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    Multi-well microfiltration apparatus
    2.
    发明授权
    Multi-well microfiltration apparatus 有权
    多孔微滤装置

    公开(公告)号：US6159368A

    公开(公告)日：2000-12-12

    申请号：US182946

    申请日：1998-10-29

    申请人： Stephen E. Moring ,  Kevin S. Bodner ,  Jason H. Halsey ,  Jon Hoshizaki ,  Joseph Jackson ,  Alfred P. Madden ,  Mark F. Oldham ,  Mark T. Reed

    发明人： Stephen E. Moring ,  Kevin S. Bodner ,  Jason H. Halsey ,  Jon Hoshizaki ,  Joseph Jackson ,  Alfred P. Madden ,  Mark F. Oldham ,  Mark T. Reed

    IPC分类号： G01N1/00 ,  B01D29/00 ,  B01D35/02 ,  B01D53/22 ,  B01D61/18 ,  B01L3/00 ,  C12N15/09 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  B01D63/00

    CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  G01N35/0099 ,  B01L2400/049 ,  G01N2035/00485 ,  G01N2035/102 ,  G01N35/028 ,  G01N35/1074 ,  Y10T436/25

    摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with is the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

    摘要翻译： 本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。 在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器 板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。 本发明提供了相对简单的多孔微过滤装置，并且克服了许多与以下问题有关的问题：（i）由于穿过公共过滤片芯线而引起的交叉污染或（ii）单个过滤元件 在大量死体积内捕获样品成分。 此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。 另外，本发明提供了多孔微量过滤装置，其避免了由于气溶胶形成，悬挂液滴和/或飞溅而导致的交叉污染。 本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

    Multi-well microfiltration apparatus
    3.
    发明授权
    Multi-well microfiltration apparatus 有权
    多孔微滤装置

    公开(公告)号：US06338802B1

    公开(公告)日：2002-01-15

    申请号：US09565566

    申请日：2000-05-04

    申请人： Kevin S. Bodner ,  Alfred P. Madden ,  Joseph Jackson ,  Jason H. Halsey ,  Mark T. Reed ,  Ward Frye ,  Mark F. Oldham ,  Stephen E. Moring ,  Jon Hoshizaki

    发明人： Kevin S. Bodner ,  Alfred P. Madden ,  Joseph Jackson ,  Jason H. Halsey ,  Mark T. Reed ,  Ward Frye ,  Mark F. Oldham ,  Stephen E. Moring ,  Jon Hoshizaki

    IPC分类号： B01D6100

    CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102 ,  Y10T436/25

    摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

    摘要翻译： 本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。 在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器 板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。 本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件 大量死体积的样品成分。 此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。 此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。 本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

    Apparatus and method for avoiding cross-contamination due to pendent drops of fluid hanging from discharge conduits
    4.
    发明授权
    Apparatus and method for avoiding cross-contamination due to pendent drops of fluid hanging from discharge conduits 有权
    用于避免由排放管道悬挂的流体悬垂液滴引起的交叉污染的装置和方法

    公开(公告)号：US06783732B2

    公开(公告)日：2004-08-31

    申请号：US10199743

    申请日：2002-07-19

    申请人： Alfred P Madden ,  Jon Hoshizaki ,  Jason H. Halsey ,  Stephen E. Moring ,  Mark F. Oldham ,  Kevin S. Bodner

    发明人： Alfred P Madden ,  Jon Hoshizaki ,  Jason H. Halsey ,  Stephen E. Moring ,  Mark F. Oldham ,  Kevin S. Bodner

    IPC分类号： G01N3500

    CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102 ,  Y10T436/25

    摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

    摘要翻译： 本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。 在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器 板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。 本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件 大量死体积的样品成分。 此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。 此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。 本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

    Multi-well microfiltration apparatus and method for avoiding cross-contamination
    5.
    发明授权
    Multi-well microfiltration apparatus and method for avoiding cross-contamination 有权
    多孔微滤装置及避免交叉污染的方法

    公开(公告)号：US06506343B1

    公开(公告)日：2003-01-14

    申请号：US09565202

    申请日：2000-05-04

    申请人： Kevin S. Bodner ,  Alfred P. Madden ,  Jason H. Halsey ,  Mark F. Oldham ,  Stephen E. Moring ,  Jon Hoshizaki

