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公开(公告)号：US4978624A
公开(公告)日：1990-12-18
申请号：US244185
申请日：1988-09-14
CPC分类号： G01N33/5094 , G01N1/30 , G01N2001/305 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： This invention relates to a method for the rapid determination of a differential white blood cell count in a sample, which method comprises:(a) preparing a reagent solution comprisingformaldehyde or paraformaldehyde;a surfactant;a sugar or sugar alcohol; anda buffer; and(b) rapidly mixing the reagent solution with the sample to be analyzed to form a reaction mixture, wherein both the reagent solution and the sample are initially at room temperature; and(c) rapidly heating the reaction mixture to a temperature of from about 62.degree. C. to about 72.degree. C. in order to lyse the red blood cells in the sample and fix the white blood cells in said sample. The invention also relates to the reagent solution of part (a) of the method.
摘要翻译： 本发明涉及一种用于快速测定样品中差异白细胞计数的方法，该方法包括：（a）制备包含甲醛或多聚甲醛的试剂溶液; 表面活性剂; 糖或糖酒; 和缓冲区; 和（b）将试剂溶液与要分析的样品快速混合以形成反应混合物，其中试剂溶液和样品都开始在室温下; 和（c）将反应混合物快速加热至约62℃至约72℃的温度，以溶解样品中的红细胞并固定所述样品中的白细胞。 本发明还涉及该方法的（a）部分的试剂溶液。
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公开(公告)号：US4801549A
公开(公告)日：1989-01-31
申请号：US9423
申请日：1987-02-02
CPC分类号： G01N33/5094 , G01N1/30 , G01N2001/305 , Y10T436/101666 , Y10T436/107497
摘要： This invention relates to a method for the rapid determination of a differential white blood cell count in a sample, which method comprises:(a) preparing a reagent solution comprising;formaldehyde or paraformaldehyde;a surfactant;a sugar or sugar alcohol; anda buffer; and(b) rapidly mixing the reagent solution with the sample to be analyzed to form a reaction mixture, wherein both the reagent solution and the sample are initially at room temperature; and(c) rapidly heating the reaction mixture to a temperature of from about 62.degree. C. to about 72.degree. C. in order to lyse the red blood cells in the sample and fix the white blood cells in the sample.
摘要翻译： 本发明涉及一种用于快速测定样品中差异白细胞计数的方法，该方法包括：（a）制备试剂溶液，其包含： 甲醛或多聚甲醛; 表面活性剂; 糖或糖酒; 和缓冲区; 和（b）将试剂溶液与要分析的样品快速混合以形成反应混合物，其中试剂溶液和样品都开始在室温下; 和（c）将反应混合物快速加热至约62℃至约72℃的温度，以溶解样品中的红细胞并固定样品中的白细胞。
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公开(公告)号：US5516695A
公开(公告)日：1996-05-14
申请号：US297662
申请日：1994-08-29
申请人： Young R. Kim , Johanna Kantor , James E. Gill , Sue E. Luptovic
发明人： Young R. Kim , Johanna Kantor , James E. Gill , Sue E. Luptovic
IPC分类号： G01N33/48 , A01N1/02 , A61K49/00 , C12N1/06 , C12Q1/02 , G01N1/28 , G01N1/30 , G01N15/14 , G01N33/49 , G01N33/50 , G01N33/569 , G01N33/80
CPC分类号： C12N1/06 , G01N15/1459 , G01N33/5094 , G01N33/56972 , G01N33/80 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10S436/805 , Y10S436/808 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25
摘要： A multipurpose reagent system for rapid analysis of a whole blood sample allowing the determination of at least five classes of peripheral white blood cells, nucleated red blood cells, and lymphocyte immunophenotyping on automated hematology instrumentation. The multipurpose reagent system lyses red cells rapidly, while it concurrently fixes white cells and preserves surface antigens on lymphocytes. The multipurpose reagent system comprises from about 3 to 7 grams per liter of a non-quaternary ammonium salt, from about 0.04 to about 0.10 percent by volume of an aliphatic aldehyde with one to four carbons, from about 10 mM to about 20 mM of a non-phosphate buffer which is inert to the aliphatic aldehyde, and a sufficient amount of water to give a pH between 5.5 and 7.5 and an osmolality of between about 160 to about 310 mOsm per liter.
