公开(公告)号：US20120034648A1

公开(公告)日：2012-02-09

申请号：US12867927

申请日：2009-03-05

申请人： Paul Klaassen ,  Jan Metske Van Der Laan ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom

发明人： Paul Klaassen ,  Jan Metske Van Der Laan ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom

IPC分类号： C12P37/00 ,  C12N1/14 ,  C12N1/15 ,  C12N1/19 ,  C12P1/00 ,  C12P7/06 ,  C12P7/16 ,  C12P7/56 ,  C12P7/52 ,  C12P7/54 ,  C12P7/48 ,  C12P7/46 ,  C12P7/44 ,  C12P13/04 ,  C12P7/18 ,  C12P7/20 ,  C12P35/00 ,  C12N1/16

CPC分类号： C12N9/92 ,  C12P7/10 ,  Y02E50/16

摘要： The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least about 70% sequence identity to the amino acid sequence set out in SEQ ID NO: 3 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.

摘要翻译： 本发明涉及包含编码木糖异构酶的核苷酸序列的细胞，其中木糖异构酶的氨基酸序列与SEQ ID NO：3所示的氨基酸序列具有至少约70％的序列同一性，其中所述核苷酸 序列与宿主异源。 本发明的细胞可用于生产发酵产物如乙醇的方法中。 这样的方法可以包括用本发明的细胞发酵含有木糖来源的培养基，使得细胞向发酵产物发酵木糖。

公开(公告)号：US08399215B2

公开(公告)日：2013-03-19

申请号：US12867927

申请日：2009-03-05

申请人： Paul Klaassen ,  Jan Metske Van Der Laan ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom

发明人： Paul Klaassen ,  Jan Metske Van Der Laan ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom

IPC分类号： C12P1/00 ,  C12N1/00

CPC分类号： C12N9/92 ,  C12P7/10 ,  Y02E50/16

摘要： The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least about 70% sequence identity to the amino acid sequence set out in SEQ ID NO: 3 and wherein the nucleotide sequence is heterologous to the host. The cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.

摘要翻译： 本发明涉及包含编码木糖异构酶的核苷酸序列的细胞，其中木糖异构酶的氨基酸序列与SEQ ID NO：3所示的氨基酸序列具有至少约70％的序列同一性，其中所述核苷酸 序列与宿主异源。 本发明的细胞可以用于生产发酵产物如乙醇的方法。 这样的方法可以包括用本发明的细胞发酵含有木糖来源的培养基，使得细胞向发酵产物发酵木糖。

公开(公告)号：US20130040297A1

公开(公告)日：2013-02-14

申请号：US13642107

申请日：2011-04-19

申请人： Paul Klaassen ,  Bianca Elisabeth Maria Gielesen ,  Wilbert Herman Marie Heijne ,  Gijsberdina Pieternella Van Suylekom

发明人： Paul Klaassen ,  Bianca Elisabeth Maria Gielesen ,  Wilbert Herman Marie Heijne ,  Gijsberdina Pieternella Van Suylekom

IPC分类号： C12N1/19 ,  C12P7/14 ,  C12P7/16 ,  C12P7/04 ,  C12P7/56 ,  C12P7/42 ,  C12P7/40 ,  C12P7/54 ,  C12P7/46 ,  C12P7/44 ,  C12P7/48 ,  C12P13/12 ,  C12P13/22 ,  C12P13/08 ,  C12P13/20 ,  C12P7/18 ,  C12P5/02 ,  C12P7/20 ,  C12P35/00 ,  C12P3/00 ,  C12N9/50 ,  C12N9/42 ,  C12N9/26 ,  C12N9/24 ,  C12N9/20 ,  C12N9/88 ,  C12N9/02 ,  C12N9/10 ,  C12P37/00 ,  C07K14/00 ,  C12Q1/68

