-
1.发明授权Sulphocoumarin-containing nucleotides and their use in processes for detecting nucleic acids 失效含有磺基香豆素的核苷酸及其在核酸检测方法中的应用
公开(公告)号：US5547860A
公开(公告)日：1996-08-20
申请号：US185548
申请日：1994-01-21
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C09B57/02 , C09B69/10 , C09K11/06 , C12Q1/68 , G01N33/533 , G01N33/58 , G01N33/68 , C12P19/34 , C07H21/04 , C12Q1/70
CPC分类号： C07H21/00 , G01N33/533
摘要： The invention relates to the preparation, as substituents, of nucleotides possessing a fluorescent coumarin residue, the enzymic incorporation of these nucleotides into nucleic acids and the detection of nucleic acids of defined sequence by hybridization with a complementary, coumarin-labelled nucleic acid.
摘要翻译： 本发明涉及作为取代基制备具有荧光香豆素残基的核苷酸，将这些核苷酸酶引入核酸以及通过与互补的香豆素标记的核酸杂交来检测所定义序列的核酸。
-
公开(公告)号：US5298583A
公开(公告)日：1994-03-29
申请号：US875167
申请日：1992-04-28
申请人： Ludger Heiliger , Hans-Ulrich Siegmund , Herbert Hugl , Antonius Lobberding , Eberhard Kuckert , Bruno Bomer , Thomas Bocker , Gunther Franke
发明人： Ludger Heiliger , Hans-Ulrich Siegmund , Herbert Hugl , Antonius Lobberding , Eberhard Kuckert , Bruno Bomer , Thomas Bocker , Gunther Franke
IPC分类号： C09B69/10 , G01N33/533 , C08F228/02 , C08F220/06 , C08F220/10 , C08F220/56
CPC分类号： G01N33/533 , C09B69/10
摘要： The present invention relates to polymer dyestuffs, i.e. polymer-bound dyestuffs, to a process for their preparation and to their use, for example as markers in analytical procedures. Polymer dyestuffs contain linkable functional groups and are water-soluble under customary conditions of analysis. The polymer portion is usually responsible for this water solubility. The dyestuffs per se are often water-insoluble.
摘要翻译： 本发明涉及聚合物染料，即聚合物结合的染料，其制备方法及其用途，例如作为分析程序中的标记物。 聚合物染料含有可连接的官能团，在常规的分析条件下是水溶性的。 聚合物部分通常负责这种水溶性。 染料本身通常是不溶于水的。
-
公开(公告)号：US5453461A
公开(公告)日：1995-09-26
申请号：US131368
申请日：1993-10-04
IPC分类号： A61K31/415 , A61K31/70 , C08F4/00 , C08F4/04 , C08F4/28 , C08F4/32 , C08F8/00 , C08G83/00 , C08G85/00 , C08L101/00 , C08L101/02 , C09B69/10 , G01N33/53 , G01N33/543
CPC分类号： G01N33/54353 , C08F8/00
摘要： The present invention relates to novel, biologically active polymers, methods for their preparation and their use for biochemical identification reactions and/or for further reaction with biologically active molecules such as, for example, proteins or nucleic acids.
摘要翻译： 本发明涉及新的生物活性聚合物，其制备方法及其用于生物化学鉴定反应和/或与生物活性分子例如蛋白质或核酸的进一步反应的用途。
-
公开(公告)号：US20060135665A1
公开(公告)日：2006-06-22
申请号：US11301772
申请日：2005-12-13
摘要： Ester mixtures comprising a mixture of esterification products formed from polyether polyols, one or more aromatic monocarboxylic acids or aromatic monocarboxylic acid derivatives and one or more aliphatic monocarboxylic acids or aliphatic monocarboxylic acid derivatives, to a process for their preparation, and to their use as plasticizers for polymers.