摘要翻译： 用于快速分析全血样品的多用途试剂系统，允许在自动血液仪器上测定至少五类外周白细胞，有核红细胞和淋巴细胞免疫表型分析。 多用途试剂系统迅速溶解红细胞，同时固定白细胞并保留淋巴细胞上的表面抗原。 多用途试剂系统包含约3至7克/升非季铵盐，约0.04至约0.10体积％的具有一至四个碳的脂族醛，约10mM至约20mM的 对脂族醛是惰性的非磷酸盐缓冲剂，以及足够量的水以产生5.5至7.5之间的pH和约160至约310mOsm /升的渗透压。
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公开(公告)号：US5631165A
公开(公告)日：1997-05-20
申请号：US488532
申请日：1995-06-07
CPC分类号： B01F5/0453 , B01F5/046 , G01N15/14 , G01N15/1404 , G01N35/0092 , G01N35/1004 , G01N35/109 , G01N1/14 , G01N2015/0076 , G01N2015/0084 , G01N2015/1413 , G01N2035/00356 , G01N2035/00524 , G01N2035/1032 , G01N35/10 , Y10S436/805 , Y10T436/11 , Y10T436/119163 , Y10T436/25 , Y10T436/25125 , Y10T436/2575
摘要： A device for analyzing a whole blood sample is provided. The device comprises a conventional hematology analyzer integrated with a fluorescence cytometry analyzer. A controller is provided for controlling the analyzers, obtaining and utilizing data from both and reporting a quantitative result. Methods are also provided for analyzing a whole blood sample. One such method comprises the steps of performing on a single instrument an analysis of impedance associated with the blood sample, an analysis of light scatter associated with the blood sample, and an analysis of fluorescence associated with the blood sample. Data is collected and utilized. A result is reported.
摘要翻译： 提供了用于分析全血样品的装置。 该装置包括与荧光细胞分析仪集成的常规血液分析仪。 提供控制器用于控制分析器，获得和利用来自两者的数据并报告定量结果。 还提供了用于分析全血样品的方法。 一种这样的方法包括以下步骤：在单个仪器上执行与血液样品相关的阻抗的分析，与血液样品相关联的光散射的分析以及与血液样品相关联的荧光的分析。 收集和利用数据。 结果报告。
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公开(公告)号：US5122453A
公开(公告)日：1992-06-16
申请号：US188803
申请日：1988-05-03
IPC分类号： G01N33/569
CPC分类号： G01N33/56972 , Y10S436/805 , Y10S436/825
摘要： A flow cytometry method for reproducibly detecting and counting a lymphocyte population of interest in a leukocyte suspension or whole blood sample in which the red cells are subsequently lysed. The suspension (or sample) is combined with a reagent comprising a primary antibody, either native, carrying an attached enzyme or biotin or other label, and a fixative reagent, in either order. Where the enzyme is not attached, an enzyme is coupled specifically to the primary antibody. The fixed suspension is reacted with a color-producing enzyme-cytochemical reagent. The suspension, now including stained and unstained fixed cells, is passed through a flow cytometer and the cells are characterized and counted on the basis of their light-scattering and light-absorbing properties.
