-
公开(公告)号：US20100323452A1
公开(公告)日：2010-12-23
申请号：US12528304
申请日：2007-02-22
CPC分类号： C08F8/44 , B01D15/361 , B01J47/12 , C08F6/003 , C08G65/30 , C08G65/3326 , C08G65/3346 , G01N30/02 , Y10T436/255
摘要： A process for purification of water soluble polymers is provided. A polymer of interest can be separated from a mixture of polymers, provided the polymer of interest differs from other polymers in the mixture in the number of reactive terminal groups. The process involves derivatizing polymers at the reactive terminal groups with a derivatizing molecule, bearing either (i) two or three cationic or anionic ionizable groups, and a group capable of covalent bonding to the reactive terminal groups or (ii) three or four ionizable groups, at least one of which is capable of covalent bonding to the reactive terminal groups; followed by ion exchange. The process allows removal of PEG from MPEG, and can be used for polymers having an average size greater than 9,000 Da Derivatization may be reversible in order to allow re-use of recovered contaminant polymers.
摘要翻译： 提供了一种纯化水溶性聚合物的方法。 感兴趣的聚合物可以从聚合物的混合物中分离出来，只要目的聚合物与反应性末端基团数目的混合物中的其它聚合物不同。 该方法包括用衍生化分子衍生化反应性末端基团的聚合物，该衍生化分子带有（i）两个或三个阳离子或阴离子可离子基团，以及能够与反应性末端基团共价连接的基团，或（ii）三个或四个可离子化基团 ，其中至少一个能够与反应性末端基团共价结合; 然后进行离子交换。 该方法允许从MPEG去除PEG，并且可以用于平均尺寸大于9,000Da的聚合物。衍生化可以是可逆的，以便允许再利用回收的污染物聚合物。
-
公开(公告)号：US08222039B2
公开(公告)日：2012-07-17
申请号：US12528304
申请日：2007-02-22
IPC分类号： C08G65/329
CPC分类号： C08F8/44 , B01D15/361 , B01J47/12 , C08F6/003 , C08G65/30 , C08G65/3326 , C08G65/3346 , G01N30/02 , Y10T436/255
摘要： A process for purification of water soluble polymers is provided. A polymer of interest can be separated from a mixture of polymers, provided the polymer of interest differs from other polymers in the mixture in the number of reactive terminal groups. The process involves derivatizing polymers at the reactive terminal groups with a derivatizing molecule, bearing either (i) two or three cationic or anionic ionizable groups, and a group capable of covalent bonding to the reactive terminal groups or (ii) three or four ionizable groups, at least one of which is capable of covalent bonding to the reactive terminal groups; followed by ion exchange. The process allows removal of PEG from MPEG, and can be used for polymers having an average size greater than 9,000 Da Derivatization may be reversible in order to allow re-use of recovered contaminant polymers.
摘要翻译： 提供了一种纯化水溶性聚合物的方法。 感兴趣的聚合物可以从聚合物的混合物中分离出来，只要目的聚合物与反应性末端基团数目的混合物中的其它聚合物不同。 该方法包括用衍生化分子衍生化反应性末端基团的聚合物，该衍生化分子带有（i）两个或三个阳离子或阴离子可离子基团，以及能够与反应性末端基团共价连接的基团，或（ii）三个或四个可离子化基团 ，其中至少一个能够与反应性末端基团共价结合; 然后进行离子交换。 该方法允许从MPEG去除PEG，并且可以用于平均尺寸大于9,000Da的聚合物。衍生化可以是可逆的，以便允许再利用回收的污染物聚合物。
-
公开(公告)号：US20110124841A1
公开(公告)日：2011-05-26
申请号：US12747653
申请日：2008-12-12
申请人： James F. Monthony , Paul Xiang-Qin Liu , Li Yang , Kaisong Zhou
发明人： James F. Monthony , Paul Xiang-Qin Liu , Li Yang , Kaisong Zhou
CPC分类号： C12N15/1027 , C07K1/1075 , C07K1/1077 , C07K14/00 , C12N15/63
摘要： The present invention relates to a method of preparing modified polypeptides, by linking a target polypeptide to a carrier molecule that is designed to bear one or more water-soluble polymer molecules, via protein trans-splicing. The polymer molecules can be attached to the carrier molecule either before or after ligation to the target polypeptide. Novel protein trans-splicing elements (known as “split inteins”) and trans-splicing partners are also provided.
摘要翻译： 本发明涉及通过蛋白质转接将目标多肽与被设计为承载一种或多种水溶性聚合物分子的载体分子连接来制备修饰的多肽的方法。 聚合物分子可以在与靶多肽连接之前或之后连接至载体分子。 还提供了新的蛋白质转接元件（称为“分裂内含肽”）和转拼合子。
-
公开(公告)号：US4749655A
公开(公告)日：1988-06-07
申请号：US56081
申请日：1987-06-01
CPC分类号： C12M45/22 , C12M33/02 , A61B10/0096
摘要： A self-contained specimen collecting and transporting unit is provided which has all of the advantages of the known specimen collecting and transporting units and which is characterized by the ability to preserve microorganisms during the transporting thereof in the transporting unit. The improved specimen collecting and transporting unit has a pledget composed of carbon fiber. The use of a carbon fiber pledget has been found to have a synergistic effect in preserving the viability of microorganisms which are obtained by use of the swab of the specimen collecting and transporting unit.
