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    Device and method for printing biomolecules onto substrate using electrohydrodynamic effect
    6.
    发明申请
    Device and method for printing biomolecules onto substrate using electrohydrodynamic effect 审中-公开

    公开(公告)号：US20050214799A1

    公开(公告)日：2005-09-29

    申请号：US10982072

    申请日：2004-11-04

    申请人： Hye-jung Cho ,  Jeong-gun Lee

    发明人： Hye-jung Cho ,  Jeong-gun Lee

    IPC分类号： G01N33/53 ,  B01J19/00 ,  B01L3/02 ,  C12N15/09 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/68 ,  G01N35/10 ,  G01N37/00 ,  C12Q1/68 ,  B41J2/01 ,  C12M1/34

    CPC分类号： B01L3/0268 ,  B01J2219/00371 ,  B01J2219/00527 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01L3/0262 ,  B01L2300/0838 ,  B01L2400/027 ,  B01L2400/0415 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

    摘要： A device and a method for printing biomolecules onto a substrate using an electrohydrodynamic (EHD) effect are provided. The device for printing biomolecules onto a substrate using an electrohydrodynamic (EHD) effect includes at least one capillary having an outlet through which a feeding solution of biomolecules selected from the group consisting of nucleic acids, proteins, and oligopeptides is discharged, the nucleic acids being selected from the group consisting of probe DNA, RNA, peptide nucleic acid (PNA), and LNA and the proteins being selected from the group consisting of antigen and antibody; a printer body supporting the at least one capillary; a substrate below the outlet having a target surface onto which the biomolecules are deposited; a first electric field forming electrode located on the printer body around the circumference of the outlet; a second electric field forming electrode spaced apart from the first electrode by a predetermined distance; and a voltage applying unit which is electrically connected to the first electrode and the second electrode to apply an alternating current (AC) voltage between the first electrode and the second electrode so that an electric field may be formed around the biomolecule solution suspended in the outlet, and due to the interaction of the electric field and a difference in dielectric constant between the biomolecule solution having a free surface and the surrounding atmosphere, the electric force acts inward on the biomolecule solution from the surroundings, thereby dropping a predetermined amount of the biomolecule solution onto the target surface of the substrate. By using an EHD effect, the advantages of the device and method are that the process is quick, the biomolecule spots are uniform and easily aligned, and especially it is possible to spot the biomolecules correctly and reproducibly even when the printer body is not fully aligned with the substrate. In addition, the device and method are applicable to proteins which are sensitive to heat and biomolecules which have neutral charges.

    Device for printing biomolecules on substrate using electrohydrodynamic effect
    10.
    发明申请
    Device for printing biomolecules on substrate using electrohydrodynamic effect 审中-公开
    使用电动力学效应在基板上印刷生物分子的装置

    公开(公告)号：US20060255267A1

    公开(公告)日：2006-11-16

    申请号：US11418602

    申请日：2006-05-05

    申请人： Jeong-gun Lee ,  Jeong-woo Choi ,  Nam Huh ,  Hye-jung Cho

    发明人： Jeong-gun Lee ,  Jeong-woo Choi ,  Nam Huh ,  Hye-jung Cho

    IPC分类号： G21K7/00

    CPC分类号： B01L3/0244 ,  B01J2219/0036 ,  B01J2219/00378 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01L3/0241 ,  B01L2300/0819

    摘要： Provided is a device for printing a biomolecule solution onto a substrate using an electrohydrodynamic (EHD) effect. The device can deposit drops of the biomolecule solution with small size and volume and print more of the biomolecule solution onto a substrate, thereby enabling the manufacture of a high density biochip. The device includes a first electric field forming electrode that is shaped like a needle, is made of a conductive material, is disposed vertically, and includes an accommodating area in which the biomolecule solution is accommodated and an outlet formed on a bottom end of the accommodating area through which the biomolecule solution is discharged; a substrate disposed below the first electric field forming electrode, and including a target surface onto which the biomolecule solution discharged from the outlet of the first electric field forming electrode is deposited; a second electric field forming electrode made of a conductive material and disposed below the first electric field forming electrode; and a voltage applying device which is electrically connected to the first and second electric field forming electrodes to apply a voltage between the first and second electric field forming electrodes so that an electric field is generated around the biomolecule solution suspended in the outlet, wherein, due to the interaction between the electric field and a difference between dielectric constants of the biomolecule solution having a free surface and the surrounding atmosphere, the electric force acts inward on the biomolecule solution, thereby causing a predetermined amount of the biomolecule solution to drop onto the target surface of the substrate.

    摘要翻译： 提供了使用电动力学（EHD）效应将生物分子溶液印刷到基底上的装置。 该装置可以沉积小尺寸和体积的生物分子溶液，并将更多的生物分子溶液印刷到基底上，从而能够制造高密度生物芯片。 该装置包括成形为针状的第一电场形成电极，由导电材料制成，垂直设置，并且包括其中容纳有生物分子溶液的容纳区域和形成在容纳物的底端的出口 生物分子溶液排出的区域; 设置在所述第一电场形成电极下方的基板，并且包括从所述第一电场形成用电极的出口排出的生物分子溶液沉积在其上的目标表面; 由导电材料制成并设置在第一电场形成电极下方的第二电场形成电极; 以及电压施加装置，其电连接到第一和第二电场形成电极，以在第一和第二电场形成电极之间施加电压，使得在悬浮在出口中的生物分子溶液周围产生电场，其中，由于 对于电场与具有自由表面和周围大气的生物分子溶液的介电常数之间的差异的相互作用，电力向内作用于生物分子溶液，从而使预定量的生物分子溶液落到目标上 基板的表面。