摘要翻译： 一种用于可重复地检测和计数白细胞悬浮液或全血样品中感兴趣的淋巴细胞群的流式细胞术方法，其中红细胞随后被裂解。 将悬浮液（或样品）与包含天然的，携带附着的酶或生物素或其它标记的一级抗体和固定剂的试剂以任何顺序组合。 当酶不连接时，酶与第一抗体特异性地偶联。 将固定的悬浮液与产生色素的酶 - 细胞化学试剂反应。 现在包括染色和未染色的固定细胞的悬浮液通过流式细胞仪，并且基于它们的光散射和光吸收性质对细胞进行表征和计数。
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公开(公告)号：US5647651A
公开(公告)日：1997-07-15
申请号：US430336
申请日：1995-04-28
申请人： Young R. Kim
发明人： Young R. Kim
摘要： A lockable storage compartment can be mounted in and removed from a medicine cabinet housing in a manner similar to that in which the shelves are mounted and removed. The storage compartment includes a fixed panel and a movable panel. The movable panel is connected to the fixed panel by one or more hinges at the forward edge of the fixed panel and can be swung to open or close the storage compartment. The storage compartment may have a catch that is releasable only from inside the storage compartment for preventing removal of the storage compartment from the medicine cabinet when the storage compartment is closed and locked.
摘要翻译： 可以以与安装和移除搁架的方式相似的方式将可锁定的储藏室安装在药盒外壳中并从药盒柜中取出。 储物箱包括固定面板和可移动面板。 可移动面板通过一个或多个铰链在固定面板的前边缘处连接到固定面板，并且可以摆动以打开或关闭储藏室。 储藏室可以具有只能从储藏室内部释放的卡子，用于当储存室关闭和锁定时，用于防止从药盒取出储藏室。
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公开(公告)号：US4412004A
公开(公告)日：1983-10-25
申请号：US277539
申请日：1981-06-26
申请人： Leonard Ornstein , Young R. Kim
发明人： Leonard Ornstein , Young R. Kim
CPC分类号： G01N33/96 , G01N2496/05 , Y10T436/101666 , Y10T436/106664
摘要： A method is disclosed of blood serum sample preparation for improved, more accurate and precise, electro-optical method for measuring erythrocyte volumes, individually and as an average.
摘要翻译： 公开了一种血清样品制备方法，用于单独和平均测量红细胞体积的改进，更准确和精确的电光方法。
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公开(公告)号：US5612223A
公开(公告)日：1997-03-18
申请号：US524128
申请日：1995-08-31
申请人： Young R. Kim , Stephen D. Stroupe
发明人： Young R. Kim , Stephen D. Stroupe
CPC分类号： G01N33/5094 , G01N33/721 , Y10T436/10 , Y10T436/107497 , Y10T436/25375
摘要： A cyanide-free method and reagent for determining the concentration of total hemoglobin in a whole blood sample accurately in less than 10 seconds comprising a ligand selected from the group consisting of imidiazole, imidazole derivatives, N-hydroxyacetamide, N-hydroxyl amine, pyridine, oxazole, thiazole, pyrazole, pyrimidine, purine, quinoline, and isoquinoline, and a surfactant with strong erythrolytic capability selected from the group consisting of lauryl dimethylamine oxide and octylphenoxy polyethoxyethanol. The reagent pH is adjusted to about 11 to about 14. Rapid mixing of the reagent with a blood sample leads to the formation of a stable chromogen whose absorbance can be measured between 540 and 550 nm. The cyanide-free reagent is ideal for use on an automated high through-put clinical hematology analyzer.
