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    Use of dimethyl disulfide for methionine production in microoraganisms
    3.
    发明授权
    Use of dimethyl disulfide for methionine production in microoraganisms 有权
    用二甲基二硫化物在微生物激素中生产甲硫氨酸

    公开(公告)号：US08399214B2

    公开(公告)日：2013-03-19

    申请号：US11988971

    申请日：2006-07-18

    申请人： Oskar Zelder ,  Stefan Haefner ,  Andrea Herold ,  Corinna Klopprogge ,  Hartwig Schroder ,  R. Rogers Yocum ,  Mark K. Williams

    发明人： Oskar Zelder ,  Stefan Haefner ,  Andrea Herold ,  Corinna Klopprogge ,  Hartwig Schroder ,  R. Rogers Yocum ,  Mark K. Williams

    IPC分类号： C12P1/00

    CPC分类号： C12P13/12

    摘要： The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a ΔmetF organism or a ΔmetE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A ΔmetF ΔmetB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

    摘要翻译： 本发明的特征在于用于生产甲硫氨酸的改进方法和生物体。 本发明证明，一种有机蛋白（MetF）生物体或一种具有谷氨酸棒状杆菌或大肠杆菌的突变体的MetE生物体可以使用例如二甲基二硫醚（DMDS）的甲基封端硫化物源作为 硫和甲基，绕过MetH / MetE和MetF活性的需要，需要还原硫酸盐，用于合成甲硫氨酸。 该专利中还描述的是涉及MetY（也称为MetZ）作为将甲基封端的硫化物源例如DMDS掺入甲硫氨酸的酶的数据。 A＆Dgr; metF＆Dgr;谷氨酸棒杆菌的metB菌株可以使用甲基封端的硫化物源，例如DMDS，作为硫化物和甲基的来源。 此外，通过加入甲基封端的硫化物源（例如DMDS），改善过量产生O-乙酰高丝氨酸的工程化原养殖生物体的甲硫氨酸生产。