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    THERMOSTABLE L-RIBOSE ISOMERASE AND METHOD FOR PRODUCING SAME AND USE OF SAME
    4.
    发明申请
    THERMOSTABLE L-RIBOSE ISOMERASE AND METHOD FOR PRODUCING SAME AND USE OF SAME 有权
    可热裂解的L-赖氨酸异构体及其生产方法及其用途

    公开(公告)号：US20090325231A1

    公开(公告)日：2009-12-31

    申请号：US12515596

    申请日：2007-03-26

    申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    IPC分类号： C12P21/00 ,  C12N9/90 ,  C12N1/21 ,  C12P19/24

    CPC分类号： C12N9/90 ,  C12P19/24 ,  C12R1/01

    摘要： Object: To provide a thermostable L-ribose isomerase.Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1) Raoultella ornithinolytica strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.

    摘要翻译： 目的：提供热稳定的L-核糖异构酶。 解决方法：具有MW的热稳定的L-核糖异构酶。 32,000（通过SDS-PAGE），最适温度为45℃，pH 9.0的最佳pH（甘氨酸 - NaOH缓冲液），以及稳定的物理化学性质，例如在pH 9.0的热处理期间温度稳定性高达45℃，为10 分钟，并且具有异构化L-核糖以产生L-核酮糖的作用或者与L-核酮糖异构化以产生L-核糖的作用。 醛糖和酮糖之间的转化方法，包括使热稳定的L-核糖异构酶作为源自（1）鸟氨酸解脂耶氏杆菌菌株MB426（NITE BP-277）的酶与选自L-核糖，D-赖索糖， D-山梨糖醇，D-甘露糖，L-丙氨酸和L-古洛糖醇以使醛糖异构化以产生选自单独对应的L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵芝蛋白和L- 山梨糖或与选自L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵敏糖和L-山梨糖的酮糖相互作用以使酮糖异构化以产生选自相应的L-核糖，D D-果糖，D-甘露糖，D-甘露糖，L-阿洛糖和L-古洛糖。

    Microorganism with ability to produce deoxy polyol dehydrogenase and use thereof
    5.
    发明授权
    Microorganism with ability to produce deoxy polyol dehydrogenase and use thereof 有权
    具有生产脱氧多元醇脱氢酶能力的微生物及其用途

    公开(公告)号：US08530222B2

    公开(公告)日：2013-09-10

    申请号：US12515602

    申请日：2007-03-27

    申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    IPC分类号： C12N1/20

    CPC分类号： C12R1/01 ,  A61K8/60 ,  A61K2800/86 ,  A61Q19/00 ,  C12N9/0006 ,  C12P19/02

    摘要： Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase.Means for Resolution: A microorganism belonging to genus Enterobacter with an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol. The bacterial cell IK7 of the genus Enterobacter (accession No. NITE P-271). A method for producing deoxy ketose comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by the culturing of the microorganism of the invention or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol to oxidize deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose. The deoxy polyol is 1-deoxy-D-allitol, while the corresponding deoxy ketose is 1-deoxy-L-psicose. Otherwise, the deoxy polyol is L-rhamnitol, while the corresponding deoxy ketose is 1-deoxy-L-fructose.

    摘要翻译： 问题：提供具有生产脱氧多元醇脱氢酶能力的微生物。 解决方法：属于肠杆菌属的微生物，其在位置C2和C3处产生与核糖醇或L-阿托糖醇相同结构的脱氧多羟基化脱氢酶的能力。 肠杆菌属细菌细胞IK7（登录号NITE P-271）。 一种生产脱氧酮糖酮的方法，包括使含有通过培养本发明的微生物获得的脱氧多元醇脱氢酶的培养物或使脱氧多元醇脱氢酶与位置C2和C3处于相同结构的含有脱氧多羟基化合物的溶液反应，作为 核糖醇或L-天冬氨酸氧化脱氧多元醇以产生相应的脱氧酮糖，然后收集脱氧酮糖酮。 脱氧多元醇是1-脱氧-D-多糖，而相应的脱氧酮基是1-脱氧-L-精氨酸。 否则，脱氧多元醇为L-麦芽糖醇，而相应的脱氧酮糖为1-脱氧-L-果糖。

