High-throughput Biological Screening Assay Using Voltage Gradients
    1.
    发明申请
    High-throughput Biological Screening Assay Using Voltage Gradients 有权
    使用电压梯度的高通量生物筛选测定

    公开(公告)号:US20100176005A1

    公开(公告)日:2010-07-15

    申请号:US12683988

    申请日:2010-01-07

    IPC分类号: G01N27/26

    CPC分类号: G01N27/3271

    摘要: A high throughput biological screening assay comprising at least two anodes, at least two cathodes acting as the reference electrode, and a polymer membrane placed between each anode and cathode, wherein the at least two anodes comprise a biological culture, and wherein the at least two cathodes comprise an oxidizing agent and a buffering agent. The high throughput biological screening assay wherein the at least two cathodes are connected in parallel to simulate the connection between the same cathode and different anodes. The high throughput biological screening assay further including an external resistor or open circuit and means for measuring the voltage across the external resistor or open circuit. A method of measuring power generation using a single cathode as a reference electrode to monitor the biological production of energy. A method of correlating bacterial biofilm formation within an operational microbial fuel cell directly to current output.

    摘要翻译: 包括至少两个阳极,至少两个用作参考电极的阴极和放置在每个阳极和阴极之间的聚合物膜的高通量生物筛选测定,其中所述至少两个阳极包括生物培养物,并且其中所述至少两个阳极 阴极包括氧化剂和缓冲剂。 高通量生物筛选测定法,其中至少两个阴极并联连接以模拟相同阴极和不同阳极之间的连接。 高通量生物筛选测定还包括外部电阻器或开路以及用于测量外部电阻器或开路电压的装置。 使用单个阴极作为参考电极来测量发电的方法,以监测能量的生物产生。 将可操作的微生物燃料电池内的细菌生物膜形成与当前输出直接相关联的方法。

    Biological fuel cells with nanoporous membranes
    2.
    发明申请
    Biological fuel cells with nanoporous membranes 有权
    具有纳米多孔膜的生物燃料电池

    公开(公告)号:US20090169924A1

    公开(公告)日:2009-07-02

    申请号:US11978662

    申请日:2007-10-30

    IPC分类号: H01M8/16

    摘要: A fuel cell comprising an anode chamber, a cathode chamber, and a nanoporous membrane between the anode chamber and the cathode chamber, wherein the nanoporous membrane sequesters and isolates a microbe in the anode chamber. The nanoporous membrane allows nutrients to flow actively or passively from the cathode chamber to the anode chamber and can be modified by a thin film composite (TFC) to create a TFC nanofiltration membrane. The nanoporous membrane can have a pore size from about 100 nm to about 1000 nm. A method of making a fuel cell comprising configuring a nanoporous membrane between an anode chamber and a cathode chamber wherein the nanoporous membrane sequesters and isolates a microbe in the anode chamber and can be used to protect the cathode chamber.

    摘要翻译: 一种燃料电池,包括在阳极室和阴极室之间的阳极室,阴极室和纳米多孔膜,其中纳米多孔膜螯合并隔离阳极室中的微生物。 纳米多孔膜允许营养物质从阴极室主动或被动地流向阳极室,并且可以通过薄膜复合材料(TFC)进行改性以产生TFC纳滤膜。 纳米孔膜可具有约100nm至约1000nm的孔径。 一种制造燃料电池的方法,包括在阳极室和阴极室之间配置纳米多孔膜,其中纳米多孔膜螯合并隔离阳极室中的微生物并可用于保护阴极室。

    Biological fuel cells with nanoporous membranes
    3.
    发明授权
    Biological fuel cells with nanoporous membranes 有权
    具有纳米多孔膜的生物燃料电池

    公开(公告)号:US08048547B2

    公开(公告)日:2011-11-01

    申请号:US11978662

    申请日:2007-10-30

    IPC分类号: H01M8/16 H01M8/10 H01M4/36

    摘要: A fuel cell comprising an anode chamber, a cathode chamber, and a nanoporous membrane between the anode chamber and the cathode chamber, wherein the nanoporous membrane sequesters and isolates a microbe in the anode chamber. The nanoporous membrane allows nutrients to flow actively or passively from the cathode chamber to the anode chamber and can be modified by a thin film composite (TFC) to create a TFC nanofiltration membrane. The nanoporous membrane can have a pore size from about 100 nm to about 1000 nm. A method of making a fuel cell comprising configuring a nanoporous membrane between an anode chamber and a cathode chamber wherein the nanoporous membrane sequesters and isolates a microbe in the anode chamber and can be used to protect the cathode chamber.

