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    Cell separation device using ultrasound and electrophoresis
    1.
    发明授权
    Cell separation device using ultrasound and electrophoresis 失效
    细胞分离装置采用超声波和电泳

    公开(公告)号：US06245207B1

    公开(公告)日：2001-06-12

    申请号：US09313212

    申请日：1999-05-18

    申请人： Kenji Yasuda ,  Takeshi Sakamoto

    发明人： Kenji Yasuda ,  Takeshi Sakamoto

    IPC分类号： C02F140

    CPC分类号： B01D21/283 ,  B01D21/0009 ,  B03D3/04 ,  C02F1/36 ,  C02F1/469 ,  C12M47/04 ,  G01N27/44769

    摘要： A cell separation module for separating and collecting cells having a charge and cells having no charge comprises a holder and a throwaway cell separation chamber held by the holder. Ultrasound is introduced into the cell separation chamber so as to allow force for causing cells to be put together to act into the cell separation chamber. In addition, an electrostatic force is allowed to act in the cell separation chamber so as to cause the cell to move by the charges of the cells. Competition between both the forces is used to separate the cells correspondingly to the freshness of the cells.

    摘要翻译： 用于分离和收集具有电荷的细胞和不含电荷的细胞的细胞分离模块包括保持器和由保持器保持的一次性细胞分离室。 将超声波引入细胞分离室，以便允许将细胞置于一起作用于细胞分离室的力。 此外，允许静电力作用于细胞分离室，以使细胞通过细胞的电荷而移动。 使用两个力之间的竞争来对应于细胞的新鲜度分离细胞。

    Particle concentration device
    2.
    发明授权
    Particle concentration device 失效
    粒子浓度仪

    公开(公告)号：US06273262B1

    公开(公告)日：2001-08-14

    申请号：US09317608

    申请日：1999-05-25

    申请人： Kenji Yasuda ,  Takeshi Sakamoto

    发明人： Kenji Yasuda ,  Takeshi Sakamoto

    IPC分类号： B03B566

    CPC分类号： B01D21/283 ,  G01N1/40 ,  G01N29/223 ,  G01N2291/02416

    摘要： A device comprises a means for radiating ultrasound from one face of a chamber holding a sample solution containing particles to be concentrated, and a reflective face inclined to the face irradiated with the ultrasound. The frequency of the ultrasound radiated from the ultrasound radiating means is changed periodically and asymmetrically with the passage of time.

    摘要翻译： 一种装置包括用于从保持含有待浓缩颗粒的样品溶液的室的一个面的一个面辐射超声波的装置和与被超声波照射的面倾斜的反射面。 从超声波辐射装置发射的超声波的频率随着时间的推移而周期性地和不对称地改变。

    Method and apparatus for testing cardiotoxicity and evaluating cardiomyocytes
    4.
    发明授权
    Method and apparatus for testing cardiotoxicity and evaluating cardiomyocytes 有权
    用于测试心脏毒性和评估心肌细胞的方法和装置

    公开(公告)号：US09447447B2

    公开(公告)日：2016-09-20

    申请号：US13876992

    申请日：2011-09-30

    申请人： Kenji Yasuda ,  Tomoyuki Kaneko ,  Fumimasa Nomura

    发明人： Kenji Yasuda ,  Tomoyuki Kaneko ,  Fumimasa Nomura

    IPC分类号： C12M1/34 ,  C12Q1/02 ,  C12M1/42 ,  C12M1/36 ,  G01N33/50

    CPC分类号： C12Q1/025 ,  C12M35/02 ,  C12M41/46 ,  C12M41/48 ,  G01N33/48728 ,  G01N33/5061 ,  G01N33/5088 ,  G01N2800/32

    摘要： In the present invention, a cardiomyocyte cluster is disposed on a transparent substrate, and the quality of the cardiomyocytes is evaluated from the response of the cells to a forced pulsation stimulus applied to the cardiomyocytes. The cardiomyocyte cluster is disposed on the transparent substrate, and is exposed to the flow of a liquid containing an agent in a manner so that the agent acts on the cells, which configure a network. The extent of cardiac toxicity resulting from the agent is evaluated from measuring the fluctuations obtained from a comparison of adjacent cardiomyocytes of the network.

