公开(公告)号：US06967085B1

公开(公告)日：2005-11-22

申请号：US10031835

申请日：2000-08-02

申请人： Jonathan Hughes ,  Steven Weir ,  Paul Moran

发明人： Jonathan Hughes ,  Steven Weir ,  Paul Moran

IPC分类号： G01N33/48 ,  B01D21/00 ,  B01D21/01 ,  C12N1/00 ,  C12N1/02 ,  C12Q1/02 ,  C12R1/125 ,  C12N11/02 ,  C12N11/08

CPC分类号： C12N1/02 ,  C02F1/56

摘要： A process of flocculating microbial cell material from a suspending medium which contains cell material, comprising adding to the suspending medium a first polymeric material which is cationic and has intrinsic viscosity of not more than 2 dl/g, and subsequently or simultaneously adding to the suspending medium a second polymeric material which is cationic or substantially non-ionic and has intrinsic viscosity of at least 4 dl/g, and allowing the cell material to flocculate.

摘要翻译： 一种从含有细胞材料的悬浮介质中絮凝微生物细胞材料的方法，包括向悬浮培养基中加入第一聚合物材料，其为阳离子型，特性粘度不大于2dl / g，随后或同时加入悬浮液中 介质是阳离子或基本上非离子的第二聚合物材料，其特性粘度至少为4dl / g，并允许电池材料絮凝。

公开(公告)号：US20100004390A1

公开(公告)日：2010-01-07

申请号：US12437435

申请日：2009-05-07

申请人： William G. Turnell ,  Zaza D. Gomurashvill ,  Benjamin W. Parcher ,  Jonathan Hughes ,  Jeffrey N. Anderl

发明人： William G. Turnell ,  Zaza D. Gomurashvill ,  Benjamin W. Parcher ,  Jonathan Hughes ,  Jeffrey N. Anderl

IPC分类号： A61K47/48 ,  C08G69/44 ,  C08L77/12

CPC分类号： C08F283/04 ,  A61K47/593 ,  A61K47/595 ,  A61K47/60 ,  A61K47/6935 ,  A61K49/0047 ,  A61K49/0093 ,  A61K49/128 ,  C08G69/44 ,  C08G73/028 ,  C08L77/12 ,  C08L79/02

摘要： The invention provides metal-chelating poly(ether amide) polymers useful in preparation of polymer compositions for delivering a variety of cargo molecules, such as bioactive agents. In solution metal ions and cargo molecules, such as vaccine epitopes, that include metal avid amino acids can be loaded into the polymer compositions and held in a non-covalent complex. Nanoparticles of such polymer compositions can also be prepared directly from the solution.

摘要翻译： 本发明提供了可用于制备用于递送各种货物分子的生物活性剂的聚合物组合物的金属螯合聚（醚酰胺）聚合物。 在溶液中，金属离子和货物分子，如疫苗表位，包括金属氨基酸，可加载到聚合物组合物中并保持在非共价复合物中。 这种聚合物组合物的纳米颗粒也可以直接从溶液制备。

公开(公告)号：US07582215B2

公开(公告)日：2009-09-01

申请号：US10523301

申请日：2003-07-30

申请人： Jonathan Hughes ,  Kenneth Charles Symes

发明人： Jonathan Hughes ,  Kenneth Charles Symes

IPC分类号： B01D37/02

CPC分类号： C02F1/56 ,  C02F1/5236 ,  C02F11/121 ,  C02F11/14 ,  C02F2101/30 ,  C02F2103/28 ,  Y02E50/17

摘要： A process of separating suspended solids from a fermentation liquor by subjecting the liquor to a solids-liquid separation stage, wherein the fermentation liquor is produced in a fermentation process for the production of a fermentation product, which liquor comprises water, lignin and BOD, wherein the solids liquid separation stage is assisted by a treatment system, characterised in that the treatment system comprises either, (i) a cationic polymer having an intrinsic viscosity (IV) of at least 4 dl/g at a dose of above 2 kg/tonne based on dry weight of suspension, or (ii) a cationic polymer having an intrinsic viscosity (IV) of at least 4 dl/g and, (iii) an anionic polymer, and/or (iv) a cationic polymer of intrinsic viscosity of below 4 dl/g and a cationic charge density of at least 3 meq/g and/or (v) inorganic coagulants and/or (vi) charged microparticulate material.

