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    Method, system and reagents for DNA sequencing
    2.
    发明授权
    Method, system and reagents for DNA sequencing 失效
    用于DNA测序的方法，系统和试剂

    公开(公告)号：US5242796A

    公开(公告)日：1993-09-07

    申请号：US780346

    申请日：1991-10-22

    申请人： James M. Prober ,  Rudy J. Dam ,  Charles W. Robertson, Jr ,  Frank W. Hobbs, Jr. ,  George L. Trainor

    发明人： James M. Prober ,  Rudy J. Dam ,  Charles W. Robertson, Jr ,  Frank W. Hobbs, Jr. ,  George L. Trainor

    IPC分类号： C07D405/04 ,  C07D405/12 ,  C07D405/14 ,  C07D471/04 ,  C07F9/6512 ,  C07F9/6558 ,  C07F9/6561 ,  C07H19/06 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/68 ,  G01N27/447

    CPC分类号： C07D405/04 ,  C07D405/12 ,  C07D405/14 ,  C07D471/04 ,  C07F9/65121 ,  C07F9/65586 ,  C07F9/6561 ,  C07H19/06 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/6869 ,  G01N27/44721 ,  G01N27/44726

    摘要： A DNA sequencing system and method are described to detect the presence of radiant energy emitted from different excited reporter dye-labeled species (DNA fragments) following separation in time and/or space, and the identity of the species which emit radiant energy closely spaced in wavelength. Functions of the emitted energy are obtained which vary over the wavelengths of the closely spaced spectra in different senses and the functions ratioed, whereby the ratio is indicative of the identity of the DNA fragments.The emitting portion of the reporter-labeled DNA fragment is preferably one of a family of fluorescent dyes based on 9-carboxyethyl-6-hydroxy-3-oxo-3 H-xanthene. These xanthene dyes are covalently attached to the DNA fragments through the carboxylic acid functionality, preferably via an amide linkage. The dyes may be protected by including an alkoxy group at the 9-position. A spacer may be inserted between the dye and the amine. The fluorescent dye preferably is attached to the DNA chain terminators and provides many advantages. Thus only DNA sequencing fragments resulting from bona fide termination events will carry a reporter. The DNA sequencing may also be labeled using the xanthene dyes which have general utility as fluorescent labels. Also acyclonucleoside triphosphates are described as being useful as chain terminators in DNA sequencing using a modification of the Sanger method.

    摘要翻译： 描述了DNA测序系统和方法以检测在时间和/或空间分离之后从不同激发的报道染料标记物种（DNA片段）发射的辐射能的存在，以及发射辐射能的物质的身份 波长。 获得发射能量的功能，其在不同感官中的紧密间隔的光谱的波长上变化，并且功能的比例，其中该比率指示DNA片段的身份。 报道分子标记的DNA片段的发射部分优选为基于9-羧基乙基-6-羟基-3-氧代-3H-呫吨的荧光染料族之一。 这些呫吨染料通过羧酸官能团共价连接到DNA片段上，优选通过酰胺键连接。 可以通过在9位包括烷氧基来保护染料。 可以在染料和胺之间插入间隔物。 荧光染料优选连接到DNA链终止剂上并提供许多优点。 因此，只有真正终止事件导致的DNA测序片段才会携带记者。 DNA测序也可以使用具有通用作为荧光标记的呫吨染料进行标记。 也称为二环磷酸核苷三磷酸可用作DNA测序中的连锁终止子，使用Sanger方法的修饰。

    Method of determining small concentrations of chemical compounds by plasma chromatography
    7.
    发明授权
    Method of determining small concentrations of chemical compounds by plasma chromatography 失效
    通过血浆色谱法测定化合物浓度小的方法

    公开(公告)号：US4259573A

    公开(公告)日：1981-03-31

    申请号：US90914

    申请日：1979-11-05

    申请人： James M. Prober ,  Rudy J. Dam

    发明人： James M. Prober ,  Rudy J. Dam

    IPC分类号： G01N27/64 ,  G01N27/66 ,  G01N33/00 ,  B01D59/44

    CPC分类号： G01N27/622 ,  G01N27/66 ,  G01N33/0026

    摘要： An accurate method of determining by plasma chromatography the concentration of an ionizable gaseous or volatile chemical species A in air or another gaseous medium. Small, known increments of species A plus, optionally, another calibrant species are introduced in turn into the plasma chromatograph together with the unknown sample; and the respective changes of the amplitude or area of a characteristic ion peak of species A in the unknown sample are measured. The second, optical calibrant, which is different from species A, must have similar kinetic characteristics but a different ion mobility from the ion formed by A. By means of this technique, inaccurate readings caused by background concentration variations are significantly reduced.

    摘要翻译： 通过等离子体色谱法测定空气或其他气体介质中可电离的气态或挥发性化学物质A的浓度的精确方法。 随着未知样品的不断增加，A +加上任意另外一种校准物质的增量依次进入血浆色谱仪; 并测量未知样品中物种A的特征离子峰的振幅或面积的相应变化。 与物种A不同的第二种光学校准物必须具有相似的动力学特性，但与A形成的离子具有不同的离子迁移率。通过该技术，由背景浓度变化引起的不准确的读数显着降低。