    摘要翻译： 在本发明中，将心肌细胞簇设置在透明基质上，根据细胞对施加于心肌细胞的强制脉动刺激的反应来评价心肌细胞的质量。 将心肌细胞簇设置在透明基板上，并且以使得试剂作用于构成网络的细胞的方式暴露于含有试剂的液体的流动。 通过测量从网络的相邻心肌细胞的比较获得的波动来评估由该试剂产生的心脏毒性的程度。

    Cell measuring and sorting chip
    5.
    发明授权
    Cell measuring and sorting chip 有权
    电池测量和分选芯片

    公开(公告)号：US08361412B2

    公开(公告)日：2013-01-29

    申请号：US11317004

    申请日：2005-12-27

    申请人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    发明人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    IPC分类号： B01L3/00

    CPC分类号： G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

    摘要： A cell sorting device using an inexpensive chip capable of being exchanged for each sample. The chip includes: a first flow path allowing buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow buffer fluid not containing cells to flow down; a fourth flow path which allows the buffer fluid as a single flow path formed by joining the buffer fluids in the other three flow paths; a cell detecting region for detecting cells flowing with the buffer fluid down the fourth flow path; and a cell sorting region for sorting the cells according to a type of the cells detected. The first to fourth flow paths are cascaded, are supplied with the buffer fluid from reservoirs with the same fluid level, and have substantially the same width or cross-section area.

    摘要翻译： 一种使用廉价芯片的细胞分选装置，能够对每个样品进行交换。 芯片包括：允许包含电池的缓冲流体向下流动的第一流动路径; 第二和第三流动路径，其中第一流动路径在其间并且允许不包含电池的缓冲流体向下流动; 第四流路，其允许缓冲流体作为通过连接其它三个流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测沿着第四流动路径流动的缓冲液的细胞; 以及用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一至第四流动路径级联，从具有相同流体水平的储存器提供缓冲流体，并且具有基本上相同的宽度或横截面面积。

    Micro-chamber processing apparatus for cell culture and method
    6.
    发明授权
    Micro-chamber processing apparatus for cell culture and method 有权
    用于细胞培养和方法的微室处理装置

    公开(公告)号：US08247222B2

    公开(公告)日：2012-08-21

    申请号：US13036600

    申请日：2011-02-28

    申请人： Akihiro Hattori ,  Kenji Yasuda

    发明人： Akihiro Hattori ,  Kenji Yasuda

    IPC分类号： C12M1/00 ,  C12M1/34

    CPC分类号： C12M23/16 ,  B01J2219/00317 ,  B01J2219/00441 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00743 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/163

    摘要： This invention relates to a method and apparatus for processing cell cultures. The apparatus comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, the absorption layer having absorbency in visible and infrared regions, and the gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and the light source having a monochromatic light in the specific wave length, wherein the light source is disposed such that it irradiates on the absorption layer and/or the gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on the transparent base plate.

    摘要翻译： 本发明涉及一种用于处理细胞培养物的方法和装置。 该装置包括一个微室，该微室包括不超过一个吸收层和至少一个凝胶层，依次层叠在透明基板上，在可见光和红外区域中没有明显的吸收性，以及至少一个光源，吸收 在可见光和红外区域具有吸收性的层，凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物，在室温下处于凝胶状态，具有特定的吸光度 可见光和红外区域的波长，以及具有特定波长的单色光的光源，其中光源被设置成使其照射在吸收层和/或凝胶层上，除了当没有吸收 在透明基板上层叠由凝胶状材料构成的至少两层。

    Cell separation chip and cell culturing method using the same
    8.
    发明申请
    Cell separation chip and cell culturing method using the same 审中-公开
    细胞分离芯片和细胞培养方法使用相同

    公开(公告)号：US20080003678A1

    公开(公告)日：2008-01-03

    申请号：US11819681

    申请日：2007-06-28

    申请人： Akihiro Hattori ,  Hideyuki Terazono ,  Kenji Yasuda

    发明人： Akihiro Hattori ,  Hideyuki Terazono ,  Kenji Yasuda

    IPC分类号： C12N5/06 ,  C12M1/00

    CPC分类号： B01L3/502761 ,  B01L3/502753 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  C12M45/00 ,  C12M47/04 ,  G01N15/1031 ,  G01N15/12 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1484 ,  G01N2015/1081 ,  G01N2015/149

    摘要： An inexpensive cell analysis and separation apparatus using a flow channel formed on one surface of a substrate and a chip replaceable for each sample, and a method for culturing the separated cells without contamination, are provided. A flow channel for allowing a cell-containing buffer solution to flow is provided. Cells are detected in the middle of the flow channel, and separated to a plurality of downstream flow channels based on whether each cell fulfills a predetermined condition. A culturing tank for collecting the condition-fulfilling cells is covered with a semipermeable membrane at a top surface so as to prevent contamination during cell separation. When the cell separation is finished, the flow channel communicated with the culturing tank accommodating the condition-fulfilling cells is closed, and the culturing tank is cut off from the apparatus and put into a culturing device containing a predetermined medium to culture the cells.

