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1.发明授权Immunizing compositions comprising Vibrio cholerae expressing heterologous antigens 失效包含表达异源抗原的霍乱弧菌的免疫组合物
公开(公告)号：US5747028A
公开(公告)日：1998-05-05
申请号：US480510
申请日：1995-06-07
IPC分类号： C12N15/09 , A61K39/00 , C07H21/04 , C07K14/245 , C07K14/25 , C07K14/28 , C12N1/21 , C12N15/74 , C12P21/02 , C12R1/63 , A61K39/106
CPC分类号： A61K39/107 , C07K14/245 , C07K14/25 , C07K14/28 , C12N15/74 , A61K2039/523 , A61K39/00
摘要： A bacterial cell (preferably a gram-negative, enteric bacterium such as V. cholerae) the chromosome of which which contains a DNA sequence encoding a heterologous antigen, which sequence is functionally linked to an iron-regulated promoter such as the irgA promoter of V. cholerae.
摘要翻译： 细菌细胞（优选革兰氏阴性，肠道细菌如霍乱弧菌）其染色体含有编码异源抗原的DNA序列，该序列与铁调节的启动子功能性连接，例如V的irgA启动子 霍乱。
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2.发明授权公开(公告)号：US08642042B2
公开(公告)日：2014-02-04
申请号：US11890565
申请日：2007-08-07
申请人： John J. Mekalanos
发明人： John J. Mekalanos
CPC分类号： A61K39/095 , A61K39/02 , A61K39/0275 , A61K39/0283 , A61K39/07 , A61K39/092 , A61K39/385 , A61K47/42 , A61K2039/6037 , A61K2039/645 , Y02A50/472 , Y02A50/482 , Y02A50/484
摘要： The invention relates to vaccine compositions having a carrier protein and an antigen of interest entrapped in a complex, methods of making such vaccines, and methods of vaccine administration.
摘要翻译： 本发明涉及具有载体蛋白和包含在复合物中的目的抗原的疫苗组合物，制备这种疫苗的方法和疫苗施用方法。
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公开(公告)号：US5674736A
公开(公告)日：1997-10-07
申请号：US486719
申请日：1995-06-07
IPC分类号： A61K39/00 , A61K39/02 , A61K39/112 , A61P31/04 , C07K14/255 , C12N1/21 , C12N15/01 , C12N15/09 , C12N15/74 , C12Q1/68 , C12R1/01 , C12R1/42 , C12N1/20 , C12N1/36
CPC分类号： C12R1/42 , A61K39/0275 , C07K14/255 , C12N15/01 , C12N15/74 , A61K2039/522 , A61K39/00
摘要： A vector capable of integrating into the chromosome of Salmonella including a first DNA sequence encoding a heterologous protein, a second DNA sequence encoding a marker, and a third DNA sequence encoding a phoP regulatory region regulated gene product necessary for virulence, the third DNA sequence being mutationally inactivated
摘要翻译： 能够整合入沙门氏菌染色体的载体，包括编码异源蛋白质的第一DNA序列，编码标志物的第二DNA序列和编码毒力所必需的phoP调节区域调节基因产物的第三DNA序列，第三DNA序列为 突变失活
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公开(公告)号：US5434065A
公开(公告)日：1995-07-18
申请号：US58299
申请日：1993-05-06
CPC分类号： C12N15/1082 , C07K14/255 , C12N15/52 , C12N15/62 , C12N15/907 , C12Q1/6897 , C07K2319/00 , C07K2319/10 , C07K2319/80
摘要： A genetic system termed in vivo expression technology was devised that positively selects for microbial genes that are specifically induced when microbes infect their host. The method of this invention comprises complementing the growth of an auxotrophic or antibiotic sensitive microorganism by integrating an expression vector by way of homologous recombination into the auxotrophic or antibiotic sensitive microorganism's chromosome and inducing the expression of a synthetic operon which encodes transcripts, the expression of which are easily monitored both in vitro and in vivo.
摘要翻译： 设计了一种称为体内表达技术的遗传系统，可以积极选择当微生物感染宿主时特异性诱导的微生物基因。 本发明的方法包括通过将表达载体通过同源重组整合到营养缺陷型或抗生素敏感性微生物的染色体中并诱导编码转录物的合成操纵子的表达来补充营养缺陷型或抗生素敏感性微生物的生长，其表达 在体外和体内均易于监测。
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5.发明授权Systematic identification of essential genes by in vitro transposon mutagenesis 失效通过体外转座子诱变系统鉴定必需基因公开(公告)号：US06207384B1
公开(公告)日：2001-03-27
申请号：US09277565
申请日：1999-03-26
IPC分类号： C12Q102
CPC分类号： C12N15/1082 , C12N15/102 , C12N15/1034 , C12Q1/6811 , Y02A50/52
摘要： The invention features a general system for the identification of essential genes in organisms. This system is applicable to the discovery of novel target genes for antimicrobial compounds, as well as to the discovery of genes that enhance cell growth or viability.
摘要翻译： 本发明具有用于鉴定生物体中必需基因的一般系统。 该系统适用于发现抗微生物化合物的新型靶基因，以及发现增强细胞生长或活力的基因。
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公开(公告)号：US5945285A
公开(公告)日：1999-08-31
申请号：US882455
申请日：1997-06-26
摘要： The invention provides bacterial strains with increased sensitivity to antibiotics and methods for making and using the strains.
