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    Novel immunocytostaining methods for enhanced dye ratio discrimination in rare event detection
    1.
    发明申请
    Novel immunocytostaining methods for enhanced dye ratio discrimination in rare event detection 有权
    用于在罕见事件检测中增强染料比率鉴别的新型免疫细胞染色方法

    公开(公告)号：US20070166770A1

    公开(公告)日：2007-07-19

    申请号：US11333731

    申请日：2006-01-17

    申请人： Huangpin Hsieh ,  Nicole Lazarus ,  Robert Krivacic ,  Douglas Curry ,  Richard Bruce

    发明人： Huangpin Hsieh ,  Nicole Lazarus ,  Robert Krivacic ,  Douglas Curry ,  Richard Bruce

    IPC分类号： G01N33/567

    CPC分类号： G01N1/30 ,  G01N15/1468 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/56966 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2021/6484 ,  G01N2201/0833

    摘要： A method is provided for preparing a sample containing potential cells of interest and of using a laser of a laser based system for novel excitation and emission collection, and data usage including use of obtained data for direct and ratio based measurements. The prepared sample is configured to emit signals having spectral characteristics sufficient to permit filtering to differentiate and eliminate most false positives from true positives among acquired imaging events, in an imaging system employing a laser spot having a range of diameters from 1 to 20 μm or greater and which excites the fluorescence in a conventional or novel manner. These filtered events may be subsequently imaged and confirmed with another higher resolution device such as a fluorescent microscope in a short amount of time.

    摘要翻译： 提供了一种制备包含潜在感兴趣的细胞的样品并使用基于激光的系统的激光进行新颖的激发和发射采集以及数据使用的方法，包括使用获得的数据进行直接和基于比率的测量。 所制备的样品被配置为发射具有足够的光谱特性的信号，以在使用具有1至20μm或更大直径范围的激光光斑的成像系统中进行滤光以区分并消除所获得的成像事件之间的真阳性的大多数假阳性 并以常规或新颖的方式激发荧光。 这些过滤的事件可以随后在较短的时间内用另一种较高分辨率的装置例如荧光显微镜进行成像和证实。

    Sample manipulator
    2.
    发明申请
    Sample manipulator 有权
    样品机械手

    公开(公告)号：US20060038120A1

    公开(公告)日：2006-02-23

    申请号：US10921556

    申请日：2004-08-19

    申请人： Meng Lean ,  Francisco Torres ,  Huangpin Hsieh ,  Armin Volkel ,  Bryan Preas ,  Scott Elrod ,  John Fitch ,  Richard Bruce

    发明人： Meng Lean ,  Francisco Torres ,  Huangpin Hsieh ,  Armin Volkel ,  Bryan Preas ,  Scott Elrod ,  John Fitch ,  Richard Bruce

    IPC分类号： H01J49/00

    CPC分类号： B03C1/253

    摘要： A sample manipulator that utilizes electrostatic traveling waves to selectively displace one or more samples deposited on its face is disclosed. The sample manipulator enables an operator to perform a wide variety of processes upon the deposited samples. Also disclosed are strategies for separating two or more samples, focusing a sample, and passing a reagent through a sample, all conducted on the face of the sample manipulator.

    摘要翻译： 公开了一种使用静电行波选择性地移动沉积在其表面上的一个或多个样品的样品操纵器。 样品操纵器使得操作者能够在沉积的样品上执行各种各样的处理。 还公开了用于分离两个或更多个样品，聚焦样品并使试剂通过样品的策略，全部在样品操纵器的表面上进行。

    Led or laser enabled real-time PCR system and spectrophotometer
    3.
    发明申请
    Led or laser enabled real-time PCR system and spectrophotometer 有权
    LED或激光启用实时PCR系统和分光光度计

    公开(公告)号：US20050133724A1

    公开(公告)日：2005-06-23

    申请号：US10739706

    申请日：2003-12-18

    申请人： Huangpin Hsieh ,  Michael Kneissl

    发明人： Huangpin Hsieh ,  Michael Kneissl

    IPC分类号： B01L7/00 ,  G01J5/02 ,  G01N21/03 ,  G01N21/25 ,  G01N21/27 ,  G01N21/31 ,  G01N21/64

    CPC分类号： G01N21/6454 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0829 ,  G01N21/0303 ,  G01N21/253 ,  G01N21/272 ,  G01N21/31 ,  G01N21/33 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2201/0627

    摘要： A system for conducting a polymerase chain reaction (PCR) assay upon a collection of samples is disclosed. The PCR assay is performed by absorption detection. The system includes a multi-well plate which is adapted to retain a collection of sample wells. This system includes a thermal cycler for the multi-well plate. The system additionally includes a collection of photodetectors, and a corresponding number of light sources. The light sources are positioned such that light emitted from each of the respective light sources passes through a corresponding well retained in the multi-well plate and to a corresponding photodetector. The system also includes a processor or other means for analyzing the output signals from the photodetectors. In certain versions of the system, ultra-violet light is used.