摘要翻译： 酯混合物，其包含由聚醚多元醇，一种或多种芳族单羧酸或芳族单羧酸衍生物形成的酯化产物和一种或多种脂肪族单羧酸或脂族一元羧酸衍生物的混合物，其制备方法及其作为增塑剂的用途 用于聚合物。
-
5.发明授权Coumarin derivatives, process for their preparation, their use and thiazolyl acetic acid derivatives as intermediates 失效香豆素衍生物，其制备方法及其用途和噻唑乙酸衍生物作为中间体
公开(公告)号：US5300656A
公开(公告)日：1994-04-05
申请号：US915772
申请日：1992-07-16
IPC分类号： G01N33/533 , C07D277/20 , C07D277/32 , C07D277/56 , C07D311/16 , C07D417/04 , C07D417/14 , G01N21/64 , G01N33/53 , G01N33/542 , G01N33/543 , G01N33/545 , G01N33/552 , G01N33/553 , G01N33/58 , C07D405/04 , C07D11/80 , C07D405/12
CPC分类号： C07D277/32 , C07D277/56 , C07D311/16 , C07D417/04 , G01N21/6428 , G01N33/542 , G01N33/54373 , G01N33/545 , G01N33/552 , G01N33/553 , G01N33/582
摘要： Coumarin deviatives of the formula ##STR1## in which R.sup.1 denotes cyano,R.sup.2 represents phenyl or thiazolyl bonded in the 2-, 4-, or 5-position,R.sup.3 denotes hydrogen, C.sub.1 -C.sub.4 -alkyl or C.sub.1 -C.sub.4 -alkoxycarbonyl-C.sub.1 -C.sub.4 -alkyl andR.sup.4 represents hydrogen, C.sub.1 -C.sub.4 -alkyl or phenylsulphonyl, where C.sub.1 -C.sub.4 -alkyl can be substituted by hydroxyl, amino, carboxyl or C.sub.1 -C.sub.4 -alkoxy-carbonyl and phenyl can be monosubstituted or disubstituted by chlorine, bromine or C.sub.1 -C.sub.4 -alkyl, where furthermore R.sup.3 and R.sup.4, together with the N atom which they substitute, can denote a morpholine ring, a piperazine ring or a triazole ring which can carry one or two substituents from the group comprising methyl, ethyl and phenyl, andwhere furthermore one of the radicals R.sup.2, R.sup.3 and R.sup.4 denotes or carries a primary or secondary amino group, the hydroxyl group, the carboxyl group or the C.sub.1 -C.sub.4 -alkoxy-carbonyl group or can be converted into such a group by hydrolysis or hydrogenation, are suitable for dyeing biologically active compounds.
摘要翻译： 其中R 1表示氰基，R 2表示在R 2的2-，4-或5-位键合的苯基或噻唑基的式（I）的香豆素偏差表示氢，C 1 -C 4 - 烷基或C 1 -C 4 - 烷氧基羰基-C 1 -C 4 - 烷基，R 4表示氢，C 1 -C 4 - 烷基或苯基磺酰基，其中C 1 -C 4 - 烷基可以被羟基，氨基，羧基或C 1 -C 4 - 烷氧羰基取代，苯基可以是单取代或双取代 通过氯，溴或C 1 -C 4 - 烷基，此外R3和R4与它们所取代的N原子一起可以表示可以带有一个或两个取代基的吗啉环，哌嗪环或三唑环，所述取代基包括 甲基，乙基和苯基，此外，基团R 2，R 3和R 4中的一个表示或携带伯或仲氨基，羟基，羧基或C 1 -C 4 - 烷氧基 - 羰基，或可以转化为 这种通过水解或氢化的基团适用于染色生物活性化合物。
-
公开(公告)号：US20060135685A1
公开(公告)日：2006-06-22
申请号：US11301681
申请日：2005-12-13
CPC分类号： C09D5/02
摘要： The present invention relates to ester mixtures comprising trimethylolalkane esters of aromatic carboxylic acids, trimethylolalkane esters of aliphatic carboxylic acids and trimethylolalkane esters both of aromatic and aliphatic carboxylic acids, to a process for their preparation, and to their use as plasticizers for polymers.
摘要翻译： 本发明涉及包含芳族羧酸的三羟甲基烷烃酯，脂族羧酸的三羟甲基烷烃酯和芳族和脂族羧酸的三羟甲基烷烃酯的酯混合物，其制备方法，以及它们作为聚合物增塑剂的用途。
-
公开(公告)号：US5194393A
公开(公告)日：1993-03-16
申请号：US611197
申请日：1990-11-08
申请人： Herbert Hugl , Eberhard Kuckert , Dietmar Mobius , Holger Ohst , Meinhard Rolf , Hans J. Rosenkranz , Heinrich C. Schopper , Hans-Ulrich Siegmund , Klaus Sommer , Rolf Wehrmann
发明人： Herbert Hugl , Eberhard Kuckert , Dietmar Mobius , Holger Ohst , Meinhard Rolf , Hans J. Rosenkranz , Heinrich C. Schopper , Hans-Ulrich Siegmund , Klaus Sommer , Rolf Wehrmann
IPC分类号： C07D277/32 , C07D277/56 , C07D311/16 , C07D417/04 , G01N21/64 , G01N33/52 , G01N33/53 , G01N33/533 , G01N33/542 , G01N33/543 , G01N33/545 , G01N33/552 , G01N33/553 , G01N33/58
CPC分类号： C07D277/32 , C07D277/56 , C07D311/16 , C07D417/04 , G01N21/6428 , G01N33/542 , G01N33/54373 , G01N33/552 , G01N33/553 , G01N33/582 , G01N2021/6441 , Y10S435/968 , Y10S436/80 , Y10S436/805
摘要： The present invention relates to a sensor with a novel construction for a detection method of molecules labelled with fluorescent dye for detecting these dissolved substances or analytes by energy transfer with a simple fluorescence technique and increased sensitivity in the detection as well as versatile use for different tasks and the possibility of reproducible preparation of films bound to solid surfaces.
