公开(公告)号：US20050214761A1

公开(公告)日：2005-09-29

申请号：US10502083

申请日：2003-01-13

申请人： Jens Lerchl ,  Ernst Heinz ,  Thorsten Zank

发明人： Jens Lerchl ,  Ernst Heinz ,  Thorsten Zank

IPC分类号： A01H5/00 ,  A23D9/00 ,  A23K1/16 ,  A61K8/30 ,  A61K8/36 ,  A61K31/201 ,  A61K31/202 ,  A61P9/00 ,  A61P29/00 ,  C12N9/00 ,  C12N9/10 ,  C12N15/09 ,  C12N15/82 ,  C12P7/64 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/74

CPC分类号： C12P7/6427 ,  C12N9/1029 ,  C12N15/8247 ,  C12P7/6472

摘要： The invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or organisms comprising an elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives or analogs. Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.

摘要翻译： 本发明涉及具有序列SEQ ID NO：1或其同源物，衍生物或类似物的新型延伸酶基因，涉及包含该基因或其同系物，衍生物和类似物的基因构建体及其用途。 本发明还涉及包含具有序列SEQ ID NO：1的延伸酶基因或其同源物，衍生物或类似物的载体或生物体。 此外，本发明涉及一种制备多不饱和脂肪酸的方法，以及将DNA引入生产大量油，特别是具有高含量不饱和脂肪酸的油的生物体中的方法。 此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和脂肪酸的油和/或脂肪酸制剂和/或具有至少两个双键的具有较高含量的多不饱和脂肪酸的三酰基甘油制剂。

公开(公告)号：US07544859B2

公开(公告)日：2009-06-09

申请号：US10182634

申请日：2001-02-08

申请人： Ernst Heinz ,  Thorsten Zank ,  Ulrich Zähringer ,  Jens Lerchl ,  Andreas Renz

发明人： Ernst Heinz ,  Thorsten Zank ,  Ulrich Zähringer ,  Jens Lerchl ,  Andreas Renz

IPC分类号： A01H5/00 ,  C12N15/82 ,  C07H21/04

CPC分类号： C12N9/1029 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention relates to novel elongase genes with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and/or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs. Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.

摘要翻译： 本发明涉及具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7所示序列的新型延伸酶基因或其同源物，衍生物或类似物，其包含该基因构建体 基因或其同系物，衍生物和类似物及其用途。 本发明还涉及包含具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7的延伸酶基因或其同源物，衍生物和类似物的载体或转基因生物。 本发明还涉及单独使用延伸酶基因序列或与其它延伸酶和/或其它脂肪酸生物合成基因组合的用途。 本发明涉及具有序列SEQ ID NO：1或其同系物，衍生物和类似物的新型延伸酶基因。 此外，本发明涉及一种制备多不饱和脂肪酸的方法，以及将DNA引入生产大量油，特别是具有高含量不饱和脂肪酸的油的生物体中的方法。 此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和脂肪酸的油和/或脂肪酸制剂和/或具有至少两个双键的具有较高含量的多不饱和脂肪酸的三酰基甘油制剂。

公开(公告)号：US08933300B2

公开(公告)日：2015-01-13

申请号：US11682269

申请日：2007-03-05

申请人： Ernst Heinz ,  Thorsten Zank ,  Ulrich Zähringer ,  Jens Lerchl ,  Andreas Renz

发明人： Ernst Heinz ,  Thorsten Zank ,  Ulrich Zähringer ,  Jens Lerchl ,  Andreas Renz

IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N1/20 ,  C07H21/04 ,  C12N9/10 ,  C12P7/64

CPC分类号： C12N9/1029 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention relates to novel elongase genes with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and/or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs. Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.

摘要翻译： 本发明涉及具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7的序列的新型延伸酶基因或其同源物，衍生物或类似物，其包含该基因构建体 基因或其同系物，衍生物和类似物及其用途。 本发明还涉及包含具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7的延伸酶基因或其同源物，衍生物和类似物的载体或转基因生物。 本发明还涉及单独使用延伸酶基因序列或与其它延伸酶和/或其它脂肪酸生物合成基因组合的用途。 本发明涉及具有序列SEQ ID NO：1或其同系物，衍生物和类似物的新型延伸酶基因。 此外，本发明涉及一种制备多不饱和脂肪酸的方法，以及将DNA引入生产大量油，特别是具有高含量不饱和脂肪酸的油的生物体中的方法。 此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和脂肪酸的油和/或脂肪酸制剂和/或具有至少两个双键的具有较高含量的多不饱和脂肪酸的三酰基甘油制剂。