    Deoxyketohexose isomerase and method for producing deoxyhexose and derivative thereof using same
    6.
    发明授权
    Deoxyketohexose isomerase and method for producing deoxyhexose and derivative thereof using same 有权
    脱氧己糖异构酶及使用其脱氧己糖及其衍生物的方法

    公开(公告)号：US08389248B2

    公开(公告)日：2013-03-05

    申请号：US12515605

    申请日：2007-11-20

    申请人： Ken Izumori ,  Masaaki Tokuda ,  George Fleet ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    发明人： Ken Izumori ,  Masaaki Tokuda ,  George Fleet ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    IPC分类号： C12P19/24 ,  C12N9/90

    CPC分类号： C07H3/02 ,  C12N9/92 ,  C12P19/24

    摘要： Providing 1- or 6-deoxy products corresponding to all of aldohexoses, ketohexoses and sugar alcohols, as based on Deoxy-Izumoring, as well as a method for systematically producing those products. A method for producing deoxyketohexose and a derivative thereof using a deoxyketohexose isomerase derived from Pseudomonas cichorii ST-24 (FERM BP-2736), comprising epimerizing 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose at position 3 to produce the individually corresponding 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose as an intended product.

    摘要翻译： 提供对应于所有醛脱水己酮糖，酮己立糖和糖醇的1-脱氧产物或6-脱氧产物，以脱氧消肿为基础，以及一种系统地生产这些产品的方法。 使用衍生自奇异假单胞菌ST-24（FERM BP-2736）的脱氧己糖异构酶生产脱氧己糖及其衍生物的方法，其包括将1-脱氧D-酮己糖或6-脱氧D-酮己糖或1-脱氧L-酮己糖或 6-脱氧L-酮基己糖，以产生单独相应的1-脱氧D-酮己糖或6-脱氧D-酮己糖或1-脱氧L-酮己糖或6-脱氧L-酮己糖作为目标产物。

    Thermostable L-ribose isomerase and method for producing same and use of same
    7.
    发明授权
    Thermostable L-ribose isomerase and method for producing same and use of same 有权
    热稳定性L-核糖异构酶及其制备方法及用途

    公开(公告)号：US08227232B2

    公开(公告)日：2012-07-24

    申请号：US12515596

    申请日：2007-03-26

    申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    IPC分类号： C12N9/90 ,  C12N1/00 ,  C12P19/24 ,  C07H1/00 ,  C07H1/06

    CPC分类号： C12N9/90 ,  C12P19/24 ,  C12R1/01

    摘要： Object: To provide a thermostable L-ribose isomerase.Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45° C., optimal pH of pH 9.0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45° C. during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1) Raoultella ornithinolytica strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.

    摘要翻译： 目的：提供热稳定的L-核糖异构酶。 解决方法：具有MW的热稳定性L-核糖异构酶。 32,000（通过SDS-PAGE），最适温度为45℃，pH 9.0的最佳pH（甘氨酸 - NaOH缓冲液），以及稳定的物理化学性质，例如在pH 9.0的热处理期间温度稳定性高达45℃，为10 分钟，并且具有异构化L-核糖以产生L-核酮糖的作用或者与L-核酮糖异构化以产生L-核糖的作用。 醛糖和酮糖之间的转化方法，包括使热稳定的L-核糖异构酶作为源自（1）鸟氨酸解脂耶氏杆菌菌株MB426（NITE BP-277）的酶与选自L-核糖，D-赖索糖， D-山梨糖醇，D-甘露糖，L-丙氨酸和L-古洛糖醇以使醛糖异构化以产生选自单独对应的L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵芝蛋白和L- 山梨糖或与选自L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵敏糖和L-山梨糖的酮糖相互作用以使酮糖异构化以产生选自相应的L-核糖，D D-果糖，D-甘露糖，D-甘露糖，L-阿洛糖和L-古洛糖。