CPC分类号： C12P7/10 ,  C07K14/395 ,  C12N1/36 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/10 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/2405 ,  C12N9/2411 ,  C12N9/2437 ,  C12N9/2468 ,  C12N9/248 ,  C12N9/50 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92 ,  C12P5/026 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P13/005 ,  C12P13/08 ,  C12P13/12 ,  C12P13/20 ,  C12P13/227 ,  C12P17/10 ,  C12P35/00 ,  C12Y101/01 ,  C12Y207/01016 ,  C12Y501/03004 ,  C12Y503/01004 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： The invention relates to a process for the production of cells which are capable of converting arabinose, comprising the following steps: a) Introducing into a host strain that cannot convert arabinose, the genes AraA, araB and araD, this cell is designated as constructed cell; b) Subjecting the constructed cell to adaptive evolution until a cell that converts arabinose is obtained, c) Optionally, subjecting the first arabinose converting cell to adaptive evolution to improve the arabinose conversion; the cell produced in step b) or c) is designated as first arabinose converting cell; d) Analysing the full genome or part of the genome of the first arabinose converting cell and that of the constructed cell; e) Identifying single nucleotide polymorphisms (SNP's) in the first arabinose converting cell; and f) Using the information of the SNP's in rational design of a cell capable of converting arabinose; g) Construction of the cell capable of converting arabinose designed in step f).

摘要翻译： 本发明涉及生产能够转化阿拉伯糖的细胞的方法，包括以下步骤：a）引入不能转化阿拉伯糖的宿主菌株，AraA，araB和araD基因，该细胞被命名为构建的细胞 ; b）使构建的细胞进行自适应进化，直到获得转化阿拉伯糖的细胞，c）任选地，使第一阿拉伯糖转化细胞经受适应性进化以改善阿拉伯糖转化; 步骤b）或c）中产生的细胞被指定为第一阿拉伯糖转化细胞; d）分析第一个阿拉伯糖转化细胞和构建的细胞的全基因组或部分基因组; e）鉴定第一个阿拉伯糖转化细胞中的单核苷酸多态性（SNP）; 和f）使用SNP的信息来合理设计能够转化阿拉伯糖的细胞; g）能够转化步骤f）中设计的阿拉伯糖的细胞的构建。

公开(公告)号：US09096675B2

公开(公告)日：2015-08-04

申请号：US13642114

申请日：2011-04-19

申请人： Paul Klaassen ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom ,  Wilbert Herman Marie Heijne

发明人： Paul Klaassen ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom ,  Wilbert Herman Marie Heijne

IPC分类号： C12P7/06 ,  C12N1/20 ,  C12N9/00 ,  C07K14/395 ,  C12N1/36

CPC分类号： C12P7/10 ,  C07K14/395 ,  C12N1/36 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/10 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/2405 ,  C12N9/2411 ,  C12N9/2437 ,  C12N9/2468 ,  C12N9/248 ,  C12N9/50 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92 ,  C12P5/026 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P13/005 ,  C12P13/08 ,  C12P13/12 ,  C12P13/20 ,  C12P13/227 ,  C12P17/10 ,  C12P35/00 ,  C12Y101/01 ,  C12Y207/01016 ,  C12Y501/03004 ,  C12Y503/01004 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.

摘要翻译： 本发明涉及适用于从包含葡萄糖，半乳糖，阿拉伯糖和木糖的糖组合物生产一种或多种发酵产物的细胞，其中所述细胞包含两至十五份一种或多种木糖异构酶基因或两至十五份 一种或多种木糖还原酶和木糖醇脱氢酶，以及两到十个拷贝的araA，araB和araD基因，其中这些基因整合到细胞基因组中。

公开(公告)号：US20140141473A1

公开(公告)日：2014-05-22

申请号：US14112713

申请日：2012-04-20

申请人： Paul Klaassen ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom ,  Panagiotis Sarantinopoulos ,  Wilbert Herman Marie Heijne ,  Aldo Greeve

发明人： Paul Klaassen ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom ,  Panagiotis Sarantinopoulos ,  Wilbert Herman Marie Heijne ,  Aldo Greeve

