Diagnostic compositions, elements, methods and test kits for
amplification and detection of retroviral DNA using primers having
matched melting temperatures
    4.
    发明授权
    Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures 失效
    使用具有匹配熔融温度的引物扩增和检测逆转录病毒DNA的诊断组合物,元件,方法和测试试剂盒

    公开(公告)号:US5403707A

    公开(公告)日:1995-04-04

    申请号:US062022

    申请日:1993-05-14

    CPC分类号: C12Q1/686 C12Q1/6834

    摘要: An aqueous composition containing primers for opposing strands of a target retroviral DNA (such as HIV-I DNA) can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of that target DNA and one or more additional target DNA's. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T.sub.m within the range of from about 65.degree. to about 74.degree. C., while all of the T.sub.m 's are within about 5.degree. C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes, all of which have T.sub.m 's which are greater than 50.degree. C. and within 15.degree. C. of each other.

    摘要翻译: 可以在聚合酶链反应中使用含有针对靶向逆转录病毒DNA(例如HIV-1 DNA)的相对链的引物的含水组合物,以便同时快速且有效地扩增和检测该靶DNA和一种或多种另外的靶DNA。 每个靶DNA的引物长度不等于5个核苷酸,Tm在约65-74℃范围内,而所有Tm在彼此的约5℃内。 这样的组合物可用于诊断测试试剂盒和用于多重核酸的扩增和检测的方法,或使用多个捕获探针进行“多路复用”,所有这些探针具有大于50℃的Tm和15℃以内的Tm 彼此。

    Nucleic acid probe, test kit and diagnostic and purification methods
    6.
    发明授权
    Nucleic acid probe, test kit and diagnostic and purification methods 失效
    核酸探针,测试试剂盒和诊断和纯化方法

    公开(公告)号:US5328825A

    公开(公告)日:1994-07-12

    申请号:US847447

    申请日:1992-03-06

    摘要: An oligonucleotide is linked to a particle through a protein or carbohydrate to form a water-insoluble nucleic acid probe. The protein or carbohydrate has a pI of about 6 or less, and has been chemically modified with an acylating, alkylating or sulfonylating agent. The particle surface is substantially free of other proteins or carbohydrates. The probe is useful in various diagnostic and purification methods wherein hybridization of the oligonucleotide with a target nucleic acid is possible. In one instance, the probe can be used to capture a DNA strand which has been amplified using polymerase chain reaction techniques.

    摘要翻译: 寡核苷酸通过蛋白质或碳水化合物与颗粒连接以形成水不溶性核酸探针。 蛋白质或碳水化合物具有约6或更小的pI,并已用酰化,烷基化或磺酰化剂进行化学修饰。 颗粒表面基本上不含其他蛋白质或碳水化合物。 探针可用于各种诊断和纯化方法,其中寡核苷酸与靶核酸的杂交是可能的。 在一个实例中,探针可用于捕获已经使用聚合酶链反应技术扩增的DNA链。

    Nucleic acid test article and its use to detect a predetermined nucleic
acid
    9.
    发明授权
    Nucleic acid test article and its use to detect a predetermined nucleic acid 失效
    核酸测试制品及其用于检测预定核酸的用途

    公开(公告)号:US5514550A

    公开(公告)日:1996-05-07

    申请号:US300297

    申请日:1994-09-02

    IPC分类号: C12Q1/68 C12Q1/70 C12P19/34

    摘要: A nucleic acid test article can be used to detect a targeted nucleic acid found in a specimen. The test article includes a substrate having two opposing surfaces and a water-insoluble nucleic acid probe attached in a distinct zone of one of the surfaces. The probe is prepared from a water-insoluble particle to which is covalently attached an oligonucleotide which is complementary to the targeted nucleic acid. Substantially none of the probe is embedded within the surface of the substrate. Particularly useful test articles have a multiplicity of water-insoluble probes located in distinct zones on one of the substrate surfaces. These probes are useful for the detection of a multiplicity of targeted nucleic acids, particularly after amplification by polymerase chain reaction.

    摘要翻译: 核酸测试制品可用于检测样品中发现的靶向核酸。 测试制品包括具有两个相对表面的底物和附着在一个表面的不同区域中的水不溶性核酸探针。 该探针由水不溶性颗粒制备,其中共价连接与靶向核酸互补的寡核苷酸。 基本上没有一个探针嵌入基片表面。 特别有用的测试制品具有位于其中一个衬底表面上的不同区域中的多个水不溶性探针。 这些探针可用于检测多种靶向核酸,特别是通过聚合酶链反应扩增后。

    Nucleic acid material amplification and detection without washing
    10.
    发明授权
    Nucleic acid material amplification and detection without washing 失效
    核酸材料放大和检测无洗涤

    公开(公告)号:US5422271A

    公开(公告)日:1995-06-06

    申请号:US979569

    申请日:1992-11-20

    IPC分类号: B01L3/00 C12M1/00

    CPC分类号: B01L3/502

    摘要: A device and method are disclosed for amplifying and detecting nucleic acid material. The device and method use a label and signalling material responsive to the label to produce a detectable signal. A surprising result of the method and device is that at least one of the wash steps heretofore required has been eliminated without substantially adversely affecting the results.

    摘要翻译: 公开了用于扩增和检测核酸材料的装置和方法。 该装置和方法使用响应标签的标签和信令材料产生可检测信号。 该方法和装置的令人惊奇的结果是至少需要一个洗涤步骤已被消除,而不会对结果产生实质性的不利影响。