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    Method of genetic testing
    1.
    发明申请
    Method of genetic testing 审中-公开
    基因检测方法

    公开(公告)号：US20050287549A1

    公开(公告)日：2005-12-29

    申请号：US11041456

    申请日：2005-01-25

    申请人： Keiichi Nagai ,  Kazunori Okano ,  Hideyuki Noda ,  Hiroko Matsunaga ,  Kiyomi Taniguchi ,  Yoshiaki Yazawa ,  Tomoharu Kajiyama

    发明人： Keiichi Nagai ,  Kazunori Okano ,  Hideyuki Noda ,  Hiroko Matsunaga ,  Kiyomi Taniguchi ,  Yoshiaki Yazawa ,  Tomoharu Kajiyama

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6837 ,  C12Q2565/537 ,  C12Q2565/301 ,  C12Q2525/155

    摘要： This invention provides a method of genetic testing that enables testing of a plurality of variation sites (SNPs) in a cost-effective and simple manner, allowing realization of genetic diagnosis in clinical settings. The SNP type of the nucleic acid sample is evaluated by: allowing a nucleic acid sample having an anchor sequence at its 5′ end to hybridize to a support having, immobilized on its surface, a probe containing a sequence that is complementary to the target sequence (the SNP region); extending a complementary strand from the probe utilizing the nucleic acid sample as a template; dissociating and removing the nucleic acid sample from the extended probe; extending a complementary strand using the extended probe as a template and a primer having a sequence identical to the anchor sequence; and detecting pyrophosphoric acid generated via the primer extension, based on bioluminescence.

    摘要翻译： 本发明提供了一种能够以成本有效和简单的方式测试多个变异位点（SNP）的遗传测试方法，从而允许在临床环境中实现遗传诊断。 核酸样品的SNP类型通过以下方式评估：使具有其5'端的锚定序列的核酸样品与固定在其表面上的载体杂交，所述载体固定有包含与靶序列互补的序列的探针 （SNP地区）; 使用核酸样品作为模板从探针延伸互补链; 从扩展的探针中分离并除去核酸样品; 使用扩展探针作为模板和具有与锚定序列相同的序列的引物延伸互补链; 并基于生物发光检测通过引物延伸产生的焦磷酸。

    Method for nucleic acid analysis
    2.
    发明申请
    Method for nucleic acid analysis 审中-公开
    核酸分析方法

    公开(公告)号：US20060223087A1

    公开(公告)日：2006-10-05

    申请号：US11342861

    申请日：2006-01-31

    申请人： Kiyomi Taniguchi ,  Keiichi Nagai ,  Hiroko Matsunaga

    发明人： Kiyomi Taniguchi ,  Keiichi Nagai ,  Hiroko Matsunaga

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/683 ,  C12Q2537/113 ,  C12Q2523/125 ,  C12Q2521/307

    摘要： A simple and highly accurate method for detecting the presence or absence of gene mutation and methylated cytosine in CpG dinucleotide that are contained in a target sequence derived from an analysis sample is provided. Features of the method for nucleic acid analysis include cleaving one or more noncomplementary sites in a double-stranded nucleic acid sample by a single strand-specific endonuclease, hybridizing at least one of the nucleic acid fragments obtained to a probe containing a nucleotide sequence that is partially or totally identical to either one strand of the double-stranded nucleic acid sample, allowing an extension reaction to proceed from the nucleic acid fragment hybridized to the probe, and optically detecting pyrophosphate generated by the extension reaction, thereby judging the presence or absence of at least a noncomplementary site in the double-stranded nucleic acid sample.

