利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

17 条记录 (耗时 0.029 秒)

    Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images
    9.
    发明授权
    Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images 有权
    使用多个照明方案识别感兴趣的对象，并在相应的多个图像中发现特征的重叠

    公开(公告)号：US06259807B1

    公开(公告)日：2001-07-10

    申请号：US09443949

    申请日：1999-11-19

    申请人： Ilya Ravkin

    发明人： Ilya Ravkin

    IPC分类号： G06K900

    CPC分类号： G06K9/00127 ,  G01N15/1475 ,  G01N2015/0073 ,  G01N2015/1006 ,  G02B21/086 ,  G02B21/16 ,  G02B21/365

    摘要： Detection of fetal nucleated red blood cells (NRBCs) is achieved by employing a combination of brightfield and fluorescence images of nuclear and cytoplasmic markers. The brightfield and fluorescence images are all obtained with a single multibandpass dichroic mirror. The objects in the sample are stained with a fluorescent dye that selectively stains nuclei and a dye that selectively stains fetal hemoglobin in the cytoplasm of fetal RBCs. UV excitation provides fluorescent emissions from the stained cell nuclei and visible illumination provides brightfield transmission of light that is absorbed by the stained cytoplasm. The images are processed to determine regions where the fluorescent emissions by cell nuclei in response to the UV excitation and the absorption by fetal hemoglobin of the brightfield illumination overlap or are in close proximity. The brightfield and fluorescence images may be sequentially acquired or derived from a single image where the UV excitation and visible illumination occur simultaneously.

    摘要翻译： 通过使用核和细胞质标记物的明场和荧光图像的组合来实现胎儿有核红细胞（NRBC）的检测。 明场和荧光图像均用单个多波束分色镜获得。 样本中的物体用选择性染色核的荧光染料和在胎儿RBC细胞质中选择性地染色胎儿血红蛋白的染料染色。 UV激发提供来自染色的细胞核的荧光发射，并且可见光照提供被染色的细胞质吸收的光的明场透射。 处理图像以确定响应于UV激发的细胞核的荧光发射和亮场照射的胎儿血红蛋白的吸收重叠或紧密接近的区域。 亮场和荧光图像可以从UV激发和可见光同时发生的单一图像顺序地获得或导出。