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11 条记录 (耗时 0.019 秒)

    Method, system and reagents for DNA sequencing
    4.
    发明授权
    Method, system and reagents for DNA sequencing 失效
    用于DNA测序的方法，系统和试剂

    公开(公告)号：US5242796A

    公开(公告)日：1993-09-07

    申请号：US780346

    申请日：1991-10-22

    申请人： James M. Prober ,  Rudy J. Dam ,  Charles W. Robertson, Jr ,  Frank W. Hobbs, Jr. ,  George L. Trainor

    发明人： James M. Prober ,  Rudy J. Dam ,  Charles W. Robertson, Jr ,  Frank W. Hobbs, Jr. ,  George L. Trainor

    IPC分类号： C07D405/04 ,  C07D405/12 ,  C07D405/14 ,  C07D471/04 ,  C07F9/6512 ,  C07F9/6558 ,  C07F9/6561 ,  C07H19/06 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/68 ,  G01N27/447

    CPC分类号： C07D405/04 ,  C07D405/12 ,  C07D405/14 ,  C07D471/04 ,  C07F9/65121 ,  C07F9/65586 ,  C07F9/6561 ,  C07H19/06 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/6869 ,  G01N27/44721 ,  G01N27/44726

    摘要： A DNA sequencing system and method are described to detect the presence of radiant energy emitted from different excited reporter dye-labeled species (DNA fragments) following separation in time and/or space, and the identity of the species which emit radiant energy closely spaced in wavelength. Functions of the emitted energy are obtained which vary over the wavelengths of the closely spaced spectra in different senses and the functions ratioed, whereby the ratio is indicative of the identity of the DNA fragments.The emitting portion of the reporter-labeled DNA fragment is preferably one of a family of fluorescent dyes based on 9-carboxyethyl-6-hydroxy-3-oxo-3 H-xanthene. These xanthene dyes are covalently attached to the DNA fragments through the carboxylic acid functionality, preferably via an amide linkage. The dyes may be protected by including an alkoxy group at the 9-position. A spacer may be inserted between the dye and the amine. The fluorescent dye preferably is attached to the DNA chain terminators and provides many advantages. Thus only DNA sequencing fragments resulting from bona fide termination events will carry a reporter. The DNA sequencing may also be labeled using the xanthene dyes which have general utility as fluorescent labels. Also acyclonucleoside triphosphates are described as being useful as chain terminators in DNA sequencing using a modification of the Sanger method.

    摘要翻译： 描述了DNA测序系统和方法以检测在时间和/或空间分离之后从不同激发的报道染料标记物种（DNA片段）发射的辐射能的存在，以及发射辐射能的物质的身份 波长。 获得发射能量的功能，其在不同感官中的紧密间隔的光谱的波长上变化，并且功能的比例，其中该比率指示DNA片段的身份。 报道分子标记的DNA片段的发射部分优选为基于9-羧基乙基-6-羟基-3-氧代-3H-呫吨的荧光染料族之一。 这些呫吨染料通过羧酸官能团共价连接到DNA片段上，优选通过酰胺键连接。 可以通过在9位包括烷氧基来保护染料。 可以在染料和胺之间插入间隔物。 荧光染料优选连接到DNA链终止剂上并提供许多优点。 因此，只有真正终止事件导致的DNA测序片段才会携带记者。 DNA测序也可以使用具有通用作为荧光标记的呫吨染料进行标记。 也称为二环磷酸核苷三磷酸可用作DNA测序中的连锁终止子，使用Sanger方法的修饰。

    Method and apparatus for remotely detecting gases in the atmosphere
    6.
    发明授权
    Method and apparatus for remotely detecting gases in the atmosphere 失效
    远程检测大气中的气体的方法和装置

    公开(公告)号：US4390785A

    公开(公告)日：1983-06-28

    申请号：US220997

    申请日：1980-12-29

    申请人： Mark E. Faulhaber ,  James M. Prober

    发明人： Mark E. Faulhaber ,  James M. Prober

    IPC分类号： G01J5/60 ,  G01M3/38 ,  G01N21/31 ,  G01N21/35 ,  G01N33/00 ,  G01V9/00 ,  H01J31/49 ,  G01J1/00

    CPC分类号： G01J5/60 ,  G01M3/38 ,  G01N21/3504 ,  G01N33/0047 ,  G01V9/007 ,  G01N21/314 ,  G01N21/35

    摘要： Presence and location of infrared radiation-absorbing or emitting gases in the atmosphere can be ascertained by means of an infrared imaging-analyzing means which views a given scene and receives infrared radiation therefrom. Analytic and reference beams are produced, the latter having reduced sensitivity to the gas of interest, and are converted to electric signals, which are processed in real time to provide a signal corresponding to their ratio. This ratio signal is further processed to generate an image, which can be displayed and viewed. This technique is particularly suitable for surveying large areas for seepage of methane or other hydrocarbon gases from underground gas and/or oil deposits.

    摘要翻译： 通过红外成像分析装置可以确定大气中红外辐射吸收或发射气体的存在和位置，该装置观察给定场景并从其接收红外辐射。 产生分析和参考光束，后者对感兴趣的气体的灵敏度降低，并被转换成电信号，这些信号被实时处理以提供对应于它们的比率的信号。 该比率信号被进一步处理以产生可以显示和观看的图像。 该技术特别适用于大面积测量地下气体和/或油沉积物中甲烷或其他烃类气体的渗漏。