    发明人： Kevin S. Bodner ,  Alfred P. Madden ,  Jason H. Halsey ,  Mark F. Oldham ,  Stephen E. Moring ,  Jon Hoshizaki

    IPC分类号： B01D3500

    CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102 ,  Y10T436/25

    摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

    摘要翻译： 本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。 在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器 板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。 本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件 大量死体积的样品成分。 此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。 此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。 本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

    Purification apparatus and method
    6.
    发明授权
    Purification apparatus and method 失效
    净化装置及方法

    公开(公告)号：US06419827B1

    公开(公告)日：2002-07-16

    申请号：US09552301

    申请日：2000-04-18

    申请人： Donald R. Sandell ,  Kevin S. Bodner ,  Mark Borodkin ,  Mark Oldham ,  Jon Hoshizaki

    发明人： Donald R. Sandell ,  Kevin S. Bodner ,  Mark Borodkin ,  Mark Oldham ,  Jon Hoshizaki

    IPC分类号： B01D6300

    CPC分类号： B01L3/50255 ,  B01D61/18 ,  B01L3/5025 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102

    摘要： The invention includes a filtration apparatus for processing a plurality of fluid samples into sample wells is provided. In one embodiment, the filtration apparatus includes a purification tray and a sample well tray with a plurality of sample wells. The purification tray includes a filter plate having a plurality of columns with discharge openings at the bottom thereof, at least one filter positioned in the columns of the filter plate for filtering the fluid samples as they pass therethrough, a heat plate positioned adjacent the columns of the filter plate, and a vent plate positioned below the heat plate. The heat plate is configured for transferring heat to the columns of the filter plate. The vent plate includes vents for permitting aerosols from the sample wells of the sample well tray to escape. A sample well tray with a plurality of the sample wells is positioned so that the sample wells align with the discharge openings of the filter plate columns to receive the liquid sample therein.

    摘要翻译： 本发明提供了一种用于将多个流体样品加工成样品孔的过滤装置。 在一个实施例中，过滤装置包括净化盘和具有多个样品孔的样品井托盘。 净化盘包括一个过滤板，该过滤板具有多个在其底部具有排放口的柱，至少一个过滤器，定位在过滤板的列中，用于在流体样品通过过滤器时对其进行过滤， 过滤板和位于加热板下方的通风板。 加热板被配置为将热量传递到过滤板的列。 通风板包括用于允许来自样品井托盘的样品井的气溶胶逸出的通风口。 定位具有多个样品孔的样品孔盘，使得样品孔与过滤板柱的排出口对准以在其中接收液体样品。

    Manually-operable multi-well microfiltration apparatus and method
    10.
    发明授权
    Manually-operable multi-well microfiltration apparatus and method 有权
    手动操作的多孔微滤装置及方法

    公开(公告)号：US06896849B2

    公开(公告)日：2005-05-24

    申请号：US10104335

    申请日：2002-03-22

    申请人： Mark T. Reed ,  Jon Hoshizaki

    发明人： Mark T. Reed ,  Jon Hoshizaki

    IPC分类号： B01D61/18 ,  B01L3/00 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10

    CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102

    摘要： A multi-well microfiltration apparatus and method are provided and feature a manual touch-off system for transferring pendent drops hanging from discharge-conduits of a discharge-conduit array to respective receiving wells or receiving holes of a corresponding receiving array, with minimum or no cross-contamination between the discharge conduits, or the receiving wells or receiving holes. The manual touch-off is achieved by manually shifting a carriage that supports one of the arrays, into a position whereat pendent drops of fluid hanging from the distal ends of the discharge conduits contact the inner sidewalls of the corresponding receiving wells or receiving holes of the receiving array.

    摘要翻译： 提供了一种多孔微滤装置和方法，其特征在于一种手动触发系统，用于将从排放管阵列的排放管道悬挂的悬挂液滴转移到相应接收孔的相应接收孔或接收孔的最小或不 排放管道或接收井或接收孔之间的交叉污染。 手动触发是通过将支撑其中一个阵列的托架手动地移动到从排出管道的远端悬挂的悬挂液滴与相应的接收井的内侧壁接触的位置或接收孔的接收孔 接收阵列。