公开(公告)号：US20120142035A1

公开(公告)日：2012-06-07

申请号：US13401263

申请日：2012-02-21

申请人： John W. Backus ,  Jian Zheng ,  George Bashirians

发明人： John W. Backus ,  Jian Zheng ,  George Bashirians

IPC分类号： G01N33/53

CPC分类号： G01N33/96 ,  G01N2496/80 ,  Y10T436/105831

摘要： Disclosed herein are compositions and methods comprising two or more proteins in which at least one of the proteins has been altered to reduce their mutual recognition and binding. Such compositions are useful as reference, calibrators or controls in methods and assays for determining the amount of one or more of the proteins that may be present in a sample of interest or in confirming the presence of one or more of the proteins in the sample. More particularly, it relates to compositions and methods comprising altered placental growth factor-1 (PlGF-1) and soluble fms-like tyrosine kinase (sFlt-1) and methods for determining the amount or confirming the presence of sFlt-1 and/or PlGF-1 in a sample of interest.

摘要翻译： 本文公开了包含两种或更多种蛋白质的组合物和方法，其中至少一种蛋白质已被改变以减少它们的相互识别和结合。 这样的组合物可用作用于测定可能存在于感兴趣样品中的一种或多种蛋白质的量的方法和测定中的参考，校准物或对照，或确认样品中一种或多种蛋白质的存在。 更具体地说，本发明涉及包含改变的胎盘生长因子-1（PlGF-1）和可溶性fms样酪氨酸激酶（sFlt-1）的组合物和方法以及用于确定量或确认sFlt-1和/或 PlGF-1在感兴趣的样本中。

公开(公告)号：US08148157B2

公开(公告)日：2012-04-03

申请号：US12349695

申请日：2009-01-07

申请人： John W. Backus ,  Jian Zheng ,  George Bashirians

发明人： John W. Backus ,  Jian Zheng ,  George Bashirians

IPC分类号： G01N31/00

CPC分类号： G01N33/96 ,  G01N2496/80 ,  Y10T436/105831

摘要： Disclosed herein are compositions and methods comprising two or more proteins in which at least one of the proteins has been altered to reduce their mutual recognition and binding. Such compositions are useful as reference, calibrators or controls in methods and assays for determining the amount of one or more of the proteins that may be present in a sample of interest or in confirming the presence of one or more of the proteins in the sample. More particularly, it relates to compositions and methods comprising altered placental growth factor-1 (PlGF-1) and soluble fms-like tyrosine kinase (sFlt-1) and methods for determining the amount or confirming the presence of sFlt-1 and/or PlGF-1 in a sample of interest.

摘要翻译： 本文公开了包含两种或更多种蛋白质的组合物和方法，其中至少一种蛋白质已被改变以减少它们的相互识别和结合。 这样的组合物可用作用于测定可能存在于感兴趣样品中的一种或多种蛋白质的量的方法和测定中的参考，校准物或对照，或确认样品中一种或多种蛋白质的存在。 更具体地说，本发明涉及包含改变的胎盘生长因子-1（PlGF-1）和可溶性fms样酪氨酸激酶（sFlt-1）的组合物和方法以及用于确定量或确认sFlt-1和/或 PlGF-1在感兴趣的样本中。

公开(公告)号：US08940492B2

公开(公告)日：2015-01-27

申请号：US13401263

申请日：2012-02-21

申请人： John W. Backus ,  Jian Zheng ,  George Bashirians

发明人： John W. Backus ,  Jian Zheng ,  George Bashirians

IPC分类号： G01N33/53 ,  G01N33/96

CPC分类号： G01N33/96 ,  G01N2496/80 ,  Y10T436/105831

摘要： Disclosed herein are compositions and methods comprising two or more proteins in which at least one of the proteins has been altered to reduce their mutual recognition and binding. Such compositions are useful as reference, calibrators or controls in methods and assays for determining the amount of one or more of the proteins that may be present in a sample of interest or in confirming the presence of one or more of the proteins in the sample. More particularly, it relates to compositions and methods comprising altered placental growth factor-1 (PlGF-1) and soluble fms-like tyrosine kinase (sFlt-1) and methods for determining the amount or confirming the presence of sFlt-1 and/or PlGF-1 in a sample of interest.