摘要翻译： 一种通过对液体进行固体 - 液体分离阶段来分离悬浮固体的方法，其中发酵液体在用于生产发酵产物的发酵方法中产生，该液体包含水，木质素和BOD，其中 固体液体分离阶段由处理系统辅助，其特征在于处理系统包括：（i）特征粘度（IV）至少为4dl / g的阳离子聚合物，剂量高于2kg / tonne 基于干悬浮液的重量，或（ii）特性粘度（IV）至少为4dl / g的阳离子聚合物，和（iii）阴离子聚合物和/或（iv）特性粘度的阳离子聚合物 低于4dl / g和至少3meq / g的阳离子电荷密度和/或（v）无机凝结剂和/或（vi）带电微粒材料。

公开(公告)号：US07575912B2

公开(公告)日：2009-08-18

申请号：US10580448

申请日：2004-11-22

申请人： Jonathan Hughes ,  Yvonne Armitage ,  Jatinder Kullar ,  Stuart Greenhalgh

发明人： Jonathan Hughes ,  Yvonne Armitage ,  Jatinder Kullar ,  Stuart Greenhalgh

IPC分类号： C12N1/20 ,  C12N1/00

CPC分类号： C12R1/01 ,  C12N1/04 ,  C12N1/20 ,  C12N9/88 ,  C12P13/02 ,  Y10S435/822

摘要： A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrite hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrite wherein the nitrite is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NUMB 41164, a mutant thereof and a nitrite hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NUMB 41164.

摘要翻译： 作为玫瑰色红球菌菌株NCIMB 41164或其突变体的微生物。 要求保护在包含尿素或尿素衍生物的培养基中培养微生物的方法。 要求从微生物获得的亚硝酸盐水合酶。 还要求的是从相应的亚硝酸盐制备酰胺的方法，其中亚硝酸盐在水性介质中在生物催化剂存在下进行水合反应，所述生物催化剂选自由玫瑰色红玫瑰色菌株NUMB 41164， 其突变体和可从Rhodococcus rhodochrous strain NCIMB 41164获得的亚硝酸盐水合酶或其突变体。 还要求保存红玫瑰色红球菌NUMB 41164的方法。

公开(公告)号：US20070238156A1

公开(公告)日：2007-10-11

申请号：US10591775

申请日：2005-03-09

申请人： Jonathan Hughes ,  Kenneth Symes

发明人： Jonathan Hughes ,  Kenneth Symes

IPC分类号： C12P13/00 ,  A01H5/00 ,  A01K67/033 ,  C12N5/04 ,  C12N5/06

CPC分类号： C12P13/00 ,  C12P7/62 ,  C12P13/001

摘要： A biocatalytic method for the synthesis of 2-(N,N-dimethylamino)ethanol and/or choline esters of acrylic acid and/or methacrylic acid, namely a method or process for the manufacture of acrylylcholine and/or methacrylylcholine, comprising reacting 2-(N,N-dimethylamino)ethanol and/or choline with acrylyl-CoA and/or methacrylyl-CoA in the presence of a biocatalyst with choline acetyltransferase activity, where preferably the acrylyl-CoA and/or methacrylyl-CoA is formed by reaction of acrylate and/or methacrylate with coenzyme A in the presence of an energy providing substance and a biocatalyst with S-acetyl coenzyme A synthetase activity (acetyl CoA synthetase activity); to (especially transformed, that is genetically modified) organisms having choline acetyltransferase activity and preferably in addition the acetyl CoA synthetase activity and their use in said process or method; the use of a biocatalyst with choline acetyltransferase activity to carry out the transfer of the (meth)acrylyl moiety from (meth)acrylyl CoA to 2-(N,N-dimethylamino)ethanol and/or choline, in order to manufacture 2-(N,N-dimethylamino)ethyl(meth)acrylate and/or (meth)acrylylcholine; and further uses, organisms, processes and methods as described in the specification.