IPC分类号： C12P7/10

CPC分类号： C12P7/10 ,  C07K14/395 ,  C12N1/36 ,  C12N9/1205 ,  C12N9/90 ,  C12N9/92 ,  C12P2203/00 ,  C12R1/865 ,  C12Y207/01016 ,  C12Y503/01 ,  C12Y503/01003 ,  C12Y503/01005 ,  Y02E50/16

摘要： Yeast cell belonging to the genus Saccharomyces having introduced into its genome at least one xylA gene and at least one of each of araA, araB and araD genes and that is capable of consuming a mixed sugar mixture comprising glucose, xylose and arabinose, wherein the cell co-consumes glucose and arabinose, has genetic variations obtained during adaptive evolution and has a specific xylose consumption rate in the presence of glucose that is 0.25 g xylose/h, g DM or more.

摘要翻译： 属于酵母属的酵母细胞已经在其基因组中引入了至少一种xylA基因和araA，araB和araD基因中的至少一种，并且能够消耗包含葡萄糖，木糖和阿拉伯糖的混合糖混合物，其中细胞 共同消耗葡萄糖和阿拉伯糖，在适应性进化过程中获得遗传变异，并且在葡萄糖存在下具有特异性的木糖消耗率，其为0.25g木糖/ h，g DM或更多。

公开(公告)号：US20120115186A1

公开(公告)日：2012-05-10

申请号：US13383190

申请日：2010-07-06

申请人： Paul Klaassen ,  Gijsberdina Pieternella Van Suylekom ,  Bianca Elisabeth Maria Gielesen ,  Nicolette Jasmijn Broers ,  Beate Wiedemann ,  Wilhelmus Theodorus Antonius Maria De Laat

发明人： Paul Klaassen ,  Gijsberdina Pieternella Van Suylekom ,  Bianca Elisabeth Maria Gielesen ,  Nicolette Jasmijn Broers ,  Beate Wiedemann ,  Wilhelmus Theodorus Antonius Maria De Laat

IPC分类号： C12P1/02 ,  C12P39/00 ,  C12P7/16 ,  C12P7/56 ,  C12P7/42 ,  C12P7/40 ,  C12P7/54 ,  C12P7/46 ,  C12P7/48 ,  C12P13/08 ,  C12P13/12 ,  C12P13/22 ,  C12P13/20 ,  C12P7/18 ,  C12P5/02 ,  C12P7/20 ,  C12P35/00 ,  C12N9/50 ,  C12N9/42 ,  C12N9/26 ,  C12N9/24 ,  C12N9/88 ,  C12N9/20 ,  C12N9/02 ,  C12N9/10 ,  C12N15/113 ,  C12P7/06

CPC分类号： C12P7/10 ,  C12P7/46 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia, and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.

摘要翻译： 本发明涉及从糖组合物生产一种或多种发酵产物的方法，包括以下步骤：a）在属于酵母属，克鲁维酵母属，假丝酵母​​属，毕赤酵母属的酵母存在下发酵糖组合物 ，粟酒裂殖酵母属（Schizosaccharomyces），汉逊酵母（Hansenula），克洛克氏菌属（Schwanniomyces）或耶氏酵母属（Yarrowia））和b）回收发酵产物，其中酵母包含araA，araB和araD基因，糖组合物包含葡萄糖，半乳糖和阿拉伯糖。

公开(公告)号：US09551015B2

公开(公告)日：2017-01-24

申请号：US13383190

申请日：2010-07-06

申请人： Paul Klaassen ,  Gijsberdina Pieternella Van Suylekom ,  Bianca Elisabeth Maria Gielesen ,  Nicolette Jasmijn Broers ,  Beate Wiedemann ,  Wilhelmus Theodorus Antonius Maria De Laat

发明人： Paul Klaassen ,  Gijsberdina Pieternella Van Suylekom ,  Bianca Elisabeth Maria Gielesen ,  Nicolette Jasmijn Broers ,  Beate Wiedemann ,  Wilhelmus Theodorus Antonius Maria De Laat

IPC分类号： C12P1/02 ,  C12P7/10

CPC分类号： C12P7/10 ,  C12P7/46 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps:a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia,: andb) recovery of the fermentation product,wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.