摘要翻译： 本文公开了包含两种或更多种蛋白质的组合物和方法，其中至少一种蛋白质已被改变以减少它们的相互识别和结合。 这样的组合物可用作参考，校准物或对照，用于确定可能存在于感兴趣样品中的一种或多种蛋白质的量或用于确认样品中一种或多种蛋白质的存在的方法和测定中。 更具体地说，本发明涉及包含改变的胎盘生长因子-1（PlGF-1）和可溶性fms样酪氨酸激酶（sFlt-1）的组合物和方法以及用于确定量或确认sFlt-1和/或 PlGF-1在感兴趣的样本中。

公开(公告)号：US20090176264A1

公开(公告)日：2009-07-09

申请号：US12349695

申请日：2009-01-07

申请人： John W. Backus ,  Jian Zheng ,  George Bashirians

发明人： John W. Backus ,  Jian Zheng ,  George Bashirians

IPC分类号： C12Q1/48

CPC分类号： G01N33/96 ,  G01N2496/80 ,  Y10T436/105831

摘要： Disclosed herein are compositions and methods comprising two or more proteins in which at least one of the proteins has been altered to reduce their mutual recognition and binding. Such compositions are useful as reference, calibrators or controls in methods and assays for determining the amount of one or more of the proteins that may be present in a sample of interest or in confirming the presence of one or more of the proteins in the sample. More particularly, it relates to compositions and methods comprising altered placental growth factor-1 (PlGF-1) and soluble fms-like tyrosine kinase (sFlt-1) and methods for determining the amount or confirming the presence of sFlt-1 and/or PlGF-1 in a sample of interest.

摘要翻译： 本文公开了包含两种或更多种蛋白质的组合物和方法，其中至少一种蛋白质已被改变以减少它们的相互识别和结合。 这样的组合物可用作用于测定可能存在于感兴趣样品中的一种或多种蛋白质的量的方法和测定中的参考，校准物或对照，或确认样品中一种或多种蛋白质的存在。 更具体地说，本发明涉及包含改变的胎盘生长因子-1（PlGF-1）和可溶性fms样酪氨酸激酶（sFlt-1）的组合物和方法以及用于确定量或确认sFlt-1和/或 PlGF-1在感兴趣的样本中。

公开(公告)号：US6126839A

公开(公告)日：2000-10-03

申请号：US13987

申请日：1998-01-27

申请人： Carol Kreader ,  John W. Backus ,  Joanne H. Kerschner ,  Rashmi Mehta

发明人： Carol Kreader ,  John W. Backus ,  Joanne H. Kerschner ,  Rashmi Mehta

IPC分类号： G01N33/48 ,  C12N1/00 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/32 ,  G01N33/50 ,  B01D21/26 ,  B01D21/01

CPC分类号： C12R1/32 ,  C12N1/005

摘要： The present invention relates to methods for concentrating bacteria from a viscous biological sample. The methods involve adding to the sample a water-soluble, density-lowering agent having a density of 0.7 to 0.9 g/ml and a boiling point greater than 50.degree. C. The invention also relates to methods for concentrating bacteria and free bacterial nucleic acids from a biological sample that involve mixing with the sample a density-lowering agent and a monovalent salt.

