公开(公告)号：US4642291A

公开(公告)日：1987-02-10

申请号：US413861

申请日：1982-09-01

申请人： J. Gregory Cairncross ,  M. Jules Mattes ,  H. Richard Beresford ,  Anthony P. Albino ,  Alan N. Houghton ,  Kenneth O. Lloyd ,  Lloyd J. Old

发明人： J. Gregory Cairncross ,  M. Jules Mattes ,  H. Richard Beresford ,  Anthony P. Albino ,  Alan N. Houghton ,  Kenneth O. Lloyd ,  Lloyd J. Old

IPC分类号： C07K16/30 ,  C12N5/00 ,  C12N15/00 ,  C12P21/00 ,  C12R1/91

CPC分类号： C07K16/30 ,  Y10S435/948

摘要： Method of forming an antibody producing hybridoma cell line by fusing a myeloma cell line with splenocytes derived from BALB/c mice immunized with human astrocytoma tumor cells, the hybridoma cell line formed, and the monoclonal antibodies generated by said hybridoma cell line. A method of phenotyping astrocytoma tumor cells comprising determining the reaction of said cells to various monoclonal antibodies to astrocytoma tumor cells is also provided.

摘要翻译： 通过将骨髓瘤细胞系与源自用人星形细胞瘤肿瘤细胞免疫的BALB / c小鼠的脾细胞，形成的杂交瘤细胞系和由所述杂交瘤细胞系产生的单克隆抗体融合来形成产生抗体的杂交瘤细胞系的方法。 还提供了表型星形细胞瘤肿瘤细胞的方法，包括测定所述细胞与星形细胞瘤肿瘤细胞的各种单克隆抗体的反应。

公开(公告)号：US4562160A

公开(公告)日：1985-12-31

申请号：US482153

申请日：1983-04-01

申请人： Francisco X. Real ,  M. Jules Mattes ,  Alan N. Houghton ,  Philip O. Livingston ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old

发明人： Francisco X. Real ,  M. Jules Mattes ,  Alan N. Houghton ,  Philip O. Livingston ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old

IPC分类号： C07K16/30 ,  A61K39/395 ,  A61K39/44 ,  A61K43/00

CPC分类号： C07K16/3053 ,  Y10S435/948 ,  Y10S435/961 ,  Y10S436/804 ,  Y10S436/813 ,  Y10S436/815 ,  Y10S436/823 ,  Y10S530/806 ,  Y10S530/807 ,  Y10S530/828

摘要： The present invention concerns novel immunoprecipitating autologous antibodies which recognize the Class 1 gp90 antigen on melanoma cells. These antibodies, optionally tagged with a chromophoric or radioactive label and immobilized on an inert support, may be used to recognize and isolate the gp90 antigen from melanoma cell extracts. Monoclonal antibodies to melanoma may be screened with the gp90 antigen for those which recognize epitopes other than the FD antigenic system.The cell line containing the gp90 antigen which has been cultured in vitro is a source of gp90 antigen for generation of monoclonal antibodies which will be useful in analyzing the gp90 antigen for those epitopes which may be of diagnostic value in immunoassay of melanoma.

摘要翻译： 本发明涉及识别黑素瘤细胞上的1类gp90抗原的新型免疫沉淀自体抗体。 可以使用任选地标记有发色或放射性标记并固定在惰性载体上的这些抗体来识别和分离来自黑素瘤细胞提取物的gp90抗原。 可以用gp90抗原筛选黑素瘤单克隆抗体，以识别除FD抗原系统以外的表位。 含有体外培养的gp90抗原的细胞系是用于产生单克隆抗体的gp90抗原的来源，其可用于分析可能在黑素瘤免疫测定中具有诊断价值的那些表位的gp90抗原。

公开(公告)号：US4798790A

公开(公告)日：1989-01-17

申请号：US756362

申请日：1985-07-18

申请人： Timothy M. Thomson ,  M. Jules Mattes ,  Lloyd J. Old ,  Kenneth O. Lloyd ,  Linda Roux

发明人： Timothy M. Thomson ,  M. Jules Mattes ,  Lloyd J. Old ,  Kenneth O. Lloyd ,  Linda Roux

IPC分类号： G01N33/574 ,  A61K39/395 ,  C07K14/005 ,  C07K14/195 ,  C07K16/00 ,  C07K16/18 ,  C07K16/30 ,  C12N5/00 ,  C12N5/10 ,  C12N15/00 ,  C12N15/02 ,  C12P21/00 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577 ,  G01N33/53 ,  A61K39/00

CPC分类号： C07K16/18 ,  Y10S436/813 ,  Y10S530/808

摘要： Monoclonal antibody TA99, which specifically binds to a pigmentation associated antigen present on melanoma cells is described. Additionally, the hybridoma cell line deposited with the ATCC under Accession Number HB 8704 from which the antibody is derived, as well as methods for using the antibody are described.