    摘要翻译： 提供了一种用于检测来自分析样品的靶序列中包含的CpG二核苷酸中基因突变和甲基化胞嘧啶的存在或不存在的简单且高度准确的方法。 用于核酸分析的方法的特征包括通过单链特异性内切核酸酶切割双链核酸样品中的一个或多个非互补位点，将获得的核酸片段中的至少一个与包含核苷酸序列的探针杂交 部分或全部与双链核酸样品的一条链相同，允许从与探针杂交的核酸片段进行延伸反应，并光学检测由延伸反应产生的焦磷酸盐，从而判断是否存在 至少在双链核酸样品中的非互补位点。

    Container for suspension and filtration of stool
    3.
    发明申请
    Container for suspension and filtration of stool 审中-公开
    用于粪便悬浮和过滤的容器

    公开(公告)号：US20060122534A1

    公开(公告)日：2006-06-08

    申请号：US11212575

    申请日：2005-08-29

    申请人： Yasuhiro Matsumura ,  Hisayuki Matsushita ,  Hiroyuki Tsunoda ,  Kunio Harada ,  Kazunori Okano ,  Keiichi Nagai

    发明人： Yasuhiro Matsumura ,  Hisayuki Matsushita ,  Hiroyuki Tsunoda ,  Kunio Harada ,  Kazunori Okano ,  Keiichi Nagai

    IPC分类号： A61B10/00 ,  A61B5/00

    CPC分类号： A61B10/0038 ,  A61B10/0096

    摘要： A container for the suspension and filtration of stool enables quick, simple, and safe collection of cancer cells separated in stool. The container comprises (a) a stool collection container 1, (c) a stool processing container main body 20, and (d) a pushing member 30. The stool collection container 1 comprises a syringe 2 capable of collecting 0.5 g or more of stool by being thrust into stool, a stool collecting opening, a handle 3 provided on the periphery of the syringe at the opposite end to the stool collecting opening, and a cap member 4 provided at the opposite end to the stool collecting opening. The stool processing container main body 20 comprises: a syringe storage portion 21 for storing the syringe; a suspension portion 22 connected to the syringe storage portion for suspending the stool; a filtrate receiving container 23 detachably connected to the suspension portion for receiving a filtrate of the stool that has been suspended and filtered; and a filter 26 provided at a connection portion between the suspension portion and the filtrate receiving container. The pushing member 30 is pressed to press or tear the cap member so as to move the stool collected in the syringe of the stool collection container into the suspension portion.

    摘要翻译： 用于粪便悬浮和过滤的容器能够快速，简单和安全地收集在粪便中分离的癌细胞。 容器包括（a）粪便收集容器1，（c）粪便处理容器主体20，和（d）推动构件30。 粪便收集容器1包括注射器2，其能够通过推入粪便中收集0.5g或更多的粪便，粪便收集开口，在与粪便收集口相对的端部处设置在注射器的周边上的手柄3，以及 盖构件4设置在与粪便收集口相对的另一端。 粪便处理容器主体20包括：用于储存注射器的注射器存放部21; 连接到注射器存储部分用于悬挂粪便的悬挂部分22; 可拆卸地连接到悬挂部分的滤液接收容器23，用于接收已经悬浮和过滤的粪便滤液; 以及设置在悬架部分和滤液接收容器之间的连接部分处的过滤器26。 按压构件30被按压或撕开盖构件，以将收集在粪便收集容器的注射器中的粪便移动到悬挂部分中。

    DNA analyzing method
    5.
    发明授权
    DNA analyzing method 失效
    DNA分析方法

    公开(公告)号：US5824481A

    公开(公告)日：1998-10-20

    申请号：US812698

    申请日：1997-03-06

    申请人： Hideki Kambara ,  Kazunori Okano ,  Satoshi Takahashi ,  Keiichi Nagai ,  Kazuko Kawamoto ,  Hiroko Furuyama

    发明人： Hideki Kambara ,  Kazunori Okano ,  Satoshi Takahashi ,  Keiichi Nagai ,  Kazuko Kawamoto ,  Hiroko Furuyama

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q1/6846

    摘要： A DNA analyzing method which bonds a first oligomer of known base sequence to a DNA fragment obtained by digesting a DNA sample with a restrictive enzyme. The oligomer and DNA fragment are hybridized to other oligomers which have the sequences of all combinations of the types of bases within the length of several bases following the known base sequence. The presence or absence of hybridization or complementary DNA strand extension is determined and identifies the DNA fragment terminal sequence from this result. The DNA fragments are then fractionated and analyzed to determine the sequence. This DNA analyzing method provides an effective analysis of mixtures of long DNAs or DNA fragments.

