversible.

    CELL CULTURE MEDIUM, AND CELL CULTURE APPARATUS AND CELL CULTURE METHOD EACH USING SAME
    3.
    发明申请
    CELL CULTURE MEDIUM, AND CELL CULTURE APPARATUS AND CELL CULTURE METHOD EACH USING SAME 审中-公开

    公开(公告)号：US20200063106A1

    公开(公告)日：2020-02-27

    申请号：US16462954

    申请日：2017-10-31

    Applicant: HITACHI, LTD.

    Inventor： Keisuke SHIBUYA ,  Masashi MARUYAMA ,  Takeyuki KONDOU

    IPC: C12N5/071 ,  C12M1/06 ,  C12M1/00 ,  C12M1/34

    Abstract: A cell culture apparatus (1) of the present invention comprises: a culture vessel (2) in which a cell culture medium comprising at least one of culture components composed of a conjugate with a stimuli-responsive polymer is stored and cells are cultured in the medium; a stimulus-applying mechanism (4) that applies a predetermined stimulus to the conjugate so as to induce a predetermined change of the stimuli-responsive polymer in response to the stimulus; and, a separation mechanism (5) that separates at least a part of the medium components except for the conjugate from the cell culture medium, while leaving the conjugate in the cell culture medium, on the basis of a property change of the stimuli-responsive polymer.

    ADSORBENT
    4.
    发明申请
    ADSORBENT 审中-公开
    吸引力

    公开(公告)号：US20160017059A1

    公开(公告)日：2016-01-21

    申请号：US14798608

    申请日：2015-07-14

    Applicant: HITACHI, LTD.

    Inventor： Masashi MARUYAMA ,  Yasuhiko TADA ,  Keisuke SHIBUYA

    IPC: C07K17/06 ,  C07K1/22

    CPC classification number: C07K17/06 ,  B01D15/3809 ,  B01J20/289 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3225 ,  B01J20/3274 ,  B01J20/3278 ,  B01J20/3285 ,  B01J2220/58 ,  C07K1/22

    Abstract: An adsorbent of the present invention includes a support; and a protein having an adsorption site for adsorbing a target substance, in which the protein includes a reversible binding site and a covalent binding site, the support includes an orientation-controlling site forming a reversible bond with the reversible binding site, and an immobilization site forming a covalent bond with the covalent binding site, the covalent binding site includes a nucleophilic functional group, and the immobilization site includes a functional group capable of reacting with the nucleophilic functional group by a nucleophilic substitution or a nucleophilic addition. Thus, it is possible to provide an adsorbent in which the utilization efficiency of the adsorption site of the protein is improved. As a result, the protein amount can be reduced, and the step for storing a culture solution can be simplified due to the faster purification step.

    Abstract translation: 本发明的吸附剂包括载体; 以及具有吸附目标物质的吸附部位的蛋白质，其中所述蛋白质包含可逆结合位点和共价结合位点，所述载体包括与可逆结合位点形成可逆键的取向控制位点和固定位点 与共价结合位点形成共价键，共价结合位点包括亲核官能团，并且固定位点包括能够通过亲核取代或亲核加成与亲核官能团反应的官能团。 因此，可以提供蛋白质的吸附部位的利用效率提高的吸附剂。 结果，可以降低蛋白质量，并且由于更快的纯化步骤，可以简化用于储存培养溶液的步骤。

    Preanalysis Treatment Method for Sample, and Sample Pretreatment System
    5.
    发明申请
    Preanalysis Treatment Method for Sample, and Sample Pretreatment System 有权

    公开(公告)号：US20220317130A1

    公开(公告)日：2022-10-06

    申请号：US17713621

    申请日：2022-04-05

    Applicant: Hitachi, Ltd.

    Inventor： Masashi MARUYAMA ,  Hirotaka SAKUMA ,  Eric OFOSU-TWUM

    IPC: G01N33/68 ,  G01N1/38

    Abstract: Objects of the disclosure are to provide a preanalysis treatment method for a sample, which can reduce mixing of a reagent into the sample, and to provide a sample pretreatment system capable of performing the preanalysis treatment method. An aspect of the present embodiment is a preanalysis treatment method for a sample, which includes a step A of mixing the sample and a reagent A containing a support and a reducing agent immobilized on the support, a step B of mixing the sample and a reagent B containing a support and an enzyme immobilized on the support, a step X-A of, after the step A, separating the reagent A and a supernatant from each other, and a step X-B of, after the step B, separating the reagent B and a supernatant from each other.

    AUTOMATIC CULTURE SYSTEM, AND CELL CULTURE METHOD
    7.
    发明申请
    AUTOMATIC CULTURE SYSTEM, AND CELL CULTURE METHOD 审中-公开

    公开(公告)号：US20200318059A1

    公开(公告)日：2020-10-08

    申请号：US16754430

    申请日：2018-10-18

    Applicant: HITACHI, LTD.

    Inventor： Masashi MARUYAMA ,  Masaki MATSUMORI

    IPC: C12M1/36 ,  C12M1/34 ,  C12N9/50

    Abstract: The purpose of the present invention is to provide an automatic culture system whereby cells can be released from a vessel by a simple constitution and damage on the cells can be minimized In the automatic culture system according to the present invention, cells are cultured in a stimulus-responsive culture vessel and then the cells are released from the culture vessel by stimulating the culture vessel with a solution which is supplied from another vessel (see FIG. 3).

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