    摘要翻译： 提供了使用形成在基板的一个表面上的流动通道和可替代每个样品的芯片的便宜的电池分析和分离装置，以及用于培养分离的电池而不被污染的方法。 提供了允许含细胞的缓冲溶液流动的流动通道。 基于每个小区是否满足预定条件，在流信道的中间检测小区，并将其分离成多个下游流信道。 用于收集状态满足细胞的培养槽在顶表面被半透膜覆盖，以防止细胞分离过程中的污染。 当细胞分离完成时，与容纳条件满足细胞的培养箱连通的流动通道关闭，并将培养箱从装置中切下并放入含有预定培养基的培养装置中以培养细胞。

    Method of inspecting a DNA chip
    9.
    发明授权
    Method of inspecting a DNA chip 有权
    检查DNA芯片的方法

    公开(公告)号：US07217573B1

    公开(公告)日：2007-05-15

    申请号：US09678652

    申请日：2000-10-04

    申请人： Yoshitada Oshida ,  Toshihiko Nakata ,  Tomoaki Sakata ,  Kenji Yasuda ,  Satoshi Takahashi

    发明人： Yoshitada Oshida ,  Toshihiko Nakata ,  Tomoaki Sakata ,  Kenji Yasuda ,  Satoshi Takahashi

    IPC分类号： G01N33/00 ,  G01N33/53 ,  C12Q1/68 ,  C12M3/00 ,  C07H21/04 ,  H01J3/14

    CPC分类号： G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  Y10S436/80 ,  Y10T436/143333

    摘要： A method of inspecting a DNA chip and an apparatus therefor that allow a picture to be reconstructed in the following steps: A plurality of irradiation spots are formed on a DNA probe array mounted on a stage. Then, the stage is displaced in X, Y directions so as to execute a scanning, thereby irradiating substantially all the entire surface of the DNA probe array. Next, a plurality of emitted fluorescent lights, which are generated from the plurality of irradiation spot portions on the DNA probe array, are converged and are then detected simultaneously by multi detectors. Finally, a data processing apparatus processes the detected signals, thereby reconstructing the picture.

    摘要翻译： 一种用于检查DNA芯片的方法及其装置，其允许以下列步骤重建图像：在安装在平台上的DNA探针阵列上形成多个照射点。 然后，在X，Y方向上移位台，以进行扫描，从而基本上照射DNA探针阵列的整个表面。 接下来，从DNA探针阵列上的多个照射点部分产生的多个发射荧光灯被会聚，然后由多个检测器同时检测。 最后，数据处理装置处理检测到的信号，从而重建图像。

    Cell measuring and sorting chip
    10.
    发明申请
    Cell measuring and sorting chip 有权
    电池测量和分选芯片

    公开(公告)号：US20060194307A1

    公开(公告)日：2006-08-31

    申请号：US11317004

    申请日：2005-12-27

    申请人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    发明人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    IPC分类号： C12M1/34 ,  B07C5/00

    CPC分类号： G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

    摘要： The present invention provides a cell sorting device using an inexpensive chip capable of being exchanged for each sample for cell sorting. The cell sorting device uses flow paths formed on the substrate and has the following structure. The chip includes: a first flow path allowing a buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow a buffer fluid not containing cells to flow down from both sides of the first flow path; a fourth flow path which allows the buffer fluid as a single flow path formed by the join of the buffer fluid in the first flow path and the buffer fluid in the second and third flow paths; a cell detecting region for detecting cells flowing with a buffer fluid down the fourth flow path; a cell sorting region for sorting the cells according to a type of the cells detected. The first flow path, the second and the third flow paths, the fourth flow path, the cell detecting region and the cell sorting region provided in a cascade. Two flow paths are provided to allow the cells sorted to flow downstream of the cell sorting region. The buffer fluid flowing in the first to the fourth flow paths is supplied from reservoirs having the same fluid level. The first flow path and the fourth flow path are structured to have substantially the same width or cross-section area.

    摘要翻译： 本发明提供一种使用廉价的芯片的细胞分选装置，其能够用于每个样品进行细胞分选。 细胞分选装置使用形成在基板上的流路，具有以下结构。 芯片包括：允许包含电池的缓冲流体向下流动的第一流路; 第二和第三流动路径，其将第一流动路径放置在其间并允许不含有电池的缓冲流体从第一流动路径的两侧向下流动; 第四流动路径，其允许缓冲流体作为由第一流动路径中的缓冲流体的连接和第二和第三流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测在第四流路下方以缓冲液流动的细胞; 用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一流路，第二流路，第三流路，第四流路，单元检测区域和电池排列区域。 提供了两个流动路径以允许排序的细胞流动到细胞分选区域的下游。 在第一至第四流动路径中流动的缓冲流体从具有相同流体液位的储存器供应。 第一流路和第四流路被构造成具有基本相同的宽度或横截面面积。