    摘要翻译： 公开了在收集样品时进行聚合酶链反应（PCR）测定的系统。 通过吸收检测进行PCR测定。 该系统包括多孔板，其适于保留一组样品孔。 该系统包括用于多孔板的热循环仪。 该系统还包括光电检测器的集合和相应数量的光源。 光源被定位成使得从每个相应光源发射的光穿过保持在多孔板中的对应的阱和相应的光电检测器。 该系统还包括用于分析来自光电检测器的输出信号的处理器或其它装置。 在系统的某些版本中，使用紫外光。

    Rare cell detection using flat-panel imager and chemiluminescent or radioisotopic tags
    8.
    发明申请
    Rare cell detection using flat-panel imager and chemiluminescent or radioisotopic tags 有权
    使用平板成像仪和化学发光或放射性同位素标签进行稀有细胞检测

    公开(公告)号：US20060051816A1

    公开(公告)日：2006-03-09

    申请号：US10937009

    申请日：2004-09-09

    申请人： Huangpin Hsieh ,  JengPeng Lu

    发明人： Huangpin Hsieh ,  JengPeng Lu

    IPC分类号： G01N33/542 ,  G06K9/00 ,  C12M1/34

    CPC分类号： G01N33/58 ,  G01N33/56966

    摘要： Disclosed is a system using a large area flat panel imager which is specifically adapted for rare cell detection methods. The system generally includes an imager having a sample receiving surface which can provide a digital or electronic image of a sample deposited on the surface. The system also includes a selectively positionable microscope and/or camera which are used to obtain high resolution images of the deposited samples. An electronic controller can also be used in conjunction with the imager, microscope, and/or camera to selectively position at least one of those components to focus on desired regions of the deposited sample. The noted system is particularly adapted for use with chemiluminescence or other tagging technologies.

    摘要翻译： 公开了一种特别适用于稀有细胞检测方法的大面积平板成像仪的系统。 该系统通常包括具有样品接收表面的成像器，其可以提供沉积在表面上的样品的数字或电子图像。 该系统还包括用于获得沉积样品的高分辨率图像的可选择性定位的显微镜和/或照相机。 电子控制器还可以与成像器，显微镜和/或照相机一起使用，以选择性地将这些部件中的至少一个放置在聚焦在沉积样品的期望区域上。 所述系统特别适用于化学发光或其他标签技术。

    Process for preparing albumin protein conjugated oligonucleotide probes
    9.
    发明申请
    Process for preparing albumin protein conjugated oligonucleotide probes 失效

    公开(公告)号：US20050244884A1

    公开(公告)日：2005-11-03

    申请号：US11173635

    申请日：2005-07-01

    申请人： Huangpin Hsieh

    发明人： Huangpin Hsieh

    IPC分类号： A61K47/48 ,  C12Q1/68

    CPC分类号： A61K47/643 ,  Y10T436/143333

    摘要： A technique for forming conjugates of proteins and oligonucleotides is disclosed in which conjugation is performed while the oligonucleotide is attached to support media such as glass beads. The conjugated product may then be readily removed from the support media. Also disclosed are the conjugated products formed by this technique. The present invention is particularly directed to conjugates of albumin proteins and specifically to bovine serum albumin (BSA).

    Concentration and focusing of bio-agents and micron-sized particles using traveling wave grids
    10.
    发明申请
    Concentration and focusing of bio-agents and micron-sized particles using traveling wave grids 有权
    使用行波网格集中和聚焦生物剂和微米级颗粒

    公开(公告)号：US20050123992A1

    公开(公告)日：2005-06-09

    申请号：US10727289

    申请日：2003-12-03

    申请人： Armin Volkel ,  Meng Lean ,  Huangpin Hsieh ,  Jurgen Daniel

    发明人： Armin Volkel ,  Meng Lean ,  Huangpin Hsieh ,  Jurgen Daniel

    IPC分类号： C12M1/34 ,  G01N33/53 ,  G01N33/543

    CPC分类号： G01N33/5438

    摘要： Several traveling wave grid systems are disclosed that may be used to concentrate and form highly localized regions of bio-agents or other charged species. In addition, specific detection systems are described that enable currently available detectors and sensors, including those to be developed in the future, to be used for measuring the presence and concentration of certain bio-agents or charged particles, which otherwise are present at concentrations too low to readily detect or measure.

    摘要翻译： 公开了几种可以用于浓缩和形成高度局部化的生物剂或其他带电物质区域的行波系统。 此外，描述了使得目前可用的检测器和传感器（包括将来要开发的检测器和传感器）能够用于测量某些生物试剂或带电粒子的存在和浓度的具体检测系统，否则其浓度也是以浓度存在 低易于检测或测量。