公开(公告)号：US20080160054A1

公开(公告)日：2008-07-03

申请号：US11682269

申请日：2007-03-05

申请人： Ernst Heinz ,  Thorsten Zank ,  Ulrich Zahringer ,  Jens Lerchl ,  Andreas Renz

发明人： Ernst Heinz ,  Thorsten Zank ,  Ulrich Zahringer ,  Jens Lerchl ,  Andreas Renz

IPC分类号： A61K8/02 ,  C07H21/04 ,  C07K16/00 ,  C12N15/00 ,  C12N5/00 ,  A23D7/00 ,  C12N5/02 ,  C12P21/04 ,  G01N33/53 ,  A61K36/00

CPC分类号： C12N9/1029 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention relates to a novel elongase gene with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and/or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs.Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.

摘要翻译： 本发明涉及具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7所示序列的新型延伸酶基因或其同源物，衍生物或类似物，其包含 该基因或其同系物，衍生物和类似物，以及其用途。 本发明还涉及包含具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7的延伸酶基因或其同源物，衍生物和类似物的载体或转基因生物。 本发明还涉及单独使用延伸酶基因序列或与其它延伸酶和/或其它脂肪酸生物合成基因组合的用途。 本发明涉及具有序列SEQ ID NO：1或其同系物，衍生物和类似物的新型延伸酶基因。 此外，本发明涉及一种制备多不饱和脂肪酸的方法，以及将DNA引入生产大量油，特别是具有高含量不饱和脂肪酸的油的生物体中的方法。 此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和脂肪酸的油和/或脂肪酸制剂和/或具有至少两个双键的具有较高含量的多不饱和脂肪酸的三酰基甘油制剂。

公开(公告)号：US07179647B2

公开(公告)日：2007-02-20

申请号：US10502083

申请日：2003-01-13

申请人： Jens Lerchl ,  Ernst Heinz ,  Thorsten Zank

发明人： Jens Lerchl ,  Ernst Heinz ,  Thorsten Zank

IPC分类号： C12N5/00 ,  C12N9/02 ,  C12N9/88 ,  C12P7/64 ,  C07H21/04 ,  C12N15/00 ,  C12N1/20

CPC分类号： C12P7/6427 ,  C12N9/1029 ,  C12N15/8247 ,  C12P7/6472

摘要： The invention relates to a novel elongase gene with the sequence SEQ ID NO: 1 or its homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or organisms comprising an elongase gene with the sequence SEQ ID No: 1 or its homologs, derivatives or analogs. Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacyiglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.

摘要翻译： 本发明涉及具有序列SEQ ID NO：1或其同源物，衍生物或类似物的新型延伸酶基因，涉及包含该基因或其同系物，衍生物和类似物的基因构建体及其用途。 本发明还涉及包含具有序列SEQ ID No：1的延伸酶基因或其同源物，衍生物或类似物的载体或生物体。 此外，本发明涉及一种制备多不饱和脂肪酸的方法，以及将DNA引入生产大量油，特别是具有高含量不饱和脂肪酸的油的生物体中的方法。 此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和脂肪酸的油和/或脂肪酸制剂和/或具有较高含量的具有至少两个双键的多不饱和脂肪酸的三酰甘油制剂。

公开(公告)号：US07537920B2

公开(公告)日：2009-05-26

申请号：US10547447

申请日：2004-01-29

申请人： Andreas Renz ,  Ernst Heinz ,  Amine Abbadi ,  Frederic Domergue ,  Thorsten Zank

发明人： Andreas Renz ,  Ernst Heinz ,  Amine Abbadi ,  Frederic Domergue ,  Thorsten Zank

IPC分类号： C12N9/12 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N15/8247 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6472

摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.

摘要翻译： 本发明涉及通过将核酸引入生物体中来生产多不饱和脂肪酸的方法，该生物体编码具有酰基-CoA：溶血磷脂一羧酰基转移酶活性的多肽。 有利地，如果合适的话，这些核酸序列可以与编码脂肪酸或脂质代谢的生物合成多肽的其它核酸序列一起在转基因生物中表达。 本发明还涉及核酸序列，包含本发明的核酸序列的核酸构建体，包含核酸序列和/或核酸构建体的载体以及包含上述核酸序列的转基因生物，核酸 构建体和/或载体。 本发明的另一部分涉及通过本发明的方法生产的油，脂和/或脂肪酸及其用途。

公开(公告)号：US08373024B2

公开(公告)日：2013-02-12

申请号：US12768227

申请日：2010-04-27

申请人： Petra Cirpus ,  Jörg Bauer ,  Thorsten Zank ,  Ernst Heinz

发明人： Petra Cirpus ,  Jörg Bauer ,  Thorsten Zank ,  Ernst Heinz

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N1/20 ,  C07H21/04

CPC分类号： C12N9/0071 ,  C12N9/0004 ,  C12N15/8247

摘要： The present invention relates to nucleic acid sequences coding for polypeptides with ω-3-desaturase activity. The invention furthermore relates to nucleic acid constructs, vectors and organisms comprising at least one nucleic acid sequence according to the invention, at least one vector comprising the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms comprise the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.