    摘要翻译： 将已知碱基序列的第一寡聚体与限制性酶消化DNA样品获得的DNA片段结合的DNA分析方法。 寡聚体和DNA片段与其它寡核苷酸杂交，其具有在已知碱基序列之后的几个碱基长度内的碱基类型的所有组合的序列。 确定是否存在杂交或互补DNA链延伸，并从该结果鉴定DNA片段末端序列。 然后将DNA片段分级并分析以确定序列。 该DNA分析方法提供了长DNA或DNA片段的混合物的有效分析。

    DNA measuring method
    6.
    发明授权
    DNA measuring method 失效
    DNA测定方法

    公开(公告)号：US5356776A

    公开(公告)日：1994-10-18

    申请号：US942470

    申请日：1992-09-09

    申请人： Hideki Kambara ,  Kazunori Okano ,  Satoshi Takahashi ,  Keiichi Nagai ,  Tetsuo Nishikawa

    发明人： Hideki Kambara ,  Kazunori Okano ,  Satoshi Takahashi ,  Keiichi Nagai ,  Tetsuo Nishikawa

    IPC分类号： C12M1/00 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/70

    CPC分类号： G01N27/44726 ,  C12Q1/68 ,  G01N27/447 ,  Y10T436/143333

    摘要： DNA molecule length can be measured with high precision and efficiency by 1) using such means as electrophoresis gel migration to orient a DNA molecule having a fluorescence label at both its termini into a straight line by its passing through a migration path having in a portion of it an area not more than several micrometers in diameter, detecting the fluorescence label at both the termini at a predetermined location and measuring the interval between the detection of the fluorescence coming from one terminus and that of the fluorescence from the other or by 2) a DNA molecule bound to a fluorescence label at one terminus and to a particle at the other being led as a whole by such means as electric field application into an aperture smaller in diameter than the particle, leaving the particle fixed at the mouth of the aperture to stretch the DNA molecule and detecting the fluorescence position to measure the distance between the bound particle and the bound fluorescence label.

    摘要翻译： 可以通过以下方式测量DNA分子长度：1）使用电泳凝胶迁移的方式，通过其经过一部分迁移路径将具有两端的荧光标记的DNA分子定向成直线 它是直径不超过几微米的区域，在预定位置的两个末端检测荧光标记，并测量从一个末端检测荧光和来自另一个末端的荧光检测之间的间隔，或者通过2）a 在一个末端与一个荧光标记结合的DNA分子和另一个的一个粒子整体通过电场施加到比颗粒直径更小的孔隙中，将颗粒固定在孔的口处 拉伸DNA分子并检测荧光位置以测量结合的颗粒和结合的荧光标记之间的距离。

    DNA analyzing method
    7.
    发明授权
    DNA analyzing method 失效
    DNA分析方法

    公开(公告)号：US5650274A

    公开(公告)日：1997-07-22

    申请号：US263663

    申请日：1994-06-22

    申请人： Hideki Kambara ,  Kazunori Okano ,  Satoshi Takahashi ,  Keiichi Nagai ,  Kazuko Kawamoto ,  Hiroko Furuyama

    发明人： Hideki Kambara ,  Kazunori Okano ,  Satoshi Takahashi ,  Keiichi Nagai ,  Kazuko Kawamoto ,  Hiroko Furuyama

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/70

    CPC分类号： C12Q1/6869 ,  C12Q1/6846

    摘要： A DNA analyzing method comprising ligating the oligomer of a known base sequence to the DNA fragment obtained by digesting a sample with a restriction enzyme, hybridizing an oligomer and DNA fragment using oligomers which have the sequences of all combinations of the types of the bases within the length of several bases following the known base sequence, checking the presence or absence of hybridization or complementary DNA strand extension, identifying the DNA fragment terminal sequence from this result, and fractionating the DNA fragments and analyzing them. This DNA analyzing method provides an effective analysis of mixtures of long DNAs or DNA fragments.