摘要翻译： 提供了一种独立的样品收集和输送单元，其具有已知的样品收集和输送单元的所有优点，其特征在于在运输单元期间保存微生物的能力。 改进的标本收集和输送单元具有由碳纤维组成的纤维。 已经发现使用碳纤维糊剂在保持通过使用样品收集和输送单元的拭子获得的微生物的活力方面具有协同效应。
-
公开(公告)号：US5567598A
公开(公告)日：1996-10-22
申请号：US25899
申请日：1993-03-03
CPC分类号： C12Q1/04 , C12Q1/18 , C12Q2304/44 , Y10S435/968 , Y10S436/80
摘要： This invention presents methods for detection and evaluation of metabolic activity of microorganisms based upon their ability to consume dissolved oxygen. The methods utilize a fluorescence detection system which makes use of the sensitivity of the fluorescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's fluorescent emission in a concentration dependent manner. Respiring microorganisms will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification and metabolic activity of microorganisms by determining their effect on the oxygen concentration of the media in which they are present.
摘要翻译： 本发明提供了基于其消耗溶解氧的能力来检测和评估微生物的代谢活性的方法。 该方法利用荧光检测系统，其利用某些化合物的荧光发射对氧的存在的灵敏度，其以浓度依赖性方式猝灭（减少）化合物的荧光发射。 呼吸微生物将影响浸入其中的液体介质的氧浓度。 因此，本发明提供了一种方便的系统，通过确定它们对存在的微生物的存在，鉴定和代谢活性的影响，其对它们所存在的培养基的氧浓度的影响。
-
6.发明授权Multiple aliquot device for distributing a liquid solution into a well 失效用于将液体溶液分配到一个良好的多个ALIQUOT装置
公开(公告)号：US5182082A
公开(公告)日：1993-01-26
申请号：US644786
申请日：1991-01-23
CPC分类号： B01L3/5025 , Y10T137/85938
摘要： The present invention is a device which allows for receiving, distributing and storing a sample into numerous aliquots of small volume without air entrapment and with retention of aliquots when the device is manipulated. The device allows for the treatment of any or all of the aliquots with the same or different reagents and/or other chemical additives. The device comprises a housing for containing a body for guiding a sample into a plurality of wells without the need for pipetting aids, without multiple manipulations, without the retention of air and for retaining sample in the wells.
-
7.发明授权
公开(公告)号：US5338666A
公开(公告)日：1994-08-16
申请号：US860841
申请日：1992-03-31
CPC分类号： B01L3/5025 , Y10T137/85938
摘要： A method for receiving, distributing and storing a sample into numerous aliquots of small volume without air entrapment and with retention of aliquots when the device is manipulated and for the treatment of any or all of the aliquots with the same or different reagents and/or other chemical additives.
摘要翻译： 一种用于接收，分配和储存样品至少体积的等分试样而没有空气滞留并且当装置被操纵时保留等分试样并用相同或不同试剂和/或其它方法处理任何或所有等分试样的方法 化学添加剂。
-
公开(公告)号：US5313959A
公开(公告)日：1994-05-24
申请号：US50521
申请日：1993-04-21
CPC分类号： B67B7/92 , A61B10/0096 , C12M45/22 , G01N1/02 , A61F13/38 , G01N1/31 , G01N2001/028
摘要： The present invention is a device and method for breaking an ampoule. In particular, the device is preferably for use in an apparatus for collecting and transporting biological specimens. The device comprises a guard arrangement that surrounds a frangible ampoule. With appropriate force applied to the guard arrangement, the frangible ampoule is ruptured.
摘要翻译： 本发明是用于破坏安瓿的装置和方法。 特别地，该装置优选用于收集和运输生物样品的装置中。 该装置包括围绕易碎安瓿的防护装置。 通过适当的力量施加到防护装置上，易碎的安瓿破裂。
-
公开(公告)号：USD355970S
公开(公告)日：1995-02-28
申请号：US10072
申请日：1993-06-28
-
10.发明授权
公开(公告)号：US5163441A
公开(公告)日：1992-11-17
申请号：US627295
申请日：1990-12-14
CPC分类号： A61B10/02 , A61F13/38 , C12M33/02 , C12M45/22 , A61B10/0096 , G01N2001/028
摘要： A microbiological culture collection and transport device maintains viable organisms for periods of time longer than possible with existing sampling devices. It also allows recovery of detectable antigen at levels not achievable with conventional swabs. The new device has a sterile swabbing tip made with a non-toxic polyurethane foam having open cells at its exposed surface. It does not require a transport medium and can be used dry. The device may further include a sample inoculator to distribute organisms collected onto a solid or semi solid medium. Methods for collecting and transporting microbiological specimens and for recovering detectable antigen are also described.
摘要翻译： 微生物培养物收集和运输装置在现有采样装置的时间长的时间内维持活生物体。 它还允许以常规拭子不能达到的水平回收可检测的抗原。 新装置具有无菌拭子尖端，其在其暴露表面上具有开放细胞的无毒聚氨酯泡沫塑料制成。 它不需要运输介质，可以使用干燥。 该装置可以进一步包括样品接种器以将收集的生物体分布到固体或半固体培养基上。 还描述了收集和运送微生物标本和恢复可检测抗原的方法。
-
-
-
-
-
-
-
-
-
搜索结果
20 条记录 (耗时 0.027 秒)