摘要翻译： 描述了特异性结合存在于黑素瘤细胞上的色素沉着相关抗原的单克隆抗体TA99。 另外，描述了保藏于ATCC的保藏号HB 8704的ATCC的杂交瘤细胞系以及抗体的使用方法。

公开(公告)号：US4591572A

公开(公告)日：1986-05-27

申请号：US481379

申请日：1983-04-01

申请人： M. Jules Mattes ,  Timothy M. Thomson ,  Lloyd J. Old ,  Kenneth O. Lloyd

发明人： M. Jules Mattes ,  Timothy M. Thomson ,  Lloyd J. Old ,  Kenneth O. Lloyd

IPC分类号： A61K39/00 ,  G01N33/532 ,  G01N33/574

CPC分类号： G01N33/532 ,  G01N33/5743 ,  A61K39/00 ,  Y10S435/948 ,  Y10S435/96 ,  Y10S436/804 ,  Y10S436/811 ,  Y10S436/813 ,  Y10S436/815 ,  Y10S436/823 ,  Y10S436/828 ,  Y10S530/806

摘要： The present invention concerns an autologous precipitating antibody and the gp70 pigment-associated antigen on melanoma cells which it recognizes. The antibody is useful in detecting pigmented melanoma cells in excised specimen, serum or urine.

摘要翻译： 本发明涉及其识别的黑色素瘤细胞上的自体沉淀抗体和gp70颜料相关抗原。 该抗体可用于检测切除的标本，血清或尿液中的色素沉着黑素瘤细胞。

公开(公告)号：US4666845A

公开(公告)日：1987-05-19

申请号：US562465

申请日：1983-12-16

申请人： M. Jules Mattes ,  John L. Lewis, Jr. ,  Kenneth O. Lloyd ,  Lloyd J. Old ,  Carlos Cordon-Cardo

发明人： M. Jules Mattes ,  John L. Lewis, Jr. ,  Kenneth O. Lloyd ,  Lloyd J. Old ,  Carlos Cordon-Cardo

IPC分类号： G01N33/574 ,  A61K39/395 ,  C07K14/005 ,  C07K14/195 ,  C07K16/00 ,  C07K16/30 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577 ,  C12N5/00 ,  C12N15/00 ,  G01N33/53

CPC分类号： C07K16/30 ,  Y10S435/948 ,  Y10S436/808 ,  Y10S436/813 ,  Y10S530/808 ,  Y10S530/864

摘要： Mouse monoclonal antibodies to several cell antigens of human ovarian, cervical and endometrial carcinomas have been produced and characterized. The distribution of the antigens was determined by mixed hemagglutination assays on 153 normal and malignant cell cultures of various types, and by immunoperoxidase staining of frozen sections of 27 normal adult and 24 fetal tissues. five monoclonal antibodies representative of five classes of mAb raised to restricted ovarian, cervical and endometrial cells were tested extensively producing mAb reactive with cancer but not normal cells. One such mAb, MF116 was readily detected in the spent culture medium of metabolically radiolabeled cells. These antibodies, reacting with relatively restricted cell surface antigens, are useful in the analysis of epithelial cell differentiation, in cancer diagnosis and therapy and in tissue typing of normal or abnormal cells.

摘要翻译： 人类卵巢癌，子宫颈癌和子宫内膜癌的几种细胞抗原的小鼠单克隆抗体已被制备和表征。 通过混合血细胞凝集测定法对各种类型的153种正常和恶性细胞培养物进行抗原分布，通过免疫过氧化物酶染色法检测27例正常成人和24例胎儿组织的冷冻切片。 代表五类单克隆抗体的五种单克隆抗体阳性细胞被广泛地测试产生与癌症但不是正常细胞反应的mAb。 在代谢放射性标记的细胞的废培养基中容易检测到一种这样的mAb，MF116。 这些抗体与相对受限的细胞表面抗原反应可用于分析上皮细胞分化，癌症诊断和治疗以及正常或异常细胞的组织分型。

公开(公告)号：US5143843A

公开(公告)日：1992-09-01

申请号：US732724

申请日：1991-07-18

申请人： M. Jules Mattes ,  Lloyd J. Old ,  Kenneth O. Lloyd

发明人： M. Jules Mattes ,  Lloyd J. Old ,  Kenneth O. Lloyd

IPC分类号： A61K38/00 ,  C07K16/30 ,  C12N5/20

CPC分类号： C07K16/30 ,  A61K38/00

摘要： Monoclonal antibodies to human antigens present on a majority of human cells are described. These mAbs have use in a method for isolating mAb for less expressive antigens, such as cancer antigens, or other antigens associated with particular abnormalities, disorders or disease state. The latter mAbs may be weaker than or not present to such an extent as the first mentioned mAbs. For example, these less expressive mAbs would be useful for cancer diagnosis, especially in the early stages, and for cancer treatment as well where the cancer cell is the target cell for the mAb. The mAb can be tagged with a tissue destructive agent such as a radio-label, a toxin, a chemical poisen, and the like. Some of the mAbs described, subset tumors of particular types and so are useful for tumor subclassification. The mAbs described are also useful in analyzing the properties and functions of their respective antigens in human cells.