    摘要翻译： 一种DNA分析方法，包括将已知碱基序列的寡聚物与通过用限制酶消化样品获得的DNA片段连接，使用寡核苷酸与低聚物和DNA片段杂交，所述寡聚体具有碱基类型的所有组合的序列 根据已知碱基序列的几个碱基的长度，检查杂交或互补DNA链延伸的存在或不存在，从该结果鉴定DNA片段末端序列，并分离DNA片段并分析它们。 该DNA分析方法提供了长DNA或DNA片段的混合物的有效分析。

    Method for testing nucleic acid sequence
    8.
    发明授权
    Method for testing nucleic acid sequence 失效
    核酸序列测试方法

    公开(公告)号：US06821734B2

    公开(公告)日：2004-11-23

    申请号：US10083340

    申请日：2002-02-27

    申请人： Hideki Kambara ,  Guohua Zhou ,  Kazunori Okano ,  Keiichi Nagai

    发明人： Hideki Kambara ,  Guohua Zhou ,  Kazunori Okano ,  Keiichi Nagai

    IPC分类号： C12Q168

    CPC分类号： C12Q1/682 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2565/537 ,  C12Q2561/125 ,  C12Q2531/125 ,  C12Q2525/301 ,  C12Q2565/301 ,  C12Q2565/519

    摘要： A method for examining nucleotide sequences of a sample includes adding a group of primers of multiple species to a solution containing the sample and simultaneously synthesizing complementary strands at each of the multiple regions containing the nucleotide sequences; designing the DNA probes with specific sequences elongate the complementary strands by the presence or absence of mutations in the nucleotide sequences, wherein the same number of such DNA probes and the nucleotide sequences are used for complementary strand synthesis; using the nucleotide sequences or their complementary sequences as a template to convert pyrophosphate produced during the elongation reaction to ATP which then reacts with chemiluminescent substrates to develop luminescence to be detected. According to the method, sensitivity is greatly increased by amplification of the amount of pyrophosphate produced in synthesis of the complementary strands without amplifying the copies of nucleotide sequences.

    摘要翻译： 检查样品的核苷酸序列的方法包括将一组多种引物添加到含有样品的溶液中，同时在含有该核苷酸序列的多个区域的每个区域合成互补链; 用特定序列设计DNA探针，通过核苷酸序列中存在或不存在突变来延长互补链，其中相同数量的这种DNA探针和核苷酸序列用于互补链合成; 使用核苷酸序列或其互补序列作为模板将在延伸反应期间产生的焦磷酸转化为ATP，然后与化学发光底物反应以产生待检测的发光。 根据该方法，在不扩增核苷酸序列的拷贝的情况下，通过扩增合成互补链中产生的焦磷酸盐的量来大大提高灵敏度。

    METHOD OF ANALYZING NUCLEIC ACID
    9.
    发明申请
    METHOD OF ANALYZING NUCLEIC ACID 审中-公开
    分析核酸的方法

    公开(公告)号：US20100173287A1

    公开(公告)日：2010-07-08

    申请号：US12090701

    申请日：2006-03-07

    申请人： Yukie Nakashima ,  Keiichi Nagai

    发明人： Yukie Nakashima ,  Keiichi Nagai

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q1/6839 ,  C12Q2537/119 ,  C12Q2521/501

    摘要： According to the present invention, stable amplification of a small amount of nucleic acid and analysis of the same with good sensitivity can be realized by improving efficiency of hybridization primers or probes with a probe. Specifically, the present invention relates to a method of analyzing nucleic acid comprising: a step of hybridizing at least one type of a first probe comprising a 1st sequence complementary to one strand of double-strand nucleic acid, a 2nd sequence complementary to the other strand thereof with the double-strand nucleic acid, and a 3rd sequence that binds the 1st sequence and the 2nd sequence; and a step of hybridizing at least one type of a second probe with the double-strand nucleic acid.

    摘要翻译： 根据本发明，通过用探针提高杂交引物或探针的效率，可以实现少量核酸的稳定扩增及其灵敏度的分析。 具体地说，本发明涉及分析核酸的方法，包括：将至少一种类型的第一探针杂交的步骤，所述第一探针包含与一条双链核酸互补的第一序列，与另一条链互补的第二序列 与第二序列和第二序列结合的第三序列; 以及使至少一种类型的第二探针与双链核酸杂交的步骤。