    MICROORGANISM WITH ABILITY TO PRODUCE DEOXY POLYOL DEHYDROGENASE AND USE THEREOF
    8.
    发明申请
    MICROORGANISM WITH ABILITY TO PRODUCE DEOXY POLYOL DEHYDROGENASE AND USE THEREOF 有权
    具有生产脱氧多糖脱氢酶能力的微生物及其用途

    公开(公告)号：US20100167350A1

    公开(公告)日：2010-07-01

    申请号：US12515602

    申请日：2007-03-27

    申请人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    发明人： Ken Izumori ,  Kenji Morimoto ,  Goro Takata ,  Masaaki Tokuda ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    IPC分类号： C12P19/00 ,  C07H3/08 ,  C12N1/20

    CPC分类号： C12R1/01 ,  A61K8/60 ,  A61K2800/86 ,  A61Q19/00 ,  C12N9/0006 ,  C12P19/02

    摘要： Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase.Means for Resolution: A microorganism belonging to genus Enterobacter with an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol. The bacterial cell IK7 of the genus Enterobacter (accession No. NITE P-271). A method for producing deoxy ketose comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by the culturing of the microorganism of the invention or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol to oxidize deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose. The deoxy polyol is 1-deoxy-D-allitol, while the corresponding deoxy ketose is 1-deoxy-L-psicose. Otherwise, the deoxy polyol is L-rhamnitol, while the corresponding deoxy ketose is 1-deoxy-L-fructose.

    摘要翻译： 问题：提供具有生产脱氧多元醇脱氢酶能力的微生物。 解决方法：属于肠杆菌属的微生物，其在位置C2和C3处产生与核糖醇或L-阿托糖醇相同结构的脱氧多羟基化脱氢酶的能力。 肠杆菌属细菌细胞IK7（登录号NITE P-271）。 一种生产脱氧酮糖酮的方法，包括使含有通过培养本发明的微生物获得的脱氧多元醇脱氢酶的培养物或使脱氧多元醇脱氢酶与位置C2和C3处于相同结构的含有脱氧多羟基化合物的溶液反应，作为 核糖醇或L-天冬氨酸氧化脱氧多元醇以产生相应的脱氧酮糖，然后收集脱氧酮糖酮。 脱氧多元醇是1-脱氧-D-多糖，而相应的脱氧酮基是1-脱氧-L-精氨酸。 否则，脱氧多元醇为L-麦芽糖醇，而相应的脱氧酮糖为1-脱氧-L-果糖。

    DEOXYKETOHEXOSE ISOMERASE AND METHOD FOR PRODUCING DEOXYHEXOSE AND DERIVATIVE THEREOF USING SAME
    9.
    发明申请
    DEOXYKETOHEXOSE ISOMERASE AND METHOD FOR PRODUCING DEOXYHEXOSE AND DERIVATIVE THEREOF USING SAME 有权
    脱乙酰壳多糖异构体及其制造方法及其衍生物

    公开(公告)号：US20100105885A1

    公开(公告)日：2010-04-29

    申请号：US12515605

    申请日：2007-11-20

    申请人： Ken Izumori ,  Masaaki Tokuda ,  George Fleet ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    发明人： Ken Izumori ,  Masaaki Tokuda ,  George Fleet ,  Yoshio Tsujisaka ,  Kei Takeshita ,  Keiji Tsusaki ,  Kazuhiro Okuma

    IPC分类号： C07H3/02 ,  C12N9/90 ,  C12P19/24

    CPC分类号： C07H3/02 ,  C12N9/92 ,  C12P19/24

    摘要： Providing 1- or 6-deoxy products corresponding to all of aldohexoses, ketohexoses and sugar alcohols, as based on Deoxy-Izumoring, as well as a method for systematically producing those products. A method for producing deoxyketohexose and a derivative thereof using a deoxyketohexose isomerase derived from Pseudomonas cichorii ST-24 (FERM BP-2736), comprising epimerizing 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose at position 3 to produce the individually corresponding 1-deoxy D-ketohexose or 6-deoxy D-ketohexose or 1-deoxy L-ketohexose or 6-deoxy L-ketohexose as an intended product.

