METHOD
    5.
    发明申请
    METHOD 审中-公开
    方法

    公开(公告)号:US20140051146A1

    公开(公告)日:2014-02-20

    申请号:US14000933

    申请日:2012-02-16

    IPC分类号: C12N9/18

    摘要: In one aspect there is provided a method for producing a recombinant enzyme capable of hydrolysing chlorophyll or a chlorophyll derivative, comprising a step of intracellular expression of the recombinant enzyme in a eukaryotic host cell.

    摘要翻译: 一方面,提供了能够水解叶绿素或叶绿素衍生物的重组酶的方法,其包括在真核宿主细胞中细胞内表达重组酶的步骤。

    Transcription factors
    6.
    发明授权
    Transcription factors 有权
    转录因子

    公开(公告)号:US08017341B2

    公开(公告)日:2011-09-13

    申请号:US11738355

    申请日:2007-04-20

    摘要: The present invention relates to an isolated nucleic acid sequence coding for a PntR transcription factor comprising a nucleotide sequence that is the same as, or is complementary to, or contains any suitable codon substitutions for any of those of SEQ ID NOs: 1, 3 or 5 or comprises a sequence which has at least 60% sequence homology with any of SEQ ID NOs: 1, 3 or 5, as well as corresponding polypeptides, vector systems and host cells comprising the same. In particular, the invention relates to sequences which are obtained from an Aspergillus, Trichoderma or Penicillium cell. The invention further relates to methods for disrupting PntR expression in a cell as well as methods for expression or production of a POI in a host cell in which PntR expression has been disrupted.

    摘要翻译: 本发明涉及编码PntR转录因子的分离的核酸序列,所述核酸序列包含与SEQ ID NO:1,3或任何SEQ ID NO:1,3中任一个相同或互补的任何合适的密码子取代的核苷酸序列或 或包含与SEQ ID NO:1,3或5中任一个具有至少60%序列同源性的序列,以及相应的多肽,载体系统和包含该序列的宿主细胞。 特别地,本发明涉及从曲霉属,木霉属或青霉属细胞获得的序列。 本发明还涉及破坏细胞中PntR表达的方法以及在PntR表达被破坏的宿主细胞中表达或产生POI的方法。