摘要翻译： 用于合成丙烯酸和/或甲基丙烯酸的2-（N，N-二甲基氨基）乙醇和/或胆碱酯的生物催化方法，即制备丙烯酰胆碱和/或甲基丙烯酰胆碱的方法或方法，包括使2- （N，N-二甲基氨基）乙醇和/或胆碱与丙烯酰辅酶A和/或甲基丙烯酰辅酶A在具有胆碱乙酰转移酶活性的生物催化剂存在下进行，其中优选通过以下方式形成丙烯酰辅酶A和/或甲基丙烯酰辅酶A. 丙烯酸酯和/或甲基丙烯酸酯与能量提供物质和具有S-乙酰辅酶A合成酶活性的生物催化剂（乙酰辅酶A合成酶活性）存在下的辅酶A; 具有胆碱乙酰转移酶活性（特别是经转基因，即经遗传修饰的）生物体，优选地还包括乙酰CoA合成酶活性及其在所述方法或方法中的用途; 使用具有胆碱乙酰转移酶活性的生物催化剂进行（甲基）丙烯酰部分从（甲基）丙烯酰辅酶A向2-（N，N-二甲基氨基）乙醇和/或胆碱的转移，以制备2-（ N，N-二甲基氨基）乙基（甲基）丙烯酸酯和/或（甲基）丙烯酰胆碱; 以及说明书中描述的进一步的用途，生物，过程和方法。

公开(公告)号：US20070204472A1

公开(公告)日：2007-09-06

申请号：US11366048

申请日：2006-03-01

申请人： Michael Circosta ,  Conor McNamara ,  John Sinisi ,  Jonathan Hughes

发明人： Michael Circosta ,  Conor McNamara ,  John Sinisi ,  Jonathan Hughes

IPC分类号： B27B21/06

CPC分类号： B23D49/12 ,  B23D51/12 ,  B27B21/02

摘要： A hand saw including a housing defining a channel. The channel is adapted to receive a blade having a top edge, a bottom edge and two sides. The hand saw includes at least one bias member positioned adjacent to one of the sides of the blade in the housing and a lever coupled to the bias member. The lever is movable between an open position where the bias member is disengaged from the blade, and a closed position, where the bias member substantially engages at least one of the sides of the blade to secure the blade in the housing.

摘要翻译： 手锯包括限定通道的壳体。 通道适于接收具有顶部边缘，底部边缘和两侧的叶片。 手锯包括邻近壳体中的叶片的一个侧面定位的至少一个偏置构件和联接到偏置构件的杆。 杠杆可在偏压构件与叶片脱离的打开位置和关闭位置之间移动，偏压构件基本上接合叶片的至少一侧以将叶片固定在壳体中。

公开(公告)号：US20070184536A1

公开(公告)日：2007-08-09

申请号：US10580447

申请日：2004-11-22

申请人： Stuart Greenhalgh ,  Kenneth Symes ,  Yvonne Armitage ,  Jonathan Hughes ,  Gary Richardson

发明人： Stuart Greenhalgh ,  Kenneth Symes ,  Yvonne Armitage ,  Jonathan Hughes ,  Gary Richardson

IPC分类号： C12P13/02 ,  C08F220/06

CPC分类号： C12P13/02 ,  C12P7/40 ,  C12P7/46

摘要： A process for preparing a polymer of an ethylenically unsaturated monomer, in which the monomer is obtainable from a biocatalysed reaction or a fermentation process, and wherein the monomer contains cellular material and/or components of a fermentation broth, forming the polymer by polymerising the ethylenically unsaturated monomer or a monomer mixture comprising the ethylenically unsaturated monomer, wherein there is substantially no removal of the cellular material and/or components of the fermentation broth from the ethylenically unsaturated monomer.

摘要翻译： 一种制备烯键式不饱和单体的聚合物的方法，其中单体可从生物催化反应或发酵方法获得，并且其中单体含有发酵液的细胞材料和/或组分，通过聚合烯属不饱和单体形成聚合物 不饱和单体或包含烯键式不饱和单体的单体混合物，其中基本上不从烯属不饱和单体中除去发酵液的细胞材料和/或组分。

公开(公告)号：US20060111990A1

公开(公告)日：2006-05-25

申请号：US10982550

申请日：2004-11-05

申请人： Neal Cohen ,  Lewis Watson ,  Jonathan Hughes ,  Matthew Walters

发明人： Neal Cohen ,  Lewis Watson ,  Jonathan Hughes ,  Matthew Walters

IPC分类号： G07C1/10

CPC分类号： G06F17/248 ,  G06F17/211 ,  G06Q40/125

摘要： A system and method for payroll processing. In one embodiment, the system includes a first database, a second database, and a processor operably connected to the first and second databases. The first database contains information about an employer, and the second database contains a PDF template. The processor is operable to create a filable PDF tax form from the PDF template, with the filable PDF tax form including data retrieved from and/or calculated from the first database. The method of the present invention involves the steps of retrieving with the processor a PDF template from the second database and at least one data from the first database, and then producing a filable PDF tax form from the retrieved PDF template and from the retrieved and/or calculate data from the first database. The present invention provides an employer with control over the generation, validation, correction, completion, and even filing of the employer's tax forms, and creates forms viewable and printable by a variety of systems.