摘要翻译： 其中所述酵母包含基因araA，araB和araD，并且所述糖组合物包含葡萄糖，半乳糖和阿拉伯糖。

公开(公告)号：US20130059331A1

公开(公告)日：2013-03-07

申请号：US13642114

申请日：2011-04-19

申请人： Paul Klaassen ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom ,  Wilbert Herman Marie Heijne

发明人： Paul Klaassen ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom ,  Wilbert Herman Marie Heijne

IPC分类号： C12N1/19 ,  C12P7/16 ,  C12P7/04 ,  C12P7/56 ,  C12P7/42 ,  C12P7/40 ,  C12P7/54 ,  C12P7/46 ,  C12P7/48 ,  C12P13/04 ,  C12P13/08 ,  C12P13/12 ,  C12P13/22 ,  C12P13/20 ,  C12P7/18 ,  C12P5/02 ,  C12P7/20 ,  C12P13/00 ,  C12P35/00 ,  C12P3/00 ,  C12N9/50 ,  C12N9/42 ,  C12N9/26 ,  C12N9/24 ,  C12N9/20 ,  C12N9/88 ,  C12N9/02 ,  C12N9/10 ,  C12P7/14

CPC分类号： C12P7/10 ,  C07K14/395 ,  C12N1/36 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/10 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/2405 ,  C12N9/2411 ,  C12N9/2437 ,  C12N9/2468 ,  C12N9/248 ,  C12N9/50 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92 ,  C12P5/026 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P13/005 ,  C12P13/08 ,  C12P13/12 ,  C12P13/20 ,  C12P13/227 ,  C12P17/10 ,  C12P35/00 ,  C12Y101/01 ,  C12Y207/01016 ,  C12Y501/03004 ,  C12Y503/01004 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.

公开(公告)号：US09701972B2

公开(公告)日：2017-07-11

申请号：US14236191

申请日：2012-08-02

申请人： Paul Klaassen ,  Gijsberdina Pieternella Van Suylekom ,  Rene Marcel De Jong

发明人： Paul Klaassen ,  Gijsberdina Pieternella Van Suylekom ,  Rene Marcel De Jong

IPC分类号： C12N15/81 ,  C12N9/92 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P5/02 ,  C12P7/06 ,  C12P35/00

CPC分类号： C12N15/81 ,  C12N9/92 ,  C12N15/815 ,  C12P5/026 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P35/00 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17

摘要： The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least 75% sequence identity to the amino acid sequence set out in SEQ ID NO: 2 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.

公开(公告)号：US20140162312A1

公开(公告)日：2014-06-12

申请号：US14236191

申请日：2012-08-02

申请人： Paul Klaassen ,  Gijsberdina Pieternella Van Suylekom ,  Rene Marcel De Jong

发明人： Paul Klaassen ,  Gijsberdina Pieternella Van Suylekom ,  Rene Marcel De Jong

IPC分类号： C12N15/81 ,  C12P7/16 ,  C12P7/56 ,  C12P7/42 ,  C12P7/40 ,  C12P35/00 ,  C12P7/46 ,  C12P7/48 ,  C12P7/18 ,  C12P5/02 ,  C12P7/20 ,  C12P7/06 ,  C12P7/54

CPC分类号： C12N15/81 ,  C12N9/92 ,  C12N15/815 ,  C12P5/026 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P35/00 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17

摘要： The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least 75% sequence identity to the amino acid sequence set out in SEQ ID NO: 2 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.

摘要翻译： 本发明涉及包含编码木糖异构酶的核苷酸序列的细胞，其中所述木糖异构酶的氨基酸序列与SEQ ID NO：2所示的氨基酸序列具有至少75％的序列同一性，并且其中所述核苷酸序列 与宿主异源。 本发明的细胞可用于生产发酵产物如乙醇的方法中。 这样的方法可以包括用本发明的细胞发酵含有木糖来源的培养基，使得细胞向发酵产物发酵木糖。