    摘要翻译： 提供对应于所有醛脱水己酮糖，酮己立糖和糖醇的1-脱氧产物或6-脱氧产物，以脱氧消肿为基础，以及一种系统地生产这些产品的方法。 使用衍生自奇异假单胞菌ST-24（FERM BP-2736）的脱氧己糖异构酶生产脱氧己糖及其衍生物的方法，其包括将1-脱氧D-酮己糖或6-脱氧D-酮己糖或1-脱氧L-酮己糖或 6-脱氧L-酮基己糖，以产生单独相应的1-脱氧D-酮己糖或6-脱氧D-酮己糖或1-脱氧L-酮己糖或6-脱氧L-酮己糖作为目标产物。

    SWEETENER CONTAINING D-PSICOSE AND FOODS AND DRINKS OBTAINED BY USING THE SAME
    10.
    发明申请
    SWEETENER CONTAINING D-PSICOSE AND FOODS AND DRINKS OBTAINED BY USING THE SAME 审中-公开
    含有D-PSICOSE的食物和使用它的食物和饮料

    公开(公告)号：US20090304891A1

    公开(公告)日：2009-12-10

    申请号：US12514216

    申请日：2007-03-26

    申请人： Hideki Fujihara ,  Kazuhiro Okuma ,  Tetsuo Iida ,  Ken Izumori ,  Masaaki Tokuda ,  Kazuhiro Fukuda

    发明人： Hideki Fujihara ,  Kazuhiro Okuma ,  Tetsuo Iida ,  Ken Izumori ,  Masaaki Tokuda ,  Kazuhiro Fukuda

    IPC分类号： A23L1/236

    CPC分类号： A23C9/13 ,  A23G3/36 ,  A23G3/42 ,  A23G4/06 ,  A23G4/10 ,  A23L2/60 ,  A23L27/33 ,  A61K8/60 ,  A61K47/26 ,  A61Q11/00

    摘要： Providing a D-psicose-containing sweetener with the modification of the taste of D-psicose, comprising D-psicose, a sugar alcohol and/or a high intensity sweetener, preferably containing D-psicose as the main component, particularly a low-calorie sweetener and/or a sweetener giving refreshing feel in the oral cavity, as well as foods and drinks obtained by using the D-psicose-containing sweetener with the modification of the taste of D-psicose, and other products given with sweetness. The sugar alcohol is one or more sugar alcohols selected from the group consisting of sorbitol, mannitol, lactitol, maltitol, xylitol and erythritol, while the high intensity sweetener is one or more high intensity sweeteners as selected from aspartame, acesulfame K, sodium cyclamate, sodium saccharin, Sucralose (under trade name), stevia sweetener, dulcin, taumatin, neotame and monellin.

    摘要翻译： 提供具有D-灵糖蛋白的甜味剂，其具有改性的D-psicose的味道，包括D-肉豆蔻糖，糖醇和/或高强度甜味剂，优选含有D-灵敏蛋白作为主要成分，特别是低热量 甜味剂和/或甜味剂在口腔中提供清爽的感觉，以及通过使用含有D-psicose的甜味剂获得的食品和饮料以及D-psicose的味道的改变以及其它赋予甜味的产品。 糖醇是选自山梨糖醇，甘露糖醇，乳糖醇，麦芽糖醇，木糖醇和赤藓糖醇的一种或多种糖醇，而高强度甜味剂是选自阿斯巴甜，乙酰磺胺酸钾，环己烷氨基磺酸钠的一种或多种高强度甜味剂， 糖精钠，三氯蔗糖（商品名），甜叶菊甜味剂，葫芦素，桃黄素，纽甜和莫奈林。