摘要翻译： 一种用于工资单处理的系统和方法。 在一个实施例中，系统包括可操作地连接到第一和第二数据库的第一数据库，第二数据库和处理器。 第一个数据库包含有关雇主的信息，第二个数据库包含PDF模板。 处理器可操作以从PDF模板创建可归档的PDF税表，其中可申请的PDF税表包括从第一个数据库检索和/或从第一个数据库计算的数据。 本发明的方法涉及以下步骤：用处理器检索来自第二数据库的PDF模板和来自第一数据库的至少一个数据，然后从所检索的PDF模板和从检索到的和/ 或从第一个数据库计算数据。 本发明提供了雇主对生成，验证，更正，完成甚至归档雇主的税表的控制权，并且创建了可由各种系统查看和打印的表格。

公开(公告)号：US20050272134A1

公开(公告)日：2005-12-08

申请号：US10523230

申请日：2003-07-28

申请人： Jonathan Hughes

发明人： Jonathan Hughes

IPC分类号： C13K1/02 ,  C12P7/62

CPC分类号： C12P7/10 ,  C12P2201/00 ,  C12P2203/00 ,  C13K1/02 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

摘要： A process of producing fermentation product comprising the steps of, (i) forming an acidified suspension of particulate plant derived material comprising a first polysaccharide which is more readily hydrolysable and a second polysaccharide which is more difficult to hydrolyse, (ii) allowing the first polysaccharide to undergo hydrolysis by action of the acid at a temperature of at least 50° C. under conditions such that the first polysaccharide is hydrolysed and thereby forming a mixture of an aqueous liquor containing dissolved sugar and a solid residue containing the second polysaccharide, (iii) subjecting the mixture to one or more separation stages in which the solid residue and aqueous sugar liquor are substantially separated from each other, (iv) optionally washing the residue substantially free of the acid and the sugar, (v) passing the solid cellulosic residue to a further treatment stage in which the residue is subjected to the action of dilute acid at a temperature of at least 50° C. under conditions such that the second polysaccharide is hydrolysed and thereby forming a mixture of an aqueous liquor containing dissolved sugar and a solid residue, (vi) subjecting the mixture to one or more separation stages in which the solid residue and aqueous sugar liquor are substantially separated from each other, (vii) optionally washing the residue substantially free of the sugar, (viii) adjusting the pH of the aqueous liquor from stages (iii), (iv), (vi) and (vii) to a pH of at least 4, (ix) passing the aqueous liquor from stage (viii) to a fermentation stage in order to produce a fermentation product, (x) separating the fermentation product from the broth, wherein the separation stage in steps (iii) and/or (vi) is assisted by flocculation of the waste by-product, employing one or more flocculating agent(s) which are water soluble or swellable polymers or charged microparticulate material. Typically such fermentation products include for instance ethanol, glycerol, acetone, n-butanol, butanediol, isopropanol, butyric acid, methane, citric acid, fumaric acid, lactic acid, propionic acid, succinic acid, itaconic acid, acetic acid, acetaldehyde, 3-hydroxypropionic acid, glyconic acid, tartaric acid and amino acids such as L-glutaric acid, L-lysine, L-aspartic acid, L-tryptophan, L-arylglycines or salts of any of these acids.

公开(公告)号：US06969847B2

公开(公告)日：2005-11-29

申请号：US11056530

申请日：2005-02-11

申请人： Stephen Davis ,  Alexander A. Makarov ,  Jonathan Hughes

发明人： Stephen Davis ,  Alexander A. Makarov ,  Jonathan Hughes

IPC分类号： G01N27/62 ,  G01Q30/04 ,  G01Q60/00 ,  H01J49/02 ,  H01J49/06 ,  H01J49/40 ,  H01J49/04

CPC分类号： H01J49/025

摘要： A mass spectrometer comprises an ion source which produces an ion beam from a substance to be analysed and a detector to detect a quantity of ions incident thereon. The detector includes two elements (16, 18) each of which detect a part of the quantity of ions and an attenuation device attenuates the quantity of ions reaching one of the detector elements. At least one of the detector elements (16, 18) is connected to a time to digital converter (TDC) to allow counting of the ions and at least one of the detector elements is connected in parallel to both a time to digital converter (TDC) and an analogue to digital converter (ADC).