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    Electric charge concentration type droplet dispensing device having nonconductive capillary nozzle
    1.
    发明授权
    Electric charge concentration type droplet dispensing device having nonconductive capillary nozzle 有权
    具有非导电毛细管喷嘴的电荷浓度型液滴分配装置

    公开(公告)号：US07677482B2

    公开(公告)日：2010-03-16

    申请号：US11781714

    申请日：2007-07-23

    申请人： Beom-seok Lee ,  Jeong-gun Lee ,  Hye-jung Cho

    发明人： Beom-seok Lee ,  Jeong-gun Lee ,  Hye-jung Cho

    IPC分类号： B05B5/00

    CPC分类号： B05B5/0255 ,  B01J2219/00367 ,  B01J2219/00369 ,  B01J2219/00371 ,  B01L3/0268 ,  B01L2300/0838 ,  B01L2300/161 ,  B01L2400/027 ,  B01L2400/0406 ,  B01L2400/0487 ,  B05B1/14 ,  B05B5/0533 ,  B41J2/06

    摘要： Provided is a droplet dispensing device having a nonconductive capillary nozzle. The droplet dispensing device comprises: a nonconductive capillary nozzle disposed in a downward position; a pump connected with the nonconductive capillary nozzle through a hermetically sealed fluid tube and generating a negative pressure to decrease the influence of gravity on a solution within the nonconductive capillary nozzle and the fluid tube; and an open circuit voltage supplier applying a voltage to the solution. The droplet dispensing device supplies the solution by capillary force to regularly maintain the shape of a droplet surface in the tip of the nonconductive capillary nozzle without using a separate driving device.

    摘要翻译： 提供了具有非导电毛细管喷嘴的液滴分配装置。 液滴分配装置包括：设置在向下位置的非导电毛细管喷嘴; 泵，其通过气密密封的流体管与非导电毛细管喷嘴连接并产生负压，以减少重力对非导电毛细管喷嘴和流体管内的溶液的影响; 以及向该溶液施加电压的开路电压供给器。 液滴分配装置通过毛细管力提供溶液以在不使用单独的驱动装置的情况下规定地保持非导电毛细管喷嘴的尖端中的液滴表面的形状。

    Device and method for printing biomolecules onto substrate using electrohydrodynamic effect
    10.
    发明申请
    Device and method for printing biomolecules onto substrate using electrohydrodynamic effect 审中-公开

    公开(公告)号：US20050214799A1

    公开(公告)日：2005-09-29

    申请号：US10982072

    申请日：2004-11-04

    申请人： Hye-jung Cho ,  Jeong-gun Lee

    发明人： Hye-jung Cho ,  Jeong-gun Lee

    IPC分类号： G01N33/53 ,  B01J19/00 ,  B01L3/02 ,  C12N15/09 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/68 ,  G01N35/10 ,  G01N37/00 ,  C12Q1/68 ,  B41J2/01 ,  C12M1/34

    CPC分类号： B01L3/0268 ,  B01J2219/00371 ,  B01J2219/00527 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01L3/0262 ,  B01L2300/0838 ,  B01L2400/027 ,  B01L2400/0415 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14

    摘要： A device and a method for printing biomolecules onto a substrate using an electrohydrodynamic (EHD) effect are provided. The device for printing biomolecules onto a substrate using an electrohydrodynamic (EHD) effect includes at least one capillary having an outlet through which a feeding solution of biomolecules selected from the group consisting of nucleic acids, proteins, and oligopeptides is discharged, the nucleic acids being selected from the group consisting of probe DNA, RNA, peptide nucleic acid (PNA), and LNA and the proteins being selected from the group consisting of antigen and antibody; a printer body supporting the at least one capillary; a substrate below the outlet having a target surface onto which the biomolecules are deposited; a first electric field forming electrode located on the printer body around the circumference of the outlet; a second electric field forming electrode spaced apart from the first electrode by a predetermined distance; and a voltage applying unit which is electrically connected to the first electrode and the second electrode to apply an alternating current (AC) voltage between the first electrode and the second electrode so that an electric field may be formed around the biomolecule solution suspended in the outlet, and due to the interaction of the electric field and a difference in dielectric constant between the biomolecule solution having a free surface and the surrounding atmosphere, the electric force acts inward on the biomolecule solution from the surroundings, thereby dropping a predetermined amount of the biomolecule solution onto the target surface of the substrate. By using an EHD effect, the advantages of the device and method are that the process is quick, the biomolecule spots are uniform and easily aligned, and especially it is possible to spot the biomolecules correctly and reproducibly even when the printer body is not fully aligned with the substrate. In addition, the device and method are applicable to proteins which are sensitive to heat and biomolecules which have neutral charges.