摘要翻译： 一种不含氰化物的方法和用于在小于10秒钟内精确测定全血样品中总血红蛋白浓度的试剂，其包含选自亚氨基二唑，咪唑衍生物，N-羟基乙酰胺，N-羟基胺，吡啶， 恶唑，噻唑，吡唑，嘧啶，嘌呤，喹啉和异喹啉，以及选自月桂基二甲基氧化胺和辛基苯氧基聚乙氧基乙醇的具有强的红细胞能力的表面活性剂。 将试剂pH调节至约11至约14.试剂与血液样品的快速混合导致形成稳定的色原，其吸光度可以在540和550nm之间测量。 无氰试剂非常适用于自动化高通量临床血液分析仪。
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公开(公告)号：US5589394A
公开(公告)日：1996-12-31
申请号：US356412
申请日：1994-12-15
申请人： Young R. Kim , Kenneth E. Iles , Roderick W. Larue
发明人： Young R. Kim , Kenneth E. Iles , Roderick W. Larue
CPC分类号： B01F5/0453 , B01F5/046 , G01N15/14 , G01N15/1404 , G01N35/0092 , G01N35/1004 , G01N35/109 , G01N1/14 , G01N2015/0076 , G01N2015/0084 , G01N2015/1413 , G01N2035/00356 , G01N2035/00524 , G01N2035/1032 , G01N35/10 , Y10T436/11 , Y10T436/119163 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： Embodiments described herein provide apparatuses and methods for preparing fluid. In one method, a heater thermally associated with a housing for receiving fluid is operated to apply thermal energy to the housing. A second predetermined volume of a second fluid is introduced to the housing. The second predetermined volume of the second fluid is moved with the housing for a first predetermined time period. The first predetermined volume of the first fluid is introduced to the housing. The first predetermined volume of first fluid and the second predetermined volume of second fluid are moved with the housing for a second predetermined time period. The first predetermined volume of first fluid and the second predetermined volume of second fluid are removed from the housing. Another embodiment provides an apparatus comprising a first housing for containing fluid. A second housing is operatively connected with the first housing. A prime mover is fixedly-attached to the second housing and is operatively connected with the first housing such that the first housing moves responsive to the prime mover and such that the second housing remains substantially stationary with respect to the prime mover. A fluid inlet is fluidly connected with the first housing for supplying fluid to the first housing. A fluid outlet is fluidly connected with the first housing for removing fluid from the first housing.
摘要翻译： 本文所述的实施例提供了用于制备流体的装置和方法。 在一种方法中，与用于接收流体的壳体热相关联的加热器被操作以将热能施加到壳体。 第二流体的第二预定体积被引入壳体。 第二流体的第二预定体积与壳体一起移动第一预定时间段。 将第一流体的第一预定体积引入壳体。 第一预定体积的第一流体和第二预定体积的第二流体与壳体一起移动第二预定时间段。 将第一预定体积的第一流体和第二预定体积的第二流体从壳体移除。 另一个实施例提供一种包括用于容纳流体的第一壳体的装置。 第二壳体与第一壳体可操作地连接。 原动机固定地附接到第二壳体并且与第一壳体可操作地连接，使得第一壳体响应于原动机而移动，并且使得第二壳体相对于原动机基本上保持静止。 流体入口与第一壳体流体连接以将流体供应到第一壳体。 流体出口与第一壳体流体连接以从第一壳体移除流体。
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公开(公告)号：US5484196A
公开(公告)日：1996-01-16
申请号：US179066
申请日：1994-01-10
申请人： Young R. Kim
发明人： Young R. Kim
摘要： Shelves that have a length less than the full width of the interior of a medicine cabinet housing may be relocated in housing to accommodate articles of different sizes. The housing has horizontal slots along its side walls and vertical slots on its rear wall. Each shelf fits between a side wall and a vertical slot. One end of each shelf has a tab for engaging the horizontal slots, and the other end has a hook for engaging the vertical slots. Two shelves may be installed in a side-by-side configuration to provide a surface that has a length approximately equal to the full width of the interior of the housing by engaging their hooks in the same vertical slot.
摘要翻译： 长度小于药箱外壳内部宽度的货架可以重新定位在外壳中以容纳不同尺寸的物品。 壳体沿其侧壁具有水平槽，在其后壁上具有垂直槽。 每个搁板均配合在侧壁和垂直槽之间。 每个搁架的一端具有用于接合水平狭槽的突片，另一端具有用于接合垂直槽的钩。 两个搁架可以以并排构造安装，以通过将其钩钩在相同的垂直槽中来提供具有近似等于壳体内部全宽的长度的表面。
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