摘要翻译： 本发明提供对抗生素敏感性增加的细菌菌株以及制备和使用菌株的方法。
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公开(公告)号：US5674724A
公开(公告)日：1997-10-07
申请号：US486715
申请日：1995-06-07
IPC分类号： A61K39/00 , A61K39/02 , A61K39/112 , A61P31/04 , C07K14/255 , C12N1/21 , C12N15/01 , C12N15/09 , C12N15/74 , C12Q1/68 , C12R1/01 , C12R1/42 , C12N1/36 , C12N1/20 , C12P21/02
CPC分类号： C12R1/42 , A61K39/0275 , C07K14/255 , C12N15/01 , C12N15/74 , A61K2039/522 , A61K39/00
摘要： A vector capable of integrating into the chromosome of Salmonella including a first DNA sequence encoding a heterologous protein, a second DNA sequence encoding a marker, and a third DNA sequence encoding a phoP regulatory region regulated gene product necessary for virulence, the third DNA sequence being mutationally inactivated.
摘要翻译： 能够整合入沙门氏菌染色体的载体，包括编码异源蛋白质的第一DNA序列，编码标志物的第二DNA序列和编码毒力所必需的phoP调节区域调节基因产物的第三DNA序列，第三DNA序列为 突变失活
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8.发明授权Genetically stable cholera vaccines with deletions of ctxA, recA and attRS1 失效具有ctxA，recA和attRS1缺失的遗传稳定的霍乱疫苗
公开(公告)号：US5631010A
公开(公告)日：1997-05-20
申请号：US403584
申请日：1995-03-14
申请人： John J. Mekalanos
发明人： John J. Mekalanos
IPC分类号： C12N15/09 , A61K39/106 , A61P31/00 , A61P31/04 , A61P37/04 , C07K14/28 , C07K14/72 , C12N1/20 , C12N1/21 , C12N15/01 , C12R1/63
CPC分类号： A61K39/107 , C07K14/28 , C07K14/72 , C12N15/01 , C12R1/63 , A61K2039/522 , A61K2039/542 , C07K2319/00 , Y10S435/909
摘要： The invention features a nontoxigenic genetically stable mutant strain of V. cholerae which lacks any functional attRS1 sequences is useful as a live, oral vaccine for inducing immunological protection against cholera and a method for making same. The invention also features a killed, oral cholera vaccine comprising at least a first and a second V. cholerae strain, wherein at least one of the strains is a different serotype, and the vaccine also contains cholera toxin B subunit, produced by at least one of the serotypes.
摘要翻译： 本发明的特征在于一种无毒基因遗传稳定的霍乱弧菌突变菌株，其缺乏任何功能性attRS1序列可用作用于诱导针对霍乱的免疫保护的活的口服疫苗及其制备方法。 本发明还具有包含至少第一和第二霍乱弧菌菌株的杀死的口服霍乱疫苗，其中至少一种菌株是不同的血清型，并且疫苗还含有由至少一种产生的霍乱毒素B亚基 的血清型。
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公开(公告)号：US4882278A
公开(公告)日：1989-11-21
申请号：US489958
申请日：1983-04-29
申请人： John J. Mekalanos
发明人： John J. Mekalanos
CPC分类号： A61K39/107 , C07K14/28 , C12N15/01 , A61K2039/522 , Y10S435/909
摘要： A genetically stable nontoxinogenic form of the Ogawa 395 strain of Vibrio cholerae which has a deletion mutation in both copies of the A.sub.1 -peptide-encoding gene, resulting in the loss of a gene sequence required for expression of a toxic A.sub.1 peptide is disclosed, as well as plasmids and methods for making the strain. The strain is useful as a live or dead oral vaccine.
摘要翻译： 公开了一种在A1-肽编码基因的两个拷贝中具有缺失突变的霍乱弧菌Ogawa 395菌株的遗传稳定的无毒素形式，导致表达有毒的A1肽所需的基因序列的丧失，作为 以及用于制备菌株的质粒和方法。 该菌株可用作活的或死的口服疫苗。
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10.发明授权Attenuated auxotrophic microorganisms having a combination of non-attenuating mutations and method for making same 失效具有非减毒突变组合的减毒营养缺陷型微生物及其制备方法公开(公告)号：US06254874B1
公开(公告)日：2001-07-03
申请号：US08421207
申请日：1995-04-13
申请人： John J. Mekalanos , Karl E. Klose
发明人： John J. Mekalanos , Karl E. Klose
IPC分类号： A61K3900
CPC分类号： C12N9/0016 , A61K2039/521 , C07K14/255 , C12R1/42
摘要： A live non-virulent vaccine composition and method for preparing the same comprising a virulent microorganismal strain which contains at least two mutations, wherein the first mutation results in an auxotrophic mutant which requires for proliferation, a nutrient which is normally available in the host tissues in an amount required by the auxotrophic mutant for proliferation and the second mutation results in the inability of the auxotrophic mutant to specifically transport the required nutrient from host-tissues into the auxotrophic mutant thereby producing an attenuated strain.
摘要翻译： 一种活的非毒性疫苗组合物及其制备方法，其包含含有至少两个突变的有毒微生物菌株，其中第一突变导致需要增殖的营养缺陷型突变体，通常在宿主组织中可获得的营养物 营养缺陷突变体对增殖所需的量和第二突变导致营养缺陷型突变体不能将所需的营养物质从宿主组织转运到营养缺陷突变体中，从而产生减毒菌株。
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