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    Production of a transgenic avian by cytoplasmic injection
    4.
    发明授权
    Production of a transgenic avian by cytoplasmic injection 有权
    通过细胞质注射生产转基因禽类

    公开(公告)号：US07312374B2

    公开(公告)日：2007-12-25

    申请号：US10251364

    申请日：2002-09-18

    申请人： Jeffrey C. Rapp ,  Leandro Christmann

    发明人： Jeffrey C. Rapp ,  Leandro Christmann

    IPC分类号： C12N15/00 ,  A01K67/027

    CPC分类号： A01K67/0275 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/00 ,  C07K2317/20 ,  C07K2317/23 ,  C12N15/8509 ,  C12N2799/027 ,  C12N2800/30 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/46 ,  C12N2830/90 ,  C12N2840/203

    摘要： This invention provides methods for the stable introduction of heterologous coding sequences into the genome of a bird and expressing the coding sequences to produce desired proteins or to alter the phenotype of the bird. The present invention provides preferred methods for introducing a transgene into the cytoplasm of avian embryonic cells by cytoplasmic microinjection. The embryo then develops into a transgenic adult capable of expressing a heterologous protein and/or capable of generating a line of transgenic birds through breeding. Synthetic vectors and gene promoters useful in the methods are also provided by the present invention, as are transgenic birds that express heterologous protein and avian eggs containing heterologous protein.

    摘要翻译： 本发明提供了将异源编码序列稳定引入鸟的基因组并表达编码序列以产生所需蛋白质或改变鸟的表型的方法。 本发明提供了通过细胞质显微注射将转基因导入禽胚胎细胞的细胞质的优选方法。 然后胚胎发育成能够表达异源蛋白质和/或能够通过育种产生一系列转基因鸟类的转基因成体。 本发明还提供了可用于该方法的合成载体和基因启动子，以及表达异源蛋白质和含有异源蛋白质的禽卵的转基因鸟类。

    Production of a transgenic avian by cytoplasmic injection
    9.
    发明授权
    Production of a transgenic avian by cytoplasmic injection 有权
    通过细胞质注射生产转基因禽类

    公开(公告)号：US07550650B2

    公开(公告)日：2009-06-23

    申请号：US10679034

    申请日：2003-10-02

    申请人： Jeffrey C. Rapp ,  Leandro Christmann

    发明人： Jeffrey C. Rapp ,  Leandro Christmann

    IPC分类号： C12N15/00 ,  A01K67/027

    CPC分类号： C07K16/00 ,  A01K67/0275 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2227/30 ,  A01K2267/01 ,  C07K2317/20 ,  C07K2317/23 ,  C12N15/8509 ,  C12N2799/027 ,  C12N2800/30 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/46 ,  C12N2830/90 ,  C12N2840/203

    摘要： This invention provides methods for the stable introduction of heterologous coding sequences into the genome of a bird and expressing the coding sequences to produce desired proteins or to alter the phenotype of the bird. The present invention provides preferred methods for introducing a transgene into the cytoplasm of avian embryonic cells by cytoplasmic microinjection. The embryo then develops into a transgenic adult capable of expressing a heterologous protein and/or capable of generating a line of transgenic birds through breeding. Synthetic vectors and gene promoters useful in the methods are also provided by the present invention, as are transgenic birds that express heterologous protein and avian eggs containing heterologous protein.

    摘要翻译： 本发明提供了将异源编码序列稳定引入鸟的基因组并表达编码序列以产生所需蛋白质或改变鸟的表型的方法。 本发明提供了通过细胞质显微注射将转基因导入禽胚胎细胞的细胞质的优选方法。 然后胚胎发育成能够表达异源蛋白质和/或能够通过育种产生一系列转基因鸟类的转基因成体。 本发明还提供了可用于该方法的合成载体和基因启动子，以及表达异源蛋白质和含有异源蛋白质的禽卵的转基因鸟类。