    摘要翻译: 一种燃料电池,包括在阳极室和阴极室之间的阳极室,阴极室和纳米多孔膜,其中纳米多孔膜螯合并隔离阳极室中的微生物。 纳米多孔膜允许营养物质从阴极室主动或被动地流向阳极室,并且可以通过薄膜复合材料(TFC)进行改性以产生TFC纳滤膜。 纳米孔膜可具有约100nm至约1000nm的孔径。 一种制造燃料电池的方法,包括在阳极室和阴极室之间配置纳米多孔膜,其中纳米多孔膜螯合并隔离阳极室中的微生物并可用于保护阴极室。

    BOUYANCY CONTROL DEVICE
    5.
    发明申请
    BOUYANCY CONTROL DEVICE 有权
    BOUYANCY控制装置

    公开(公告)号:US20100199907A1

    公开(公告)日:2010-08-12

    申请号:US12700759

    申请日:2010-02-05

    IPC分类号: B63G8/14 F17C1/00

    CPC分类号: B63B22/18

    摘要: A device having: a chamber having a gas inlet, a gas vent, and a liquid vent; and a float and a weight coupled to the chamber. The float has a lower density than the chamber. The weight has a higher density than the chamber. The aggregate density of the chamber, the float, and the weight is greater than the density of the chamber. The gas inlet, the gas vent, the liquid vent, the float, and the weight are positioned on the chamber such that: when the chamber is filled with and submerged in a liquid in which the chamber is neutrally-buoyant, the chamber is oriented to place the gas vent below the gas inlet; and when a gas is introduced through the gas inlet into the chamber that is filled with the liquid, the chamber pivots to raise the gas vent until a portion of the gas escapes from the chamber through only the gas vent.

    摘要翻译: 一种装置,具有:具有气体入口,排气口和液体排放口的室; 以及耦合到所述室的浮子和重物。 浮子具有比室低的密度。 重量具有比室更高的密度。 室的聚集密度,浮子和重量大于室的密度。 气体入口,气体排出口,液体排出口,浮子和重物位于室上,使得:当室被填充并浸没在其中的中性浮力的液体中时,腔室被定向 将气体放置在气体入口下方; 并且当通过气体入口将气体引入到填充有液体的室中时,室枢转以升高排气口,直到气体的一部分仅通过排气口从室逸出。

    SUB-MICRON LASER DIRECT WRITE
    8.
    发明申请
    SUB-MICRON LASER DIRECT WRITE 有权
    亚微米激光直接写入

    公开(公告)号:US20080314881A1

    公开(公告)日:2008-12-25

    申请号:US12141954

    申请日:2008-06-19

    IPC分类号: B23K26/00

    摘要: A method of directing a pulse of laser energy though a workpiece. The workpiece has: a substrate that transmits the laser energy; focusing elements on a surface of the substrate proximal to the laser that focus the laser energy; and a coating on the substrate distal to the laser that absorbs a portion of the laser energy. Each focusing element focuses the laser energy to a point that removes or ablates a portion of the coating from the substrate to produce a hole in the coating.

    摘要翻译: 通过工件引导激光能量的脉冲的方法。 工件具有:传递激光能量的基板; 聚焦元件在靠近激光的基板的表面上聚焦激光能量; 以及在激光器远端的衬底上的涂层,其吸收一部分激光能量。 每个聚焦元件将激光能量聚焦到从衬底移除或消除涂层的一部分以在涂层中产生孔的点。

    Generation of viable cell active biomaterial patterns by laser transfer
    10.
    发明授权
    Generation of viable cell active biomaterial patterns by laser transfer 失效
    通过激光转移生成活细胞活性生物材料图案

    公开(公告)号:US06905738B2

    公开(公告)日:2005-06-14

    申请号:US10068315

    申请日:2002-02-08

    摘要: A method for depositing a transfer material onto a receiving substrate uses a source of laser energy, a receiving substrate, and a target substrate. The target substrate comprises a laser-transparent support having a laser-facing surface and a support surface. The target substrate also comprises a composite material having a back surface in contact with the support surface and a front surface. The composite material comprises a mixture of the transfer material to be deposited and a matrix material. The matrix material is a material that has the property that, when it is exposed to laser energy, it desorbs from the laser-transparent support. The source of laser energy is positioned in relation to the target substrate so that laser energy is directed through the laser-facing surface of the target substrate and through the laser-transparent support to strike the composite material at a defined target location. The receiving substrate is positioned in a spaced relation to the target substrate. The source of laser energy has sufficient energy to desorb the composite material at the defined target location, causing the composite material to desorb from the defined target location and be lifted from the support surface of the laser-transparent support. The composite material is deposited at a defined receiving location on the receiving substrate. The method is useful for creating a pattern of biomaterial on the receiving substrate.

    摘要翻译: 用于将转移材料沉积到接收衬底上的方法使用激光能量源,接收衬底和目标衬底。 目标基板包括具有激光面向表面和支撑表面的激光透明支撑件。 目标衬底还包括具有与支撑表面接触的后表面和前表面的复合材料。 复合材料包括待沉积的转移材料和基体材料的混合物。 基体材料是具有这样一种特性,即当暴露于激光能量时,其从激光透明支持体解吸。 激光能量源相对于目标衬底定位,使得激光能量被引导通过目标衬底的面向激光的表面并且穿过激光透明支撑件以在确定的目标位置上击打复合材料。 接收基板与目标基板间隔开定位。 激光能源具有足够的能量来在确定的目标位置解吸复合材料,导致复合材料从限定的目标位置解吸并从激光透明支撑体的支撑表面提升。 复合材料沉积在接收基底上的限定的接收位置。 该方法可用于在接收基底上产生生物材料的图案。