摘要翻译： 本发明涉及编码具有ω-3-去饱和酶活性的多肽的核酸序列。 本发明还涉及包含至少一个本发明核酸序列的至少一种核酸构建体，载体和生物体，至少一种包含核酸序列和/或核酸构建体的载体，转基因生物体包含上述核酸序列 ，核酸构建体和/或载体。

公开(公告)号：US20080155705A1

公开(公告)日：2008-06-26

申请号：US10566944

申请日：2004-07-16

申请人： Thorsten Zank ,  Jorg Bauer ,  Petra Cirpus ,  Amine Abbadi ,  Ernst Heinz ,  Xiao Qiu ,  Patricia Vrinten ,  Petra Sperling ,  Frederic Domergue ,  Astrid Meyer ,  Jelena Kirsch

发明人： Thorsten Zank ,  Jorg Bauer ,  Petra Cirpus ,  Amine Abbadi ,  Ernst Heinz ,  Xiao Qiu ,  Patricia Vrinten ,  Petra Sperling ,  Frederic Domergue ,  Astrid Meyer ,  Jelena Kirsch

IPC分类号： A01K67/00 ,  C12N15/82 ,  C07C51/00 ,  C12N15/11 ,  A01H5/00 ,  C12N15/00 ,  C12N1/20

CPC分类号： C12N15/8247 ,  A23D9/00 ,  A23D9/02 ,  A23L33/115 ,  A23V2002/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/0093 ,  C12N9/1029 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472 ,  C12Y114/19 ,  C12Y114/19003 ,  C12Y203/01199 ,  Y02P20/52

摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceae such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.

公开(公告)号：US08486671B2

公开(公告)日：2013-07-16

申请号：US12417171

申请日：2009-04-02

申请人： Andreas Renz ,  Ernst Heinz ,  Amine Abbadi ,  Frederic Domergue ,  Thorsten Zank

发明人： Andreas Renz ,  Ernst Heinz ,  Amine Abbadi ,  Frederic Domergue ,  Thorsten Zank

IPC分类号： C12P7/64 ,  C12N9/12

CPC分类号： C12N15/8247 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6472

摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.

公开(公告)号：US08729337B2

公开(公告)日：2014-05-20

申请号：US13342142

申请日：2012-01-02

申请人： Jens Lerchl ,  Andreas Renz ,  Ernst Heinz ,  Frederic Domergue ,  Ulrich Zähringer

发明人： Jens Lerchl ,  Andreas Renz ,  Ernst Heinz ,  Frederic Domergue ,  Ulrich Zähringer

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N1/20 ,  C07H21/04

CPC分类号： C12N9/0083 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

摘要： The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention furthermore relates to the use of nucleic acid sequences SEQ ID NO: 1, 3, 5, 9, and 11 encoding polypeptides having desaturase or elongase activity in the method and for generating a transgenic organism, preferably a transgenic plant or a transgenic microorganism, with an increased content of fatty acids, oils or lipids with unsaturated C18-, C20-, or C22-fatty acids, and to their homologs or derivatives, to gene constructs encompassing these genes, and to their use alone or in combination with biosynthesis genes of polyunsaturated fatty acids. The invention also relates to multiexpression cassettes for seed-specific expression, and to vectors or organisms which encompass a desaturase gene alone or in combination with further desaturases and/or elongase genes or homologs using said expression cassettes.

摘要翻译： 本发明涉及生产具有至少两个双键的不饱和脂肪酸的方法。 本发明还涉及在该方法中编码具有去饱和酶或延长酶活性的多肽的核酸序列SEQ ID NO：1,3,5,9和11的用途，并用于产生转基因生物，优选转基因植物或转基因微生物 ，具有不饱和C18-，C20-或C22-脂肪酸的脂肪酸，油脂或脂质以及它们的同系物或衍生物，包括这些基因的基因构建物的含量增加，以及它们单独使用或与生物合成相结合 多不饱和脂肪酸的基因。 本发明还涉及用于种子特异性表达的多表达盒以及包含去饱和酶基因单独或与进一步去饱和酶和/或延伸酶基因或使用所述表达盒的同源物组合的载体或生物体。