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    Sugarcane bacilliform viral (SCBV) enhancer and its use in plant functional genomics
    1.
    发明授权
    Sugarcane bacilliform viral (SCBV) enhancer and its use in plant functional genomics 有权
    甘蔗杆菌病毒（SCBV）增强子及其在植物功能基因组学中的应用

    公开(公告)号：US08785612B2

    公开(公告)日：2014-07-22

    申请号：US13220564

    申请日：2011-08-29

    申请人： John P. Davies ,  Vaka S. Reddy ,  William M. Ainley ,  Mark A. Thompson

    发明人： John P. Davies ,  Vaka S. Reddy ,  William M. Ainley ,  Mark A. Thompson

    IPC分类号： C12N15/00 ,  C12N15/09 ,  C12N15/113 ,  C12N5/04 ,  C12N15/82

    CPC分类号： C12Q1/701 ,  A01H5/10 ,  C12N15/8205 ,  C12N15/8216 ,  C12N15/8261 ,  C12Q2600/13 ,  Y02A40/146

    摘要： Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

    摘要翻译： 鉴定新的增强子序列在植物功能基因组学中具有显着的效用。 已经鉴定出甘蔗杆状坏死病毒（SCBV）转录增强子。 该增强子可用于增加基因启动子的转录速率和激活标记实验。 当将4×阵列的SCBV增强子置于玉米醇脱氢酶最小启动子的截短形式的上游时，观察到转录增加10倍。 描述了使用SCBV转录增强子的方法，嵌合转录调控区，构建体，细胞，组织和包含增强子一个或多个拷贝的生物体也是如此。

    Activation tagging platform for maize, and resultant tagged populations and plants
    4.
    发明授权
    Activation tagging platform for maize, and resultant tagged populations and plants 有权
    用于玉米的激活标记平台，以及得到的标签种群和植物

    公开(公告)号：US08912393B2

    公开(公告)日：2014-12-16

    申请号：US13220570

    申请日：2011-08-29

    申请人： John P. Davies ,  Vaka S. Reddy ,  William M. Ainley ,  Xing Liang Liu ,  D. Ry Wagner

    发明人： John P. Davies ,  Vaka S. Reddy ,  William M. Ainley ,  Xing Liang Liu ,  D. Ry Wagner

    IPC分类号： C12N15/82 ,  C12N5/00 ,  C12N5/02 ,  C12N15/00 ,  A01H5/00

    CPC分类号： C12N15/8209 ,  C12N15/8205 ,  C12N15/8241 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/16

    摘要： Disclosed herein is an activation tagging construct for maize, resulting tagged populations and plants. In one example, an activation tagging DNA construct includes a coding sequence for a transposase, a detectable reporter (such as anthocyanin regulatory genes B-Peru and C1) and a non-autonomous transposable T-DNA cassette. For example, the transposable T-DNA cassette is inserted into the detectable reporter encoding region such that the B-Peru and C1 genes express anthocyanins in a cell containing the maize activation tagging DNA construct only upon excision of the transposable cassette. Methods of generating a tagged population of maize plants include transforming a maize plant cell or tissue with the disclosed constructs.

    摘要翻译： 本文公开了一种用于玉米的激活标签构建体，得到标签种群和植物。 在一个实例中，激活标记DNA构建体包括转座酶的编码序列，可检测的报道分子（如花青素调节基因B-Peru和C1）和非自主转座T-DNA盒。 例如，将可转座T-DNA盒插入可检测的报道编码区，使得B-Peru和C1基因仅在切换转座盒时才在含有玉米激活标记DNA构建体的细胞中表达花青素。 产生标记的玉米植物群的方法包括用所公开的构建体转化玉米植物细胞或组织。

    RESISTANCE TO AUXINIC HERBICIDES
    5.
    发明申请
    RESISTANCE TO AUXINIC HERBICIDES 有权

    公开(公告)号：US20120064539A1

    公开(公告)日：2012-03-15

    申请号：US13304189

    申请日：2011-11-23

    申请人： Terence A. Walsh ,  Glenn Hicks ,  Mary Honma ,  John P. Davies

    发明人： Terence A. Walsh ,  Glenn Hicks ,  Mary Honma ,  John P. Davies

    IPC分类号： G01N33/53

    CPC分类号： C12N15/8274 ,  C07K14/415

    摘要： The invention provides methods of identifying herbicidal auxins. The invention further provides auxin-herbicide-resistant plants and genes conferring auxin-herbicide resistance. This invention also provides a method of identifying other proteins that bind picolinate auxins from additional plant species. The invention further provides a method to identify the molecular binding site for picolinate auxins. The invention also includes the use of the picolinate herbicidal auxin target site proteins, and methods of discovering new compounds with herbicidal or plant growth regulatory activity. The invention also includes methods for producing plants that are resistant to picolinate herbicidal auxins. Specific examples of novel proteins associated with herbicide binding include AFB5, AFB4, and SGT1b.