摘要翻译： 本发明涉及浓缩来自粘性生物样品的细菌的方法。 该方法包括向样品中加入密度为0.7至0.9g / ml且沸点大于50℃的水溶性密度降低剂。本发明还涉及浓缩细菌和游离细菌核酸的方法 从涉及与样品混合的生物样品中降低密度的剂和单价盐。

公开(公告)号：US5582988A

公开(公告)日：1996-12-10

申请号：US306870

申请日：1994-09-15

申请人： John W. Backus ,  Tobias E. Ekeze ,  Jerome C. Swartz ,  Richard C. Sutton ,  Ignazio S. Ponticello ,  JoAnne H. Kerschner ,  John B. Findlay

发明人： John W. Backus ,  Tobias E. Ekeze ,  Jerome C. Swartz ,  Richard C. Sutton ,  Ignazio S. Ponticello ,  JoAnne H. Kerschner ,  John B. Findlay

IPC分类号： C12N15/09 ,  C07H21/04 ,  C07K14/045 ,  C07K14/16 ,  C07K14/35 ,  C08F20/34 ,  C08F20/60 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/543 ,  G01N33/569

CPC分类号： C12N15/1003 ,  C07K14/005 ,  C07K14/35 ,  C08F20/34 ,  C08F20/60 ,  C12N15/1006 ,  C12Q1/6806 ,  C12N2710/16122 ,  C12N2740/16022

摘要： Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with specific weakly basic polymers to form a precipitate with the nucleic acids at acidic pH. After removing non-precipitated materials, the pH is then made basic, thereby releasing the nucleic acids from the polymer. This method for preparing specimen samples is simple and quite rapid, and the released nucleic acids can be further treated in hybridization assays or amplification procedures. The weakly basic polymers are water-soluble and cationic at acidic pH, but neutral in charge at basic pH.

摘要翻译： 通过使溶解产物与特定的弱碱性聚合物接触以在酸性pH下与核酸形成沉淀物，使核酸可用于扩增或裂解后的其它处理。 除去非沉淀物后，将pH调至碱性，从而将聚合物中的核酸释放出来。 制备标本样品的这种方法是简单且相当快速的，并且释放的核酸可以在杂交测定或扩增程序中进一步处理。 弱碱性聚合物在酸性pH下为水溶性和阳离子型，而在碱性pH下为负电荷。

公开(公告)号：US20110275079A1

公开(公告)日：2011-11-10

申请号：US12742920

申请日：2008-11-13

申请人： John F. Palma ,  John W. Backus ,  Yixin Wang ,  Jack X. Yu ,  Yi Zhang ,  Tatiana Vener ,  Carlo Derecho ,  Dong U. Lee

发明人： John F. Palma ,  John W. Backus ,  Yixin Wang ,  Jack X. Yu ,  Yi Zhang ,  Tatiana Vener ,  Carlo Derecho ,  Dong U. Lee

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/6883 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/53 ,  G01N33/6893 ,  G16B25/00 ,  G16B40/00 ,  G16H50/20 ,  Y02A90/26

摘要： Methods are disclosed for the identification of gene sets that are differentially expressed in PBMCs of patients diagnosed with a pre-diabetic disease state and overt type II diabetes. 3 gene and 10 gene signatures are shown to accurately predict a diabetic disease state in a patient. The application also described kits for the rapid diagnosis of diabetic disease states in patients at a point of care facility.

摘要翻译： 公开了用于鉴定在诊断患有糖尿病前状态和明显II型糖尿病的患者的PBMC中差异表达的基因组的方法。 3基因和10个基因特征被证明准确预测患者的糖尿病状态。 该应用还描述了在护理设施点的患者中快速诊断糖尿病疾病状态的试剂盒。

公开(公告)号：US6001558A

公开(公告)日：1999-12-14

申请号：US102830

申请日：1998-06-23

申请人： John W. Backus ,  Susan M. Atwood ,  Ann E. Casey ,  Eric B. Rasmussen ,  Thomas J. Cummins

发明人： John W. Backus ,  Susan M. Atwood ,  Ann E. Casey ,  Eric B. Rasmussen ,  Thomas J. Cummins

IPC分类号： G01N33/566 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/569

CPC分类号： C12Q1/703

摘要： The present invention relates to methods and test kits for the amplification and detection of nucleic acids from human immunodeficiency virus (HIV) type 1 and/or type 2. The methods use multiple primer sets to amplify all subtypes of HIV-1, including group M and group O isolates, and all subtypes of HIV-2.