摘要翻译： 本发明涉及一种具有新颖结构的传感器，用于通过荧光染料标记的分子的检测方法，用于通过简单的荧光技术通过能量转移检测这些溶解的物质或分析物，并且在检测中增加灵敏度以及用于不同任务的通用性 以及可重复制备与固体表面结合的膜的可能性。
-
公开(公告)号：US20070049685A1
公开(公告)日：2007-03-01
申请号：US11506724
申请日：2006-08-18
IPC分类号： C08G18/08
CPC分类号： C08L75/04 , C08G2290/00 , C08K5/103
摘要： Soft, elastomeric, thermoplastic polyurethanes comprising, as plasticizer, a mixture composed of trimethylolalkane esters of aromatic carboxylic acids, of trimethylolalkane esters of aliphatic carboxylic acids, and also of trimethylolalkane esters of both aromatic and aliphatic carboxylic acids, a process for their production, and their use.
摘要翻译： 软质弹性体，热塑性聚氨酯，其包含作为增塑剂的由芳族羧酸的三羟甲基烷烃酯组成的混合物，脂族羧酸的三羟甲基烷烃酯，以及芳族和脂族羧酸的三羟甲基烷烃酯，其制备方法和 他们的使用。
-
9.发明授权Synthetic polymer particles for use as standards and calibrators in flow cytometry 失效合成聚合物颗粒，用作流式细胞仪中的标准品和校准品
公开(公告)号：US6074879A
公开(公告)日：2000-06-13
申请号：US099233
申请日：1998-06-17
申请人： David Zelmanovic , William Moskalski , Ronny DeClercq , Frank Louwet , Eberhard Kuckert , Wolfgang Podszun
发明人： David Zelmanovic , William Moskalski , Ronny DeClercq , Frank Louwet , Eberhard Kuckert , Wolfgang Podszun
CPC分类号： G01N15/1012 , G01N2015/1062 , G01N2015/1486 , G01N2015/149 , Y10T436/10 , Y10T436/101666
摘要： The invention describes novel methods for calibrating or standardizing flow cytometry instruments using synthetic polymer particles or beads having physical properties which provide advantages for their use in such instruments. The polymer particles or beads employed in the calibration methods are spherical, have an average particle diameter of from about 1 to about 8 microns, a relatively narrow particle distribution, and a low refractive index, i.e., from about 1.35 to about 1.45. The particles are safe, stable for long time periods and are particularly suitable for employment as hematology analyzer calibration substances. The invention provides an advantageous improvement over the types of standards currently used to calibrate a variety of instruments that rely on light scatter combined with other parameters to identify and measure biological particles or cells, as well as nonbiological particles. In particular, using the calibration/standardization method and polymer particles according to the present invention allows hematology instruments to be calibrated and standardized for subsequent accurate and reliable determinations of red blood cells, reticulocytes, white blood cells and platelets in whole blood samples.
摘要翻译： 本发明描述了使用具有物理性质的合成聚合物颗粒或珠粒来校准或标准化流式细胞仪器的新方法，其提供了在这些仪器中使用的优点。 在校准方法中使用的聚合物颗粒或珠粒是球形的，平均粒径为约1至约8微米，相对较窄的颗粒分布和低折射率，即约1.35至约1.45。 颗粒安全，长时间稳定，特别适合用作血液分析仪校准物质。 本发明提供了相对于目前用于校准依赖于光散射的各种仪器与其他参数相结合以识别和测量生物颗粒或细胞以及非生物颗粒的标准类型的有利改进。 特别地，使用根据本发明的校准/标准化方法和聚合物颗粒允许血液仪器被校准和标准化，用于随后准确可靠地测定全血样品中的红细胞，网织红细胞，白细胞和血小板。
-
公开(公告)号：US5409964A
公开(公告)日：1995-04-25
申请号：US946566
申请日：1992-09-18
申请人： Ludger Heiliger , Eberhard Kuckert
发明人： Ludger Heiliger , Eberhard Kuckert
IPC分类号： C08F8/00 , C08F2/46 , C08F2/50 , C09B69/10 , G01N33/533 , G01N33/58 , C08F8/30 , C08F20/08 , C08F20/56
CPC分类号： G01N33/585
摘要： Photochemically linkable polymers corresponding to formula (I)P-(A).sub.n (I)in whichP is a polymer component,A is a photochemically reactive unit andn is the number 1 or 2,and their use for immunological purposes.
摘要翻译： 对应于式（I）P-（A）n（I）的光化学可连接聚合物，其中P是聚合物组分，A是光化学反应性单元，n是数字1或2，并且它们用于免疫学目的。
-
-
-
-
-
-
-
-
-
搜索结果
16 条记录 (耗时 0.019 秒)