摘要翻译： 描述存在于大多数人细胞上的人抗原的单克隆抗体。 这些mAb用于分离用于较少表达性抗原（例如癌症抗原）或与特定异常，病症或疾病状态相关的其它抗原的mAb的方法。 后一种mAb可能弱于或不存在至如前述mAb的程度。 例如，这些较少表达的mAb可用于癌症诊断，特别是在早期阶段以及用于癌症治疗以及其中癌细胞是mAb的靶细胞。 mAb可以用组织破坏剂如放射标签，毒素，化学品等标记。 描述的一些mAb，特定类型的子集，因此可用于肿瘤分类。 所述的mAb也可用于分析其各自抗原在人细胞中的性质和功能。

公开(公告)号：US4863854A

公开(公告)日：1989-09-05

申请号：US764862

申请日：1985-08-12

申请人： M. Jules Mattes ,  John L. Lewis, Jr. ,  Lloyd J. Old ,  Kenneth O. Floyd ,  Katherine Look

发明人： M. Jules Mattes ,  John L. Lewis, Jr. ,  Lloyd J. Old ,  Kenneth O. Floyd ,  Katherine Look

IPC分类号： G01N33/574 ,  A61K39/395 ,  C07K14/005 ,  C07K14/195 ,  C07K14/435 ,  C07K16/00 ,  C07K16/30 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577

CPC分类号： C07K16/30 ,  Y10S436/813 ,  Y10S530/828 ,  Y10S530/844 ,  Y10S530/864

摘要： Three new monoclonal antibodies, MU78, MT334, and MQ49, and the hybridoma cell lines producing these, are disclosed. The antibodies specifically bind to mucin-like antigens with distribution over various carcinomas.

摘要翻译： 公开了三种新的单克隆抗体MU78，MT334和MQ49以及产生这些的杂交瘤细胞系。 抗体特异性结合粘蛋白样抗原，分布在各种癌上。

公开(公告)号：US5759514A

公开(公告)日：1998-06-02

申请号：US695182

申请日：1996-08-01

申请人： M. Jules Mattes

发明人： M. Jules Mattes

IPC分类号： A61K38/00 ,  A61K39/395 ,  A61K51/00 ,  A61K51/08 ,  A61K51/10 ,  A61P35/00 ,  A61M36/14

CPC分类号： A61K51/1093 ,  A61K51/0421 ,  A61K2121/00

摘要： A conjugate of a tumor cell-targeting protein or polypeptide and a nucleic acid-targeting small molecule labeled with an Auger electron-emitting radionuclide is useful for tumor therapy. The tumor cell-targeting protein or polypeptide may be an antibody or fragment thereof, a hormone or a growth factor.

摘要翻译： 肿瘤细胞靶向蛋白或多肽与用俄歇电子发射放射性核素标记的核酸靶向小分子的缀合物可用于肿瘤治疗。 肿瘤细胞靶向蛋白或多肽可以是抗体或其片段，激素或生长因子。

公开(公告)号：US5059520A

公开(公告)日：1991-10-22

申请号：US900820

申请日：1986-08-27

申请人： Koichi Furukawa ,  Hanrik Clausen ,  Sen-itiroh Hakomori ,  Junichi Sakamoto ,  Katherine Look ,  M. Jules Mattes ,  Lloyd Kenneth O.

发明人： Koichi Furukawa ,  Hanrik Clausen ,  Sen-itiroh Hakomori ,  Junichi Sakamoto ,  Katherine Look ,  M. Jules Mattes ,  Lloyd Kenneth O.

IPC分类号： C07K16/30 ,  C07K16/34 ,  G01N33/574 ,  G01N33/80

CPC分类号： C07K16/30 ,  C07K16/34 ,  G01N33/574 ,  G01N33/57469 ,  G01N33/80 ,  G01N2333/705 ,  Y10S436/811 ,  Y10S436/813

摘要： Monoconal antibodies HT29-36, CB, CLH6, M2, and S12 are useful in determining the presence of Type A blood group antigen and in distinguishing different forms of this antigen. As type A antigen exhibits several forms differing in minor respects only, the monoclonal antibodies are useful in making fine distinctions between the different forms, and may be used, e.g., in cancer diagnosis, and the like.

摘要翻译： 单克隆抗体HT29-36，CB，CLH6，M2和S12可用于确定A型血型抗原的存在和区分该抗原的不同形式。 由于A型抗原仅在少数方面表现出几种形式，所以单克隆抗体可用于在不同形式之间进行细微区分，并且可用于例如癌症诊断等。