摘要翻译： 本发明涉及用于扩增和检测来自1型和/或2型人类免疫缺陷病毒（HIV）的核酸的方法和测试试剂盒。该方法使用多个引物组来扩增HIV-1的所有亚型，包括M组 和O组分离株，以及HIV-2的所有亚型。

公开(公告)号：US5674717A

公开(公告)日：1997-10-07

申请号：US548078

申请日：1995-10-25

申请人： John W. Backus ,  William Harold Donish ,  John Bruce Findlay ,  John William H. Sutherland ,  Marlene M. King

发明人： John W. Backus ,  William Harold Donish ,  John Bruce Findlay ,  John William H. Sutherland ,  Marlene M. King

IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12Q1/70 ,  C12P19/34

CPC分类号： C12Q1/6851 ,  C12Q1/6848 ,  C12Q1/686

摘要： Nucleic acids can be amplified and detected using a very rapid polymerase chain reaction procedure in which two different nucleic acid sequences are present. This method allows one to preferentially modulate (for example, suppress) the degree of amplification of one or more nucleic acid sequences relative to other nucleic acid sequences. This modulation is achieved by exploiting differences in the relative primer melt temperatures, or by using certain ratios of primers. Each PCR cycle is very fast, that is less than about 90 seconds. This method is particularly useful for amplification and detection of DNA associated with infectious agents that may be present in a specimen in very small quantities compared to other nontargeted nucleic acids.

摘要翻译： 可以使用非常快速的聚合酶链反应程序扩增和检测核酸，其中存在两种不同的核酸序列。 该方法允许优先调节（例如，抑制）相对于其它核酸序列的一个或多个核酸序列的扩增程度。 通过利用相对底漆熔融温度的差异，或通过使用一定比例的引物来实现该调节。 每个PCR循环非常快，即小于约90秒。 与其他非靶向核酸相比，该方法特别可用于扩增和检测与可能以非常小的量存在于样品中的感染因子相关联的DNA。

公开(公告)号：US06280930B1

公开(公告)日：2001-08-28

申请号：US08845739

申请日：1997-04-25

申请人： John W. Backus ,  Marcia L. Kramer ,  Joseph Falvo

发明人： John W. Backus ,  Marcia L. Kramer ,  Joseph Falvo

IPC分类号： C12Q168

CPC分类号： C12Q1/6844 ,  C12Q2527/101 ,  C12Q2521/101

摘要： The present invention relates to a method for amplifying and detecting a target nucleic acid. The method comprising contacting a sample suspected of containing the target nucleic acid with a thermostable DNA polymerase and two primers that are substantially complementary to the target nucleic acid, under conditions such that the target nucleic acid is amplified. The amplified target nucleic acids are then denatured to form single stranded nucleic acids. Following amplification, the sample is subject to a pre-detection incubation step. The sample is incubated for between 1 second and 30 minutes at between 95° C. and 120° C. to inactivate said polymerization agent. Finally, the presence or absence of the amplified target nucleic acids is determined. Preferably, amplification, incubation and detection are carried out in a closed reaction vessel.

摘要翻译： 本发明涉及用于扩增和检测靶核酸的方法。 该方法包括将疑似含有目标核酸的样品与热稳定DNA聚合酶和基本上与靶核酸互补的两个引物，使得靶核酸被扩增的条件下接触。 然后将扩增的靶核酸变性以形成单链核酸。 扩增后，对样品进行预检测孵育步骤。 将样品在95℃和120℃之间孵育1秒至30分钟以使所述聚合剂失活。 最后，测定扩增的靶核酸的存在或不存在。 优选地，扩增，孵育和检测在封闭的反应容器中进行。